Evaluation of gases emission and enzyme dynamics in sheep manure compost occupying with peach shell biochar.

The effect of peach shell biochar (PSB) amendment on sheep manure (SM) composting was investigated. Five different ratios of PSB were applied (0%, 2.5%, 5%, 7.5%, and 10% PSB), and named T1 to T5, and run 50 days of composting experiment. It was found that PSB (especially 7.5% and 10%) could improve the compost environment, regulate the activity of microorganisms and related enzymes, and promote the decomposition of compost. 7.5% and 10% PSB advanced the heap into the thermophilic stage and increased the maximum temperature, while also increasing the germination index by 1.40 and 1.39 times compared to control. Importantly, 10% PSB effectively retained more than 60% of carbon and 55% of nitrogen by inhibiting the excess release of NH3 and greenhouse gases. High proportion PSB amendment increased the activity of dehydrogenase and cellulase, but inhibited protease and urease. The correlation results indicated that PSB changed the key bacterial genus, and there was a stronger association with environmental factors at 7.5% and 10%. Therefore, 7.5% and 10% peach shell biochar can be used as appropriate proportions to improve composting conditions.

Unlocking growth potential: Synergistic potassium fertilization for enhanced yield, nutrient uptake, and energy fractions in Chinese cabbage.

The implementation of integrated potassium management presents a viable approach for augmenting plant growth, yield, and nutrient uptake while enhancing soil nutrient availability. A field experiment was executed during the rabi season of 2020, employing a randomized complete block design encompassing eight treatments involving standard (100%) and reduced (75% and 50%) rates of the recommended dose of potassium (RDK) administered through muriate of potash (MOP). Treatments included variations in the incorporation/exclusion of plant growth-promoting rhizobacteria (PGPR), farmyard manure (FYM) at 25% of potassium recommendation, and foliar application of nano potash. The use of 100% RDK +25% K augmentation through FYM + PGPR and nano K fertilizer spray at 25 and 40 DAS (T8) exhibited significant enhancements in green fodder yield (64.0 ± 2.2 t ha-1) over control with no potassium application (47.3 ± 3.7 t ha-1) and found at par with and 75% RDK + 25% K augmentation through FYM + PGPR and nano K fertilizer spray at 25 and 40 DAS (T7). These treatments yielded maximum percent increase for plant height (34.9%), leaf count (38.5%), leaf dimensions (28.8-31.5%), stem girth (25.84%), root volume (27.0%), and root length (37.64%), observed at the harvest stage compared to control (T1-no potassium application). The treatment T8 was on par with T7 and recorded highest uptake of macro (N, P, and K) and micro (Zn, Fe, Cu, and Mn) nutrients. While soil parameters such as available nitrogen and potassium levels were notably increased through the application of treatment T7 across various treatment combinations and found significantly superiority over treatment T8. Multivariate analysis also highlighted treatment T7 is more efficient in maintaining sustainability. Hence, based on the present findings it can be concluded that application of 75% RDK +25% K augmentation through FYM + PGPR and nano K fertilizer spray at 25 and 40 DAS (T7) can be recommended for achieving enhanced productivity and soil fertility improvement within agricultural systems.

Role of calcium nutrition in plant Physiology: Advances in research and insights into acidic soil conditions - A comprehensive review.

