Bioacoustic characterization of the black-and-white ruffed lemur (Varecia variegata) vocal repertoire.

Ruffed lemurs (Varecia spp.) exhibit a unique suite of behavioral traits compared to other lemur species, which includes their fluid fission-fusion social dynamics, communal rearing of parked litters, and pronounced frugivory in their humid rainforest habitats. Given these traits, and the dense rainforests they inhabit, vocal communication may be key to maintaining social cohesion, coordinating infant care, and/or defending their high-quality food resources. Indeed, they are known for their raucous 'roar-shriek' calls. However, there has been surprisingly little research on vocal communication in Varecia species and only two previously published repertoires, both of which were qualitative descriptions of their calls. In this study, we quantitatively examined the vocal repertoire of wild black-and-white ruffed lemurs (Varecia variegata) at Mangevo, Ranomafana National Park, Madagascar. We characterized 11 call types using 33 bioacoustic parameters related to frequency, duration, tonality, and composition. We also used discriminant function analysis and hierarchical clustering to quantitatively and objectively classify call types within the black-and-white ruffed lemur vocal repertoire. The repertoire consists of both monosyllabic and multisyllabic calls that are individually given or emitted in contagious choruses. Eight of the 11 calls were also used in combination or in larger multi-call sequences. The discriminant function analysis correctly assigned call types with 87% success, though this varied greatly by call type (1-65%). Hierarchical clustering identified 3-4 robust clusters, indicating low clustering structure in the data and suggesting that V. variegata exhibits a graded vocal repertoire. Future work should consider the environmental and behavioral contexts in which calls are used to better understand the function of these call types and combinations.

Factors affecting call usage in wild black-and-white ruffed lemurs (Varecia variegata) at Mangevo, Ranomafana National Park.

Vocal communication is an important modality for group-living primates inhabiting dense forest habitats that can hinder visual and olfactory signals. Nevertheless, research on primate vocalizations has historically focused on a narrow subset of haplorhine taxa; comparatively few studies have been focused on strepsirrhines, despite facing similar ecological and social challenges. Ruffed lemurs (Varecia)-a taxon known for their raucous calls-are rainforest specialists that exhibit strong fission-fusion dynamics and communally rear large litters of young. However, surprisingly few studies have examined Varecia vocalizations in the wild, meaning virtually nothing is known about the call types or how they facilitate their unique social and reproductive strategies. Our goal for this study was to examine how various contextual factors such as weather, behavioral state, and subgroup size and composition affected vocal activity across call types in wild black-and-white ruffed lemurs. We conducted focal follows on 31 individuals (two communities) in Mangevo (Ranomafana National Park) from May-August 2019 to record behavioral and vocal activity. We distinguished 11 call types, although three (hum, roar-shriek, chatter) constituted the majority of vocal activity. Calling rates were consistent throughout the day, but decreased with high rainfall. We found sex- and subgroup-specific differences in call usage, likely related to female dominance and subgroup composition, respectively. We identified behavioral contexts that some call types were consistently given in; this can be used to help infer call function. This study provides some of the first quantitative analyses of ruffed lemur vocal communication and lays the groundwork for more systematic hypothesis testing in future studies.

Can colour vision re-evolve? Variation in the X-linked opsin locus of cathemeral Azara's owl monkeys (Aotus azarae azarae).

BACKGROUND: Do evolutionary specializations lead to evolutionary constraint? This appears plausible, particularly when specialization leads to loss of complex adaptations. In the owl monkey lineage, nocturnality clearly arose from a diurnal ancestor. This behavioural shift was accompanied by morphological changes in the eye and orbit and complete loss of colour vision via missense mutations in the gene encoding the short-wave sensitive visual pigment (SWS opsin). Interestingly, at least one subspecies of owl monkey, Azara's owl monkey (Aotus azarae azarae), has regained activity in daylight. Given that all primate species that are active in daylight, including primarily diurnal species and species that are active during both day and night, have at least dichromatic colour vision, it seems reasonable to propose that dichromacy would be adaptive in A. a. azarae. With a disabled SWS opsin, the main avenue available for Azara's owl monkeys to re-evolve colour vision is via a polymorphism in the intact X-linked opsin locus, which commonly occurs in other New World monkeys. To examine this possibility we assayed variation in the X-linked opsin of A. a. azarae, focusing on the three exons (3, 4 and 5) that control spectral sensitivity. RESULTS: We found low opsin genetic variation on a population level, and no differences at the three main sites that lead to variation in spectral sensitivity in the opsins of other New World monkeys. Two rare alleles with single amino acid variants are segregating in the population, but previous functional studies indicate that these are unlikely to affect spectral sensitivity. CONCLUSIONS: Genetic constraint on the re-evolution of colour vision is likely operating in Azara's owl monkey, which may affect the niche that this subspecies is able to occupy.

