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1.
Bioinspir Biomim ; 18(3)2023 04 18.
Artículo en Inglés | MEDLINE | ID: mdl-36863018

RESUMEN

Nowadays, there is a growing awareness on the social and economic importance of the ocean. In this context, being able to carry out a diverse range of operations underwater is of paramount importance for many industrial sectors as well as for marine science and to enforce restoration and mitigation actions. Underwater robots allowed us to venture deeper and for longer time into the remote and hostile marine environment. However, traditional design concepts such as propeller driven remotely operated vehicles, autonomous underwater vehicles, or tracked benthic crawlers, present intrinsic limitations, especially when a close interaction with the environment is required. An increasing number of researchers are proposing legged robots as a bioinspired alternative to traditional designs, capable of yielding versatile multi-terrain locomotion, high stability, and low environmental disturbance. In this work, we aim at presenting the new field of underwater legged robotics in an organic way, discussing the prototypes in the state-of-the-art and highlighting technological and scientific challenges for the future. First, we will briefly recap the latest developments in traditional underwater robotics from which several technological solutions can be adapted, and on which the benchmarking of this new field should be set. Second, we will the retrace the evolution of terrestrial legged robotics, pinpointing the main achievements of the field. Third, we will report a complete state of the art on underwater legged robots focusing on the innovations with respect to the interaction with the environment, sensing and actuation, modelling and control, and autonomy and navigation. Finally, we will thoroughly discuss the reviewed literature by comparing traditional and legged underwater robots, highlighting interesting research opportunities, and presenting use case scenarios derived from marine science applications.


Asunto(s)
Robótica , Robótica/métodos , Biomimética/métodos , Locomoción
2.
J Neurosci Methods ; 370: 109491, 2022 Mar 15.
Artículo en Inglés | MEDLINE | ID: mdl-35101524

RESUMEN

BACKGROUND: Coupling transcranial magnetic stimulation with electroencephalography (TMS-EEG) allows recording the EEG response to a direct, non-invasive cortical perturbation. However, obtaining a genuine TMS-evoked EEG potential requires controlling for several confounds, among which a main source is represented by the auditory evoked potentials (AEPs) associated to the TMS discharge noise (TMS click). This contaminating factor can be in principle prevented by playing a masking noise through earphones. NEW METHOD: Here we release TMS Adaptable Auditory Control (TAAC), a highly flexible, open-source, Matlab®-based interface that generates in real-time customized masking noises. TAAC creates noises starting from the stimulator-specific TMS click and tailors them to fit the individual, subject-specific click perception by mixing and manipulating the standard noises in both time and frequency domains. RESULTS: We showed that TAAC allows us to provide standard as well as customized noises able to effectively and safely mask the TMS click. COMPARISON WITH EXISTING METHODS: Here, we showcased two customized noises by comparing them to two standard noises previously used in the TMS literature (i.e., a white noise and a noise generated from the stimulator-specific TMS click only). For each, we quantified the Sound Pressure Level (SPL; measured by a Head and Torso Simulator - HATS) required to mask the TMS click in a population of 20 healthy subjects. Both customized noises were effective at safe (according to OSHA and NIOSH safety guidelines) and lower SPLs with respect to standard noises. CONCLUSIONS: At odds with previous methods, TAAC allows creating effective and safe masking noises specifically tailored on each TMS device and subject. The combination of TAAC with tools for the real-time visualization of TEPs can help control the influence of auditory confounds also in non-compliant patients. Finally, TAAC is a highly flexible and open-source tool, so it can be further extended to meet different experimental requirements.