Plant mineral nutrition has immense significance for crop productivity and human well-being. Soil acidity plays a major role in determining the nutrient availability that influences plant growth. The importance of calcium (Ca) in biological processes, such as signaling, metabolism, and cell growth, underlines its critical role in plant growth and development. This review focuses on soil acidification, a gradual process resulting from cation leaching, fertilizer utilization, and drainage issues. Soil acidification significantly hampers global crop production by modifying nutrient accessibility. In acidic soils, essential nutrients, such as nitrogen (N), phosphorus (P), potassium (K), magnesium (Mg), and Ca become less accessible, establishing a correlation between soil pH and plant nutrition. Cutting-edge Ca nutrition technologies, including nanotechnology, genetic engineering, and genome sequencing, offer the potential to deliver Ca and reduce the reliance on conventional soluble fertilizers. These fertilizers not only contribute to environmental contamination but also impose economic burdens on farmers. Nanotechnology can enhance nutrient uptake, and Ca nanoparticles improve nutrient absorption and release. Genetic engineering enables the cultivation of acid-tolerant crop varieties by manipulating Ca-related genes. High-throughput technologies such as next-generation sequencing and microarrays aid in identifying the microbial structures, functions, and biosynthetic pathways involved in managing plant nutritional stress. The ultimate goal is to shed light on the importance of Ca, problems associated with soil acidity, and potential of emerging technologies to enhance crop production while minimizing the environmental impact and economic burden on farmers.

Allophane improves anaerobic digestion of chicken manure by alleviating ammonia inhibition and intensifying direct interspecies electron transfer.

Synthesized allophane was employed in anaerobic digestion of chicken manure to improve the stability and methane production under ammonia inhibition. Adding 0.5 %, 1.0 % and 1.5 % (w/w) allophane increased the methane production by 261 âˆ¼ 350 % compared with the group without allophane addition. Further investigation indicated that the maximum adsorption capacity of allophane for NH4+-N achieved at 261.9 mg/g; it suggested that allophane adsorption potentially alleviated the ammonia inhibition, which also was reflected by the increase in the activity of the related enzyme, such as coenzyme F420. Moreover, allophane addition also intensified the direct interspecies electron transfer (DIET) in anaerobic digestion; it can be well supported by the increased relative abundance of Methanosaeta and Methanosarcina involved in the DIET. Overall, the improved anaerobic digestion via alleviating ammonia inhibition and intensifying DIET by allophane was elucidated comprehensively, which can contribute to the development of a functional additive for efficient anaerobic digestion in practical application.

The way forward to produce nutraceuticals from agri-food processing residues: obstacle, solution, and possibility.

Food matrices contain bioactive compounds that have health benefits beyond nutritional value. The bulk of bioactive chemicals are still present in agro-industrial by-products as food matrices. Throughout the food production chain, there is a lot of agro-industrial waste that, if not managed effectively, could harm the environment, company, and how nutritiously and adequately people eat. It's important to establish processes that maximise the use of agro-industrial by-products, such as biological technologies that improve the extraction and acquisition of bioactive compounds for the food and pharmaceutical industries. As opposed to nonbiological processes, biological procedures provide high-quality, bioactive extracts with minimum toxicity and environmental impact. Fermentation and enzymatic treatment are biological processes for obtaining bioactive compounds from agro-industrial waste. In this context, this article summarises the principal bioactive components in agro-industrial byproducts and the biological methods employed to extract them. In this review efficient utilization of bioactive compounds from agro-industrial waste more effectively in food and pharmaceutical industries has been described.

Bioactives from citrus food waste: types, extraction technologies and application.

The Citrus fruits belong to the category where the groups of fruits are recognized to be an admirable repository of bioactive elements and phytochemical constituents, with strong biological potentials. The prominent use of Citrus fruits for nutrition as well as food processing has led to the release of a large amount of waste into the environment and surrounding, and it simultaneously burdens the nature and existence of many organisms including the human population. In order to rectify such consequences, the reuse of food waste from citrus for various advantageous effects. In this regard, the first part of the article primarily focussed on the various strategies available for the extraction of chemical elements from citrus waste and the remaining strand of the article focussed on the various bioactive compounds with special reference to their pharmacological as well as the medicinal benefits and future prospects.

Utilising standard samples instead of randomly collected food waste in composting: Implementation strategy and feasibility evaluation.

