Targeted killing of Leishmania donovani in vivo and in vitro with amphotericin B attached to functionalized carbon nanotubes.

OBJECTIVES: This study describes the antileishmanial efficacy of the novel drug formulation of amphotericin B (AmB) attached to functionalized carbon nanotubes (f-CNTs) and compares it with AmB. METHODS: f-CNTs were prepared in a two-step chemical carboxylation and amidation process. The AmB was then attached to make f-CNT-AmB and its construction was confirmed by Fourier transform infrared (FTIR) spectroscopy and transmission electron microscopy (TEM). The cytotoxicity of the constructed compound, f-CNT-AmB, was assessed in vitro using the J774A.1 macrophage cell line and in vivo using healthy BALB/c mice. Antileishmanial activity of AmB and f-CNT-AmB was assessed in vitro using a macrophage (J774A.1 cell line) model of Leishmania donovani infection. Antileishmanial activity was assessed in vivo by comparing the parasite load of hamsters treated with a 5 day course of AmB, f-CNTs or f-CNT-AmB initiated at 30 days after infection with L. donovani parasites. RESULTS: The FTIR spectroscopy and TEM data demonstrate the successful attachment of AmB to f-CNTs. The in vitro cytotoxicity of AmB, f-CNTs and f-CNT-AmB was measured by the cytotoxic concentration required to kill 50% of the cells: 0.48±0.06 µg/mL; 7.31±1.16 µg/mL; 0.66±0.17 µg/mL, respectively, in the J774A.1 cell line. The in vivo toxicity assessment of the compounds in BALB/c mice revealed no hepatic or renal toxicity. Against intracellular amastigotes the in vitro antileishmanial efficacy of f-CNT-AmB was significantly higher than that of AmB (IC50 0.00234±0.00075 µg/mL versus 0.03263±0.00123 µg/mL; P≤0.0001). The percentage inhibition of amastigote replication in hamsters treated with f-CNT-AmB was significantly more than that with AmB (89.85%±2.93% versus 68.97%±1.84%; P=0.0004). CONCLUSIONS: The results of these experiments clearly demonstrate that f-CNT-AmB has significantly greater antileishmanial efficacy than AmB and had no significant cytotoxic effects.

Functionalization effects on the electrical properties of multi-walled carbon nanotube-polyacrylamide composites.

Multi-walled carbon nanotubes (MWNTs)-polyacrylamide (PAM) composites have been prepared using as purified, with ball milling and functionalized MWNTs by solution cast technique and characterized through scanning electron microscopy (SEM) and transmission electron microscopy (TEM). A comparative study has been made on the electrical property of these MWNTs-PAM composites with different MWNTs loadings. It has been shown that the ball milling and functionalization of MWNTs improves the dispersion of MWNTs into the polymer matrix. As the MWNTs loading increases from 0 to 40 wt% electrical conductivity of composite film increases by approximately 7 orders of magnitude whereas the electrical conductivity of functionalized composite film increases only approximately 4 orders of magnitude with respect to the pure PAM film. A model based on power law percolation for the electronic behavior of above composite has been developed and shows good agreement with the experimental data.

Formation and microstructural characterization of coaxial carbon cylinders consisting of aligned carbon nanotube stacks.

The macroscopic coaxial carbon cylinders (dia. approximately 0.5 cm with varying lengths approximately 2-5 cm) consisting of aligned carbon nanotube (CNT) stacks have been prepared by spray pyrolysis of benzene-ferrocene solution in argon atmosphere at approximately 850 degrees C-900 degrees C temperature. The coaxial carbon cylinders of CNT stacks have been formed directly inside the quartz tube. We attempted to prepare superimposed multi carbon cylinder configurations, each consisting of ordered and aligned CNTs stacked over each other. For this, we have terminated the spray of precursor after run of about 25 minutes, for a short interval (approximately 5 min), and then the solution was sprayed again over the already deposited hot CNT stack. Gross structural characterization of CNTs was done through X-ray diffraction technique (XRD). Microstructural characterizations of as prepared coaxial carbon cylinders with CNT stacks were done by scanning electron microscopic (SEM) techniques. SEM studies show that the CNTs are well aligned along the periphery of the cross section of coaxial carbon cylinder, each consisting of CNTs of the type described in the above. Comparisons have been made between the present macroscopic coaxial carbon cylinders with CNT stacks studied earlier by several other workers. Plausible explanation for the synthesis of CNT stacks will be put forward.

Synthesis of carbon nanotubes.

Carbon nanotubes play a fundamental role in the rapidly developing field of nanoscience and nanotechnology because of their unique properties and high potential for applications. In this article, the different synthesis methods of carbon nanotubes (both multi-walled and single-walled) are reviewed. From the industrial point of view, the chemical vapor deposition method has shown advantages over laser vaporization and electric arc discharge methods. This article also presents recent work in the controlled synthesis of carbon nanotubes with ordered architectures. Special carbon nanotube configurations, such as nanocoils, nanohorns, bamboo-shaped and carbon cylinder made up from carbon nanotubes are also discussed.

Formation of carbon nanotubes without iron inclusion and their alignment through ferrocene and ferrocene-ethylene pyrolysis.

So far carbon nanotubes grown from the method most common method at present, that is, pyrolysis of ferrocene, invariably contains Fe inclusion. In addition, they are generally grown in random configurations. In the present investigations CNTs without Fe inclusion and in aligned configurations have been prepared by the pyrolysis of ferrocene (C10H10Fe) as well as pyrolysis of ferrocene in the presence of ethylene (C2H4). This has been achieved through optimization of growth parameters, for example, heating rate of ferrocene, pyrolysis temperature, and flow rates of carrier gas argon (Ar) and ethylene (C2H4). The as-synthesized samples have been characterized by transmission and scanning electron microscopic techniques. The optimum results relating to synthesis of carbon nanotubes without Fe inclusion and in aligned configurations have been obtained at 1000 degrees C pyrolysis temperature under flow rates of Ar of approximately 1000 sccm and of C2H4 of approximately 100 sccm. These carbon nanotubes have been found to have an outer diameter between approximately 20 and 60 nm and lengths between approximately 15 and 20 microns.

Synthesis and characterization of lanthanum carbide nanotubes.

Lanthanum carbide nanotubes have been synthesized by d.c. arc evaporation (approximately 20-30 V, approximately 200 Amp) of lanthanum metal (90 wt.%)-loaded graphite rod in a helium atmosphere (665 mbar). To explore the possibility of formation of lanthanum carbide nanotubes, the experiments were carried out with lanthanum metal in different concentrations (i.e., 30, 50, 70, and 90 wt.%) in the graphite rod. The as-synthesized samples were characterized by transmission electron microscopy and X-ray diffractometry. Lanthanum carbide nanotubes (LCNTs) with a diameter of approximately 65 to 95 nm and a length of approximately 0.2 to 1.5 microns were obtained in this study.