Asunto(s)
Electroencefalografía , Estimulación Magnética Transcraneal , Electroencefalografía/métodos , Potenciales Evocados/fisiología , Potenciales Evocados Auditivos/fisiología , Voluntarios Sanos , Humanos , Estimulación Magnética Transcraneal/métodos
3.
Neurogastroenterol Motil ; 30(9): e13346, 2018 09.
Artículo en Inglés | MEDLINE | ID: mdl-29644781

RESUMEN

BACKGROUND: Achalasia is a rare motility disorder characterized by myenteric neuron and interstitial cells of Cajal (ICC) abnormalities leading to deranged/absent peristalsis and lack of relaxation of the lower esophageal sphincter. The mechanisms contributing to neuronal and ICC changes in achalasia are only partially understood. Our goal was to identify novel molecular features occurring in patients with primary achalasia. METHODS: Esophageal full-thickness biopsies from 42 (22 females; age range: 16-82 years) clinically, radiologically, and manometrically characterized patients with primary achalasia were examined and compared to those obtained from 10 subjects (controls) undergoing surgery for uncomplicated esophageal cancer (or upper stomach disorders). Tissue RNA extracted from biopsies of cases and controls was used for library preparation and sequencing. Data analysis was performed with the "edgeR" option of R-Bioconductor. Data were validated by real-time RT-PCR, western blotting and immunohistochemistry. KEY RESULTS: Quantitative transcriptome evaluation and cluster analysis revealed 111 differentially expressed genes, with a P ≤ 10-3 . Nine genes with a P ≤ 10-4 were further validated. CYR61, CTGF, c-KIT, DUSP5, EGR1 were downregulated, whereas AKAP6 and INPP4B were upregulated in patients vs controls. Compared to controls, immunohistochemical analysis revealed a clear increase in INPP4B, whereas c-KIT immunolabeling resulted downregulated. As INPP4B regulates Akt pathway, we used western blot to show that phospho-Akt was significantly reduced in achalasia patients vs controls. CONCLUSIONS & INFERENCES: The identification of altered gene expression, including INPP4B, a regulator of the Akt pathway, highlights novel signaling pathways involved in the neuronal and ICC changes underlying primary achalasia.


Asunto(s)
Acalasia del Esófago/metabolismo , Monoéster Fosfórico Hidrolasas/biosíntesis , Proteínas Proto-Oncogénicas c-kit/biosíntesis , Adolescente , Adulto , Anciano , Anciano de 80 o más Años , Regulación hacia Abajo , Femenino , Humanos , Células Intersticiales de Cajal/metabolismo , Masculino , Persona de Mediana Edad , Neuronas/metabolismo , Transcriptoma , Adulto Joven
4.
Clin Sarcoma Res ; 7: 1, 2017.
Artículo en Inglés | MEDLINE | ID: mdl-28078078

RESUMEN

BACKGROUND: Gastrointestinal stromal tumor (GIST) is the most common mesenchymal neoplasm of the gastrointestinal tract. The main treatment for localized gastrointestinal stromal tumors is surgical resection. Unresectable or advanced GIST are poorly responsive to conventional cytotoxic chemotherapy but the introduction of tyrosine kinase inhibitors (TKIs) marked a revolutionary step in the treatment of these patients, radically improving prognosis and clinical benefit. Historically GIST has been considered radiation-resistant, and the role of radiotherapy in the management of patients with GIST is currently restricted to symptomatic palliation in current treatment guidelines. CASE PRESENTATION: Here we report two patients affected by metastatic GIST, treated with radiotherapy and radiosurgery in combination with TKIs, achieving an unexpected objective response in the first case and a significant clinical benefit associated with a local tumor control of several months in the second case. CONCLUSIONS: These and other successful experiences that are progressively accumulating, open up new scenarios of use of radiation therapy in various settings of treatment. GIST is not universally radioresistant and radiotherapy, especially if combined with molecularly targeted therapy, can improve the outcomes for patients diagnosed with GIST.

