Clinical Scenario and Imaging with Illustrations of Giant Cell Tumor of Bone: A Retrospective Analysis.

Background: The giant cell tumour of the bone has a spectrum of clinical-radiological presentation. This study aims to describe this varied presentation in our institution. Methods: This retrospective study was conducted on twenty-nine pathologically labelled cases of giant cell tumours of the bone. The medical records for their clinical presentation and diagnostic imaging studies were studied and evaluated. Results: Mean age of the patients at presentation was 35.3±12.9 years. Pain, local swelling and restricted joint function were seen in 93 %, 58.6 % and 52 % patients, respectively. The cortical breach was seen in 15 (51.7 %) and 22 (75.9 %) lesions on plain radiographs and CT images, respectively. 14(48.3 %) cases had soft tissue invasion on MRI at presentation. 26 (89.7 %) lesions were located within 1 cm from the articular cartilage. The solid tumour component was hypo to iso-intense in signal intensity in 27 (93.1 %) lesions in T1 weighted and 21 (72.4 %) in T2 weighted images. 14 (48.3 %) had hyperintense cystic areas, and fluid-fluid levels, suggestive of aneurysmal bone cysts, were seen in 4 (13.8 %) cases on T2 weighted images. Hypo-echoic nodular areas in solid tumour component, suggestive of hemosiderin deposits, were present in 3 (10.3 %) lesions on T1 and T2 weighted images. Conclusion: The tumour classically presents as an epiphysial-metaphyseal, eccentric, expansile, lytic lesion in a skeletally mature patient. The MRI picture is variable and the surgeon should have a sound knowledge of these variations to obtain a biopsy sample from a proper site of the lesion and to avoid misdiagnosis especially of a primary ABC.

Idebenone improves quality of ram sperm by mitigating oxidative stress during cryopreservation.

The present study was designed to test the effect of different levels of idebenone, a potent antioxidant on the quality of ram semen at post thaw. Eighteen (18) ejaculates were collected and extended with tris extender supplemented with no antioxidant (CON), with 2 µM idebenone (Id2), 5 µM idebenone (Id5), 7.5 µM idebenone (Id7.5) and 10 µM idebenone (Id10). The sperm quality was determined in terms of percent sperm motility, live sperm percentage, percent hypoosmotic swelling test (HOST) positive spermatozoa and percent intact acrosome (PIA). Moreover, malondialdehyde (MDA) level, an end product of lipid peroxidation (LPO) was also measured at post thaw both in seminal plasma and sperm cell. At post thaw, the percent sperm motility was significantly higher (p < 0.05) for Id10 as compared to Id2, Id5, Id7.5 and control. The live sperm percentage was non-significantly (p > 0.05) higher for Id10 as compared to control, Id5 and Id7.5 but significantly higher than Id2. The percent HOST positive spermatozoa was significantly higher (p < 0.05) for Id10 than control, Id2 and Id5. The MDA level in seminal plasma was significantly lower (p < 0.05) for Id10 than control and Id2. The MDA level in spermatozoa did show similar trend as in seminal plasma. Further, all the sperm parameters at all idebenone levels declined significantly from pre freeze to post thaw. In conclusion, idebenone at 10 µM level improved post thaw sperm quality by mitigating peroxidative stress, hence could be considered as a promising antioxidant additive for cryopreservation of ram semen.

The Effect of Precooling on Exhaustive Performance in the Hot Environment.

BACKGROUND: Pre-cooling is known to enhance exercise performance in soccer players. However, little information currently exists regarding precooling effects in Iranian young soccer players. OBJECTIVES: The aim of this study was to assess the effect of precooling (water immersion) on exhaustive performance in the heat ( temperature = 32 - 34°C, humidity = 50%). PATIENTS AND METHODS: Sixteen young male soccer players from the provincial competitive teams were divided into two equal groups and were randomly assigned to precooling (age = 16.5 ± 1.1 year, height = 171.7 ± 6.4 cm, BMI = 21.5 ± 3.3, VO2max = 50.6 ± 6.9 mL/kg/min) and non-precooling (age = 16.1 ± 1.1 year, height = 170.0 ± 4.7 cm, BMI = 21.3 ± 3.6, VO2max = 50.6 ± 6.8 mL/kg/min) groups. An exhaustive treadmill run test was conducted after warm-up (non-precooling) or warm-up + water immersion (temperature = 22 - 24°C). Oral temperature, plasma lactate and plasma volume were measured at the baseline (fasting state), mid test (immediately after warm up or warm -up + water immersion) and post test (immediately after exhaustive test). Mixed repeated measures analysis of variance and independent t test were used for data analyzing. P < 0.05 was considered significant. RESULTS: There were no significant differences between two groups at baseline, mid test and post test regarding oral temperature and plasma lactate. The time to exhaustion was considerably higher in the precooling group compared with the non-precooling group, but the difference was not statistically significant. No significant differences were found between the two groups on measures of the baseline and mid test plasma volume, but post test plasma volume was significantly higher in the precooling group compared to the non-precooling group (P < 0.05). CONCLUSIONS: These results show that precooling effectively attenuates dehydration, but has no positive effect on exhaustion time in the hot environment.