Chewing variation in lepidosaurs and primates.

Mammals chew more rhythmically than lepidosaurs. The research presented here evaluated possible reasons for this difference in relation to differences between lepidosaurs and mammals in sensorimotor systems. Variance in the absolute and relative durations of the phases of the gape cycle was calculated from kinematic data from four species of primates and eight species of lepidosaurs. The primates exhibit less variance in the duration of the gape cycle than in the durations of the four phases making up the gape cycle. This suggests that increases in the durations of some gape cycle phases are accompanied by decreases in others. Similar effects are much less pronounced in the lepidosaurs. In addition, the primates show isometric changes in gape cycle phase durations, i.e. the relative durations of the phases of the gape cycle change little with increasing cycle time. In contrast, in the lepidosaurs variance in total gape cycle duration is associated with increases in the proportion of the cycle made up by the slow open phase. We hypothesize that in mammals the central nervous system includes a representation of the optimal chew cycle duration maintained using afferent feedback about the ongoing state of the chew cycle. The differences between lepidosaurs and primates do not lie in the nature of the sensory information collected and its feedback to the feeding system, but rather the processing of that information by the CNS and its use feed-forward for modulating jaw movements and gape cycle phase durations during chewing.

Measurement of the lifetime of the Bc+/- meson in the semileptonic decay channel.

Using approximately 1.3 fb(-1) of data collected by the D0 detector between 2002 and 2006, we measure the lifetime of the Bc+/- meson in the Bc-/+-->J/psimicro+/-+X final state. A simultaneous unbinned likelihood fit to the J/psi+micro invariant mass and lifetime distributions yields a signal of 881+/-80(stat) candidates and a lifetime measurement of tau(Bc+/-)=0.448(-0.036)(+0.038)(stat)+/-0.032(syst) ps.

Measurement of the semileptonic branching ratio of B_{s};{0} to an orbitally excited D_{s};{**} state: Br(B_{s};{0}-->D_{s1};{-}(2536)mu;{+}nuX).

In a data sample of approximately 1.3 fb;{-1} collected with the D0 detector between 2002 and 2006, the orbitally excited charm state D_{s1};{+/-}(2536) has been observed with a measured mass of 2535.7+/-0.6(stat)+/-0.5(syst) MeV/c;{2} via the decay mode B_{s};{0}-->D_{s1};{-}(2536)mu;{+}nu_{mu}X. A first measurement is made of the branching ratio product Br(b[over ]-->D_{s1};{-}(2536)mu;{+}nu_{mu}X)xBr(D_{s1};{-}-->D;{*-}K_{S};{0}). Assuming that D_{s1};{-}(2536) production in semileptonic decay is entirely from B_{s};{0}, an extraction of the semileptonic branching ratio Br(B_{s};{0}-->D_{s1};{-}(2536)mu;{+}nu_{mu}X) is made.

Measurement of the forward-backward charge asymmetry and extraction of sin2thetaWeff in pp-->Z/gamma* + X-->e+e- +X events produced at sqrt[s] = 1.96 TeV.

We present a measurement of the forward-backward charge asymmetry (A FB) in pp-->Z/gamma* + X-->e+e(-) + X events at a center-of-mass energy of 1.96 TeV using 1.1 fb(-1) of data collected with the D0 detector at the Fermilab Tevatron collider. A FB is measured as a function of the invariant mass of the electron-positron pair, and found to be consistent with the standard model prediction. We use the A FB measurement to extract the effective weak mixing angle sin2thetaWeff = 0.2326+/-0.0018(stat)+/-0.0006(syst).