5.
Eur J Cancer ; 50(9): 1657-64, 2014 Jun.
Artículo en Inglés | MEDLINE | ID: mdl-24703573

RESUMEN

BACKGROUND: Extraskeletal myxoid chondrosarcoma (EMC) is a rare soft tissue sarcoma, marked by NR4A3 rearrangement. Herein we report on the activity of sunitinib in a series of 10 patients, strengthening what initially observed in two cases. PATIENTS AND METHODS: From July 2011, 10 patients with progressive metastatic translocated EMC have been consecutively treated with sunitinib 37.5mg/day, on a named-use basis. In an attempt to interpret the activity of sunitinib in EMC, genotype/phenotype correlations were carried out by fluorescence in situ hybridization (FISH) analyses. Moreover, transcriptome, immunohistochemical and biochemical analyses of a limited set of samples were performed focusing on some putative targets of sunitinib. RESULTS: Eight of 10 patients are still on therapy. Six patients had a Response Evaluation Criteria in Solid Tumours (RECIST) partial response (PR), two were stable, two progressed. Positron emission tomography (PET) was consistent in 6/6 evaluable cases. One patient underwent surgery after sunitinib, with evidence of a pathologic response. At a median follow-up of 8.5 months (range 2-28), no secondary resistance was detected. Median progression free survival (PFS) has not been reached. Interestingly, all responsive cases turned out to express the typical EWSR1-NR4A3 fusion, while refractory cases carried the alternative TAF15-NR4A3 fusion. Among putative sunitinib targets, only RET was expressed and activated in analysed samples. CONCLUSIONS: This report confirms the therapeutic activity of sunitinib in EMC. Genotype/phenotype analyses support a correlation between response and EWSR1-NR4A3 fusion. Involvement of RET deserves further investigation.


Asunto(s)
Inhibidores de la Angiogénesis/uso terapéutico , Antineoplásicos/uso terapéutico , Neoplasias Óseas , Condrosarcoma/tratamiento farmacológico , Indoles/uso terapéutico , Neoplasias de los Tejidos Conjuntivo y Blando/tratamiento farmacológico , Pirroles/uso terapéutico , Adulto , Anciano , Proteínas de Unión a Calmodulina/genética , Condrosarcoma/genética , Condrosarcoma/secundario , Proteínas de Unión al ADN/genética , Resistencia a Antineoplásicos/genética , Femenino , Reordenamiento Génico/genética , Genotipo , Humanos , Masculino , Persona de Mediana Edad , Neoplasias de los Tejidos Conjuntivo y Blando/genética , Neoplasias de los Tejidos Conjuntivo y Blando/secundario , Fenotipo , Proteínas Proto-Oncogénicas c-kit/efectos de los fármacos , Proteínas Proto-Oncogénicas c-ret/efectos de los fármacos , Proteína EWS de Unión a ARN , Proteínas de Unión al ARN/genética , Receptor beta de Factor de Crecimiento Derivado de Plaquetas/efectos de los fármacos , Receptores de Esteroides/genética , Receptores de Hormona Tiroidea/genética , Sunitinib , Factores Asociados con la Proteína de Unión a TATA/genética , Resultado del Tratamiento , Receptor 2 de Factores de Crecimiento Endotelial Vascular/efectos de los fármacos , Tirosina Quinasa 3 Similar a fms/efectos de los fármacos
7.
Artículo en Inglés | MEDLINE | ID: mdl-25569964

RESUMEN

We investigate the phenomenon of HIV eradication at the early stage of the infection and evaluate the chance of the eradication with a mathematical model. We employ an extracellular deterministic model of the HIV infection dynamics and modify the model to include the pharmacokinetics and pharmacodynamics of antiretroviral HIV drugs. In addition we consider clinical experiments for the prevention of HIV infection using pre-exposure chemoprophylaxis treatment. Exploiting the mathematical model we implement the experiment numerically. The study in this paper is supported by the clinical results and provides a theoretical explanation for the results. The result suggests that the protocol of the experiment eradicates the virus in HIV infected patients.


Asunto(s)
Erradicación de la Enfermedad , Infecciones por VIH/tratamiento farmacológico , Modelos Biológicos , Fármacos Anti-VIH/farmacocinética , Fármacos Anti-VIH/farmacología , Fármacos Anti-VIH/uso terapéutico , Simulación por Computador , Infecciones por VIH/virología , Humanos , Análisis Numérico Asistido por Computador
8.
Pharmacogenomics J ; 13(4): 335-41, 2013 Aug.
Artículo en Inglés | MEDLINE | ID: mdl-22584460

