RESUMEN
After rifampin and levofloxacin treatment for a Staphylococcus aureus bone infection, a pyogenic granuloma due to a newly described Cutibacterium species, C. namnetense developed on the tibia former external fixator. This rifampin resistant bacterium, selected during treatment, harbored a mutation in the rpoB gene. This case illustrates the possible in vivo selection of resistant mutant most likely due to the bacterial burden and therefore the importance of adequate bone infection treatment.
RESUMEN
Cutibacterium (formerly Propionibacterium) acnes is involved in chronic/low-grade pathologies such as sarcoidosis or prosthetic joint infection (PJI). In these diseases, granulomatous structures are frequently observed. In this study, we induced a physiological granulomatous reaction in response to different well-characterized clinical C. acnes isolates in order to investigate the cellular process during granuloma formation. Three C. acnes isolates selected according to their origin (PJI, sarcoidosis and acne) were typed by MLST. All C. acnes isolates generated granulomatous structures in our experimental conditions. The bacterial burden was better controlled by granulomas induced by the sarcoidosis C. acnes isolate. The PJI C. acnes isolate, belonging to CC36, promoted the recruitment of CD8+ lymphocytes inside the granuloma. In contrast, the acne and sarcoidosis C. acnes isolates, belonging to phylotypes IA1/CC18 and IA2/CC28, respectively, generated a higher number of granulomas and promoted the recruitment of CD4+ lymphocytes inside the granuloma. Our results provide new evidence supporting the role of C. acnes in the development of sarcoidosis and new explanations concerning the mechanisms underlying PJI due to C. acnes.
Asunto(s)
Infecciones por Bacterias Grampositivas/complicaciones , Infecciones por Bacterias Grampositivas/inmunología , Granuloma/etiología , Inmunidad , Propionibacterium acnes/inmunología , Susceptibilidad a Enfermedades , Humanos , Leucocitos Mononucleares/inmunología , Leucocitos Mononucleares/microbiología , Tipificación de Secuencias Multilocus , Propionibacterium acnes/clasificación , Propionibacterium acnes/genéticaRESUMEN
The recognition of the pathogenicity of Cutibacterium acnes in implant-associated infection is not always obvious. In this paper, we aimed to distinguish pathogenic and non-pathogenic C. acnes isolates. To reach this goal, we investigated the clonal complex (CC) of a large collection of C. acnes clinical isolates through Multi-Locus Sequence Typing (MLST), we established a Caenorhabditis elegans model to assess C. acnes virulence and we investigated the presence of virulence factors in our collection. Ours results showed that CC36 and CC53 C. acnes isolates were more frequently observed in prosthetic joint infections (PJI) than CC18 and CC28 C. acnes isolates (p = 0.021). The C. elegans model developed here showed two distinct virulence groups of C. acnes (p < 0.05). These groups were not correlated to CC or clinical origin. Whole genome sequencing allowed us to identify a putative gene linked to low virulent strains. In conclusion, MLST remains a good method to screen pathogenic C. acnes isolates according to their clinical context but mechanisms of C. acnes virulence need to be assess thought transcriptomic analysis to investigate regulatory process.
Asunto(s)
Infecciones por Bacterias Grampositivas/microbiología , Propionibacterium acnes/fisiología , Propionibacterium acnes/patogenicidad , Infecciones Relacionadas con Prótesis/microbiología , Tropismo Viral , Animales , Caenorhabditis elegans/microbiología , Caenorhabditis elegans/fisiología , Modelos Animales de Enfermedad , Humanos , Tipificación de Secuencias Multilocus , Propionibacterium acnes/clasificación , Propionibacterium acnes/genética , Análisis de Supervivencia , Virulencia , Factores de Virulencia/análisis , Factores de Virulencia/genética , Secuenciación Completa del GenomaRESUMEN
Cutibacterium acnes (formerly Propionibacterium acnes) is recognized as a pathogen in foreign-body infections (arthroplasty or spinal instrumentation). To date, the direct impact of C. acnes on bone cells has never been explored. The clade of 11 C. acnes clinical isolates was determined by MLST. Human osteoblasts and osteoclasts were infected by live C. acnes. The whole genome sequence of six isolates of this collection was analyzed. CC36 C. acnes strains were significantly less internalized by osteoblasts and osteoclasts than CC18 and CC28 C. acnes strains (p ≤ 0.05). The CC18 C. acnes ATCC6919 isolate could survive intracellularly for at least 96 hours. C. acnes significantly decreased the resorption ability of osteoclasts with a major impact by the CC36 strain (p ≤ 0.05). Genome analysis revealed 27 genes possibly linked to these phenotypic behaviors. We showed a direct impact of C. acnes on bone cells, providing new explanations about the development of C. acnes foreign-body infections.