Randomly collected food waste results in inaccurate experimental data with poor reproducibility for composting. This study investigated standard food waste samples as replacements for randomly collected food waste. A response surface methodology was utilised to analyse data from a 28-day compost process optimisation experiment using collected food waste, and the optimal combination of composting parameters was derived. Experiments using different standard food waste samples (high oil and salt, high oil and sugar, balanced diet, and vegetarian) were conducted for 28 days under optimal conditions. The ranking of differences between the standard samples and collected food waste was vegetarian > balanced diet > high oil and sugar > high oil and salt. Statistical analysis indicated t-tests for increased oil and salt samples and collected food waste were not significant, and Cohen's d effect values were minimal. High oil and salt samples can be used as replacements for collected food waste in composting experiments.

Biochemical engineering for elemental sulfur from flue gases through multi-enzymatic based approaches - A review.

Flue gases are the gases which are produced from industries related to chemical manufacturing, petrol refineries, power plants and ore processing plants. Along with other pollutants, sulfur present in the flue gas is detrimental to the environment. Therefore, environmentalists are concerned about its removal and recovery of resources from flue gases due to its activation ability in the atmosphere to transform into toxic substances. This review is aimed at a critical assessment of the techniques developed for resource recovery from flue gases. The manuscript discusses various bioreactors used in resource recovery such as hollow fibre membrane reactor, rotating biological contractor, sequential batch reactor, fluidized bed reactor, entrapped cell bioreactor and hybrid reactors. In conclusion, this manuscript provides a comprehensive analysis of the potential of thermotolerant and thermophilic microbes in sulfur removal. Additionally, it evaluates the efficacy of a multi-enzyme engineered bioreactor in this process. Furthermore, the study introduces a groundbreaking sustainable model for elemental sulfur recovery, offering promising prospects for environmentally-friendly and economically viable sulfur removal techniques in various industrial applications.

Investigation on the physico-chemical properties of soil and mineralization of three selected tropical tree leaf litter.

Plant leaf litter has a major role in the structure and function of soil ecosystems as it is associated with nutrient release and cycling. The present study is aimed to understand how well the decomposing leaf litter kept soil organic carbon and nitrogen levels stable during an incubation experiment that was carried out in a lab setting under controlled conditions and the results were compared to those from a natural plantation. In natural site soil samples, Anacardium. occidentale showed a higher value of organic carbon at surface (1.14%) and subsurface (0.93%) and Azadirachta. indica exhibited a higher value of total nitrogen at surface (0.28%) and subsurface sample (0.14%). In the incubation experiment, Acacia auriculiformis had the highest organic carbon content initially (5.26%), whereas A. occidentale had the highest nitrogen level on 30th day (0.67%). The overall carbon-nitrogen ratio showed a varied tendency, which may be due to dynamic changes in the complex decomposition cycle. The higher rate of mass loss and decay was observed in A. indica leaf litter, the range of the decay constant is 1.26-2.22. The morphological and chemical changes of soil sample and the vermicast were substantained using scanning electron microscopy (SEM) and Fourier transmission infrared spectroscopy (FT-IR).

Performance of aerobic denitrifying fungal community for promoting nitrogen reduction and its application in drinking water reservoirs.

The effect of mix-cultured aerobic denitrifying microorganisms on the water remediation has been extensively explored, but little is known about the performance of mix-cultured low efficiency fungi on denitrification. In this study, two kinds of aerobic denitrifying fungi (Trichoderma afroharzianum H1 and Aspergillus niger C1) were isolated from reservoirs, improved the capacity by mix-cultured. The results showed a difference between northern and southern reservoirs, the dominants of genera were Cystobasidium and Acremonium. The removals of total nitrogen (TN) was 12.00%, 7.53% and 69.33% in Trichoderma afroharzianum H1, Aspergillus niger C1 and mix-cultured (C1 and H1) under the denitrification medium. The contents of ATP and electron transport system activity in mix-cultured amendment were increased by 42.54% and 67.52%, 1.72 and 2.91 times, respectively. Besides, the raw water experiment indicated that TN removals were 24.05%, 12.66% and 73.42% in Trichoderma afroharzianum H1, Aspergillus niger C1 and mix-cultured. The removals of dissolved organic carbon in mix-cultured were increased 35.02% and 50.46% compared to Trichoderma afroharzianum H1 and Aspergillus niger C1. Therefore, mix-cultured of two low efficiency aerobic denitrifying fungi has been considered as a novelty perspective for mitigation of drinking water source.

