Clinical trials of new culture media for staphylococcus isolation.

A comparative analysis of the quality of the developed nutrient media, Baird-Parker dry agar base and Vogel-Johnson dry agar Base and foreign analogues, was done based on results of clinical trials. The tested media were qualified by the main biological parameters, such as sensitivity, growth rate, and differentiating and inhibiting properties. The evaluation of statistical reliability of the results of trials of clinical samples was evaluated taking into account the number of parallel studies and the number of matches of the results of studies conducted by different performers. 116 clinical samples of received by a laboratory of the testing laboratory center for research from hospital no.164 over the period of clinical trials were analyzed. 46 cultures of potential pathogens were isolated when culturing on test and control media: S. aureus -35; S. epidermidis-6; S. saprophyticus - 5. Lecithinase activity on the medium "Baird-Parker dry agar Base" and mannitol fermentation on the medium "Vogel-Johnson dry agar Base" in the preliminary phenotypic test allow the isolation and differentiation of clinical isolates of S. aureus from S. epidermidis, and S. saprophyticus.

[Clinical trials of salmonella enrichment medium.]

Salmonella infections continue to be a serious problem in modern medicine. Being intestinal infections-associated pathogens, representatives of the genus Salmonella manifest themselves as pathogenic bacteria, especially in developing nosocomial infections. Given the polymorphism of clinical symptoms of salmonellosis, laboratory studies using bacteriological and serological methods are an important link in the diagnosis. In addition, the general prevention of salmonellosis includes measures to identify bacteria carriers, to ensure control over the incidence in farm animals and birds, food safety, etc. The list of nutrient media to isolate and identify Salmonella is lengthy and steadily extending, and the choice of specific media is largely relies on the nature of the material under study as well as on the idea of the potential availability of Salmonella bacteria in it, with research, diagnosis or epidemic situation being taken into account. The SRCAMB (Rospotrebnadzor) has designed two nutrient media allowing the enrichment and isolation Salmonella from various clinical samples. These are "Nutrient Medium for Enrichment of Salmonella, Dry (Magnesium medium) and "Nutrient Agar with Brilliant Green, Phenolic Red, Lactose and Sucrose, Dry (BPLS-FMH agar)." Growth and inhibitory properties of the new culture media produced by the SRCAMB and commercial domestic and foreign counterparts have been compared by using clinical material. Domestic nutrient media such as Magnesium medium and BPLS-FMH agar were proved to correspond to their commercial analogues when being used for enrichment, isolating and counting Salmonella bacteria in clinical specimens to have bacteriological control real data.

[The clinical trials of agar and Mossel EE broth.]

The cultural diagnostic of acute intestinal infections is based on application of growth medium for selective cumulation of enterobacteria. The development of composition and technology of production of national import-substituting growth medium is an important task for supporting laboratories with high quality medical products. In the state research center of applied microbiology and biotechnology are developed and registered in established procedure a dry growth medium for selective cumulation of enterobacteria (Mossel EE broth) and dry growth medium for selective isolation and counting of enterobacteria (Mossel agar). Both media are intended for selective cumulation, isolation and counting of enterobacteria of family Enterobacteriaceae in clinical material and other objects. The comparative evaluation was applied concerning growth and inhibiting characteristics of new growth media produced by the state research center of applied microbiology and biotechnology against foreign analogues using clinical material. The total correspondence of national Mossel broth and Mossel agar to foreign analogues in case of using these media with the purpose of selective cumulation, isolation and counting of enterobacteria of family Enterobacteriaceae in clinical material and obtaining objective results of bacteriolog.