Assessment of Immune Response and Efficacy of Essential Oils Application on Controlling Necrotic Enteritis Induced by Clostridium perfringens in Broiler Chickens.

Necrotic enteritis (NE) caused by Clostridium perfringens is one of the most important enteric diseases in poultry. The antibacterial activity of two different essential oil (EO) blends against C. perfringens was investigated both in vitro and in vivo. Additionally, the immunological response to EO treatment was assessed. In the in vitro study, the antibacterial activity of EO formulas and commonly used antibiotics was evaluated against C. perfringens using disk diffusion assay, minimum inhibitory concentration (MIC) assay, and minimum bactericidal concentration (MBC) assay. In the in vivo study, NE experimental infection was performed on 440 Ross broiler chicks at 19 days of age for 4 continuous days. The chicks were treated with either EOs or amoxicillin at 22 days of age for 5 continuous days. One day after the end of treatment, the birds' performance was evaluated by calculating the feed conversion ratio. Serum samples from 120 birds were collected to measure the levels of IL-1ß, IFN-γ, IL-8, IL-10, and IL-17. After that, all birds were slaughtered, and their small intestines were subjected to gross and histopathological evaluation. In addition, bacterial counts in the small intestines were evaluated. In the in vitro study, EOs showed higher antimicrobial activities in comparison with antibiotics against C. perfringens. In the in vivo study, birds treated with EOs showed a significant decrease in bacterial counts, a significant decrease in intestinal lesions, and a significant improvement in performance compared with untreated birds (p < 0.05). Moreover, treating birds with EOs directed the immune system toward an anti-inflammatory pathway. None of the treated birds died due to NE compared with the 10% mortality rate in untreated birds. In conclusion, EOs might be an effective and safe alternative to antibiotics in the treatment of chicken NE.

Effect of waterpipe tobacco smoke exposure on the development of metabolic syndrome in adult male rats.

The prevalence of metabolic syndrome is increased worldwide. Tobacco smoking increases the risk of developing metabolic syndrome. Waterpipe tobacco smoking has become a global trend of tobacco consumption and is as common as cigarette smoking. In this study, the effect of waterpipe tobacco smoke (WTS) on the development of metabolic syndrome in rats was evaluated. Adult Wistar rats were exposed for 19 weeks to either fresh air (control) or WTS for 1 hour daily/ 5 days per week (WTS). Central obesity, systolic blood pressure, lipid profile, glucose hemostasis and levels of leptin and adiponectin were evaluated. The WTS exposure increased body weight, abdominal circumference, systolic blood pressure and fasting glucose compared to control animals (P<0.05), consistent with inducing metabolic syndrome. The retroperitoneal fat, lipid profile and levels of insulin, leptin and adiponectin were not affected by WTS exposure (P>0.05). In conclusion, exposure to WTS has detrimental health effects leading to the development of metabolic syndrome in experimental animals.

Dietary inclusion of alternative feedstuffs had no negative effects on hematological and biochemical parameters of growing Awassi lambs.

Dietary inclusion of alternative feedstuffs (AF) could impact animal performance, ruminal ecosystem, and/or blood parameters. The objective of the current trial was to evaluate the effects of dietary inclusion of mixed AF on hematological and biochemical parameters of growing lambs. Male Awassi lambs (n = 27, average BW = 20.0 ± 0.5 kg and average age of 90 days) were randomly assigned to one of three dietary groups (9 lambs/group). Diets were formulated to contain (on DM basis) 0 (CTL; conventional diet without AF), 25% (25AF), or 50% (50AF) of mixed AF (dry bread, carob pods, olive cake, and sesame meal). Blood samples were collected from each lamb before morning feeding at the beginning (7 days after the start), middle (day 35), and end (68 day) of the study. Hematological parameters were not affected (P ≥ 0.11) by treatment diets. Except for urea N and cortisol, dietary treatments had no significant (P ≥ 0.07) effects on serum levels of all of the measured metabolites and enzymes. Lambs fed the 50AF diet had the lowest blood level of urea N and cortisol. The current study demonstrates that dietary inclusion of AF up to 50% did not negatively impact lambs' health assessed with changes in hematological and biochemical parameters.

The Potential Use of Layer Litter in Awassi Lamb Diet: Its Effects on Carcass Characteristics and Meat Quality.

Carcass parameters and meat quality in lambs that consumed diets having layer hen litter (LL) were evaluated in a complete randomized study. Forty-two lambs were allocated equally (14 lambs/treatment diet) into one of three iso-nitrogenous diets for 75 days. To partially replace soybean meal and barley, LL was given at 0 (LL0), 150 (LL150), or 300 g/kg (LL300) of dietary dry matter (DM). At the termination of the trial, the characteristics of carcasses (hot and cold carcass weight, dressing percentage, and carcass cuts) and meat quality (Musculus longissimus linear dimensions, ultimate pH, cooking loss, water holding capacity (WHC), shear force (SF), color coordinates) were measured after slaughtering all lambs. Longissimus muscle weight was greatest (p < 0.05) for the LL150. For the dissected loin, intermuscular fat content was lowest for the LL0 diet. However, subcutaneous fat content was lower (p < 0.05) in the LL300 diet than LL0 and LL150 diets. Rib fat depth and Musculus longissimus area were greater (p < 0.05) for LL150 than L0. No differences were found in meat pH or color parameters among treatments but WHC and SF were lower in L0 lambs than in lambs fed LL containing diets. Cooking loss was greater for the LL300 diet than the LL0 diet. In summary, quality of meat and carcasses data indicate the possibility of inclusion of LL up to 300 g/kg DM to growing Awassi lambs.

