RESUMEN
Huntington's disease is caused by a CAG trinucleotide expansion mutation in the Huntingtin gene that leads to an artificially long polyglutamine sequence in the Huntingtin protein. A key feature of the disease is the intracellular aggregation of the Huntingtin exon 1 protein (Httex1) into micrometer sized inclusion bodies. The aggregation process of Httex1 has been extensively studied in vitro, however, the crucial early events of nucleation and aggregation in the cell remain elusive. Here, we studied the conformational dynamics and self-association of Httex1 by in-cell experiments using laser-induced temperature jumps and analytical ultracentrifugation. Both short and long polyglutamine variants of Httex1 underwent an apparent temperature-induced conformational collapse. The temperature jumps generated a population of kinetically trapped species selectively for the longer polyglutamine variants of Httex1 proteins. Their occurrence correlated with the formation of inclusion bodies suggesting that such species trigger further self-association.
Asunto(s)
Proteína Huntingtina/química , Proteína Huntingtina/metabolismo , Agregación Patológica de Proteínas/fisiopatología , Proteína Huntingtina/genética , Enfermedad de Huntington/fisiopatología , Técnicas In Vitro , Cuerpos de Inclusión/metabolismo , Rayos Láser , Modelos Moleculares , Mutación , Péptidos/química , Pliegue de Proteína , Estructura Terciaria de Proteína , Temperatura , UltracentrifugaciónRESUMEN
Micro composites are commonly characterized in bulk. Here we study the temperature triggered release of a bioactive compound from single isolated microcapsules. We monitor the release process in real-time using a novel thermal microscopy method combining laser-induced heating and fluorescence imaging.