BAY 41-2272 Treatment Improves Acetylcholine-Induced Aortic Relaxation in L-NAME Hypertensive Rats.

Hypertension, an emerging problem of recent era, and many pathophysiological factors are participating to produce the disease. Nitric oxide (NO) is an important constituent to ameliorate hypertensive condition. Inhibition of endogenous NO synthase by L-NG-Nitroarginine methyl ester (L-NAME) was responsible for generating hypertension in rats. BAY 41-2272 (5-cyclopropyl-2-[1-(2-fluoro-benzyl)-1H-pyrazolo[3,4-b]pyridine-3-yl]-pyrimidin-4-ylamine), a soluble guanylyl cyclase activator, restricts rise of blood pressure and shows cardioprotective activity. The aim of the present study was to analyze effect of short-term BAY 41-2272 treatment on blood pressure and vascular function. Male Wistar rats were randomly divided into three groups such as control (group-A), hypertensive (group-B), and BAY 41-2272-treated hypertensive (group-C) rats. Normal saline was administered intramuscularly to control rats for last 3 days (days 40, 41, and 42) of total 42 days treatment, whereas rats of group-B and group-C were treated with L-NAME hydrochloride in drinking water at 50 mg/kg body weight daily for 42 days. Also, normal saline and BAY 41-2272 were administered for last 3 days at two different dosages at 1 and 3 mg/kg body weight/day intramuscularly to group-B and group-C rats, respectively. Administration of BAY 41-2272 for 3 days was not sufficient enough to decrease mean arterial pressure of hypertensive rats significantly. BAY at both the treatment dosages significantly ameliorate acetylcholine-induced maximal aortic relaxation compared with BAY-untreated hypertensive rats. Findings of the present study indicate that even shorter period of BAY 41-2272 treatment (3 days) improves vascular relaxation.

Influence of Endurance Exercise Overloading Patterns on the Levels of Left Ventricular Catechoamines After a Bout of Lactate Threshold Test in Male Wistar Rat.

BACKGROUND: It is well known that exercise training has positive effect on catecholamine response to a given work load. But in this regard, the effective method of training needs to be studied. OBJECTIVES: The aim of this study was to compare the effects of 8 weeks endurance exercise with two overloading patterns on the left ventricular catecholamine levels. MATERIALS AND METHODS: 29 male Wistar rats were randomly assigned to control (n = 9), daily sinusoidal overloading (n = 10) and weekly sinusoidal overloading (n = 10) groups. After the last exercise session, left ventricular blood samples were obtained immediately after lactate threshold test. Plasma concentrations of adrenaline and noradrenaline were measured by ELISA method. One way analysis of variance was used for analysis of the data. RESULTS: Immediately after lactate threshold test, adrenaline level was significantly (P < 0.05) lower in weekly loading group than in control and daily loading groups. Adrenaline was higher in the daily loading group compared with control group but did not reach the significant level. Noradrenaline levels were not significantly (P > 0.05) different between three study groups. CONCLUSIONS: The results showed 8 weeks of endurance exercise with weekly sinusoidal overloading pattern could induce a lower adrenal medulla activity (reflection of physical and physiological improvement) than daily sinusoidal loading pattern in response to the same absolute work load.

Lean body mass can predict lung function in underweight and normal weight sedentary female young adults.

BACKGROUND: A previous cross-sectional study found reduced lung function among over weight and obese students in Zanjan city. However, there is no reliable evidence about the respiratory function of underweight and normal weight students. The objective of this study was to evaluate lung function and develop prediction equations in underweight and normal weight female young adults residing in Soltanieh city near Zanjan. MATERIALS AND METHODS: A cross sectional study was conducted on underweight (n=29, mean age=16±0.84 years) and normal weight (n=38, mean age=15.9±0.86 years) sedentary female young adults. Fat mass (FM) was measured by Omron Body Fat Monitor FB-3002. Lean body mass (LBM), waist to hip ratio (WHR), body mass index (BMI) and waist circumference (WC) were calculated. Maximum oxygen uptake was calculated using Queen's College step test. Forced vital capacity (FVC) and forced expiratory volume in one second (FEV1) were recorded, using Spirolab III spirometer. Independent t test, Pearson's correlation test and stepwise linear regression analysis were used for data analysis. RESULTS: FVC and FEV1 were significantly lower than the reference values in both groups (P<0.05). LBM was significantly correlated with FVC and FEV1 in underweight and normal weight groups (P<0.05). Regression equations were derived to predict FVC and FEV1 using LBM. CONCLUSION: This study suggests that dynamic lung functions are poor in underweight and normal weight sedentary female young adults residing in Soltanieh city and LBM plays a significant role in their lung function.

Wound healing activity of carbon monoxide liberated from CO-releasing molecule (CO-RM).

