RESUMEN
In this research article, we investigated the effect of Euphorbia bivonae extract compounds on the lethality of brine shrimp Artemia salina and on embryonic cell lines (HEK293) proliferation. Our GC/MS analysis revealed that the E. bivonae ethanolic extract contained essentially sitosterol, euphol, and lupeol. The 24-h LC50 was determined using the probit analysis method (LC50=357.11â mg l-1 ). Depending on this cytotoxicity test result, E. bivona extract induced a significant increase in Superoxide Dismutase (SOD), Catalase (CAT), Glutathione-Peroxidase (GPx) activities, and lipid peroxidation (LPO) in A. salina larvae. In addition, the cytotoxicity effect of this extract had proved against the HEK293â cell lines inâ vitro. We suggest that the three compounds of E. bivonae extract (sitosterol, euphol, and lupeol) are the most responsible for this cytotoxicity. The possible application of this extract as an alternative natural antiproliferative is considered.
Asunto(s)
Euphorbia , Animales , Humanos , Euphorbia/química , Extractos Vegetales/química , Artemia , Células HEK293 , Sitoesteroles/farmacología , Antioxidantes/toxicidad , RiñónRESUMEN
Opuntia stricta is a rich source of phenolic compounds. This species generally has strong antioxidant activities in vitro and in vivo. This study aimed to analyze the antioxidant properties of phenolic compounds isolated from Opuntia stricta, including its radical scavenging activities and preventive action against Cd-induced oxidative stress in rats. To assess the protection of prickly pear juice extract (PPJE) against Cd-induced hepato-nephrotoxicity and testicular damage, male albino rats received PPJE (250 mg kg−1) and/or Cd (1 mg kg−1) by oral administration and injection, respectively, for five consecutive weeks. The preventive action of PPJE was estimated using biochemical markers of kidney and liver tissues, antioxidant status, and histological examinations. In the present study, the lipid peroxidation, protein carbonyls, antioxidant status, and metallothionein levels were determined in different tissues. The chromatographic analysis indicated that PPJE extract is very rich in phenolic compounds such as verbascoside, catechin hydrate, and oleuropein. Our results showed that PPJE-treated rats had significantly (p < 0.05) decreased Cd levels in liver and kidney tissues. In addition, the administration of PPJE induced a significant (p < 0.05) decrease in lipid peroxidation of 30.5, 54.54, and 40.8 in the liver, kidney, and testicle, respectively, and an increase in antioxidant status in these tissues. Additionally, PPJE showed a strong ability to protect renal, hepatic, and testicular architectures against Cd exposure. This study revealed that PPJE protects against the toxic effects of Cd, possibly through its free radical scavenging and antioxidant activities.
Asunto(s)
Opuntia , Animales , Antioxidantes/análisis , Cadmio/toxicidad , Cromatografía Líquida de Alta Presión , Peroxidación de Lípido , Opuntia/química , Estrés Oxidativo , Extractos Vegetales/química , Extractos Vegetales/farmacología , RatasRESUMEN
This study aimed to investigate the microencapsulation of novel condensed tannins isolated from Periploca angustifolia roots, using ß-cyclodextrin macrocyclic oligosaccharides, in order to enhance their antioxidant and antihyperlipidemic potentials. Scanning electron microscopy and Fourier transform infrared spectroscopy results revealed that tannin fraction was successfully included into ß-cyclodextrin cavities proved with an encapsulation efficacy of 70%. Our in vitro findings highlighted that both pure and encapsulated tannins have efficient inhibition capacities of pancreatic lipase activity. However, the inclusion complex has the greatest, in vivo, antioxidant, and antihyperlipidemic effects. In fact, results showed that complexed tannins had markedly restored serum lipid biomarkers, lipid peroxidation, protein carbonyl oxidation, and antioxidant enzyme defense. These findings were additionally confirmed by aortic and myocardial muscle sections of histological examination. Consequently, ß-cyclodextrin microencapsulation may be considered as an effective and promising technique for tannin delivery with improved antioxidant and antihyperlipidemic activities.