RESUMEN

Genetic heterogeneity in drug-metabolizing enzyme/transporter (DMET) genes affects specific drug-related cancer phenotypes. To investigate the relationships between genetic variation and response to treatment in acute myeloid leukemia (AML), we genotyped 1931 variants on DMET genes in 94 CD33-positive AML patients enrolled in a phase III multicenter clinical trial combining Gemtuzumab-Ozogamicin (GO) with Fludarabine-Cytarabine-Idarubicin (FLAI) regimen, with the DMET Plus platform. Two ADH1A variants showed statistically significant differences (odds ratio (OR)=5.68, P=0.0006; OR=5.35, P=0.0009) in allele frequencies between patients in complete/partial remission and patients without response, two substitutions on CYP2E1 (OR=0.13, P=0.001; OR=0.09, P=0.003) and one on SLCO1B1 (OR=4.68, P=0.002) were found to differently influence liver toxicity, and two nucleotide changes on SULTB1 and SLC22A12 genes correlated with response to GO (OR=0.24, P=0.0009; OR=2.75, P=0.0029). Genetic variants were thus found for the first time to be potentially associated with differential response and toxicity in AML patients treated with a combination of GO-FLAI regimen.


Asunto(s)
Enzimas/genética , Inactivación Metabólica/genética , Leucemia Mieloide Aguda/tratamiento farmacológico , Leucemia Mieloide Aguda/genética , Aminoglicósidos/administración & dosificación , Anticuerpos Monoclonales Humanizados/administración & dosificación , Citarabina/administración & dosificación , Enzimas/metabolismo , Femenino , Gemtuzumab , Heterogeneidad Genética , Genotipo , Humanos , Idarrubicina/administración & dosificación , Leucemia Mieloide Aguda/patología , Masculino , Recurrencia Local de Neoplasia/tratamiento farmacológico , Lectina 3 Similar a Ig de Unión al Ácido Siálico/genética , Resultado del Tratamiento , Vidarabina/administración & dosificación , Vidarabina/análogos & derivados
9.
Br J Cancer ; 107(8): 1433-41, 2012 Oct 09.
Artículo en Inglés | MEDLINE | ID: mdl-22929880

RESUMEN

BACKGROUND: Prognosis of localised gastrointestinal stromal tumour (GIST) is heterogeneous, notably for patients with AFIP intermediate or high risk of relapse, who are candidates to adjuvant imatinib. We hypothesised that gene expression profiles might improve the prognostication and help to refine the indications for imatinib. METHODS: We collected gene expression and histoclinical data of 146 pre-treatment localised GIST samples treated with surgery alone. We searched for a gene expression signature (GES) predictive for relapse-free survival (RFS) and compared its performances to that of three published prognostic proliferation-based GES (Genomic Grade Index (GGI), 16-Kinase, and CINSARC) and AFIP classification. We also analysed a data set from 28 patients with advanced GIST treated with neo-adjuvant imatinib. RESULTS: We identified a 275-gene GES (gene expression signature) predictive of RFS in a learning set and validated its robustness in an independent set. However, the GGI outperformed its prognostic performances, and those of the two other signatures and the AFIP intermediate-risk classification in two independent tests sets in uni- and multivariate analyses. Importantly, GGI could split the AFIP intermediate/high-risk samples into two groups with different RFS. Genomic Grade Index 'high-risk' tumours were more proliferative and genetically unstable than 'low-risk' tumours, and more sensitive to imatinib. CONCLUSION: GGI refines the prediction of RFS in localised GIST and might help tailor adjuvant imatinib.


Asunto(s)
Tumores del Estroma Gastrointestinal/genética , Antineoplásicos/uso terapéutico , Benzamidas , Femenino , Tumores del Estroma Gastrointestinal/tratamiento farmacológico , Tumores del Estroma Gastrointestinal/cirugía , Perfilación de la Expresión Génica , Humanos , Mesilato de Imatinib , Masculino , Persona de Mediana Edad , Análisis de Secuencia por Matrices de Oligonucleótidos , Piperazinas/uso terapéutico , Pronóstico , Pirimidinas/uso terapéutico , Resultado del Tratamiento
10.
Meat Sci ; 88(4): 604-9, 2011 Aug.
Artículo en Inglés | MEDLINE | ID: mdl-21414728

RESUMEN

A pilot study using Affymetrix Gene Chip(®) Porcine Genome Arrays was set up to evaluate the impact of time lags from death on gene expression profiling of porcine skeletal muscle at four post mortem times (up to 24h) during the routine processing of fresh thighs. All post chip parameters and data analyses (Average background, Scale Factors, Percent Presence, 3'/5' ratios of ß-actin and glyceraldehyde-3-phosphase dehydrogenase control genes, RNA degradation diagnostics, principal component analysis, hierarchical clustering, mixed regression models with time effects) did not show any effect of post mortem time. Therefore, microarray data obtained from muscle specimens collected in a processing plan over a quite long period have the potential to identify treatments or pre mortem conditions without any potential bias derived from subtle RNA degradation. These results open new perspectives to develop and analyse gene expression biomarkers for pig production and product authentication.


