Esterilidad de sueros purgados. Antes de su uso en urgencias y emergencias extrahospitalarias / Sterility in drained serums; before their use in emergency wards and in non-hospital emergency use

Se detallan las características de esterilidad de los sueros purgados y ubicados dentro de una UVI-móvil, y se determina cuánto tiempo pueden mantener las condiciones óptimas para su uso, mediante un ensayo clínico controlado y aleatorizado, triple ciego, donde las unidades de estudio fueron sueros preparados con sistema de infusión y llave de tres vías. Se recogieron datos durante un periodo de estudio de 12 meses. Se diseñaron seis grupos de intervención en función del tiempo de exposición y su ubicación. Tamaño muestral de 672 unidades. Se realizaron 12 cultivos microbiológicos a cada una, para bacterias y hongos, tanto aerobios como anaerobios. Se contrastó hipótesis con un modelo de regresión logística multivariante para la variable "esterilidad". Se consideró significativo un valor de p < 0,05. Se obtuvieron resultados de 669 sueros (98,82%). Existió colonización en 1,7% de los 8.028 cultivos realizados, encontrando más de 10 UFC/ml sólo en dos casos. Esta colonización correspondió a 103 sueros que se clasificaron como "no estériles". Para la esterilidad, el modelo de regresión logística no mostró asociación significativa con los diferentes grupos de intervención, controlando mediante dos variables posiblemente confusoras. No se encontraron evidencias de que el suero recién purgado fuera diferente, desde el punto de vista de esterilidad, a los expuestos durante 24, 48 o 72 horas pero, a igualdad de grupos, las incidencias de los sueros triplicaban el riesgo de colonización. Se concluyó que podríamos utilizar sueros preparados con antelación, garantizando una esterilidad similar a los recién purgados(AU)

The authors detail the characteristics for the sterility of drained serums placed in a mobile emergency service unit and they determine how much time they can maintain optimum conditions for the use of these serums by means of a controlled, random, triple blind clinical test in which the units studied were serums prepared by an infusion system and a three-way valve. Data were gathered during a 12 month study period. The authors designed six operating groups based on their exposure time and where they were kept. These samples totaled 672 units. The authors carried out 12 microbiological cultural analyses for each unit; these analyses were tested for bacteria and fungi, both aerobic and anaerobic. The authors contrasted their hypothesis with a multivariable logistic regression model used as the variable for sterility. A value of p < 0.05 was considered to be significant. Results were obtained on 669 serums, 98.82% of samples. Colonization was found in 1.7% of the 8028 cultures analyzed; in only 2 cases was there a register greater than 10 UFC/ml. This colonization corresponded to 103 serums which were then classified as non-sterile. For sterility, the logistic regression model did not show any significant association with the various operating groups, which were controlled by means of two possible variable methods. The authors did not find evidence that recently drained serums were different, from the point of view of sterility, than the serums exposed during 24, 48 or 72 hours but, as well as for the groups, the incidences of these serums tripled the risk of colonization. The authors concluded they could use serums prepared previously and these serums sterility were guaranteed to the same degree as recently drained serums(AU)

Physico-chemical stability and sterility of previously prepared saline infusion solutions for use in out-of-hospital emergencies.

OBJECTIVE: The topic of this research was to determine whether out-of-hospital emergency teams could make use of previously prepared saline solutions (SS). The objective was to discover the physical, chemical and sterility characteristics of previously prepared saline infusions stored in ambulances and ascertain how long they remained in optimum condition. METHOD: Randomised clinical trial, triple blind, where study units consisted of saline solutions prepared with an infusion system and a three-way valve. The duration of the study was 12 months. Six intervention groups were designed on the basis of time of exposure and location. Samples consisted of 672 units. Twelve microbiological cultures were made and the pH, density, viscosity and CINa concentration were determined. We compared hypotheses with four models of linear regression for the variables and a model of logistic regression for the variables. A value of P < 0.05 was considered significant. RESULTS: We obtained results from 669 saline solutions (98.82%). Neither multivariant analysis nor ANOVA tests showed any significant association for a power greater than 99% with regard to the physical-chemical characteristics. The model of logistic regression also did not find any significant association for sterility. Colonisation was present in 1.7% of the 8,028 cultures made and more than 5 CFU per millilitre was found in only two cases. CONCLUSION: There is no evidence to suggest that recently prepared saline infusion solutions are any different from a physical-chemical and sterility point of view than those exposed for 24, 48, or 72 h. It was concluded that use can be made of previously prepared saline solutions with a guarantee their stability and sterility.