Search for neutral Higgs bosons in multi-b-jet events in pp[over] collisions at sqrt[s]=1.96 TeV.

Data recorded by the D0 experiment at the Fermilab Tevatron Collider are analyzed to search for neutral Higgs bosons produced in association with b quarks. This production mode can be enhanced in the minimal supersymmetric standard model (MSSM). The search is performed in the three b quark channel using multijet triggered events corresponding to an integrated luminosity of 1 fb(-1). No statistically significant excess of events with respect to the predicted background is observed and limits are set in the MSSM parameter space.

Measurement of Bs0 mixing parameters from the flavor-tagged decay Bs0-->J/psiphi.

From an analysis of the flavor-tagged decay Bs0-->J/psiphi we obtain the width difference between the Bs0 light and heavy mass eigenstates, DeltaGammas = 0.19+/-0.07(stat)(-0.01)+0.02(syst) ps(-1), and the CP-violating phase, phi s= -0.57(-0.30)+0.24(stat)(-0.02)+0.08(syst). The allowed 90% CL intervals of DeltaGammas and phi s are 0.06 < DeltaGammas < 0.30 ps(-1) and -1.20 < phi s < 0.06, respectively. The data sample corresponds to an integrated luminosity of 2.8 fb(-1) accumulated with the D0 detector at the Fermilab Tevatron collider.

Measurement of the polarization of the upsilon(1S) and upsilon(2S) states in pp collisions at square root[s]=1.96 TeV.

We present a study of the polarization of the Upsilon(1S) and Upsilon(2S) states using a 1.3 fb;{-1} data sample collected by the D0 experiment in 2002-2006 during run II of the Fermilab Tevatron Collider. We measure the polarization parameter alpha=(sigma_{T}-2sigma_{L})/(sigma_{T}+2sigma_{L}), where sigma_{T} and sigma_{L} are the transversely and longitudinally polarized components of the production cross section, as a function of the transverse momentum (p_{T};{Upsilon}) for the Upsilon(1S) and Upsilon(2S). Significant p_{T};{Upsilon}-dependent longitudinal polarization is observed for the Upsilon(1S). A comparison with theoretical models is presented.

Search for large extra dimensions via single photon plus missing energy final states at sqrt s = 1.96 TeV.

We report on a search for large extra dimensions in a data sample of approximately 1 fb(-1) of pp[over] collisions at sqrt s=1.96 TeV. We investigate Kaluza-Klein graviton production with a photon and missing transverse energy in the final state. At the 95% C.L. we set limits on the fundamental mass scale M(D) from 884 to 778 GeV for two to eight extra dimensions.

Observation of the Bc meson in the exclusive decay Bc-->J/psipi.

A fully reconstructed Bc-->J/psipi signal is observed with the D0 detector at the Fermilab Tevatron pp[over] collider using 1.3 fb(-1) of integrated luminosity. The signal consists of 54+/-12 candidates with a significance that exceeds 5 standard deviations, and confirms earlier observations of this decay. The measured mass of the Bc meson is 6300+/-14(stat)+/-5(syst) MeV/c2.

Search for decay of a fermiophobic Higgs boson hf-->gammagamma with the D0 detector at sqrt s=1.96 TeV.

We report the results of a search for a narrow resonance decaying into two photons in 1.1 fb;{-1} of data collected by the D0 experiment at the Fermilab Tevatron Collider during the period 2002-2006. We find no evidence for such a resonance and set a lower limit on the mass of a fermiophobic Higgs boson of m_{h_{f}}>100 GeV at the 95% C.L. This exclusion limit exceeds those obtained in previous searches at the Fermilab Tevatron and covers a significant region of the parameter space B(h_{f}-->gammagamma) vs m_{h_{f}} which was not accessible at the CERN Large Electron-Positron Collider.

Measurement of the inclusive jet cross section in pp[over] collisions at square root [s]=1.96 TeV.