Asunto(s)
Periploca , Proantocianidinas , beta-Ciclodextrinas , Animales , Antioxidantes/metabolismo , Hipolipemiantes/farmacología , Estrés Oxidativo , Periploca/química , Periploca/metabolismo , Proantocianidinas/farmacología , Ratas , Espectroscopía Infrarroja por Transformada de Fourier , Taninos/farmacología , beta-Ciclodextrinas/químicaRESUMEN
Participants in the coastal socio-economy of the Mediterranean Sea, such as industries, aquaculture, urban populations, conglomerates, and tourists, create intense anthropogenic pressures on marine ecosystems (such as the release of trace metals). This raises concerns about their impact on the surrounding environment and on marine organisms, including those collected for human consumption. This study introduces the possibility of using Patella caerulea (Linnaeus 1758), indigenous to the Mediterranean Sea, as a biosentinel of marine pollution. This study proposes coupling environmental (bioaccumulation) and toxicological (redox homeostasis) measures of bioavailability with genetic variability (COI mtDNA) assessments. Concentrations of six trace metals (cadmium, copper, iron, lead, nickel, and zinc) were measured in surface seawater and in P. caerulea individuals collected from four coastal stations on the Tunisian coast where different levels of metal contamination have occurred. The quantified biomarkers involved the determination of antioxidant defense enzymes, catalase (CAT), glutathione peroxidase (GPX), superoxide dismutase (SOD), and the measurement of lipid peroxidation indicated by malondialdehyde (MDA) levels. Our study identified critical levels of metal contamination among locations in the Gulf of Gabes. Concomitantly, the induction of antioxidant biomarkers (especially SOD and GPX) was observed, highlighting the potential of P. caerulea to acclimate to stressful pollution conditions. Molecular analysis of COI (mtDNA) revealed low discrimination between the four P. caerulea populations, highlighting the role of marine currents in the Mediterranean Sea in the dispersal and passive transportation of limpet larvae, allowing an exchange of individuals among physically separated, P. caerulea populations.
Asunto(s)
Gastrópodos , Metales Pesados , Oligoelementos , Contaminantes Químicos del Agua , Animales , Antioxidantes/análisis , Biomarcadores , ADN Mitocondrial , Ecosistema , Monitoreo del Ambiente , Metales Pesados/análisis , Superóxido Dismutasa , Oligoelementos/análisis , Contaminantes Químicos del Agua/análisisRESUMEN
In this research work, a water-soluble polysaccharide (LAP) isolated from the fruits of Lycium arabicum was investigated. LAP contains carbohydrates (82.45±1.23 %), protein (1.56±0.21 %), and uronic acids (3.56±0.34 %). The analysis of the monosaccharide composition revealed the presence of rhamnose, arabinose, galactose, glucose and mannose in a molar ratio of 4.7 : 1.5 : 1 : 8.7 : 16.4 : 5.6. The extracted polysaccharide (PS) was considered as heterogeneous and highly branched by interpreting its GC/MS, FT-IR and NMR data. Crystallinity of LAP was inferred from its X-ray diffractometry (XRD) and Scanning Electron Microscopy (SEM) analysis. LAP exhibited an interesting stability at high temperatures (â¼254 °C) and in a wide range of pH (3-9) deduced, respectively, from its DSC and zeta potential analysis. LAP displayed a strong antioxidant activity at low concentrations evaluated by the 2,2-diphenyl-1-picrylhydrazyl (DPPH)-radical scavenging, ferric reducing activity power (FRAP), free radical scavenging ability, superoxide radical-scavenging and hydroxyl radical-scavenging abilities. Inhibition of erythrocyte hemolysis and lipid peroxidation was also assessed. In 5â h, LAP treatment allowed the protection of the damaged erythrocytes caused by AAPH (2,2-azobis(2-amidinopropane) dihydrochloride), to reduce the level of malondialdehyde (MDA) as well as to increase the reduced glutathione (GSH) level.
Asunto(s)
Antioxidantes/farmacología , Eritrocitos/efectos de los fármacos , Lycium/química , Estrés Oxidativo/efectos de los fármacos , Polisacáridos/farmacología , Amidinas/toxicidad , Antioxidantes/química , Rastreo Diferencial de Calorimetría , Cristalografía por Rayos X , Eritrocitos/metabolismo , Glutatión/sangre , Humanos , Malondialdehído/sangre , Microscopía Electrónica de Rastreo , Polisacáridos/química , Análisis Espectral/métodosRESUMEN
The natural flavonoid (catechin) has been shown to possess a multitude of pharmacological activities. However, oral administrated catechin (CT) failed to fulfil its therapeutic potential due to poor absorption and low bioavailability. Thus, is a pressing need to develop a new approach from to increase its intestinal absorption and improved bioavailability. In this work, we intended the increase the bioavailability of CT by preparing catechin-phospholipid complex (CT-PH) and evaluate the protective effect of CT-PH complex against cadmium caused liver injuries in rats. Oral bioavailability of CT and CT-PH complex was evaluated in rats and the plasma CT was estimated by HPLC analysis. The greater absorption of CT-PH complex rats indicated that improved bioavailability. Liver function markers, lipid peroxidation, protein oxidation, antioxidant status and histopathological changes were determined in normal and treated rats. Moreover, biochemical analysis and histopathological examinations indicated that CT-PH provided better protection to rat liver than free CT.