Unraveling impacts of inoculating novel microbial agents on nitrogen conversion during cattle manure composting: Core microorganisms and functional genes.

The impacts of microbial agents on nitrogen conversion during composting is still not entirely clear. In this research, a novel microbial agent containing two thermotolerant nitrifying bacteria was identified and its impacts on nitrogen conversion, bacterial structure and functional genes during cattle manure composting were investigated. The results revealed that the inoculation enhancing the maturation of compost, increased the total nitrogen by 13.6-26.8%, reduced NH3 emission and the N2O emission by 24.8-36.1% and 22.7-32.1%, respectively. Particularly, the microbial agents mixed Acinetobacter radioresistens and Bacillus nitratireducens (1:1, treatment group 1) had the best nitrogen preservation effect. Furthermore, the inoculation not only produced diverse diazotroph community but could strength the co-occurrence between core microorganisms to promote nitrogen metabolism. The metagenomic analysis demonstrated that the inoculation decreased the abundance of nitrate reduction gene (nirS, norC, nap and nif), and increased the abundance of hao, thus facilitating nitrification and suppressing NH3 and N2O emission.

Sludge-derived iron-carbon material enhancing the removal of refractory organics in landfill leachate: Characteristics optimization, removal mechanism, and molecular-level investigation.

Mature landfill leachate is a refractory organic wastewater, and needs physical and chemical pretreatments contemporaneously, e.g. iron-carbon micro-electrolysis (IC-ME). In this study, a novel iron-carbon (Fe-C) material was synthesized from waste activated sludge to be utilized in IC-ME for landfill leachate treatment. The pyrolysis temperature, mass ratio of iron to carbon, and solid-liquid ratio in leachate treatment were optimized as 900 °C with 1.59 and 34.7 g/L. Under these optimal conditions, the chemical oxygen demand (COD) removal efficiency reached 79.44 %, which was 2.6 times higher than that of commercial Fe-C material (30.1%). This excellent COD removal performance was indicated to a better mesoporous structure, and uniform distribution of zero-valent iron in novel Fe-C material derived from sludge. The contribution order of COD removal in IC-ME treatment for landfill leachate was proven as coagulation, adsorption, and redox effects by a contrast experiment. The removal of COD includes synthetic organic compounds, e.g. carcinogens, pharmaceuticals and personal care products. The contents of CHO, CHON, and CHOS compounds of dissolved organic matter (DOM) in the leachate were decreased, and both the molecular weight and unsaturation of lipids, lignin, and tannic acids concentration were also reduced. Some newly generated small molecular DOM in the treated leachate further confirmed the existence of the redox effect to degrade DOM in leachate. The total cost of sludge-derived Fe-C material was only USD$ 152.8/t, which could save 76% of total compared with that of commercial Fe-C materials. This study expands the prominent source of Fe-C materials with excellent performance, and deepens the understanding of its application for leachate treatment.

Biochar production and its environmental applications: Recent developments and machine learning insights.

Biochar production through thermochemical processing is a sustainable biomass conversion and waste management approach. However, commercializing biochar faces challenges requiring further research and development to maximize its potential for addressing environmental concerns and promoting sustainable resource management. This comprehensive review presents the state-of-the-art in biochar production, emphasizing quantitative yield and qualitative properties with varying feedstocks. It discusses the technology readiness level and commercialization status of different production strategies, highlighting their environmental and economic impacts. The review focuses on integrating machine learning algorithms for process control and optimization in biochar production, improving efficiency. Additionally, it explores biochar's environmental applications, including soil amendment, carbon sequestration, and wastewater treatment, showcasing recent advancements and case studies. Advances in biochar technologies and their environmental benefits in various sectors are discussed herein.