We report on a measurement of the inclusive jet cross section in pp[over ] collisions at a center-of-mass energy sqrt[s]=1.96 TeV using data collected by the D0 experiment at the Fermilab Tevatron Collider corresponding to an integrated luminosity of 0.70 fb;{-1}. The data cover jet transverse momenta from 50 to 600 GeV and jet rapidities in the range -2.4 to 2.4. Detailed studies of correlations between systematic uncertainties in transverse momentum and rapidity are presented, and the cross section measurements are found to be in good agreement with next-to-leading order QCD calculations.

Search for pair production of doubly charged Higgs bosons in the H++H- - -->mu+ mu+ mu- mu- final state.

We report the results of a search for pair production of doubly charged Higgs bosons via pp over-->H++H - - X-->mu+ mu+ mu- mu- X at sqrt s=1.96 TeV. We use a data set corresponding to an integrated luminosity of 1.1 fb(-1) collected from 2002 to 2006 by the D0 detector at the Fermilab Tevatron Collider. In the absence of an excess above the standard model background, lower mass limits of M(H L +/- +/-) >150 GeV/c2 and M(H R+/- +/-) >127 GeV/c2 at 95% C.L. are set, respectively, for left-handed and right-handed doubly charged Higgs bosons assuming a 100% branching ratio into muons.

Search for Higgs bosons decaying to tau pairs in pp over collisions with the D0 detector.

We present a search for the production of neutral Higgs bosons varphi decaying into tau+tau - final states in pp over collisions at a center-of-mass energy of 1.96 TeV. The data, corresponding to an integrated luminosity of approximately 1 fb(-1), were collected by the D0 experiment at the Fermilab Tevatron Collider. Limits on the production cross section times branching ratio are set. The results are interpreted in the minimal supersymmetric standard model yielding limits that are the most stringent to date at hadron colliders.

Search for scalar neutrino superpartners in e+mu final states in pp collisions at sqrt[s]=1.96 TeV.

We report a search for R-parity-violating production and decay of sneutrino particles in the emu final state with 1.04+/-0.06 fb-1 of data collected with the D0 detector at the Fermilab Tevatron Collider in 2002-2006. Good agreement between the data and the standard model prediction is observed. With no evidence for new physics, we set limits on the R-parity-violating couplings lambda'311 and lambda312 as a function of the sneutrino mass.

First study of the radiation-amplitude zero in Wgamma production and limits on anomalous WWgamma couplings at sqrt[s]=1.96 TeV.

We present results from a study of pp-->Wgamma+X events utilizing data corresponding to 0.7 fb{-1} of integrated luminosity at sqrt[s]=1.96 TeV collected by the D0 detector at the Fermilab Tevatron Collider. We set limits on anomalous WWgamma couplings at the 95% C.L. The one-dimensional 95% C.L. limits are 0.49

Search for ZZ and Zgamma* production in pp[over ] collisions at square root s=1.96 TeV and limits on anomalous ZZZ and ZZgamma* couplings.

We present a study of micro micro micro micro, eeee, and micro micro ee events using 1 fb(-1) of data collected with the D0 detector at the Fermilab Tevatron pp[over ] Collider at square root s=1.96 TeV. Requiring the lepton pair masses to be greater than 30 GeV, we observe one event, consistent with the expected background of 0.13+/-0.03 events and with the predicted standard model ZZ and Zgamma* production of 1.71+/-0.15 events. We set an upper limit on the ZZ and Zgamma* cross section of 4.4 pb at the 95% C.L. We also derive limits on anomalous neutral trilinear ZZZ and ZZgamma* gauge couplings. The one-parameter 95% C.L. coupling limits with a form-factor scale Lambda=1.2 TeV are -0.28

Measurement of the forward-backward charge asymmetry in top-quark pair production.

We present the first measurement of the integrated forward-backward charge asymmetry in top-quark-top-antiquark pair (tt) production in proton-antiproton (pp) collisions in the lepton+jets final state. Using a b-jet tagging algorithm and kinematic reconstruction assuming tt + X production and decay, a sample of 0.9 fb(-1) of data, collected by the D0 experiment at the Fermilab Tevatron Collider, is used to measure the asymmetry for different jet multiplicities. The result is also used to set upper limits on tt+X production via a Z' resonance.