Health-related quality of life in reproductive-age women on antiretroviral therapy: a cross sectional study in Ethiopia.

OBJECTIVES: While numerous studies have explored the impact of gender on Health-Related Quality of Life (HRQOL), there is a lack of evidence specifically among reproductive-age women undergoing Antiretroviral Therapy (ART). Therefore, this study aims to investigate HRQOL and its associated factors among reproductive-age women receiving ART at Hiwot Fana Comprehensive Specialized Hospital in Eastern Ethiopia. METHODS: A cross-sectional study included 418 women of ART. The World Health Organization quality of Life Questionnaire (WHOQOL-HIV BREF) was used to assess HRQOL. Binary logistic regression was used to determine the factors that could predict HRQOL. RESULTS: The study found that the median age of the participants was 37 years, and the overall percentage of women with good HRQOL was 40.7%. Women who stayed on ART for more than 6 years had higher odds of overall good HRQOL (AOR 6.73, 95% CI 3.31-13.71) compared to those with a duration of 6 years or less. Besides, women having no child (AOR 25.03, 95% CI 4.93-127.06), one child (AOR 18.60, 95% CI 3.95-87.65), two children (AOR 12.89, 95% CI 3.66-45.37) and three children (AOR 3.77, 95% CI 1.06-13.34) had higher odds of overall good HRQOL compared to those with four/more children. CONCLUSIONS: The study found that the majority of women on ART had poor HRQOL. Factors such as longer duration of taking ART, higher CD4+ cell count, disclosure of HIV status, and receiving care from adherence support teams were associated with better quality of life. Thus, strengthening adherence support teams, promoting safe disclosure of HIV status, and providing comprehensive support for HIV-positive women are required to improve their overall HRQOL.

In vitro Antibacterial Activities of Selected Medicinal Plants Used by Traditional Healers for Treating Urinary Tract Infection in Haramaya District, Eastern Ethiopia.

Background: Despite the presence of antibacterial agents for urinary tract infection treatment, most of the uropathogenic bacteria reveal multi-drug resistance. Health and economic loss due to these represent a rising burden worldwide which necessitates serious action at regional, national and global levels. Thus, alternative approaches to overcome this problem by using bioactive compounds from traditional medicinal plants are required. This study was designed to evaluate the in-vitro antibacterial activity of Punica granatum fruit peels, Nigella sativa seeds, and Echinops kebericho used in the traditional treatment of urinary tract infections. Methods: An experimental study was employed to evaluate the in vitro antibacterial activity of methanol and ethanol crude extract of Punica granatum fruit peels, Nigella sativa seeds, and Echinops kebericho roots of six dilutions (25, 50, 100, 125, 250, and 500) mg/mL. Disc diffusion and macro broth dilution methods were used to determine antimicrobial activity test and minimum inhibitory concentration respectively against E. coli, P. aeruginosa, K. pneumoniae, P. mirabilis, and S. aureus bacterias. Results: Antibacterial activities of ethanol and methanol crude extract of Punica granatum fruit peels against E. coli ATCC25922, P. aeruginosa ATCC27853, S. aureus ATCC25923, K. pneumoniae UK5099 and P. mirabilis UK5999 had highest inhibition zones among tested plants. All tested bacteria were highly sensitive to Punica granatum extract. The second most active plant extract in inhibiting the growth of tested bacteria was Nigella sativa while Echinops kebericho showed the smallest efficacy against tested bacteria. The inhibition zone diameter produced by the methanol extract of each screened plant had higher inhibition zones than ethanol extract. Conclusion: The crude extracts of Punica granatum fruit peels, Nigella sativa seeds, and Echinops kebericho roots have promising antibacterial activity against tested uropathogenic bacteria.

Follow-up conditions of care and associated factors among adult hypertensive patients during COVID-19 at West Arsi public health facilities, Southeastern Ethiopia: A multi-center cross-sectional study.