Influence of biochar on succession of fungal communities during food waste composting.

This study aims to examine the effects of biochar on fungal dynamics during food waste composting. The different dosage of wheat straw biochar from 0 to 15% (0%, 2.5%, 5%, 7.5%, 10%, and 15%) were used as an additive to composting and examined for 42 days. The results showed that Ascomycota (94.64%) and Basidiomycota (5.36%) were the most dominant phyla. The most common fungal genera were Kluyveromyces (3.76%), Candida (5.34%), Trichoderma (2.30%), Fusarium (0.46%), Mycothermus-thermophilus (5.67%), Trametes (0.46%), and Trichosporon (3.38%). The average number of operational taxonomic units were 469, with the greatest abundance seen in the 7.5% and 10% treatments. Redundancy analysis revealed that different concentrations of biochar applied treatments have significantly distinct fungal communities. Additionally, correlation analyses of fungal interactions with environmental elements, performed through a heatmap, also indicate a distinct difference among the treatments. The study clearly demonstrates that 15% of biochar has a positive impact on fungal diversity and improves the food waste composting.

Thermo-hydraulic performance of solar air heater having discrete D-shaped ribs as artificial roughness.

In this paper, the thermo-hydraulic performance of a solar air heater (SAH) duct roughened with discrete D-shaped ribs is numerically investigated using ANSYS Fluent 2020 R2. The numerical investigation is carried out at rib radius to transverse pitch ratio (r/Pt) from 0.1 to 0.35 and longitudinal pitch to rib radius ratio (Pl /r) from 4 to 10 under various operating conditions with Reynolds number (Re) varied from 10,200 to 20,200. The numerical results are validated with previous experimental results for the Nusselt number (Nu) values, and good agreement is found with mean absolute percentage error (MAPE) of 3.6%. Based on the results of the numerical investigation, it was found that the value of Nu and the friction factor (f) decreases with the increase of the value of Pl/r, while the ratio r/Pt is kept constant. From the overall analysis, it is concluded that the optimum results are obtained for r/Pt of 0.25 and Pl/r = 4, and the maximum thermo-hydraulic performance parameter is 1.12. Further correlations are developed for the value of Nu and f for the whole range of r/Pt as 0.10-0.35 and Pl/r as 4-10. According to the developed correlations, the values of Nu are within ± 2% of the results of CFD, while the values of f are within ± 2.7% of the results of CFD.

Evaluation of fungal dynamics during sheep manure composting employing peach shell biochar.

In this study, explored the influence of different proportion (0%, 2.5%, 5%, 7.5%, and 10%) peach shell biochar (PSB) with microbial agents (EM) on the carbon transformation, humification process and fungal community dynamics during sheep manure (SM) composting. And no additives were used as control. The results manifested that the CO2 and CH4 emissions were effectively reduced 8.23%∼13.10% and 17.92%∼33.71%. The degradation rate of fulvic acid increased by 17.12%∼23.08% and the humic acid contents were enhanced by 27.27%∼33.97% so that accelerated the composting. Besides, the dominant fungal phylum was Ascomycota (31.43%∼52.54%), Basidiomycota (3.12%∼13.85%), Mucoromycota (0.40%∼7.61%) and Mortierellomycota (0.97%∼2.39%). Pearson correlation analysis and network indicated that there were different correlations between physicochemical indexes and fungal community under different additive concentrations. In brief, the two modifiers application promoted the SM degradation and affected the fungal community structure.

Microbial dynamics and nitrogen retention during sheep manure composting employing peach shell biochar.

