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Background Diabetes mellitus is often associated with neurohistopathological changes, resulting in cognitive deficits. This study aimed to explore the neurohistopathological alterations induced by Theobroma Cacao and Camellia Sinensis extracts in diabetic male Wistar rats. Methods In this randomized controlled trial, a total of 64 male Wistar rats aged between 8 and 12 weeks were allocated evenly into eight different groups. The first group, consisting of eight rats, served as the control, receiving only a standard diet with no additional treatment. The second group was treated with 150mg/kg body weight of alloxan to induce a diabetic model. The third group received a metformin treatment at a dose of 100mg/kg body weight. The fourth and fifth groups were administered with Theobroma cacao and Camellia sinensis extracts, respectively, at respective doses of 340 mg/kg and 200 mg/kg body weight. Groups six and seven were diabetic models treated with either Theobroma cacao extract (340 mg/kg) or Camellia sinensis extract (200 mg/kg). The eighth group, another diabetic model, was treated with a combination of both extracts at the same doses. Brain tissues were harvested at the end of an eight-week treatment period for histopathological evaluation. Cresyl violet staining was the method used for histopathological examination of the harvested brain tissues. Results Histopathological evaluations revealed normal neuronal structures in the control group. Alloxan-treated rats displayed significant neurodegeneration, including vacuolization and apoptosis. Metformin treatment showed moderate improvements in the neural architecture. Remarkably, Theobroma Cacao and Camellia Sinensis extracts exhibited protective effects against neurodegeneration in both non-diabetic and diabetic rats. Furthermore, a combination of both extracts in diabetic rats led to synergistic improvements in the neural structures, closely approximating normal conditions. One-way Analysis of Variance (ANOVA) revealed significant differences among the groups (F(7,56) = 24.11, p < 0.001). A Tukey post hoc test further indicated significant improvements in Metformin, Theobroma Cacao, and Camellia Sinensis-treated groups compared to the alloxan-induced diabetes model. Conclusions Both Theobroma Cacao and Camellia Sinensis extracts unveiled notable promise in countering the neurohistopathological alterations spurred by diabetes in the study. This pioneering observation accentuates the innovative possibility of utilizing these natural extracts as potential therapeutic agents for neural complications in diabetes mellitus. The compelling findings of this study contribute significantly to the existing body of research and emphatically advocate for further exhaustive exploration into the mechanistic actions of Theobroma Cacao and Camellia Sinensis extracts. The understanding gleaned from such in-depth studies could revolutionize the approach to managing and treating neural complications associated with diabetes, thereby enhancing the quality of life for affected individuals.
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Background Type 2 diabetes management often necessitates a multifaceted approach encompassing metabolic and anthropometric parameters. This study explores the potential of vestibular stimulation activities and yoga as complementary strategies in improving the health of individuals with type 2 diabetes. Methods A total of 180 participants were divided into three groups: vestibular exercises alone, yoga alone, and a combined group undertaking both interventions. Various metabolic parameters including fasting blood sugar (FBS), postprandial blood sugar (PPBS), HbA1c (glycosylated hemoglobin), cholesterol, lipid profile, and blood pressure, alongside anthropometric parameters like body mass index (BMI), body fat percentage, waist, hip circumference, waist-hip ratio, and arm and thigh circumference, were measured at baseline and after three months of intervention. Results Vestibular exercises and yoga, when practiced separately, demonstrated significant reductions in FBS (p < 0.01 for both). Both interventions were also effective in improving PPBS control (p < 0.01). Yoga led to a greater decrease in HbA1c compared to the control group (p < 0.01), suggesting a stronger impact on long-term glucose regulation. Vestibular exercises reduced total cholesterol and low-density lipoprotein (LDL) significantly (p < 0.01), while yoga additionally lowered triglycerides and increased high-density lipoprotein (HDL) cholesterol (p < 0.01), and notably reduced systolic blood pressure (p < 0.01). In terms of anthropometric parameters, the yoga group exhibited a significant reduction in BMI (p < 0.01), with the combined group showcasing the most substantial reduction (p < 0.01). Both yoga and the combined group achieved significant reductions in body fat percentage (p < 0.01), waist and hip circumferences (p < 0.01), and arm and thigh circumferences (p < 0.01). The combined intervention showed a borderline significant decrease in waist-hip ratio (p = 0.074). Conclusion Vestibular stimulation activities and yoga, whether practiced separately or together, have a beneficial impact on various metabolic and anthropometric parameters in individuals with type 2 diabetes. Combining these interventions appears to yield the most pronounced improvements, offering a holistic approach to enhancing type 2 diabetes management. These findings emphasize the potential of incorporating vestibular stimulation activities and yoga into diabetes care programs to promote overall health and well-being in individuals with type 2 diabetes.