Background: Coronavirus disease (COVID-19) is a viral-borne infection caused by the SARS-CoV-2 virus. Aside from the morbidity and mortality effects, it leaves the majority of hypertensive patients untreated and vulnerable to uncontrolled hypertension. Objective: The purpose of this study was to assess follow-up conditions of care and its associated factors among adult hypertensive patients during COVID-19 in public health facilities of West Arsi, Southeastern Ethiopia. Methods: A health facility-based retrospective cross-sectional study was conducted among 423 adult hypertensive patients in the West Arsi public health facilities from July 5 to August 6, 2021. A systematic random sampling technique was used to recruit the study participants. A pretested structured face-to-face interviewer and medical records were used to collect sociodemographic variables, basic clinical features, and follow-up data. The follow-up conditions of care were assessed using 12 items with "yes or no" questions. Then, based on the mean value of the items, the follow-up conditions of care were dichotomized into good and poor. As a result, the follow-up condition was good if the score was greater or equal to the mean, and poor unless otherwise. To investigate parameters related with follow-up conditions of care, bivariable and multivariable logistic regression analyses were used. A 95% confidence interval and a p-value of 0.05 were used to indicate a significant association. Results: The rate of poor follow-up conditions of care during COVID-19 was 29% (95% confidence interval: 24.9-33.4%), according to this study. Age ≥ 60 years (AOR = 3.55; 95% CI: 2.09-6.03), transportation problem (AOR = 2.43; 95% CI: 1.28-4.61), fear of COVID-19 (AOR = 3.34; 95% CI: 1.59-7.01), co-morbidity (AOR = 1.93; 95% CI: 1.14-3.26) and physical distancing (AOR = 2.43; 95% CI: 1.44-4.12) were significantly associated with poor follow-up conditions of care. Conclusions: In our study, 29% of the participants had poor follow-up care conditions. When compared to WHO recommendations, the findings of this study may explain poor follow-up care conditions. To enhance patients' follow-up treatment, evidence-based target interventions should be designed and executed, taking into account individuals at high risks, such as those over the age of 60 and those with comorbidities, and identifying additional risk factors.

Prevalence, Antimicrobial Resistance, and Associated Factors of Streptococcus pneumoniae Colonization Rate among Old-Age Patients with Respiratory Tract Infection Attending Sheik Hassan Yebere Referral and Karamara General Hospitals, Jigjiga, Ethiopia.

Background: Streptococcus pneumoniae is part of the normal flora of the upper respiratory tract of humans. Colonization of the upper respiratory tract (carriage of pneumococcus) by S. pneumoniae is considered a prerequisite for pneumococcal infection. It is the major cause of respiratory tract infection and frequent cause of physician visits, hospitalization, and death among old-aged patients because of their low immunity status. However, data on S. pneumoniae among old-aged patients in eastern Ethiopia are limited. This study was undertaken to determine the prevalence, antimicrobial resistance, and associated factor of S. pneumoniae colonization among old-aged patients. Method: A health facility-based cross-sectional study was conducted from 1 March to 15 April 2020, at Sheik Hassan Yebere Referral and Karamara General Hospitals, Jigjiga, eastern Ethiopia. A total of 188 individuals greater than or equal to 60 years suspected of both upper and lower respiratory tract infections were included. Sociodemographic, behavioral, living conditions, and clinical data were collected by trained data collectors. Sputum samples were collected and examined for S. pneumoniae using the culture and biochemical tests as per the standard procedures. The Kirby-Bauer disk diffusion method was used for antimicrobial susceptibility testing. The data were entered on Epi-data version 3.1, and frequencies, crude odds ratio, and adjusted odds ratio were analyzed using SPSS version 20. Results: The prevalence of S. pneumoniae colonization rate among old-aged patients was 13.8% (26/188) (95% CI: 9.6-19.1). Smoking (AOR = 3.3; 95% CI: 1.3-8.3), upper airway problems (AOR = 4.1; 95% CI: 1.1-15), and asthma disease (AOR = 3.1; 95% CI: 1.1-8.9) were the factors associated with S. pneumoniae colonization. The isolated organisms showed high antimicrobial resistance to trimethoprim-sulphamethoxazole (n = 12, 46.2%), tetracycline (n = 11, 42.3%), and ampicillin (n = 9, 34.6%). Conclusion: This study showed that high prevalence of S. pneumoniae and antimicrobial resistance for trimethoprim-sulphamethoxazole, tetracycline, and ampicillin when compared to similar studies. Cigarette smoking, having upper airway problem, and asthma disease were factors associated with S. pneumoniae colonization. The provision of pneumococci conjugate vaccination and avoiding smoking are highly recommended for old aged in the community.