Asunto(s)
Propionibacterium acnes/fisiología , Enfermedades Óseas/microbiología , Enfermedades Óseas/patología , Línea Celular Tumoral , Supervivencia Celular , Infecciones por Bacterias Grampositivas/microbiología , Infecciones por Bacterias Grampositivas/patología , Humanos , Artropatías/microbiología , Artropatías/patología , Monocitos/citología , Monocitos/inmunología , Monocitos/metabolismo , Tipificación de Secuencias Multilocus , Propionibacterium acnes/genética , Propionibacterium acnes/aislamiento & purificación , Secuenciación Completa del GenomaRESUMEN
Propionibacterium acnes is now well-known and recognized for its implication in the pathogenesis of acne vulgaris. Here, we report the draft genome sequence of an erythromycin-resistant P. acnes strain isolated from a case of folliculitis of the scalp belonging to phylotype IA1 and sequence type 18 (ST18).
RESUMEN
Propionibacterium acnes was previously described as a potential implant-related pathogen. Here, we report the draft genome sequence of four P. acnes strains, isolated from spine material, hip arthroplasty, and knee arthroplasty infections in France belonging to different sequence types (ST18, ST27, and ST36).
RESUMEN
Propionibacterium namnetense was recently described as a potential bone pathogen, which is closely related to Propionibacterium acnes, a skin commensal microorganism. Here, we report the draft genome sequence of the highly rifampin-resistant strain NTS 31307302(T) isolated from a patient with a tibia infection.
RESUMEN
A polyphasic taxonomic study was performed on two Gram-positive-staining, anaerobic, pleomorphic, rod-shaped strains isolated from human bone and tissue samples. Sequencing of the 16S rRNA genes revealed that the strains belong to a novel species within the genus Propionibacterium, most closely related to Propionibacterium acnes subsp. acnes and Propionibacterium acnes subsp. elongatum with similarity values of 98.4 % and 98.1 %, respectively. In addition, protein-coding genes for rpoB, recA and gyrB clearly separated the novel organism from all species and subspecies of the genus Propionibacterium. However, a DNA-DNA hybridization analysis between the novel organism and the type strain P. acnes ATCC 6919T revealed a value of only 61.1 %. Furthermore, whole genome analysis using the program OrthoANI gave a value of 88.5 %, which is significantly below the cut-off value of 95 % for species delineation. The major fatty acids were iso-C15 : 0, anteiso-C15 : 0 and iso-C17 : 0. The DNA G+C content of the type strain was 59.7 mol%. When taken collectively, phenotypic, molecular genetic, chemotaxonomic and phylogenetic information demonstrate that the organism represents a distinct, albeit close relative of P. acnes On the basis of the results presented, the organism represents a novel member of the genus Propionibacterium for which the name Propionibacterium namnetense sp. nov. is proposed. The type strain is NTS 31307302T (=DSM 29427T=CCUG 66358T).
Asunto(s)
Enfermedades Óseas Infecciosas/microbiología , Filogenia , Propionibacterium/clasificación , Técnicas de Tipificación Bacteriana , Composición de Base , ADN Bacteriano/genética , Ácidos Grasos/química , Francia , Humanos , Masculino , Hibridación de Ácido Nucleico , Propionibacterium/genética , Propionibacterium/aislamiento & purificación , ARN Ribosómico 16S/genética , Análisis de Secuencia de ADN , Adulto JovenAsunto(s)
Antibacterianos/farmacología , Escherichia coli/efectos de los fármacos , Fluoroquinolonas/farmacología , Resistencia a la Tetraciclina , Combinación Trimetoprim y Sulfametoxazol/farmacología , Anciano , Anciano de 80 o más Años , Farmacorresistencia Bacteriana Múltiple , Escherichia coli/aislamiento & purificación , Femenino , Humanos , Masculino , Persona de Mediana EdadRESUMEN
AIM: To identify bacterial and/or clinical features involved in the pathogenesis of Staphylococcus aureus implant-associated infections (IAI). MATERIALS & METHODS: In total, 57 IAI S. aureus and 31 nasal carriage (NC) S. aureus isolates were studied. Staphylococcus aureus genetic background was obtained by microarray analysis. Multilocus sequence typing was performed to determine clonal complexes (CC). Biofilm production was investigated by resazurin and crystal violet methods. RESULTS: Staphylokinase gene was associated with the occurrence of S. aureus IAI. Patients' ABO blood group phenotype was associated with IAI S. aureus genetic background. CC8 S. aureus strains produce more biofilm than others and carry particular alleles of bbp gene. CONCLUSION: This study identifies some predictive markers for S. aureus IAI.