The effects of l-Carnosine on development of metabolic syndrome in rats.

AIMS: The prevalence of metabolic syndrome (MetS) is increasing in several countries. The MetS is characterized by the occurrence of at least three of the following risk factors: decreased high-density lipoprotein cholesterol, increased blood pressure, raised fasting blood glucose, elevated triglycerides, and abdominal obesity. There is a growing evidence of the role of l-carnosine in improving lipid profile and enhancement of the antioxidant activity. However, the effects of l-carnosine on development of MetS are unknown. MAIN METHODS: Male Wistar rats were randomly assigned to receive either; conventional diet (control), high-fat high-carbohydrate diet (HFHCD), l-carnosine and conventional diet (L-Car), or l-carnosine and high-fat high-carbohydrate diet (HFHCD and L-Car) for 16 weeks. Central obesity, systolic blood pressure, lipid profile, glucose hemostasis, levels of leptin and adiponectin were evaluated on week 16. KEY FINDINGS: Rats that received HFHCD for 16 weeks showed MetS phenotype such as central obesity, increased blood pressure and glucose, as well as an altered lipid profile (P < 0.05). l-Carnosine supplementation to MetS rats significantly reduced abdominal obesity, blood pressure and glucose, and normalized total cholesterol and low density lipoprotein cholesterol levels (P < 0.05). Insulin, leptin and adiponectin concentrations were not affected by l-Carnosine (P > 0.05). SIGNIFICANCE: l-carnosine has beneficial effects on ameliorating the manifestations of MetS in rats.

Effects of Partial Replacement of Conventional with Alternative Feeds on Nutrient Intake, Digestibility, Milk Yield and Composition of Awassi Ewes and Lambs.

Two experiments were done to assess the effects of alternative feeds (AF; dried distillers grains with solubles, carob pods, olive cake, and bread by-product) on lactating performance of ewes and digestibility and nitrogen (N) retention of lambs. Diets were: no AF (CON); 200 g/kg AF (AF200); and 400 g/kg AF (AF400). In Experiment 1, 27 Awassi ewes were randomly distributed into three groups, and each was fed one of the diets described before (9 ewes/diet). Evaluation of milk composition and yield was performed at the beginning of the experiment and on days 18, 36, and 54. In Experiment 2, 18 Awassi lambs were allocated to the same diets (6 ewe lambs/diet) during a 21-day trial (14 days housed individually in shaded pens and 7 days in metabolic cages). In Experiment 1, no differences in body weight (BW) of the ewes and their lambs were detected. With the exception of neutral detergent fiber intake, which was lower in the AF-containing diets compared with CON, dry matter, crude protein, and acid detergent fiber intake were not affected by dietary treatment. Milk yield and composition was comparable among diets, and the cost of milk production was lower in AF compared to CON diet. In Experiment 2, nutrient digestibility and N retention were not affected by the diet. Results showed the possibility of including different AF in ewe and lamb diets to mitigate production cost without negatively affecting intake, milk yield, and composition, digestibility, and animal welfare and health.

Effects of intramammary infusion of sage (Salvia officinalis) essential oil on milk somatic cell count, milk composition parameters and selected hematology and serum biochemical parameters in Awassi sheep with subclinical mastitis.

AIM: The aims of this study were to evaluate the effects of intramammary infusion of sage (Salvia officinalis) essential oil (EO) on milk somatic cell count (SCC), milk composition parameters and selected hematology and serum biochemical parameters in 20 Awassi ewes affected with subclinical mastitis. MATERIALS AND METHODS: The dried leaves of sage were used to extract the EO by hydrodistillation. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of sage EO against Staphylococcus aureus were determined by the broth dilution method. Ewes were divided randomly into three main groups and received one of the following treatments; Group 1 (n=5): Dimethyl sulfoxide (DMSO) alone (5 ml; 0.2 ml of DMSO in 4.8 ml of saline), Group 2 (n=5): Amoxicillin alone (3 ml), and Group 3 (n=10): Sage EO (5 ml of sage EO solution [0.2 ml DMSO+1 ml EO+3.8 ml sterile saline]). All treatments were administered by intramammary infusion into each teat twice per day for 3 consecutive days. Milk samples for SCC and milk components determination and whole blood samples for hematology and serum biochemical analyses were collected before treatment (T0) and at 24 (T24) and 48 (T48) h after the last treatment. RESULTS: The MIC and MBC of sage EO against S. aureus were 12.5% and 6.1%, respectively. SCC was decreased significantly (p<0.05) at T24 and T48 h in sage EO and amoxicillin treated groups. Milk fat and lactose were increased significantly (p<0.05) in sage EO and amoxicillin treated ewes while no significant changes were observed in the percentages of solids-not-fat, protein and total solids. No significant effects of sage EO treatment on any of the hematology or serum biochemical parameters were observed. There were no local or systemic side effects observed in any of the treated ewes. However, further clinical trials are warranted to determine safety and possible withdrawal times in milk before its recommendation for use in organic operations. CONCLUSION: In this study, the intramammary infusion of sage EO to ewes affected with subclinical mastitis resulted in a significant decrease in SCC 24 h and 48 h posttreatment. In addition, milk fat and lactose were increased in animals that received the EO as well as in those treated with the antibiotic.