Effect of Regular Khat Chewing on Serum Fasting Sugar Level in Diabetic patients versus Healthy Individuals; A comparative study.

BACKGROUND: Khat chewing is a long standing social-cultural habit in several countries. Even though many people chew khat simply for its pleasurable and stimulatory effect, evidence showed widely-held belief among khat chewers in Ethiopia and other part of the world that khat helps to lower blood glucose while some studies are contradicted on the effect of khat. There is limited data about khat's effect on blood glucose especially in our setting, Harar estern Ethiopia. OBJECTIVE: Primarily the present study aims to compare fasting blood sugar level among khat chewer diabetic and healthy individuals, and to asses risk factors associated with poor glycemic control in diabetic subjects. METHOD: A cross-sectional study included 200 confirmed diabetic and healthy subjects. Fasting blood sugar was determined by enzymatic method glucose oxidase and glucose hexokinase. Glycemic control was also determined for diabetic subjects based on the last 2-month diabetic clinic visits and current measurement. RESULT: (Median ± IQR [interquartile range]) fasting blood sugar difference among Khat chewer and non khat chewer were 159 ± 83 mg/dl and 202 ± 79 mg/dl respectively in diabetic subjects when tested by glucose oxidase. Similarly, in healthy non khat chewer and khat chewer, khat chewers has lower (Median ± IQR) fasting blood glucose level 82 ± 18 mg/dl than non khat chewers 94 ± 13 mg/dl when tested by glucose oxidase. Regarding risk factors associated with poor glycemic control in diabetic subjects, positive parental diabetes history, insulin medication, being overweight, obese were significantly associated with poor glycemic control. CONCLUSION: There was significant effect of khat on median FBS among khat chewers in diabetic and healthy individuals. And the proportion of glycemic control was high among diabetic subjects. RECOMMENDATION: Health care professional and patients should manage the risk factors to delay disease progression and restrain the damage. More studies should be conducted in randomized control trial manner to further elucidate khat effect on blood sugar level so that the actual effect of khat can be identified unlike in cross sectional where there may not be strong causal relationship.

Global prevalence of metabolic syndrome among patients with type I diabetes mellitus: a systematic review and meta-analysis.

BACKGROUND: The presence of metabolic syndrome among diabetes patients is frequent and is associated with an increased incidence of chronic complications and mortality. Despite several studies have been conducted, there is no overall estimation on the prevalence of metabolic syndrome among type 1 diabetic patients. Therefore, this study aimed to estimate the pooled prevalence of metabolic syndrome among patients with type 1 diabetes mellitus. METHODS: Medline via PubMed, CINAHL, ScienceDirect, Ovid, Google Scholar, ResearchGate and African Journals Online were searched by limiting publication period from January 2005 to October 2020. Data were extracted with a standardized format prepared in Microsoft Excel and exported to Stata 16.0 for analyses. The I2 statistic was used to check heterogeneity across the included studies. DerSimonian and Laird random-effects model was applied to estimate pooled prevalence and 95% confidence interval across studies. Funnel plot symmetry, Begg's test and Egger's regression test were used to determine the presence of publication bias. Subgroup and sensitivity analysis as well as meta-regression were conducted to explore the potential sources of heterogeneity. The study protocol is registered on PROSPERO with reference number: CRD42020213435. RESULTS: In this meta-analysis, a total of 27 studies with 45,811 study participants were included. The pooled prevalence of metabolic syndrome was 23.7% with substantial heterogeneity (I2 = 98.2%; P < 0.001). Geographical-based subgroup analysis revealed that the highest prevalence was observed in Australia (27.3%). As per meta-analysis of 17 studies, the pooled prevalence of metabolic syndrome in female type 1 diabetes patients (25.9%) was slightly higher than male T1DM patients (22.5%). CONCLUSION: Nearly a quarter of the type 1 diabetes mellitus patients were affected by metabolic syndrome. Therefore, more attention should be paid to the prevention and control of the epidemic and for the reduction of the morbidity and mortality associated with metabolic syndrome among type 1 diabetes mellitus patients.

