[Purification and some properties of 1,3-1,4-beta-glucanase from Bacillus subtilis]. / Ochistka i nekotorye svoistva 1,3-1,4-beta-gliukanazy iz Bacillus subtilis.

Using chromatography on cellulose, SE-Sephadex G-50 and gel filtration on acrylex P-60, 1.3 -- 1.4-beta-glucanase from Bac. subtilis, strain 103 was obtained and purified 142-fold. The specific activity of the purified enzyme was 18.5 units per mg of protein. The homogeneity of 1.3 -- 1.4-beta-glucanase was determined by gel filtration on acrylex P-60, polyacrylamide gel electrophoresis, isoelectrofocusing and ultracentrifugation. Using electrophoresis in Na-SDS and gel filtration on acrylex P-60, the molecular weight of the enzyme was found to be equal to 30 000 and 33 000, respectively. The isoelectric point for the enzyme lies at pH 5.4. The enzyme does not contain tryptophane, free SH-groups or carbohydrates.

[Purification and characterization of beta-fructofuranosidase from yeast Saccharomyces cerevisiae]. / Vydelenie i izuchenie svoistv beta-fruktozuranozidazy iz drozhzhei Saccharomyces cerevisiae.

Intracellular invertase was isolated from the yeast Saccharomyces cerevisiae, race XI, and purified by ion-exchange chromatography on DEAE-cellulose and gel-filtration on Sephadex G-200. The effect of pH, temperature, metal ions, thiolic agents, and EDTA on the enzyme activity and stability was investigated. The enzyme was estimated to have a molecular weight of 270 000 and a carbohydrate content of 20--30%. By disc-electrophoresis and isoelectric focusing the highly purified enzyme was found to be heterogenous. Its molecular forms had isoelectric points at 3.0, 4.0, 4.5, and 4.9.

[Denaturation of the alpha-amylase of Bacillus subtilis in an acid medium]. / Denaturatsiia alpha-amilazy Bacillus subtilis v kisloi srede.

By fractionation of purine compounds (sorbtion on the cation exchange resin KU-2 (H+), extraction, precipitation of purine compounds as Ag-complexes) a "purine fraction" of the culture liquid epiphytic bacteria No. 703 isolated from barley overground organs was obtained. The presence of isopentenyl cytokinins was demonstrated by quality reactions of the purine fraction with aromatic amines and phenols as well as by the values of Rf and UV spectrum of individual compounds examined by paper chromatography of the purine fraction.