Asunto(s)
Antioxidantes/farmacología , Catequina/farmacología , Enfermedad Hepática Inducida por Sustancias y Drogas/prevención & control , Hígado/efectos de los fármacos , Estrés Oxidativo/efectos de los fármacos , Fosfatidilcolinas/farmacocinética , Administración Oral , Animales , Antioxidantes/química , Antioxidantes/farmacocinética , Disponibilidad Biológica , Cloruro de Cadmio/toxicidad , Catalasa/metabolismo , Catequina/química , Catequina/farmacocinética , Enfermedad Hepática Inducida por Sustancias y Drogas/metabolismo , Enfermedad Hepática Inducida por Sustancias y Drogas/patología , Esquema de Medicación , Portadores de Fármacos , Expresión Génica/efectos de los fármacos , Glutatión/metabolismo , Glutatión Peroxidasa/metabolismo , Peroxidación de Lípido/efectos de los fármacos , Hígado/metabolismo , Hígado/patología , Pruebas de Función Hepática , Masculino , Malondialdehído/metabolismo , Fosfatidilcolinas/química , Ratas , Ratas Wistar , Superóxido Dismutasa/metabolismoRESUMEN
The present study was performed to assess the HPLC-DAD analysis as well as antioxidant and protective effects of Tunisian Rhanterium suaveolens (Rs) against acetamiprid (ACT) induced oxidative stress on mice erythrocytes. The inâ vitro assays showed that the methanolic extract of Rs has an impressive antioxidant effect proved by testing the total antioxidant and scavenging activities using BCB, DPPH and ABTS assays, respectively. Moreover, qualitative and quantitative analysis using HPLC-DAD revealed the richness of Rs in polyphenols where p-Coumaric, Apigenin-7-glucoside and Ferulic acid were detected as the most abundant polyphenols. In the inâ vivo experiment, ACT, used as a toxicity model, was given to mice at a dose of 20â mg/kg. The latter was the origin of hemolytic anemia characterized by a significant decrease in red blood cells, hemoglobin and hematocrit levels and an increase in bilirubin, LDH, osmotic fragility, reticulocytes and white blood cells number. Characteristic erythrocyte morphological alterations were also determined as spherocytosis, schistocytosis and dacryocystitis. The oxidative status of ACT-treated mice was also altered manifested by a significant increase in MDA and GSH levels and a decrease in SOD, CAT and GPx activities. When receiving the Rs methanolic extract at a dose of 300â mg/kg, all the parameters cited above were restored in mice. These remarkable corrections could only confirm the important antioxidant effect and the noticeable protective properties that possess Rs owing to its broad range of secondary bioactive metabolites.
Asunto(s)
Antioxidantes/análisis , Asteraceae/química , Cromatografía Líquida de Alta Presión/métodos , Neonicotinoides/farmacología , Estrés Oxidativo/efectos de los fármacos , Sustancias Protectoras/análisis , Animales , Antioxidantes/química , Antioxidantes/farmacología , Asteraceae/metabolismo , Eritrocitos/citología , Eritrocitos/efectos de los fármacos , Eritrocitos/metabolismo , Flavonoides/química , Flavonoides/farmacología , Ratones , Extractos Vegetales/química , Polifenoles/química , Polifenoles/farmacología , Sustancias Protectoras/química , Sustancias Protectoras/farmacología , Superóxido Dismutasa/metabolismo , TúnezRESUMEN
In our study, we focused on the optimization; antioxidant and hepatoprotective potentials of novel pigment-protein complex(C-PC) isolated from Phormidium versicolor against cadmium induced liver injury in rats. From analysis, the C-PC extraction parameters were optimized using the response surface methodology (RSM) for optimal recoveries of C-PC extraction. For analysis, the optimum operational conditions for maximizing phycocyanins concentration (67.45mg/g DM) were found to be water/solid 2, temperature 32.5°C and pH7.2.This pigment was identified using HPLC and FTIR analysis. In addition, the molecular masses of α and ß subunits were 17 and 19kDa. Scavenging activity of superoxide anion, hydroxyl, nitric oxide radicals and metal chelating in vitro results indicated that C-PC has an excellent capacity as antioxidant. In vivo study, C-PC significantly prevented cadmium-induced elevation of ALAT, ASAT and bilirubin levels in rats. The histopathological observations supported the results serum enzymes assays. The results of this study revealed that C-PC has significant hepatoprotective potential. C-PC (50mgkg-1 body weight) significantly enhanced the levels of antioxidant enzymes. It can be concluded that C-PC possesses prevention action against hepatotoxicity caused by cadmium.
