RESUMEN
In spite of the widespread use of proteins (casein, peptone, etc.) and protein fragments as a substrate for the proteolytic enzymes, a substrate prepared from dyes that adsorb onto appropriate materials, such as wool and cotton, are also used for enzyme activity determination. In the point of view of this thought, it was our aim to develop the substrates which are easily and economically obtainable and also environmentally safer for the frequently used proteolytic enzymes, such as subtilisin carlsberg, trypsin, chymotrypsin, and protease type XVI and, if possible, to prepare the specific substrate at least for one of these enzymes. For this aim, wool was dyed with natural dyes such as juglone, lawsone, berberine, and quercetin. The optimum pH, incubation time, and agitation rate were determinated. The results indicate that, of all the tested enzymes on wool-dye complex as an insoluble substrate, the most appropriate complex was found to be wool-lawsone complex.
Asunto(s)
Colorantes/química , Péptido Hidrolasas/metabolismo , Lana/química , Lana/metabolismo , Animales , Quimotripsina/metabolismo , Colorantes/análisis , Concentración de Iones de Hidrógeno , Pronasa/metabolismo , Ovinos , Especificidad por Sustrato , Subtilisina/metabolismo , Tripsina/metabolismoRESUMEN
The activity of proteolytic enzymes is commonly measured using casein as a substrate. A modified caseinolysis assay was developed with natural dyes such as juglone, lawsone, berberine, and quercetin for Subtilisin carlsberg, protease type XVI, and trypsin, respectively. The pH dependence and incubation time were determined. K(m), V(max), and k(cat)/K(m) values were also determined for these enzymes. Lawsone was found to be a better substrate than the others.
Asunto(s)
Caseínas/química , Colorantes/química , Péptido Hidrolasas/química , Concentración de Iones de Hidrógeno , Hidrólisis , Especificidad por SustratoRESUMEN
The essential oils of Hypericum scabrum, Hypericum scabroides and Hypericum triquetrifolium were studied for the first time for their antimicrobial activity against nine organisms. All the essential oils exhibited some broad spectrum antibacterial activity, at a concentration of 80 microg/mL. The essential oils of Hypericum species showed antibacterial activity against the tested organisms and a yeast.
Asunto(s)
Antiinfecciosos/farmacología , Bacterias Gramnegativas/efectos de los fármacos , Bacterias Grampositivas/efectos de los fármacos , Hypericum , Fitoterapia , Aceites de Plantas/farmacología , Antiinfecciosos/administración & dosificación , Antiinfecciosos/uso terapéutico , Antifúngicos/administración & dosificación , Antifúngicos/farmacología , Antifúngicos/uso terapéutico , Candida albicans/efectos de los fármacos , Flores , Humanos , Pruebas de Sensibilidad Microbiana , Hojas de la Planta , Aceites de Plantas/administración & dosificación , Aceites de Plantas/uso terapéutico , Tallos de la PlantaRESUMEN
The antimicrobial activity of the ethanol extract of resins obtained from the roots and stems of Cedrus libani and Abies cilicica was investigated using a disc diffusion method. Results revealed that crude extracts of the resins of both plants are highly effective against tested microorganisms by preventing their growth to a greater extent.