Gender Differences in Perceived Stigma and Coping Strategies Among People Living with HIV/AIDS at Jugal Hospital, Harar, Ethiopia.

BACKGROUND: HIV/AIDS is among the most devastating diseases, having multiple effects on the health and well-being of people living with HIV/AIDS (PLWHA). There is a paucity of studies that examined the gender differences in perceived stigma among PLWHA and the different coping strategies that they use in Ethiopia. OBJECTIVE: To assess the gender differences in perceived stigma and coping strategies among PLWHA. METHODS: A comparative cross-sectional study was conducted at Jugal Hospital, Harar, eastern Ethiopia from May 01 to July 30, 2018. A total of 412 (206 females and 206 males) PLWHA were included. Face-to-face interviewer-administered data were collected. Perceived HIV stigma was assessed using the Berger HIV stigma scale. Similarly, the coping strategies were assessed using the Brief Coping Orientation to Problems Experienced (Brief COPE) scale. The data were analyzed using STATA version 13. RESULTS: The mean Berger stigma scale score was 65.3±11.3. The overall perceived stigma mean score was not statistically different between males and females (64.8±10.8 vs 65.8±11.7, p=0.407). Among the four subscales, women reported a higher level of disclosure stigma than men (21.1±5.5 vs 20.3±5.5; p=0.006). Regarding maladaptive coping strategies, men often used substances compared to women (2.8±1.5 vs 2.1±0.4; p<0.001). However, women use behavioral disengagement (4.6±1.1 vs 4.2±1.1; p=0.002) and self-distraction (5.9±1.5 vs 5.5±1.7; p=0.019) more often than men. Females used a higher level of adaptive coping strategies compared to males (42.5±4.9 vs 40.9±6.3, p=0.005). Furthermore, females were found to use more emotional-focused coping than males (27.6±3.2 vs 26.4±4.0; p<0.001). CONCLUSION: Women reported a higher level of disclosure stigma than men and the types of coping strategies used vary between male and female. Thus, interventions to support PLWHA must take into account the gender differences in terms of perceived stigma.

Bacterial otitis media in sub-Saharan Africa: a systematic review and meta-analysis.

BACKGROUND: Otitis media is inflammation of the middle ear, comprising a spectrum of diseases. It is the commonest episode of infection in children, which often occurs after an acute upper respiratory tract infection. Otitis media is ranked as the second most important cause of hearing loss and the fifth global burden of disease with a higher incidence in developing worlds like Sub-Saharan Africa and South Asia. Therefore, this systematic review is aimed to quantitatively estimate the current status of bacterial otitis media, bacterial etiology and their susceptibility profile in sub-Saharan Africa. METHODS: A literature search was conducted from major databases and indexing services including EMBASE (Ovid interface), PubMed/MEDLINE, Google Scholar, ScienceDirect, Cochrane Library, WHO African Index-Medicus and others. All studies (published and unpublished) addressing the prevalence of otitis media and clinical isolates conducted in sub-Saharan Africa were included. Format prepared in Microsoft Excel was used to extract the data and data was exported to Stata version 15 software for the analyses. Der-Simonian-Laird random-effects model at a 95% confidence level was used for pooled estimation of outcomes. The degree of heterogeneity was presented with I2 statistics. Publication bias was presented with funnel plots of standard error supplemented by Begg's and Egger's tests. The study protocol is registered on PROSPERO with reference number ID: CRD42018102485 and the published methodology is available from http://www.crd.york.ac.uk/CRD42018102485. RESULTS: A total of 33 studies with 6034 patients were included in this study. All studies have collected ear swab/discharge samples for bacterial isolation. The pooled isolation rate of bacterial agents from the CSOM subgroup was 98%, patients with otitis media subgroup 87% and pediatric otitis media 86%. A univariate meta-regression analysis indicated the type of otitis media was a possible source of heterogeneity (p-value = 0.001). The commonest isolates were P. aeruginosa (23-25%), S. aureus (18-27%), Proteus species (11-19%) and Klebsiella species. High level of resistance was observed against Ampicillin, Amoxicillin-clavulanate, Cotrimoxazole, Amoxicillin, and Cefuroxime. CONCLUSION: The analysis revealed that bacterial pathogens like P. aeruginosa and S. aureus are majorly responsible for otitis media in sub-Saharan Africa. The isolates have a high level of resistance to commonly used drugs for the management of otitis media.