Asunto(s)
Biotecnología/métodos , Citoprotección/efectos de los fármacos , Hígado/efectos de los fármacos , Microalgas/metabolismo , Ficocianina/biosíntesis , Ficocianina/farmacología , Animales , Antioxidantes/aislamiento & purificación , Antioxidantes/metabolismo , Antioxidantes/farmacología , Cadmio/toxicidad , Glicosilación/efectos de los fármacos , Concentración de Iones de Hidrógeno , Hígado/citología , Hígado/lesiones , Hígado/metabolismo , Ficocianina/aislamiento & purificación , Ratas , TemperaturaRESUMEN
The roots of Stipa species have been used for treatment and prevention of a various number of diseases. To the best of my knowledge, little information is regarding the antioxidant and hepatoprotective activities of polysaccharides from Stipa parviflora (SPP). Hence the polysaccharides from this plant sample have been investigated here. In addition, Box-Behnken design and response surface methodology were used to optimize the hot extraction conditions. The optimal conditions were determined as: extraction time 120â¯min, extraction temperature 70⯰C and ratio of water to raw material 30. Crude SPP was composed of mannose, ribose, glucose, galactose, pyranose and arabinose. In addition, the SPP showed a strong antioxidant capacity in vitro. In vivo experiments showed that CCl4 treatment caused significant oxidative damage in hepatic tissues, whereas these problems were inhibited by SPP treatment. Hepatoprotective potential of SPP was evaluated by estimating the levels of serum markers like ALT and AST levels. SPP treated rat liver anti-oxidant parameters (SOD, CAT and GPx) were significantly antagonized for the pro-oxidant effect of CCl4. Histopathological studies proved that the SPP extract has significant hepatoprotective activity. In conclusion, the SPP fraction isolated from S. parviflora showed significant hepatoprotective potential which could be related to active compounds present in this fraction.
Asunto(s)
Antioxidantes/farmacología , Tetracloruro de Carbono/efectos adversos , Enfermedad Hepática Inducida por Sustancias y Drogas/prevención & control , Citoprotección/efectos de los fármacos , Hígado/efectos de los fármacos , Poaceae/química , Polisacáridos/farmacología , Animales , Antioxidantes/química , Benzotiazoles/química , Compuestos de Bifenilo/química , Enfermedad Hepática Inducida por Sustancias y Drogas/metabolismo , Enfermedad Hepática Inducida por Sustancias y Drogas/patología , Hígado/metabolismo , Hígado/patología , Masculino , Malondialdehído/metabolismo , Estrés Oxidativo/efectos de los fármacos , Picratos/química , Polisacáridos/química , Carbonilación Proteica/efectos de los fármacos , Ratas , Ratas Wistar , Ácidos Sulfónicos/químicaRESUMEN
Periploca angustifolia has numerous biological properties and also used against various diseases. In our study, this plant has been used for biotechnological production of polysaccharides. The prevention action of polysaccharides isolated from P. angustifolia (PAPS) against Cd-caused oxidative stress in HEK293 cells and kidneys of rats was tested. PAPS were characterized by HPLC, FT-IT, DRX, 1D-and 2D-MNR. PAPS present strong capacity to slow the rate of lipid peroxidation and protein glycation in vitro. In addition, the results indicated that the Cd treatment caused a significant decrease in HEK293 cells viability which wasattenuated by PAPS pre-treatment. Furthermore, our findings revealed that Cd injection increased the levels of urea and creatinine in the serum. The increased levels of protein oxidation and lipid peroxidation along with decreased activities of SOD, CAT and GPx were significantly (pâ¯<â¯0.01) ameliorated by PAPS pre-treatment. Finally, histopathological studies also supported the prevention action of PAPS.
