Mycoremediation of azo dyes using Cyberlindnera fabianii yeast strain: Application of designs of experiments for decolorization optimization.

This study investigated the dye decolorization capacity of three yeast strains. Cyberlindnera fabianii was shortlisted for its high decolorization capacity and was further tested on various azo dyes. Based on the color of the biomass, and the UV-Vis analysis, Acid Red 14 was selected as a model dye, to examine the enzymatic biodegradation. The results showed significant increase in the intracellular and extracellular activities of laccase, tyrosinase, manganese peroxidase, and azoreductase. Phytotoxicity assessment indicated that the AR14 biodegradation by-products were not phytotoxic compared to the original dye molecules. Regarding the decolorization optimization, the screening of factors using the Plackett-Burman design showed that pH, dye concentration, and shaking speed had significant effects. These factors and their combined effect were evaluated using response surface methodology with the Box-Behnken model. The pH was the most significant factor, followed by dye concentration. The analysis of the contour plot and the 3D response surface diagram showed that the decolorization was inversely proportional to the increase in the initial dye concentration, but proportional to the initial pH and shaking speed. At optimal conditions (pH = 5.154, AR14 = 50 mg/L), C. fabianii could decolorize more than 97% of AR14 within 12 hr. PRACTITIONER POINTS: Cyberlindnera fabianii is a successful candidate for dye mycoremediation. Oxidase and reductase are the key enzymes involved in the biodegradation of azo dyes. By-products of Acid red 14 biodegradation are not phytoxic compared to the original dye. Design of experience tools enables to determine optimum conditions for efficient decolorization.

Mechanisms of hexavalent chromium resistance and removal by microorganisms.

Chromium has been and is extensively used worldwide in multiple industrial processes and is routinely discharged to the environment from such processes. Therefore, this heavy metal is a potential threat to the environment and to public health, primarily because it is non-biodegradable and environmentally persistent. Chromium exists in several oxidation states, the most stable of which are trivalent Cr(Ill) and hexavalent Cr(VI) species. Each species possesses its own individual chemical characteristics and produces its own biological effects. For example, Cr (Ill) is an essential oligoelement for humans, whereas Cr(VI) is carcinogenic and mutagenic. Several chemical methods are used to remove Cr(VI) from contaminated sites. Each of these methods has advantages and disadvantages. Currently, bioremediation is often the preferred method to deal with Cr contaminated sites, because it is eco-friendly, cost-effective and is a "natural" technology. Many yeast, bacterial and fungal species have been assessed for their suitability to reduce or remove Cr(VI) contamination. The mechanisms by which these microorganisms resist and reduce Cr(VI) are variable and are species dependent. There are several Cr-resistance mechanisms that are displayed by microorganisms. These include active efflux of Cr compounds, metabolic reduction of Cr(VI) to Cr (ill), and either intercellular or extracellular prec1p1tation. Microbial Cr (VI) removal typically involves three stages: binding of chromium to the cell surface, translocation of chromium into the cell, and reduction of Cr(VI) to Cr (ill). Cr(VI) reduction by microorganisms may proceed on the cell surface, outside the cell, or intracellularly, either directly via chromate reductase enzymes, or indirectly via metabolite reduction of Cr(VI). The uptake of chromium ions is a biphasic process. The primary step is known as biosorption, a metabolic energyindependent process. Thereafter, bioaccumulation occurs, but is much slower, and is dependent on cell metabolic activity. Choosing an appropriate bioremediation strategy for Cr is extremely important and must involve investigating and understanding the key mechanisms that are involved in microbial resistance to and removal of Cr(VI).

Hexavalent chromium removal by a novel Serratia proteamaculans isolated from the bank of Sebou River (Morocco).

The novel Serratia proteamaculans isolated from a chromium-contaminated site was tolerant to a concentration of 500 mg Cr(VI)/l. The optimum pH and temperature for reduction of Cr(VI) by S. proteamaculans were found to be 7.0 and 30 °C, respectively. The Cr(VI) reduction rate decreased with the increase in Cr(VI) concentration from 100 to 400 mg/l, suggesting the enzymatic chromium reduction. Resting and permeabilised cell assays provided the better evidence that chromate reduction in S. proteamaculans is enzymatic. Reduction by cell-free filtrate shows no extracellular chromate-reducing activity, revealing that this activity may be associated to membrane fraction and/or cytosolic fraction. Assays conducted with cytosolic and particulate fraction of S. proteamaculans confirmed the role of membrane-bound proteins in Cr(VI) reduction. Furthermore, chromium reduced by heat-treated cells suggests that membrane-associated chromate reductase activity of S. proteamaculans is preceded by its adsorption on the cell surface.