Trend Analysis And Seasonality Of Tuberculosis Among Patients At The Hiwot Fana Specialized University Hospital, Eastern Ethiopia: A Retrospective Study.

PURPOSE: Tuberculosis (TB) is one of the top 10 leading killer diseases in developing countries, particularly in Sub-Saharan Africa, including Ethiopia. Thus, this study aimed to assess the trend analysis and seasonality of TB at Hiwot Fana Specialized University Hospital, Eastern Ethiopia. METHODS AND PATIENTS: A hospital-based retrospective study was conducted on 8,001 patients by reviewing all available patients' data from January 1, 2015 to April 30, 2019, at the Hiwot Fana Specialized University Hospital, Eastern Ethiopia. Socio-demographic characteristics and results of the GeneXpert assay were taken from the registration book. The data were entered into EpiData 3.1 and analyzed by using the statistical Package for Social Sciences (SPSS) version 20. RESULTS: From a total of 8,001 samples tested using Genexpert, the overall prevalence of Mycobacterium tuberculosis and rifampicin resistance was found to be 1,254 (15.7%) and 53 (4.1%), respectively. A decreasing trend of TB prevalence was observed, and decreased from 19.3% in 2015, 18.6% in 2016, to 18.4% in 2017, 13.5% in 2018 and down to 13.0% in 2019 (P-value<0.001). The maximum number of TB cases were reported during autumn (454, 17.1%) and summer (310, 17.2%) compared to other seasons of all the study period. Being between the ages of 15-29 years (adjusted odds ratio (AOR)=1.7, 95% confidence interval (CI)=1.41-1.98), of male gender (AOR=0.84, 95% CI=0.75-0.96), experiencing a relapse of TB (AOR=0.51, 95% CI=0.35-0.78), and being HIV positive (AOR=0.51, 95% CI=0.3-0.86) were found to be factors associated with high proportion of tuberculosis. CONCLUSION: Prevalence of TB has decreased year to year between January 2015 and April 2019. However, a high percentage of patients are still testing positive for TB with different seasonal variations. Thus, understanding and managing TB in seasonal variation, controlling relapse of TB, and screening of all HIV positive patients are recommended steps to reduce the transmission of tuberculosis in Ethiopia.

Magnitude and causes of first-line antiretroviral therapy regimen changes among HIV patients in Ethiopia: a systematic review and meta-analysis.