Asunto(s)
Sistema del Grupo Sanguíneo ABO/metabolismo , Interacciones Huésped-Patógeno , Metaloendopeptidasas/metabolismo , Infecciones Relacionadas con Prótesis/microbiología , Infecciones Estafilocócicas/microbiología , Staphylococcus aureus/aislamiento & purificación , Adulto , Anciano , Anciano de 80 o más Años , Femenino , Genotipo , Humanos , Masculino , Análisis por Micromatrices , Persona de Mediana Edad , Tipificación de Secuencias Multilocus , Fenotipo , Staphylococcus aureus/enzimología , Staphylococcus aureus/patogenicidad , Adulto JovenRESUMEN
We described for the first time the amino acid substitutions conferring rifampicin resistance in eight Propionibacterium acnes strains isolated from patients with biofilm or device-related infections. We identified different mutations in cluster I and one mutation, never reported, in cluster II of the rpoB gene (I480V) associated with the most frequent one in cluster I (S442L). Half of the patients previously received treatment with rifampicin.
Asunto(s)
Biopelículas , Infecciones Relacionadas con Catéteres/microbiología , ARN Polimerasas Dirigidas por ADN/genética , Farmacorresistencia Bacteriana , Mutación Missense , Propionibacterium acnes/efectos de los fármacos , Rifampin/farmacología , Adulto , Anciano , Anciano de 80 o más Años , Sustitución de Aminoácidos , Antibacterianos/farmacología , Femenino , Infecciones por Bacterias Grampositivas/microbiología , Humanos , Masculino , Persona de Mediana Edad , Datos de Secuencia Molecular , Propionibacterium acnes/aislamiento & purificación , Análisis de Secuencia de ADNRESUMEN
In this study, we developed and compared four protocols to prepare a bacterial pellet from 944 positive blood cultures for direct MALDI-TOF mass spectrometry Vitek® MS analysis. Protocol 4, tested on 200 monomicrobial samples, allowed 83% of bacterial identification. This easy, fast, cheap and accurate method is promising in daily practice, especially to limit broad range antibiotic treatment.
Asunto(s)
Bacterias/aislamiento & purificación , Técnicas de Tipificación Bacteriana/métodos , Sangre/microbiología , Espectrometría de Masas en Tándem/métodos , Bacterias/química , Bacterias/clasificación , Técnicas de Tipificación Bacteriana/economía , Humanos , Espectrometría de Masa por Láser de Matriz Asistida de Ionización Desorción/economía , Espectrometría de Masa por Láser de Matriz Asistida de Ionización Desorción/métodos , Espectrometría de Masas en Tándem/economíaRESUMEN
We describe the first case of bacteremia due to Gallibacterium anatis. The patient, a 26-year-old woman, developed bacteremia and diarrhea. The origin of infection was possibly due to a diet contaminated by G. anatis in this highly immunocompromised patient.
Asunto(s)
Bacteriemia/diagnóstico , Bacteriemia/microbiología , Infecciones por Pasteurellaceae/diagnóstico , Infecciones por Pasteurellaceae/microbiología , Pasteurellaceae/aislamiento & purificación , Adulto , ADN Bacteriano/química , ADN Bacteriano/genética , ADN Ribosómico/química , ADN Ribosómico/genética , Diarrea/diagnóstico , Diarrea/microbiología , Femenino , Enfermedades Transmitidas por los Alimentos/diagnóstico , Enfermedades Transmitidas por los Alimentos/microbiología , Humanos , Huésped Inmunocomprometido , Datos de Secuencia Molecular , Pasteurellaceae/clasificación , Pasteurellaceae/genética , Infecciones por Pasteurellaceae/complicaciones , ARN Ribosómico 16S/genética , Análisis de Secuencia de ADNRESUMEN
A Proteus mirabilis clinical strain (7001324) was isolated from urine sample of a patient hospitalized in a long-term-care facility. PCR and cloning experiments performed with this strain identified a novel TEM-type ß-lactamase (TEM-187) differing by four amino acid substitutions (Leu21Phe, Arg164His, Ala184Val, and Thr265Met) from TEM-1. This characterization provides further evidence for the diversity of extended-spectrum ß-lactamases (ESBL) produced by P. mirabilis and for their potential spread to other Enterobacteriaceae due to a lack of sensitive detection methods used in daily practice.