Asunto(s)
Antioxidantes/farmacología , Cadmio/farmacología , Riñón/efectos de los fármacos , Periploca/química , Polisacáridos/farmacología , Sustancias Protectoras/farmacología , Animales , Antioxidantes/química , Línea Celular , Creatinina/metabolismo , Células HEK293 , Humanos , Peroxidación de Lípido/efectos de los fármacos , Estrés Oxidativo/efectos de los fármacos , Polisacáridos/química , Ratas , Ratas Wistar , Urea/metabolismoRESUMEN
The nano-encapsulation of Periploca angustifolia phenolic extract using the macrocyclic carbohydrate polymers (ßcyclodextrins) is a most approach compared with other encapsulation methods. In this work, the ßCyclodextrins-PAE complex stability has been evaluated by advanced analytical methods and techniques including HPLC, FTIR and XRD. The results showed that CdCl2 treatment caused a significant decrease in cell viability. The CdCl2-induced damage in the HepG2 cells were significantly ameliorated (pâ¯<â¯0.001) by treatment of the PAE and ßCyclodextrins-PAE complex. Thus, pretreatment with 100⯵gâ¯mL-1 of ßCyclodextrins-PAE complex significantly protect HepG2 cells against cytotoxicity induced by cadmium exposure more effectively than PAE only. However, Cd-intoxication significantly (pâ¯<â¯0.001) increased these enzymes activity. Additionally, reactive oxygen species generation was significantly decreased when cells were treated with nano-encapsulation PAE. The levels of supernatant antioxidant parameters including superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) and GSH were significantly (pâ¯<â¯0.001) decreased in Cd-treated cells with concomitant enhancement of lipid peroxidation. In addition, ßCyclodextrins-PAE pretreatment significantly (pâ¯<â¯0.01) inhibited Cd-exposure activated the apoptotic pathway caspace-3 and caspace-9. This effect may be due to the ability of ßCyclodextrins molecules to enhance stability and permeability properties.
Asunto(s)
Cadmio/farmacología , Compuestos Macrocíclicos/química , Nanopartículas/química , Periploca/química , Extractos Vegetales/química , Sustancias Protectoras/química , beta-Ciclodextrinas/química , Antioxidantes/metabolismo , Catalasa/metabolismo , Línea Celular Tumoral , Enfermedad Hepática Inducida por Sustancias y Drogas/tratamiento farmacológico , Enfermedad Hepática Inducida por Sustancias y Drogas/metabolismo , Glutatión/metabolismo , Glutatión Peroxidasa/metabolismo , Células Hep G2 , Humanos , Peroxidación de Lípido/efectos de los fármacos , Hígado/efectos de los fármacos , Hígado/metabolismo , Estrés Oxidativo/efectos de los fármacos , Extractos Vegetales/farmacología , Polímeros/química , Sustancias Protectoras/farmacología , Superóxido Dismutasa/metabolismoRESUMEN
These studies were designed to evaluate the preliminary oral toxicity profile of the crude ethanolic aerial part extract of E. bivonae in the Male albino Wistar rats and its active chemical constituents. The 24-h LD50 was determined using probit analysis method. The single dose LD50was found to be 2568.64 mg/kg bw when administrated orally in mice. Additionally, the Wistar rats were used to evaluate the subchronic toxicity of E. bivonae ethanolic extract. The serum biomarkers, lipid peroxidation and antioxidants status in liver and histopathological analysis were investigated in normal and treated groups. Subchronic toxicity studies in rats with oral doses of 50, 150, 350 and 500 mg/kg body weight showed significant increase in alanine aminotransferase, aspartate aminotransferase and total bilirubin levels. In addition, the administration of this extract significantly (p < 0.05) decreased superoxide dismutase, catalase and glutathione peroxidase and an increment in lipid peroxidation and protein carbonyls. Finally, we suggest that the three compounds of E. bivonae extract (sitosterol, euphol and lupeol) are the mainly responsible of this toxicity.
RESUMEN
For the first time, we have determined the effect of solvent, liquid-solid ratio and extraction time on polysaccharides yield was evaluated using a full factorial design (23). In this present investigation, a total of 7 molecules were determined in this species. In our analysis saccharose was the dominant monosaccharides. Arabinose, pyranose, fructose, glucose, inositol, saccharose and trehalose found in E. bivonae. The results of the in vitro antioxidant assay showed that the EBPS have higher antioxidant capacity. Accordingly, the HEK293 cells pre-treated with EBPS compounds (100⯵g·mL-1) enhanced cell viability against H2O2 exposure. Our results revealed that H2O2-exposure induced a significant increase in intracellular ROS and lipid peroxidation in HEK293 cells. Additionally, the H2O2-induced alteration in HEK293 cells morphology, was ameliorated by EBPS treatment. In addition, EPBS pre-treated cells significantly enhanced the activities of HEK293 cells antioxidant status (SOD, CAT, GPx and GSH) that were decreased after hydrogen peroxide exposure.