BACKGROUND: Antiretroviral therapy (ART) has markedly decreased the morbidity and mortality due to HIV/AIDS. ART regimen change is a major challenge for the sustainability of human immunodeficiency virus (HIV) treatment program. This is found to be a major concern among HIV/AIDS patients in a resource-limited setting, where treatment options are limited. OBJECTIVES: The aim of this review is to generate the best available evidence regarding the magnitude of first-line antiretroviral therapy regimen change and the causes for regimen change among HIV patients on ART in Ethiopia. METHODS: The reviewed studies were accessed through electronic web-based search strategy from PubMed Medline, EMBASE, Hinari, Springer link and Google Scholar. Data were extracted using Microsoft Excel and exported to Stata software version 13 for analyses. The overall pooled estimation of outcomes was calculated using a random-effect model of DerSimonian-Laird method at 95% confidence level. Heterogeneity of studies was determined using I2 statistics. For the magnitude of regimen change, the presence of publication bias was evaluated using the Begg's and Egger's tests. The protocol of this systematic review and meta-analysis was registered in the Prospero database with reference number ID: CRD42018099742. The published methodology is available from: https://www.crd.york.ac.uk/PROSPERO/display_record.php?RecordID=99742 . RESULTS: A total of 22 studies published between the years 2012 and 2018 were included. Out of 22 articles, 14 articles reported the magnitude of regimen change and consisted of 13,668 HIV patients. The estimated national pooled magnitude of regimen change was 37% (95% CI: 34, 44%; Range: 15.1-63.8%) with degree of heterogeneity (I2), 98.7%; p-value < 0.001. Seventeen articles were used to identify the causes for first-line antiretroviral therapy regimen change. The major causes identified were toxicity, 58% (95% CI: 46, 69%; Range: 14.4-88.5%); TB co-morbidity, 12% (95% CI: 8, 16%; Range: 0.8-31.7%); treatment failure, 7% (95% CI: 5, 9%; Range: 0.4-24.4%); and pregnancy, 5% (95% CI: 4, 7%; Range: 0.6-11.9%). CONCLUSIONS: The original first-line regimen was changed in one-third of HIV patients on ART in Ethiopia. Toxicity of the drugs, TB co-morbidity, treatment failure, and pregnancy were the main causes for the change of the first-line regimen among HIV patients on antiretroviral therapy.

Chemical exposure in garage workers and related health risks on the biochemical levels: A comparative study in Harar town, eastern Ethiopia.

OBJECTIVES: Occupational exposure to chemicals causes a wide range of biological effects depending on the level and duration of exposure. The current study is intended to determine the differences in biochemical levels among garage workers compared with occupationally nonexposed participants in Harar town, eastern Ethiopia. METHODS: A comparative cross-sectional study was conducted in Harar town, eastern Ethiopia. Thirty (30) garage workers were selected and compared with 30 age- and sex-matched control group of teachers and students. Demographic and occupational data were collected using a structured questionnaire by trained data collector. Biochemical levels were measured by automated clinical chemistry analyzer (Autolab 18, Boehringer-Mannheim Diagnostics, the United States). Data were analyzed using STATA Version 13. RESULTS: All of the included garage workers were male. A statistically significant increase were found in alanine aminotransferase (35.60 ± 7.93 vs 19.17 ± 0.91 U/L; P value = 0.0440), aspartate aminotransferase (47.23 ± 4.89 vs 27.03 ± 1.13 U/L; P value = 0.0002), total protein (85.83 ± 1.16 vs 76.40 ± 0.86 g/l; P value < 0.0001), uric acid (7.34 ± 0.29 vs 5.19 ± 0.21 mg/dl; P value < 0.0001), glucose (85.13 ± 3.92 vs 75.60 ± 2.40 mg/dl; P value = 0.0425); total cholesterol (199.40 ± 13.11 vs 140.37 ± 3.81 mg/dl; P value = 0.0001) and triglyceride (143.40 ± 5.79 vs 110.60 ± 8.98 mg/dl; P value = 0.0033) in garage workers compared with control group. On the contrary, a statistically significant decrease were found in albumin (39.37 ± 1.78 vs 46.37 ± 0.56 g/l; P value = 0.0004) and urea (21.63 ± 1.04 vs 27.60 ± 1.69 mg/dl; P value = 0.0039) among garage workers compared with the control group. CONCLUSION: Our finding indicates that working in the garage changes most of the biochemical levels. Therefore, appropriate and effective safety measures need to be implemented to prevent possible chemical exposure during routine work.

Resistance profile of clinically relevant bacterial isolates against fluoroquinolone in Ethiopia: a systematic review and meta-analysis.