Rumen-protected methionine and lysine: effects on milk production and plasma amino acids of dairy cows with reference to metabolisable protein status.

Two experiments were conducted to study the effects of rumen-protected Met (RPM) alone or with rumen-protected Lys (RPL) on milk yield and plasma amino acids of dairy cows. In experiment 1, 24 multiparous Holstein cows (154 DIM) were assigned to one of 3 groups where each cow received 0 g/d of RPM and RPL (C), 30 g/d of RPM (M), or 30 g/d of RPM plus 25 g of RPL (ML). The study lasted for 8 weeks where milk yield and composition were determined weekly. Daily milk yield averaged 28·0, 27·8, and 29·7 kg/cow for the C, M, and ML groups, respectively. Dietary treatments had no effects (P ≥ 0·54) on milk contents of fat, lactose, solid non-fat or total solids. Milk protein content in the ML group was greater (P < 0·05) than the C and M groups. Plasma levels of all AA were not significantly (P ≥ 0·09) affected by supplemental RPL and/or RPM. In experiment 2, 30 multiparous Holstein cows (100 DIM) were assigned to one of 3 groups where each cow received 0 g/d of RPM and RPL (C), 50 g/d of RPM (M), or 50 g/d of RPM plus 25 g/d of RPL (ML). The study lasted for 5 weeks. Cows in the M (30·5 kg) and ML (31·4 kg) groups produced (P < 0·05) more milk than those of the C group (29·1 kg). Under conditions of this study, RPM plus RPL improved milk yield and protein contents of dairy cows and was better than supplying RPM alone. Response in milk yield to RPM and RPL was affected by the MP status of cows which deserves further investigation.

Alternative feedstuffs and their effects on performance of Awassi sheep: a review.

Fat-tailed sheep (FTS) is a group of breeds characterized by large (fatty) tails that it is commonly distributed in Africa, Middle East, Pakistan, and to a lesser extent in other countries. Awassi, a common FTS breed in many Mediterranean countries, is adaptive and suitable to live in harsh conditions of the arid and semiarid areas. One of the main constraints for sheep industry in those areas is the limited supply and variable quality and quantity of feedstuffs. Using several alternative feedstuffs (AF) has been a common practice to decrease production cost of Awassi sheep industry in those areas. The appropriate AF to be used is determined by several animal and feed factors. These AF includes, but not limited to, unconventional feedstuffs, agricultural byproducts, and agro-industrial byproducts. A good body of literature about the use of AF and the effects of such use on performance of Awassi sheep is available. Some of these AF have been shown to be safely used in Awassi diets with no detrimental effects on sheep performance or health and, thus, recommended to lower the production cost. Other AF has controversial effects and recommendations. This paper reviews the effects of using AF on performance of Awassi ewes and lambs. Effects on intake, nutrient digestibilities, growth rate, and carcass characteristics of lambs and on intake, nutrient digestibilities, body weight change, milk yield, and milk composition of ewes will be emphasized. Recommendations and limitations for using AF will also be briefly discussed.

Effect of feeding calcium salts on performance of nursing Awassi ewes.

Twenty nursing Awassi ewes (BW = 50 ± 2.35 kg, age = 4.5 ± 1.2 years) with their lambs were used to evaluate the effects of feeding calcium salts in lactation diets on performance and pre-weaning growth of their lambs. Treatments were 0% calcium salts (CON) or 5% calcium salts (FAT). At the end of the study, a digestibility experiment was performed. Milk yield was greater (P < 0.05) for ewes fed the FAT diet than the CON diet. Milk composition was similar (P > 0.05) between diets. However, milk energy value (kcal/day) tended to be greater (P = 0.07) for the FAT diet than the CON diet. Concentrations of milk C18:1c9 and C20:0 were greater (P < 0.05) in ewes fed the FAT diet than the CON diet. However, concentration of trans-10, cis-12 CLA was lower (P = 0.05) in the FAT diet than in the CON diet. No differences in feed intake and body weight change were detected between diets. Digestibility of dry matter, organic matter, crude protein, ether extract, neutral detergent fiber, and acid detergent fiber were similar (P > 0.05) for diets. For lambs, weaning weight was not affected by treatments. However, average daily gain and total gain were greater (P = 0.053) for the FAT diet than the CON diet. Results suggest that supplementing lactating ewes with calcium salts at the beginning of lactation phase improves daily milk yield of ewes and pre-weaning growth of their lambs with no major negative impact on feed intake and digestibility.