New insights in food security and environmental sustainability through waste food management.

Food waste has been identified as one of the major factors that constitute numerous anthropogenic activities, especially in developing countries. There is a growing problem with food waste that affects every part of the waste management system, from collection to disposal; finding long-term solutions necessitates involving all participants in the food supply chain, from farmers and manufacturers to distributors and consumers. In addition to food waste management, maintaining food sustainability and security globally is crucial so that every individual, household, and nation can always get food. "End hunger, achieve food security and enhanced nutrition, and promote sustainable agriculture" are among the main challenges of global sustainable development (SDG) goal 2. Therefore, sustainable food waste management technology is needed. Recent attention has been focused on global food loss and waste. One-third of food produced for human use is wasted every year. Source reduction (i.e., limiting food losses and waste) and contemporary treatment technologies appear to be the most promising strategy for converting food waste into safe, nutritious, value-added feed products and achieving sustainability. Food waste is also employed in industrial processes for the production of biofuels or biopolymers. Biofuels mitigate the detrimental effects of fossil fuels. Identifying crop-producing zones, bioenergy cultivars, and management practices will enhance the natural environment and sustainable biochemical process. Traditional food waste reduction strategies are ineffective in lowering GHG emissions and food waste treatment. The main contribution of this study is an inventory of the theoretical and practical methods of prevention and minimization of food waste and losses. It identifies the trade-offs for food safety, sustainability, and security. Moreover, it investigates the impact of COVID-19 on food waste behavior.

Seasonal fluctuation of water quality and ecogenomic phylogeny of novel potential microbial pollution indicators of Veshaw River Kashmir-Western Himalaya.

Nearly a billion people, especially in underdeveloped nations, need safe drinking water. Indian studies suggest that most drinking water sources have high coliform levels, and quality assurance is required. This study was conducted in rural parts of South Kashmir in the Western Himalaya from February 2019 to January 2020. Standard river water sampling was done from upstream to downstream of the river. This study examined the detection, molecular identification, and chemical water quality of coliform-contaminated drinking water, which sums up river water pollution. Water quality varied significantly, indicating downstream contamination. Sangam (downstream) had the highest coliform count, showing 72.2600 cfu per litre in summer, while Kongwaton (upstream), near the Veshaw River, had no coliform count in winter. In summer, Sangam (downstream) had the highest water quality metrics (pH 6.847, Electrical conductivity (EC) 71.620 dS/m, Biological oxygen demand (BOD) 1.120 mg/L, and Chemical oxygen demand (COD) 24.637 mg/L) in all seasons. The lowest winter water quality metrics in Kongwaton were pH 8.947, EC 253.680 dS/m, BOD 4.963 mg/L, and COD 51.440 mg/L. Coliforms in water suggest faecal contamination. This study examines the water quality attributes of drinking water and associated factors to determine river pollution. Total DNA was collected and sequenced for 16 S rDNA and metagenomics. Universal primers were used to amplify the bacterial 16 S rRNA. Using BLAST, the amplified 16 S rRNA gene sequence was matched to the NCBI database. A metagenomic study revealed 27 species with different relative abundance. These species include Escherichia coli, E. fergusonii, E. albertii, Klebsiella grimontii, and Shigella dysenteriae. This study is thought to be the first to discriminate against E. fergusonii, E. albertii, K. grimontii, and S. dysenteriae from E. coli and to report on E. fergusonii and E. albertii, K. grimontii, and S. dysenteriae in the river Veshaw water sources in Kulgam, Western Himalaya.

Dynamics, phylogeny and phyto-stimulating potential of chitinase synthesizing bacterial root endosymbiosiome of North Western Himalayan Brassica rapa L.

The less phytopathogen susceptibility in Himalayan Brassica rapa L. has made it an exceptional crop eluding synthetic pesticide inputs, thereby guarantying economically well-founded and ecologically sustainable agriculture. The relevance of niche microflora of this crop has not been deliberated in this context, as endosymbiosiome is more stable than their rhizosphere counterparts on account of their restricted acquaintance with altering environment; therefore, the present investigation was carried out to study the endophytic microfloral dynamics across the B. rapa germplasm in context to their ability to produce chitinase and to characterize the screened microflora for functional and biochemical comportments in relevance to plant growth stimulation. A total of 200 colonies of bacterial endophytes were isolated from the roots of B. rapa across the J&K UT, comprising 66 locations. After morphological, ARDRA, and sequence analysis, eighty-one isolates were selected for the study, among the isolated microflora Pseudomonas sp. Bacillus sp. dominated. Likewise, class γ-proteobacteria dominated, followed by Firmicutes. The diversity studies have exposed changing fallouts on all the critical diversity indices, and while screening the isolated microflora for chitinase production, twenty-two strains pertaining to different genera produced chitinase. After carbon source supplementation to the chitinase production media, the average chitinase activity was significantly highest in glycerol supplementation. These 22 strains were further studied, and upon screening them for their fungistatic behavior against six fungal species, wide diversity was observed in this context. The antibiotic sensitivity pattern of the isolated strains against chloramphenicol, rifampicin, amikacin, erythromycin, and polymyxin-B showed that the strains were primarily sensitive to chloramphenicol and erythromycin. Among all the strains, only eleven produced indole acetic acid, ten were able to solubilize tricalcium phosphate and eight produced siderophores. The hydrocyanic acid and ammonia production was observed in seven strains each. Thus, the present investigation revealed that these strains could be used as potential plant growth promoters in sustainable agriculture systems besides putative biocontrol agents.