BACKGROUND: Fluoroquinolones are among the most frequently utilized antibacterial agents in developing countries like Ethiopia. Ciprofloxacin has become the most prescribed drug within this class and remains as one of the top three antibacterial agents prescribed in Ethiopia. However, several studies indicated that there is a gradual increase of antibacterial resistance. Therefore, this meta-analysis aimed to quantitatively estimate the prevalence of ciprofloxacin resistance bacterial isolates in Ethiopia. METHODS: Literature search was conducted from electronic databases and indexing services including EMBASE (Ovid interface), PubMed/MEDLINE, Google Scholar, Science Direct and WorldCat. Data were extracted with structured format prepared in Microsoft Excel and exported to STATA 15.0 software for the analyses. Pooled estimation of outcomes was performed with DerSimonian-Laird random-effects model at 95% confidence level. Degree of heterogeneity of studies was presented with I2 statistics. Publication bias was conducted with comprehensive meta-analysis version 3 software and presented with funnel plots of standard error supplemented by Begg's and Egger's tests. The study protocol has been registered on PROSPERO with reference number ID: CRD42018097047. RESULTS: A total of 37 studies were included for this study. The pooled prevalence of resistance in selected gram-positive bacterial isolates against ciprofloxacin was found to be 19.0% (95% confidence interval [CI]: 15.0, 23.0). The degree of resistance among Staphylococcus aureus, Coagulase negative Staphyloccoci (CoNS), Enterococcus faecalis and Group B Streptococci (GBS) was found to be 18.6, 21.6, 23.9, and 7.40%, respectively. The pooled prevalence of resistance in gram-negative bacteria was about 21.0% (95% CI: 17, 25). Higher estimates were observed in Neisseria gonorrhea (48.1%), Escherichia coli (24.3%) and Klebsiella pneumonia (23.2%). Subgroup analysis indicated that blood and urine were found to be a major source of resistant S. aureus isolates. Urine was also a major source of resistant strains for CoNS, Klebsiella and Proteus species. CONCLUSION: Among gram-positive bacteria, high prevalence of resistance was observed in E. faecalis and CoNS whereas relatively low estimate of resistance was observed among GBS isolates. Within gram-negative bacteria, nearly half of isolates in N. gonorrhoea were found ciprofloxacin resistant. From enterobacteriaceae isolates, K. pneumonia and E. coli showed higher estimates of ciprofloxacin resistance.

Magnitude and associated factors of diabetes mellitus and hypertension among adult HIV-positive individuals receiving highly active antiretroviral therapy at Jugal Hospital, Harar, Ethiopia.

BACKGROUND: People with HIV infection are at increased risk of noncommunicable diseases (NCDs). Diabetes mellitus (DM) and hypertension are recognized as the major NCDs. Except few findings in general population, there is no well-documented evidence on the magnitude of NCDs and associated factors among HIV-positive patients. PURPOSE: The aim of this study was to determine the magnitude and associated factors of DM and hypertension among adult HIV-positive subjects receiving highly active antiretroviral therapy (HAART). METHODS: A hospital-based cross-sectional study was conducted from February to April at Jugal Hospital, Harar, Eastern Ethiopia. Sociodemographic and anthropometric data and blood pressure (BP) were collected by senior clinical nurses. A total of 5 mL of venous blood was collected. Serum glucose and lipid profile were measured using the Autolab 18 clinical chemistry analyzer. Data were analyzed using STATA version 13. RESULTS: A total of 425 HIV-infected individuals taking HAART of age ranging from 18 to 68 years were included. The prevalence of DM and hypertension were 7.1% (95% CI: 4.9-9.9) and 12.7% (95% CI: 9.8-16.2), respectively. Increased blood triglyceride (adjusted odds ratio [AOR] =4.7, 95% CI: 1.7-13.1), high BP (AOR =3.3, 95% CI: 1.1-9.5), and high baseline body mass index (BMI) (AOR =8.7, 95% CI: 2.4-31.8) were significantly associated with DM. In contrast, raised waist-hip ratio (AOR =4.6, 95% CI: 1.6-13.3), raised blood glucose (AOR =3.5, 95% CI: 1.1-11.4), increased total cholesterol (AOR =3.9, 95% CI: 1.3-11.9), high current BMI (AOR =3.8, 95% CI: 1.5-9.6), drinking alcohol (AOR =3.4, 95% CI: 1.5-8.1), CD4 count <500 cell/mL (AOR =2.7, 95% CI: 1.3-5.6), and longer duration of HAART (AOR =2.3, 95% CI: 1.1-5.1) were significantly associated with hypertension. CONCLUSION: DM and hypertension were frequent among HIV patients on HAART, and they were linked to the well-known risk factors. Therefore, regular screening and monitoring of DM and hypertension before and after the initiation of HAART is of paramount importance.