Asunto(s)
Antioxidantes/farmacología , Apoptosis/efectos de los fármacos , Euphorbia/química , Polisacáridos/farmacología , Línea Celular , Supervivencia Celular/efectos de los fármacos , Células HEK293 , Humanos , Peróxido de Hidrógeno/farmacología , Peroxidación de Lípido/efectos de los fármacos , Estrés Oxidativo/efectos de los fármacos , Especies Reactivas de Oxígeno/metabolismo , Superóxido Dismutasa/metabolismoRESUMEN
The in vitro antioxidant, cytotoxic and cytoprotective properties and in vivo hepatoprotective activities of crude polysaccharides extracted from cyanobacteria Phormidim versicolor NCC466 (CFv-PS) were investigated. The CFv-PS, identified as heteropolysaccharides with molecular weight of 63.79kDa, exhibited relatively strong antioxidant activity, in a concentration-depended manner, in vitro assays. Additionally, CFv-PS did not induce cytotoxic effect on HepG2 human hepatocellular carcinoma cells within the range of tested concentrations (25-150µg·mL-1) while preventing them against Cd. Moreover, in rats subjected to Cd-induced hepatotoxicity, CFv-PS pretreatment significantly (P<0.05) reduced the level of ALAT, ASAT, biliburin, MDA, protein carbonyl and DNA damage, and markedly increased enzyme activities in liver tissues. These findings suggest that the cyanobacteria Phormidium versicolor is a potential source of natural products possessing antioxidant, cytoprotective and hepatoprotective properties.
Asunto(s)
Cadmio/toxicidad , Carcinoma Hepatocelular/patología , Cianobacterias/química , Citoprotección/efectos de los fármacos , Neoplasias Hepáticas/patología , Hígado/citología , Polisacáridos Bacterianos/farmacología , Animales , Antioxidantes/farmacología , Relación Dosis-Respuesta a Droga , Células Hep G2 , Humanos , Hígado/efectos de los fármacos , Hígado/metabolismo , Masculino , Estrés Oxidativo/efectos de los fármacos , Ratas , Ratas WistarRESUMEN
BACKGROUND: Mentha piperita L. is a flowering plant belonging to the Lamiaceae family. Mentha plants constitute one of the main valuable sources of essential oil used in foods and for medicinal purposes. METHODS: The present study aimed to investigate the composition and in vitro antioxidant activity of Mentha piperita leaf essential oil (MpEO). A single dose of CCl4 was used to induce oxidative stress in rats, which was demonstrated by a significant rise of serum enzyme markers. MpEO was administrated for 7 consecutive days (5, 15, 40 mg/kg body weight) to Wistar rats prior to CCl4 treatment and the effects on serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), lactate dehydrogenase (LDH), and γ -glutamyl transpeptidase (γ-GT) levels, as well as the liver and kidney superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx) activity and thiobarbituric acid reactive substances (TBARS) levels were evaluated. In addition, histopathological examinations of livers and kidneys was performed. RESULTS: The in vitro antioxidant activity of MpEO was lower than that of silymarin. Pretreatment of animals with MpEO at a dose of 5 mg/kg did not have a significant effect on ALT, AST, ALP, LDH, γGT, urea or creatinine levels in CCl4-induced stress. Whereas pretreatment with MpEO at doses of 15 and 40 mg/kg prior to CCl4, significantly reduced stress parameters (ALT, AST, ALP, LDH, γGT, urea and creatinine) compared to the CCl4-only group. Moreover, a significant reduction in hepatic and kidney lipid peroxidation (TBARS) and an increase in antioxidant enzymes SOD, CAT and GPx was also observed after treatment with MpEO (40 mg/kg) compared to CCl4-treated rats. Furthermore, pretreatment with MpEO at 40 mg/kg can also markedly ameliorate the histopathological hepatic and kidney lesions induced by administration of CCl4. CONCLUSIONS: We could demonstrate with this study that MpEO protects liver and kidney from CCl4-induced oxidative stress and thus substantiate the beneficial effects attributed traditionally to this plant.