Asunto(s)
Expresión Génica , Carne/análisis , Análisis por Micromatrices/métodos , Músculo Esquelético/metabolismo , Porcinos/fisiología , Actinas/genética , Animales , Femenino , Perfilación de la Expresión Génica , Modelos Lineales , Proyectos Piloto , Análisis de Componente Principal , Estabilidad del ARN , ARN Mensajero/aislamiento & purificación , ARN Mensajero/metabolismo
11.
Oncogene ; 30(24): 2730-40, 2011 Jun 16.
Artículo en Inglés | MEDLINE | ID: mdl-21278796

RESUMEN

Identification of patient selection criteria and understanding of the potential mechanisms involved in the development of resistance are crucial for an appropriate and successful design of clinical trials with anti-insulin-like growth factor (IGF)-1R therapies. Few Ewing's sarcomas are highly sensitive to IGF-1R targeting and understanding the reason why, may hold the secret to improve successful treatments. In this paper, we show that a major mechanism of resistance to highly specific inhibitors of IGF-1R, either antibodies or tyrosine kinase inhibitors may involve enhanced insulin receptor (IR)-A homodimer formation and IGF-2 production. Resistant cells are able to switch from IGF-1/IGF-1R to IGF-2/IR-A dependency to maintain sustained activation of AKT and ERK1/2, proliferation, migration and metastasis. These cells also showed higher proliferative response to insulin, in keeping with a switch towards insulin pathways sustaining proliferation and malignancy, rather than metabolism. Our findings demonstrate a role for IR-A in eliciting intrinsic and adaptive resistance to anti-IGF-1R therapies. Thus, we indicate that tumors with low IGF-1R:IR ratio are unlikely to greatly benefit from anti-IGF-1R therapies and that the efficacy of anti-IGF-1R therapies should be evaluated in relationship to the IR-A:IGF-1R ratio in cancer cells. Moreover, we provide evidences supporting IR-A as an important target in sarcoma therapy.


Asunto(s)
Anticuerpos Monoclonales/farmacología , Receptor IGF Tipo 1/antagonistas & inhibidores , Receptor de Insulina/fisiología , Sarcoma de Ewing/tratamiento farmacológico , Transducción de Señal/fisiología , Animales , Línea Celular Tumoral , Resistencia a Antineoplásicos , Femenino , Humanos , Ratones , Receptor IGF Tipo 1/análisis , Receptor de Insulina/análisis
12.
G Ital Med Lav Ergon ; 33(3 Suppl): 122-5, 2011.
Artículo en Italiano | MEDLINE | ID: mdl-23393818

RESUMEN

Teachers are at a higher risk for occupation-related voice disorders than the general population. The aim of this work is the investigations on the relationships between vocal doses and clinical status, acoustical conditions (noise and reverberation) in the classrooms and subjective evaluations of vocal effort and fatigue. At clinical evaluation 41% of the teachers showed no sign of vocal disease. Students talking in the classroom is the most annoying, noise source in classrooms. The consequence of the poor acoustics in all the schools was the decrease in students question perception. The main cause of a vocal pathology was time dose.


Asunto(s)
Docentes , Ruido en el Ambiente de Trabajo/efectos adversos , Enfermedades Profesionales/etiología , Trastornos de la Voz/etiología , Adulto , Fatiga/etiología , Femenino , Humanos , Masculino , Persona de Mediana Edad
13.
Artículo en Inglés | MEDLINE | ID: mdl-21095900

RESUMEN

We investigate a model-based control method to boost the immune response. We apply this control method to select the appropriate immune response between the Th1 and Th2 responses. The idea of state jump is discussed using hybrid models notation. To implement the control idea we propose physically available methods.