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BACKGROUND: In Indian traditional medicine, the Abutilon indicum plant, colloquially known as "Country mallow" or "Thuthi", has been vouched for its efficacy in treating conditions such as bronchitis and diabetes. The study aimed to explore the chemical constituents and antioxidant strength of the ethanolic extracts derived from the leaves of this plant (ELEAI). OBJECTIVES: To qualitatively pinpoint the phytochemicals in the ethanolic extract of abutilon indicum leaves (ELEAI), utilize high-performance thin layer chromatography (HPTLC) to quantitatively analyze the identified compounds within the ELEAI, and gauge its antioxidant capability through the DPPH method, benchmarking the outcomes against the recognized standard, ascorbic acid. METHODS: Abutilon indicum leaves, originating from Telangana, were authenticated by taxonomists at Osmania University. After cleaning and drying, the leaves were powdered. A mixture of ethanol and water (70:30 ratio) was then used to extract the compounds in a Soxhlet extractor for a duration of 72 hours at a temperature of 60°C. The liquid extract was subsequently evaporated to form a light-brown powder, which was stored at 20°C under shade for later use. RESULTS: Preliminary analyses indicated that ELEAI was rich in both primary and secondary metabolites. Luteolin, a known phytochemical, was quantitatively confirmed in the extract using HPTLC. Impressively, the DPPH assay highlighted ELEAI's remarkable antioxidant capabilities. CONCLUSION: Abutilon indicum showcases notable therapeutic potential with its rich phytochemical content and strong antioxidant activity, making it a promising candidate for further pharmacological research and natural drug formulation.
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BACKGROUND: Abutilon indicum, widely found in India, Sri Lanka, and parts of America and Malaysia, is renowned for its rich bioactive compounds including alkaloids, flavonoids, and sesquiterpene lactones. Due to its diverse pharmacological potential, it has garnered significant attention in traditional medicine. In particular, the ethanolic leaf extract of Abutilon indicum (ELEAI) has demonstrated anti-inflammatory effects, notably targeting the 5-lipoxygenase enzyme pivotal in inflammatory responses. OBJECTIVE: This study aimed to elucidate the impact of the ELEAI on proinflammatory marker gene expression induced by isoniazid (INH). METHODS: A total of 36 rats were systematically divided into six experimental groups. The control group received DMSO orally for the initial 30 days followed by distilled water for the subsequent 30 days. The INH group received a daily dose of INH (30 mg/kg b.w., i.p.) for 30 days and the rats were then sacrificed on day 30. The ELEAI (250 mg/kg) group was administered INH daily for 30 days, followed by daily post-treatment with ELEAI (250 mg/kg) for another 30 days. Similarly, the ELEAI (500 mg/kg) group received INH daily for 30 days, followed by daily post-treatment with ELEAI (500 mg/kg) for another 30 days. The silymarin (SIL) group was given INH daily for 30 days, followed by post-treatment with SIL at a dose of 100 mg/kg body weight daily for the subsequent 30 days. Finally, the ELEAI (500 mg/kg) alone group was administered distilled water orally for the first 30 days and then received ELEAI at a dose of 500 mg/kg b.w. orally once daily for the next 30 days. RESULTS: Continuous INH exposure for a month led to a pronounced increase in proinflammatory genes like TNF-α, TGF-ß, and NF-kB and a decrease in the IkB gene in rat liver tissues. Subsequent treatment with SIL (100 mg/kg) and ELEAI (250 and 500 mg/kg) post-INH exposure resulted in a marked decrease in proinflammatory genes and a surge in IkB expression. CONCLUSION: The findings suggest that the ELEAI exerts a dose-responsive influence on proinflammatory activities. Notably, A. indicum counteracts inflammation, especially that triggered by bradykinin and prostaglandins. The ELEAI showcases promising therapeutic potential, exhibiting both pro and anti-inflammatory properties and antiproliferative characteristics.
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BACKGROUND: Carica papaya seeds are rich in phytochemicals with potential health benefits, warranting safety and antioxidant assessments. This study comprehensively examined the ethanolic extract of Carica papaya seeds (EECPS) to elucidate its phytochemical composition, acute toxicity profile, and antioxidant activity. METHODS: Phytochemical analysis of EECPS revealed the presence of various bioactive compounds, including flavonoids, tannins, phenols, alkaloids, proteins, glycosides, and saponins. Additionally, the presence of sulfuric acid was confirmed. Acute toxicity assessment involved oral administration of EECPS at 2000mg/kg body weight to Wistar rats, with a 14-day observation period. General parameters, body weight changes, and histopathological examination of kidney and liver tissues were evaluated. Antioxidant activity was assessed using the 2,2-Diphenyl-1-picrylhydrazyl (DPPH) assay, and the half-maximal inhibitory concentration (IC50) value of EECPS was compared to that of gallic acid. RESULTS: Phytochemical analysis confirmed the diverse composition of EECPS, suggesting its potential health benefits and biological activity. Acute toxicity assessment revealed no adverse effects, with rats exhibiting normal behavior, weight stability, and no histopathological abnormalities in vital organs. The gallic acid IC50 value was determined to be 5.73±0.02 µg/mL, indicating its antioxidant potency. EECPS exhibited antioxidant properties in a dose-dependent manner, with higher concentrations demonstrating increased DPPH free radical quenching capacity. The IC50 value for EECPS was calculated from the dose-response curve to be 39.41±1.61 µg/mL (expressed as mean ± standard error of the mean (SEM)). CONCLUSION: The phytochemical analysis of EECPS highlights its diverse composition and potential health benefits. Acute toxicity studies in rats confirm its safety for oral administration, with no adverse effects observed. EECPS exhibits significant antioxidant activity, as indicated by its IC50 value. These findings suggest that EECPS holds promise for therapeutic use and health applications. However, further research is needed to determine its precise antioxidant potential. Subchronic and chronic toxicity studies are recommended to establish its safety profile definitively and unlock its full potential for healthcare and nutrition.