Asunto(s)
Antiinflamatorios/farmacología , Antioxidantes/farmacología , Tetracloruro de Carbono/toxicidad , Enfermedad Hepática Inducida por Sustancias y Drogas/prevención & control , Mentha piperita/química , Aceites Volátiles/farmacología , Insuficiencia Renal/prevención & control , Alanina Transaminasa/sangre , Fosfatasa Alcalina/sangre , Animales , Antiinflamatorios/aislamiento & purificación , Antioxidantes/aislamiento & purificación , Aspartato Aminotransferasas/sangre , Catalasa/sangre , Enfermedad Hepática Inducida por Sustancias y Drogas/etiología , Enfermedad Hepática Inducida por Sustancias y Drogas/metabolismo , Enfermedad Hepática Inducida por Sustancias y Drogas/patología , Esquema de Medicación , Glutatión Peroxidasa/metabolismo , Riñón , L-Lactato Deshidrogenasa/sangre , Hígado , Masculino , Aceites Volátiles/aislamiento & purificación , Estrés Oxidativo , Extractos Vegetales/química , Ratas , Ratas Wistar , Insuficiencia Renal/inducido químicamente , Insuficiencia Renal/metabolismo , Insuficiencia Renal/patología , Superóxido Dismutasa/metabolismo , Sustancias Reactivas al Ácido Tiobarbitúrico/metabolismo , gamma-Glutamiltransferasa/sangreRESUMEN
A total of five components (Catechin, Caffeic acid, Ferulic acid, Rosmarinic acid, and Amentoflavone) were identified in Periploca angustifolia leaf methanolic extract. This extract did not cause any cytotoxic effect on HepG2 cell line within the range of concentrations tested (0-400 µg mL-1). Thus, pre-treatment with 100 µg mL-1 of P. angustifolia leaf methanolic extract (PAE) significantly (p < .05) protective HepG2 cells against cytotoxicity induced by cadmium exposure. However, Cd-intoxication significantly (p < .05) increased alanine and aspartate amino transferases serum activities (ALT and AST) and bilirubin content by 1.85-, 1.13-, and 3.55-fold, respectively. The levels of hepatic antioxidant parameters including superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx) were significantly (p < .05) decreased in Cd-intoxicated rats with concomitant enhancement of lipid peroxidation. Our results showed that P. angustifolia leaf methanolic extract can induce antioxidant effects and also exerts beneficial effects for the treatment of Cd-induced hepatotoxicity.
Asunto(s)
Antioxidantes/farmacología , Cadmio/toxicidad , Hígado/efectos de los fármacos , Hígado/metabolismo , Estrés Oxidativo/efectos de los fármacos , Periploca/química , Fenoles/farmacología , Animales , Antioxidantes/química , Apoptosis/efectos de los fármacos , Supervivencia Celular/efectos de los fármacos , Citoprotección/efectos de los fármacos , Ácidos Grasos/metabolismo , Células Hep G2 , Humanos , Hígado/citología , Ratones , Fenoles/química , RatasRESUMEN
The extraction of Periploca polysaccharides (PAPS) was optimized using the response surface methodology. The influence of solvent, liquid-solid ratio and extraction time on polysaccharide yield was evaluated using a full factorial design (23). Also, PAPS extract did not induce a cytotoxic effect on HepG2 cells within the range of tested concentrations (0-250µgmL-1). Herein, the pre-treatment with PAPS extract (100µgmL-1) reduced cell mortality. Furthermore, the in vivo antioxidant activity of PAPS extract was investigated in rats. The oral administration of 250mgkg-1 body weight of PAPS extract administered above a period of 10 weeks to cadmium chloride (CdCl2) induced toxicity in male Wistar rats, markedly decreased the content of MDA and protein damage in liver tissue, and enhanced liver function parameters (ALAT, ASAT and bilirubin), as well as the activities of hepatic antioxidant status (SOD, CAT, GPx and GSH). Finally, the examination of liver histopathology confirmed that PAPS ameliorate the alteration of liver tissue caused by exposition to cadmium.
Asunto(s)
Antioxidantes/farmacología , Quelantes/farmacología , Periploca/química , Extractos Vegetales/farmacología , Polisacáridos/farmacología , Animales , Antioxidantes/química , Cadmio/efectos adversos , Cloruro de Cadmio/efectos adversos , Supervivencia Celular/efectos de los fármacos , Células Cultivadas , Quelantes/química , Fraccionamiento Químico , Enfermedad Hepática Inducida por Sustancias y Drogas/tratamiento farmacológico , Enfermedad Hepática Inducida por Sustancias y Drogas/metabolismo , Enfermedad Hepática Inducida por Sustancias y Drogas/patología , Modelos Animales de Enfermedad , Relación Dosis-Respuesta a Droga , Cromatografía de Gases y Espectrometría de Masas , Células Hep G2 , Humanos , Masculino , Estrés Oxidativo/efectos de los fármacos , Extractos Vegetales/química , Polisacáridos/química , RatasRESUMEN
Amphora sp. was isolated from the Sfax Solar Saltern and cultivated under hypersaline conditions. It contains moderate rates of proteins, lipids, sugars, and minerals and a prominent content of bioactive compounds: polyphenols, chlorophyll a, carotenoids, and fatty acids. The analysis of fatty acids with GC/MS showed that the C16 series accounted for about 75% of Amphora sp. lipids. Saturated fatty acids whose palmitic acid was the most important (27.41%) represented 41.31%. Amphora sp. was found to be rich in monounsaturated fatty acids with dominance of palmitoleic acid. It also contains a significant percentage of polyunsaturated fatty acids with a high amount of eicosapentaenoic acid (2.36%). Among the various solvents used, ethanol at 80% extracted the highest amounts of phenols and flavonoids that were 38.27 mg gallic acid equivalent and 17.69 mg catechin equivalent g-1 of dried extract, respectively. Using various in vitro assays including DPPH and ABTS radicals methods, reducing power assay, and ß-carotene bleaching assay, the 80% ethanolic extract showed high antioxidant activity. A strong antibacterial activity was checked against Gram-positive bacteria (Staphylococcus aureus and Micrococcus luteus) and Gram-negative bacteria (Klebsiella pneumoniae and Salmonella enterica). These results are in favor of Amphora sp. valorization in aquaculture and food and pharmaceutical industries.