Asunto(s)
Venenos de Abeja/administración & dosificación , Abejas/inmunología , Desensibilización Inmunológica/métodos , Hipersensibilidad/prevención & control , Inmunoterapia/métodos , Mordeduras y Picaduras de Insectos/tratamiento farmacológico , Modelos Inmunológicos , Terapia Asistida por Computador/métodos , Animales , Venenos de Abeja/inmunología , Simulación por Computador , Humanos , Hipersensibilidad/inmunología , Mordeduras y Picaduras de Insectos/complicaciones , Mordeduras y Picaduras de Insectos/inmunología
14.
G Chir ; 31(5): 229-32, 2010 May.
Artículo en Inglés | MEDLINE | ID: mdl-20615365

RESUMEN

Anatomical variations of the cystic duct are well-defined. The presence of short or absent cystic duct is unusual and represents a co-factor of biliary injury especially during laparoscopic cholecystectomy. Thus, its knowledge is important to avoid ductal injury in hepato-biliary surgery. We experienced the case of a 40-year-old woman with symptomatic cholelitiasis, who underwent to laparoscopic cholecystectomy. At surgery, an accidental bile duct lesion was carried, during Calot's triangle dissection, due the particular difficulties in dissecting an extremely short cystic duct found at the junction of the common hepatic duct and common bile duct. No vascular anomalies were present. The biliary leakage from the common bile duct was intraoperative identified and subsequentially treated by the endoscopic method. Laparoscopic cholecystectomy with sequential biliary endoprosthesis insertion was completed without conversion to open surgery. The endoscopic stenting was the definitive treatment for the leakage. No evidence of biliary stent complication was observed during the follow-up. This report documents a case of short cystic duct with particular emphasis to the biliary injury risk during the laparoscopic dissection of "unusual" Calot's triangle, and examines our mini-invasive therapeutic strategies in the management of bile leakage after laparoscopic cholecystectomy.


Asunto(s)
Colecistectomía Laparoscópica/efectos adversos , Conducto Cístico/lesiones , Conducto Cístico/cirugía , Complicaciones Intraoperatorias , Stents , Adulto , Fuga Anastomótica/etiología , Fuga Anastomótica/cirugía , Colecistectomía Laparoscópica/métodos , Colelitiasis/cirugía , Conducto Cístico/anomalías , Endoscopía/métodos , Femenino , Humanos , Complicaciones Intraoperatorias/cirugía , Reoperación , Resultado del Tratamiento
16.
Artículo en Inglés | MEDLINE | ID: mdl-19963607

RESUMEN

We study the differential equations describing the chronic myeloid leukaemia. We propose a novel drug scheduling method to enhance the T-cell mediated immune response. The control strategy relies on the understanding of the immune boosting mechanism. The feasibility of the strategy is illustrated via simulations.


Asunto(s)
Antineoplásicos/administración & dosificación , Esquema de Medicación , Quimioterapia/métodos , Sistema Inmunológico/efectos de los fármacos , Leucemia Mielógena Crónica BCR-ABL Positiva/tratamiento farmacológico , Leucemia Mielógena Crónica BCR-ABL Positiva/inmunología , Linfocitos T/inmunología , Algoritmos , Vacunas contra el Cáncer , Biología Computacional/métodos , Simulación por Computador , Humanos , Activación de Linfocitos/efectos de los fármacos , Modelos Estadísticos , Factores de Tiempo
18.
Hum Gene Ther ; 20(5): 453-64, 2009 May.
Artículo en Inglés | MEDLINE | ID: mdl-19215191