In this study, the effects of peach shell biochar (PSB) and microbial agent (EM) amendment on nitrogen conservation and bacterial dynamics during sheep manure (SM) composting were examined. Six treatments were performed including T1 (control with no addition), T2 (EM), T3 (EM + 2.5 %PSB), T4 (EM + 5 %PSB), T5 (EM + 7.5 %PSB), and T6 (EM + 10 %PSB). The results showed that the additives amendment reduced NH3 emissions by 6.12%∼32.88% and N2O emissions by 10.96%∼19.76%, while increased total Kjeldahl nitrogen (TKN) content by 8.15-9.13 g/kg. Meanwhile, Firmicutes were the dominant flora in the thermophilic stages, while Proteobacteria, Actinobacteriota, and Bacteroidota were the dominant flora in the maturation stages. The abundance of Bacteroidota and Actinobacteriota were increased by 17.49%∼32.51% and 2.31%∼12.60%, respectively, which can accelerate the degradable organic materials decomposition. Additionally, redundancy analysis showed that Proteobacteria, Actinobacteriota, and Bacteroidota were positively correlated with NO3--N, TKN, and N2O, but a negative correlation with NH3 and NH4+-N. Finally, results confirmed that (EM + 10 %PSB) additives were more effective to reduce nitrogen loss and improve bacterial dynamics.

Advanced approaches for resource recovery from wastewater and activated sludge: A review.

Due to resource scarcity, current industrial systems are switching from waste treatment, such as wastewater treatment and biomass, to resource recovery (RR). Biofuels, manure, pesticides, organic acids, and other bioproducts with a great market value can be produced from wastewater and activated sludge (AS). This will not only help in the transition from a linear economy to a circular economy, but also contribute to sustainable development. However, the cost of recovering resources from wastewater and AS to produce value-added products is quite high as compared to conventional treatment methods. In addition, most antioxidant technologies remain at the laboratory scale that have not yet reached the level at industrial scale. In order to promote the innovation of resource recovery technology, the various methods of treating wastewater and AS to produce biofuels, nutrients and energy are reviewed, including biochemistry, thermochemistry and chemical stabilization. The limitations of wastewater and AS treatment methods are prospected from biochemical characteristics, economic and environmental factors. The biofuels derived from third generation feedstocks, such as wastewater are more sustainable. Microalgal biomass are being used to produce biodiesel, bioethanol, biohydrogen, biogas, biooils, bioplastics, biofertilizers, biochar and biopesticides. New technologies and policies can promote a circular economy based on biological materials.

Microbial production of nutraceuticals: Metabolic engineering interventions in phenolic compounds, poly unsaturated fatty acids and carotenoids synthesis.

Nutraceuticals have attained substantial attention due to their health-boosting or disease-prevention characteristics. Growing awareness about the potential of nutraceuticals for the prevention and management of diseases affecting human has led to an increase in the market value of nutraceuticals in several billion dollars. Nevertheless, limitations in supply and isolation complications from plants, animals or fungi, limit the large-scale production of nutraceuticals. Microbial engineering at metabolic level has been proved as an environment friendly substitute for the chemical synthesis of nutraceuticals. Extensively used microbial systems such as E. coli and S. cerevisiae have been modified as versatile cell factories for the synthesis of diverse nutraceuticals. This review describes current interventions in metabolic engineering for synthesising some of the therapeutically important nutraceuticals (phenolic compounds, polyunsaturated fatty acids and carotenoids). We focus on the interventions in enhancing product yield through engineering at gene level or pathway level.

A critical review on valorization of food processing wastes and by-products for pullulan production.

Pullulan is a commercially available exopolymer biosynthesized by Aureobasidium pullulans supplemented with nitrogen, carbon and other vital components through submerged and solid-state fermentation. These nutrients are very expensive and it raises the cost for the production of pullulan. Hence, the need of alternative cost-effective raw materials for its production is a prerequisite. Owing to its unique physicochemical features, pullulan has various applications in the food, pharmacological, and biomedical domains. Food industrial wastes generate a considerable number of by-products which accumulates and has a negative influence on the environment. These by-products are made up of proteins, carbohydrates, and other components, can be employed as substrates for the production of pullulan. The present review briefs on the pullulan production using food processing waste and by-products and the elements that impact it. It provides an insight into versatile applications of pullulan in food industries. Various challenges and future prospects in the field of research on pullulan production have been uncovered.