Occupational health risk of working in garages: comparative study on blood pressure and hematological parameters between garage workers and Haramaya University community, Harar, eastern Ethiopia.

BACKGROUND: Occupational exposure to chemicals in garages causes a wide range of biological effects, depending upon the level and duration of exposure. In Ethiopia, there have been few studies conducted to assess the exposure of garage workers to chemicals. Preceding studies have not explored the effect of working in garage on blood pressure and hematological parameters. Therefore, this study aimed to assess differences in blood pressure and hematological parameters among garage workers compared to the Haramaya University community, Harar, eastern Ethiopia. MATERIALS AND METHODS: A comparative cross-sectional study was conducted in Harar town, eastern Ethiopia. Thirty garage workers were selected and compared with 30 age- and sex-matched controls comprising of teachers and students. Demographic and occupational data were collected by using a structured questionnaire by a trained data collector. Blood pressure was measured using sphygmomanometry. Hematological parameters were measured with an automated hematology analyzer. Data were analyzed using Stata version 13. RESULTS: The majority of the garage workers did not implement effective preventive or control measures for workplace chemical exposure. Statistically significant increases were found in systolic (128.67±18.14 vs 106.33 ±9.27 mmHg, P<0.0001), diastolic blood pressure (90.33±11.29 vs 75.67 ±5.68 mmHg, P<0.0001), total white blood cells (7.9±1.51 vs 6.72±2.04×109 cells/L, P=0.0138), and platelets (323.20±48.82 vs 244.1±47.3×109 cells/L, P<0.0001) in garage workers compared to the control group. On the other hand, statistically significant decreases were found in red blood cells (5.13±0.38 vs 5.46±0.36×1012 cells/L, P=0.0006), hemoglobin (14.89±0.71 vs 15.45±0.87 g/dL, P=0.0062), hematocrit (43.98%±1.99% vs 46.4%3±2.32%, P<0.0001), and mean corpuscular volume (83.19±2.93 vs 85.11±3.87 fL, P=0.0353) among garage workers compared to the control group. CONCLUSION: There were significant differences in blood pressure and hematological parameters between garage workers and the control group. Therefore, appropriate and effective safety measures need to be taken by the workers to prevent possible chemical exposure during routine tasks.

Prevalence and Trends of Major Transfusion Transmissible Infections among Blood Donors in Dire Dawa Blood bank, Eastern Ethiopia: Retrospective Study.

BACKGROUND: Acquisition of transfusion transmissible infections in the process of therapeutic blood transfusion is a major global health challenge in transfusion medicine. This study aimed to determine the prevalence and trends of major transfusion transmissible infections among blood donors. METHOD: A retrospective analysis of consecutive blood donors' records covering the period between July 2010 and June 2013 was conducted at Dire Dawa Blood Bank, Eastern Ethiopia. RESULT: A total of 6376 blood donors were tested, out of which 5647(88.57%) were replacement donors and 729(11.43%) were voluntary donors. The majority of them were male, 5430(85.16%), and aged between 18-32 years, 4492(70.45%). A total of 450(7.06%) donors had serological evidence of infection with at least one pathogen. The overall positivity rates of HBV, HIV, HCV and syphilis were 4.67%, 1.24%, 0.96%, and 0.44% respectively. Trends for transfusion-transmissible infections showed a significant decrease from 9.51% in 2010 to 6.95% in 2013 with the least prevalence in 2012 (5.90%) (P = 0.004). The prevalence of transfusion transmissible infections was significantly higher among male blood donors compared to female donors, among the age group of 25-32 years and 33-40 years compared to the age group of 18-24 years old, and among unemployed and private workers compared to students. CONCLUSION: A significant percentage of the blood donors harbor transfusion-transmissible infections. Stringent donor selection using standard methods is highly recommended to ensure the safety of blood for the recipient. Furthermore, efforts on motivating and creating awareness in the community are required to increase voluntary blood donors.