RESUMEN

The highly aggressive cancer syndrome of female mice carrying a p53 knockout allele and a rat HER-2/neu (Neu) transgene (BALB-p53Neu) can be prevented by a cell vaccine presenting three components: Neu, interleukin (IL)-12 production, and allogeneic major histocompatibility complex (MHC) alleles (Triplex cell vaccine). Here we tested a second-generation Triplex DNA-based vaccine (Tri-DNA), consisting of the combination of three gene components (a transmembrane-extracellular domain fragment of the Neu gene, IL-12 genes, and the H-2D(q) allogeneic MHC gene), carried by separate plasmids. The Tri-DNA vaccine was at least as effective as the Triplex cell vaccine for cancer immunoprevention, giving a similar delay in the onset of mammary cancer and complete protection from salivary cancer. Both vaccines induced anti-Neu antibodies of the murine IgG2a isotype at similar levels. The Tri-DNA vaccine gave more restricted immunostimulation, consisting of a fully helper T cell type 1 (Th1)-polarized response, with effective production of interferon (IFN)-gamma in response to the vaccine but no spontaneous production, and no induction of anti-Neu IgG3 antibodies. On the other hand, the Triplex cell vaccine induced both Th1 and Th2 cytokines, a strong increase in spontaneous IFN-gamma production, and high levels of IgG3 antibodies recognizing Neu-positive syngeneic cells. In conclusion, the Tri-DNA vaccine is as effective as Triplex cell vaccine, exploiting a more restricted immune stimulation.


Asunto(s)
Vacunas contra el Cáncer/inmunología , Interleucina-12/inmunología , Síndromes Neoplásicos Hereditarios/prevención & control , Receptor ErbB-2/genética , Proteína p53 Supresora de Tumor/genética , Vacunas de ADN/inmunología , Animales , Citocinas/biosíntesis , Citocinas/inmunología , Citotoxicidad Inmunológica , Femenino , Terapia Genética , Inmunoglobulina G/sangre , Inmunoterapia , Interferón gamma/biosíntesis , Interferón gamma/inmunología , Interleucina-12/metabolismo , Complejo Mayor de Histocompatibilidad/inmunología , Glándulas Mamarias Animales/inmunología , Glándulas Mamarias Animales/patología , Ratones , Síndromes Neoplásicos Hereditarios/terapia , Ratas , Receptor ErbB-2/inmunología , Receptor ErbB-2/metabolismo , Glándulas Salivales/inmunología , Glándulas Salivales/patología , Transfección , Proteína p53 Supresora de Tumor/inmunología , Proteína p53 Supresora de Tumor/metabolismo
19.
Br J Cancer ; 99(10): 1729-34, 2008 Nov 18.
Artículo en Inglés | MEDLINE | ID: mdl-18827815

RESUMEN

At present no reports on gene expression profiling of liver metastases from colorectal cancer are available. We identified two different signatures using Affymetrix platform: epidermal growth factor receptor pathway was upregulated in metachronous lesions, whereas the pathway mainly related to angiogenesis was in synchronous lesions. Synchronous or metachronous liver metastases could be treated differently on the basis of different molecular pathways.


Asunto(s)
Neoplasias Colorrectales/genética , Perfilación de la Expresión Génica , Neoplasias Hepáticas/genética , Adulto , Anciano , Biomarcadores de Tumor/genética , Neoplasias Colorrectales/patología , Receptores ErbB/genética , Femenino , Humanos , Neoplasias Hepáticas/secundario , Masculino , Persona de Mediana Edad , Neoplasias Primarias Múltiples/genética , Neoplasias Primarias Secundarias/genética
20.
Artículo en Inglés | MEDLINE | ID: mdl-19163825

RESUMEN

The human immunodeficiency virus (HIV) infection, that causes acquired immune deficiency syndrome (AIDS), is a dynamic process that can be modeled via differential equations. In this paper we apply a control strategy to boost the immune response for a tristable HIV dynamic model. The purpose of this control method is to steer the system to an equilibrium condition known as long-term nonprogressor, which corresponds to an infected patient that does not develop the symptoms of AIDS. The control strategy is implemented by controlled drug scheduling based on the understanding of the immune boosting mechanism. The feasibility of the methodology is illustrated via simulations.


Asunto(s)
Fármacos Anti-VIH/administración & dosificación , Linfocitos T CD4-Positivos/inmunología , Quimioterapia Asistida por Computador/métodos , Infecciones por VIH/tratamiento farmacológico , Infecciones por VIH/inmunología , Sobrevivientes de VIH a Largo Plazo , Modelos Inmunológicos , Simulación por Computador , Retroalimentación , Humanos , Factores de Tiempo
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