Asunto(s)
Medios de Cultivo/química , Ácido Eicosapentaenoico/biosíntesis , Ácidos Grasos Monoinsaturados/metabolismo , Microalgas/crecimiento & desarrollo , Ácido Palmítico/metabolismo , Phaeophyceae/crecimiento & desarrolloRESUMEN
In this study, response surface methodology (RSM) based on Box-Behnken design (BBD) was employed to optimize the aqueous extraction of crude polysaccharides from Tunisian cyanobacteria Phormidium versicolor (NCC 466). The optimal extraction conditions with an extraction yield of 21.56±0.92% were as follows: extraction temperature at 81.05°C, extraction time of 3.99h, and water to raw material ratio of 21.52mLg-1. Crude Phormidium versicolor polysaccharides (CPv-PS) are found to be a hetero-sulfated-anionic polysaccharides that contained carbohydrate (79.37±1.58%), protein (0.45±0.11%), uronic acids (4.37±0.19%) and sulfate (6.83±0.28%). The carbohydrate fraction was composed of arabinose, xylose, ribose, rhamnose, N-acetyl glucosamine, galactose, glucose, mannose, glucuronic acid and saccharose with corresponding mole percentages of 2.41, 14.58, 2.18, 6.23, 7.04, 28.21, 26.04, 3.02, 0.86 and 5.07, respectively. Evaluation of the antioxidant activity in vitro suggested that CPv-PS strongly scavenged radicals, prevented bleaching of ß-carotene and reduced activity. Furthermore, the CPv-PS exhibited effective antimicrobial properties.
Asunto(s)
Fraccionamiento Químico/métodos , Cianobacterias/química , Polisacáridos Bacterianos/aislamiento & purificación , Polisacáridos Bacterianos/farmacología , Antiinfecciosos/química , Antiinfecciosos/aislamiento & purificación , Antiinfecciosos/farmacología , Antioxidantes/química , Antioxidantes/aislamiento & purificación , Antioxidantes/farmacología , Compuestos de Bifenilo/química , Radical Hidroxilo/química , Hierro/química , Oxidación-Reducción/efectos de los fármacos , Picratos/química , Polisacáridos Bacterianos/química , Temperatura , Agua/química , beta Caroteno/químicaRESUMEN
Objective. Artichoke (Cynara scolymus L.) was one of the plant remedies for primary health care. The present study was focused on the determination of chemical composition, antioxidant activities, and anti-inflammatory activity and on analyzing its major bioactive polyphenols by HPLC. Methods. Artichoke Leaves Extracts (ALE) were analyzed for proximate analysis and phytochemical and antioxidant activity by several methods such as DDPH, ABTS, FRAP, and beta-carotene bleaching test. The carrageenan (Carr) model induced paw oedema in order to investigate the anti-inflammatory activity. Identification and quantification of bioactive polyphenols compounds were done by HPLC method. The oxidative stress parameters were determined; CAT, SOD, GSH, MDA, and AOPP activities and the histopathological examination were also performed. Results. It was noted that EtOH extract of ALE contained the highest phenolic, flavonoid, and tannin contents and the strongest antioxidants activities including DDPH (94.23%), ABTS (538.75 mmol), FRAP assay (542.62 umol), and ß-carotene bleaching (70.74%) compared to the other extracts of ALE. Administration of EtOH extract at dose 400 mg/kg/bw exhibited a maximum inhibition of inflammation induced by Carr for 3 and 5 hours compared to reference group Indomethacin (Indo). Conclusion. ALE displayed high potential as natural source of minerals and phytochemicals compounds with antioxidant and anti-inflammatory properties.