Wound microenvironment presents widespread oxidant stress, inflammation, and onslaught of apoptosis. Carbon monoxide (CO) exerts pleiotropic cellular effects by modulating intracellular signaling pathways which translate into cellular protection against oxidative stress, inflammation, and apoptosis. CO-releasing molecules (CO-RMs) deliver CO in a controlled manner without altering carboxyhemoglobin levels. This study observed a potential therapeutic value of CO in the wound healing by using tricarbonyldichlororuthenium (II) dimer (CO-releasing molecule (CO-RM)-2), as one of the novel CO-releasing agent. The effect of CO-RM-2 treatment was studied on wound contraction, glucosamine, hydroxyproline levels, and mRNA of cytokines/adhesion molecule in rats using a full-thickness cutaneous wound model and angiogenesis in chick chorioallantoic membrane (CAM) model. CO-RM-2 treatment increased cellular proliferation and collagen synthesis as evidenced by the increase in wound contraction and hydroxyproline and glucosamine contents. The mRNA expression of cytokines endorsed fast healing, as was indicated by the inhibition of pro-inflammatory adhesion molecules such as ICAM-1 and cytokine TNF-α and upregulation of anti-inflammatory cytokine IL-10. An ELISA assay of IL-10 and TNF-α cytokines revealed pro-healing modulation in excision wound by CO-RM-2 treatment. CO-RM significantly promoted the angiogenesis as compared to the iCO-RM group in vitro in CAM model demonstrating pro-angiogenic effects of CO-RM-2 in wound healing process. These results indicate that CO-RM-2 may have a potential application in the management of recalcitrant/obstinate wounds wherein, active wound healing is desired. This study also opens up a new area of research for the synthesis of novel CO-releasing molecules to be used for such purposes.

Effects of aerobic exercise on lung function in overweight and obese students.

BACKGROUND: In healthy teenagers, obesity and physical inactivity are the two main factors that affect respiratory function. The aim of this study was to evaluate the effect of aerobic exercise training on improving lung function in overweight and obese students. MATERIALS AND METHODS: Thirty overweight or obese subjects with poor endurance performance and mild deterioration of respiratory indices (forced expiratory volume and forced expiratory volume in 1 second < 90% predicted) were randomly assigned into control (age: 16.6±0.83 years, height: 167±5.05 cm, weight: 80.44±7.65 kg) and intervention groups (age: 16.5±0.83 years, height: 166±6.7 cm, weight: 79.62±9.33 kg). The intervention group preformed 24 weeks of continuous treadmill running (3 days a week). Respiratory indices were measured pre, mid and post exercise. Independent t test, paired t test, Pearson's correlation test and repeated measure were used for analyzing the data. RESULTS: In the intervention group, post exercise respiratory indices were significantly higher than the pre exercise values, and did not reach the predicted values. No significant differences were found in pre, mid and post exercise respiratory indices in the control group. In the intervention group, improvements in respiratory indices were positively correlated with maximum voluntary ventilation (MVV) improvement but not with BMI reduction. No significant differences were detected between the 2 groups in terms of pre, mid and post exercise measures of BMI, weight, height, and respiratory indices. CONCLUSION: In overweight and obese teenagers, appropriate aerobic exercise training can partly improve lung function by strengthening the muscles of respiration. However, in order to achieve the predicted values of lung function, a further increase in activity duration and decrease in BMI is necessary.

Pharmacokinetics of amikacin in plasma of healthy goats after intravenous injection once daily for three days / 대한수의학회지

Amikacin is a semisynthetic derivative of kanamycin and primarily active against aerobic Gram-negative-pathogens with limited activity against Gram-positive bacteria. Meager study was reported on pharmacokinetic data on multi-days administration of amikacin. Hence, pharmacokinetics study was done in five clinically healthy goats (n = 5), after intravenous bolus injection of amikacin sulfate at the dose rate of 10 mg/kg body weight daily for three consecutive days. The amikacin concentrations in plasma and pharmacokinetics-parameters were analyzed by using microbiological assay technique and noncompartmental open-model, respectively. The mean peak plasma concentrations (Mean +/- SD) of amikacin at time zero (Cp0) was 114.19 +/- 20.78 and 128.67 +/- 14.37 microg/mL, on day 1st and 3rd, respectively. The mean elimination half-life (t(1/2)ke) was 1.00 +/- 0.28 h on day 1st and 1.22 +/- 0.29 h on day 3rd. Mean of area under concentration-time curve (AUC(0-->infinity)) was 158.26 +/- 60.10 and 159.70 +/- 22.74 microg.h/mL, on day 1st and 3rd respectively. The total body clearance (ClB) and volume of distribution at steady state (Vdss) on day 1st and 3rd were ClB = 0.07 +/- 0.02 and 0.06 +/- 0.01 L/h.kg and Vdss = 0.10 +/- 0.03 and 0.11 +/- 0.05 L/kg, respectively. No-significant difference was noted in both drug-plasma concentration and pharmacokinetics-parameters, respectively. Amikacin concentration in plasma was found higher up-to 4 h and 6 h onward on down-ward trends favour to reduce toxicity. Which also support the pharmacokinetic-pharmacodynamic way of dosing of aminoglycosides and hence, amikacin may be administered 10 mg/kg intravenously daily to treat principally Gram-negative pathogens and limitedly Gram-positive-pathogens.