RESUMEN
The phytochemical investigation of the hydromethanolic extract of Carica papaya Linn. leaves (Caricaceae) resulted in the isolation and characterization of ten compounds, namely; carpaine (1), methyl gallate (2), loliolide (3), rutin (4), clitorin (5), kaempferol-3-O-neohesperidoside (6), isoquercetin (7), nicotiflorin (8) and isorhamnetin-3-O-ß-d-glucopyranoside (9). The compounds 2, 3, 5-7 and 9 were isolated for the first time from the genus Carica. An in vitro breast cancer cytotoxicity study was evaluated with an MCF-7 cell line using the MTT assay. Methyl gallate and clitorin demonstrated the most potent cytotoxic activities with an IC50 of 1.11 ± 0.06 and 2.47 ± 0.14 µM, respectively. Moreover, methyl gallate and nicotiflorin exhibited potential EGFRwt kinase inhibition activities with an IC50 of 37.3 ± 1.9 and 41.08 ± 2.1 nM, respectively, compared with the positive control erlotinib (IC50 = 35.94 ± 1.8 nM). On the other hand, clitorin and nicotiflorin displayed the strongest aromatase kinase inhibition activities with an IC50 of 77.41 ± 4.53 and 92.84 ± 5.44 nM, respectively. Clitorin was comparable to the efficacy of the standard drug letrozole (IC50 = 77.72 ± 4.55). Additionally, molecular docking simulations of the isolated compounds to EGFR and human placental aromatase cytochrome P450 (CYP19A1) were evaluated. Methyl gallate linked with the EGFR receptor through hydrogen bonding with a pose score of -4.5287 kcal mol-1 and RMSD value of 1.69 Å. Clitorin showed the strongest interaction with aromatase (CYP19A1) for the breast cancer receptor with a posing score of -14.2074 and RMSD value of 1.56 Å. Compounds (1-3) possessed a good bioavailability score with a 0.55 value.
RESUMEN
This work presents a loop terminated asymmetric Mach-Zehnder interferometer (LT-aMZI) structure based on the widespread silicon-on-insulator (SOI) platform. Four different path length differences of the LT-aMZI, which correspond to free spectral ranges (FSR) from 0.8 to 6.4 nm, are designed. These designs are compared to the common asymmetric Mach-Zehnder interferometer (C-aMZI) and are shown to be more compact. These devices are suitable for optical filtering as well as wavelength demultiplexing (WDM) applications. A compact analytical model is derived that accurately describe the operation of the LT-MZI devices. The designs are then fabricated using Electron Beam Lithography (EBL) and characterized. The experimental data show good agreement when compared to the simulation results. To our knowledge, this is the first time LT-aMZI fabrication and characterization. Moreover, the LT-MZI spectrum can be tuned not only by the interferometer arms phase difference like C-MZI, but also by using its directional couplers coefficients, forming a spectral tunable filter. Finally, we determine the performance parameters of optical sensors and modulators and show that our proposed LT-MZI structure will enhance the sensor figure of merit (FOM) and modulator speed, power consumption and Vπ × L compared to C-MZI. A comparison between symmetric and asymmetric MZI sensors and the advantage of the latter is also mentioned.
RESUMEN
This experiment was conducted to study the effects of paulownia leaf meal (PLM) as a nontraditional feed on the growth, carcasses, digestibility, blood chemistry, and intestinal microbiota of growing rabbits. Sixty rabbits (5-weeks old) were randomly allotted to three dietary treatments containing three amounts of PLM (0%, 15%, and 30%). The results showed that PLM has a higher content of ether extract, organic matter, methionine, tyrosine, histidine, manganese, and zinc than alfalfa hay. Body weight gain decreased when 30% PLM was provided. The best feed conversion ratio was recorded in the rabbits fed 15% PLM. A notable increase in high-density lipoprotein levels with a significant decrease in low-density lipoprotein was noted in the rabbits fed the PLM diets. Total fungi and Enterobacteriaceae and total bacterial count in the feed were significantly reduced because of PLM. In the cecum, coliforms, Enterobacteriaceae species, and total bacterial count declined in the rabbits fed the PLM diets. Conclusively, up to 15% PLM can be used in rabbit diets without any deleterious effects on the performance, nutrient digestibility, and blood constituents. In addition, dietary inclusion of PLM has the potential to reduce cecal pathogenic bacteria in rabbits.
RESUMEN
The present study was carried out to investigate the toxic effects of dietary inclusion of raw Jatropha cucas meal [RJM, 3.5%] on productive and reproductive performances of laying Japanese quail and the influence of physical treatment of jatropha meal by heating at [100 oc] for 24 or 48â¯h [JH24 or JH48 respectively] on reducing these effects. The inclusion of RJM in quail diets significantly decreased the egg production, fertility and hatchability percentages and resulted in a high mortality rate. While heat-treated jatropha meal [JH24 or JH48] improved such undesirable effects. The levels of hepatic proteins related to lipid synthesis, cholesterol metabolism and those related to lipid synthesis and transporting to egg yolk were increased in JH24 and JH48 groups than RJM group. Hepatic contents of vitellogenin [VGT-II] and estrogen receptor α [ERα] and circulating estradiol [E2] were improved in JH48 than RJM and JH24 groups however still under the control values. On the other hand, estrogen receptor ß [ERß] were restored to normal control value in both JH24 and JH48 groups. Histopathological investigation revealed that RJM produced various alterations in the histoarchitecture of liver, ovary and oviducts but these alterations were reduced in both JH24 and JH48 groups. From the obtained data we concluded that heating of jatropha meal for 24â¯h improved its nutritive value and increasing the time of heating is preferable for reduction of the most of toxic impacts indicating that heat treatment can convert toxic jatropha meal to an alternative protein source for livestock feed in a cheap and clean way without combining any other types of treatments.