Impact of Anthropogenic Pressure on Physico-chemical Characteristics of Forest Soils of Kashmir Himalaya.

The present study was carried out during the years 2017-2018 and assessed the impact of anthropogenic activities on the physico-chemical characteristics of soil in the Kashmir Himalaya. At disturbed sites anthropogenic activities like deforestation, grazing, tourism, urbanization, traffic etc. are seen prominent and their effect on soil environment resulted in less vegetation cover and exhibited diminution in organic matter. There was a significant increase in moisture content (21.13 ± 1.51), organic carbon (2.65% ± 0.52%), available nitrogen (493.790 ± 2.105 kg/ha), and potassium (432.727 ± 1.738 kg/ha) at undisturbed area Baerabal Harwan. However, there was a reduced pH (5.39 ± 0.230) and available phosphorus (18.993 ± 1.370) at undisturbed area in contrast to disturbed sites. Significantly higher values of Fe, Cu, Zn and Ni were found in disturbed areas (46.33 ± 0.16, 3.972 ± 0.001, 2.224 ± 0.003 and 1.7033 ± 0.002 ppm) respectively. The present findings could be helpful in formulating conservation strategies of soil at disturbed areas that are affected by anthropogenic activities which effects the soil microbial health of the forest soils. The study therefore indicated the need for employing best forest management and effective enforcement with vigorous reforestation programmes and would be a way forward towards mitigating the ongoing deterioration of the plant-soil system, sustaining forest productivity and soil fertility in the long run, and protecting people's livelihoods.

Bacterial Endophyte Community Dynamics in Apple (Malus domestica Borkh.) Germplasm and Their Evaluation for Scab Management Strategies.

The large genetic evolution due to the sexual reproduction-mediated gene assortments and propensities has made Venturia inaequalis (causing apple scab) unique with respect to its management strategies. The resistance in apple germplasm against the scab, being controlled for by more than fifteen genes, has limited gene alteration-based investigations. Therefore, a biological approach of bacterial endophyte community dynamics was envisioned across the apple germplasm in context to the fungistatic behavior against V. inaequalis. A total of 155 colonies of bacterial endophytes were isolated from various plant parts of the apple, comprising 19 varieties, and after screening for antifungal behavior followed by morphological, ARDRA, and sequence analysis, a total of 71 isolates were selected for this study. The alpha diversity indices were seen to fluctuate greatly among the isolation samples in context to microflora with antifungal behavior. As all the isolates were screened for the presence of various metabolites and some relevant genes that directly or indirectly influence the fungistatic behavior of the isolated microflora, a huge variation among the isolated microflora was observed. The outstanding isolates showing highest percentage growth inhibition of V. inaequalis were exploited to raise a bio-formulation, which was tested against the scab prevalence in eight apple varieties under controlled growth conditions. The formulation at all the concentrations caused considerable reductions in both the disease severity and disease incidence in all the tested apple varieties. Red Delicious being most important cultivar of the northwestern Himalayas was further investigated for its biochemical behavior in formulation and the investigation revealed different levels of enzyme production, chlorophyll, and sugars against the non-inoculated control.

Mechanisms Underlying Graft Union Formation and Rootstock Scion Interaction in Horticultural Plants.

Grafting is a common practice for vegetative propagation and trait improvement in horticultural plants. A general prerequisite for successful grafting and long term survival of grafted plants is taxonomic proximity between the root stock and scion. For the success of a grafting operation, rootstock and scion should essentially be closely related. Interaction between the rootstock and scion involves complex physiological-biochemical and molecular mechanisms. Successful graft union formation involves a series of steps viz., lining up of vascular cambium, generation of a wound healing response, callus bridge formation, followed by vascular cambium formation and subsequent formation of the secondary xylem and phloem. For grafted trees compatibility between the rootstock/scion is the most essential factor for their better performance and longevity. Graft incompatibility occurs on account of a number of factors including of unfavorable physiological responses across the graft union, transmission of virus or phytoplasma and anatomical deformities of vascular tissue at the graft junction. In order to avoid the incompatibility problems, it is important to predict the same at an early stage. Phytohormones, especially auxins regulate key events in graft union formation between the rootstock and scion, while others function to facilitate the signaling pathways. Transport of macro as well as micro molecules across long distances results in phenotypic variation shown by grafted plants, therefore grafting can be used to determine the pattern and rate of recurrence of this transport. A better understanding of rootstock scion interactions, endogenous growth substances, soil or climatic factors needs to be studied, which would facilitate efficient selection and use of rootstocks in the future. Protein, hormones, mRNA and small RNA transport across the junction is currently emerging as an important mechanism which controls the stock/scion communication and simultaneously may play a crucial role in understanding the physiology of grafting more precisely. This review provides an understanding of the physiological, biochemical and molecular basis underlying grafting with special reference to horticultural plants.