RESUMEN
Fluorescently labeled multimeric complexes of peptide-MHC, the molecular entities recognized by the T cell receptor, have become essential reagents for detection of antigen-specific CD8(+) T cells by flow cytometry. Here we present a method for high-throughput parallel detection of antigen-specific T cells by combinatorial encoding of MHC multimers. Peptide-MHC complexes are produced by UV-mediated MHC peptide exchange and multimerized in the form of streptavidin-fluorochrome conjugates. Eight different fluorochromes are used for the generation of MHC multimers and, by a two-dimensional combinatorial matrix, these eight fluorochromes are combined to generate 28 unique two-color codes. By the use of combinatorial encoding, a large number of different T cell populations can be detected in a single sample. The method can be used for T cell epitope mapping, and also for the monitoring of CD8(+) immune responses during cancer and infectious disease or after immunotherapy. One panel of 28 combinatorially encoded MHC multimers can be prepared in 4 h. Staining and detection takes a further 3 h.
Asunto(s)
Linfocitos T CD8-positivos/inmunología , Mapeo Epitopo/métodos , Epítopos de Linfocito T/química , Antígenos H-2/metabolismo , Antígenos HLA/metabolismo , Animales , Antígenos/química , Linfocitos T CD8-positivos/química , Colorantes Fluorescentes/análisis , Humanos , Ratones , Imagen Óptica/métodos , Puntos CuánticosRESUMEN
Autoreactive cytotoxic CD8 T-cells (CTLs) play a key pathogenic role in the destruction of insulin-producing beta-cells resulting in type 1 diabetes. However, knowledge regarding their targets is limited, restricting the ability to monitor the course of the disease and immune interventions. In a multi-step discovery process to identify novel CTL epitopes in human preproinsulin (PPI), PPI was digested with purified human proteasomes, and resulting COOH-fragments aligned with algorithm-predicted HLA-binding peptides to yield nine potential HLA-A1, -A2, -A3 or -B7-restricted candidates. An UV-exchange method allowed the generation of a repertoire of multimers including low-affinity HLA-binding peptides. These were labeled with quantum dot-fluorochromes and encoded in a combinatorial fashion, allowing parallel and sensitive detection of specific, low-avidity T-cells. Significantly increased frequencies of T-cells against four novel PPI epitopes (PPI(4-13)/B7, PPI(29-38)/A2, PPI(76-84)/A3 and PPI(79-88)/A3) were detected in stored blood of patients with recent onset diabetes but not in controls. Changes in frequencies of circulating CD8 T-cells against these novel epitopes were detected in blood of islet graft recipients at different time points after transplantation, which correlated with clinical outcome. In conclusion, our novel strategy involving a sensitive multiplex detection technology and requiring minimal volumes of stored blood represents a major improvement in the direct ex-vivo characterization and enumeration of immune cells in the pathogenesis of type 1 diabetes.
Asunto(s)
Autoinmunidad , Linfocitos T CD8-positivos/metabolismo , Técnicas Químicas Combinatorias , Diabetes Mellitus Tipo 1/inmunología , Células Secretoras de Insulina/metabolismo , Insulina/química , Péptidos/química , Precursores de Proteínas/química , Algoritmos , Secuencia de Aminoácidos , Linfocitos T CD8-positivos/inmunología , Linfocitos T CD8-positivos/patología , Diabetes Mellitus Tipo 1/diagnóstico , Diabetes Mellitus Tipo 1/metabolismo , Diabetes Mellitus Tipo 1/patología , Epítopos , Epítopos de Linfocito T/química , Epítopos de Linfocito T/inmunología , Epítopos de Linfocito T/metabolismo , Rechazo de Injerto/diagnóstico , Rechazo de Injerto/inmunología , Rechazo de Injerto/metabolismo , Antígenos HLA-A/química , Antígenos HLA-A/inmunología , Antígenos HLA-A/metabolismo , Antígeno HLA-B7/química , Antígeno HLA-B7/inmunología , Antígeno HLA-B7/metabolismo , Humanos , Insulina/inmunología , Insulina/metabolismo , Células Secretoras de Insulina/inmunología , Células Secretoras de Insulina/patología , Células Secretoras de Insulina/trasplante , Trasplante de Islotes Pancreáticos/inmunología , Complejo Mayor de Histocompatibilidad , Datos de Secuencia Molecular , Péptidos/análisis , Péptidos/inmunología , Péptidos/metabolismo , Complejo de la Endopetidasa Proteasomal/metabolismo , Unión Proteica , Precursores de Proteínas/inmunología , Precursores de Proteínas/metabolismo , Puntos CuánticosRESUMEN
OBJECTIVE: Type 1 diabetes results from selective T-cell-mediated destruction of the insulin-producing beta-cells in the pancreas. In this process, islet epitope-specific CD8(+) T-cells play a pivotal role. Thus, monitoring of multiple islet-specific CD8(+) T-cells may prove to be valuable for measuring disease activity, progression, and intervention. Yet, conventional detection techniques (ELISPOT and HLA tetramers) require many cells and are relatively insensitive. RESEARCH DESIGN AND METHODS: Here, we used a combinatorial quantum dot major histocompatibility complex multimer technique to simultaneously monitor the presence of HLA-A2 restricted insulin B(10-18), prepro-insulin (PPI)(15-24), islet antigen (IA)-2(797-805), GAD65(114-123), islet-specific glucose-6-phosphatase catalytic subunit-related protein (IGRP)(265-273), and prepro islet amyloid polypeptide (ppIAPP)(5-13)-specific CD8(+) T-cells in recent-onset diabetic patients, their siblings, healthy control subjects, and islet cell transplantation recipients. RESULTS: Using this kit, islet autoreactive CD8(+) T-cells recognizing insulin B(10-18), IA-2(797-805), and IGRP(265-273) were shown to be frequently detectable in recent-onset diabetic patients but rarely in healthy control subjects; PPI(15-24) proved to be the most sensitive epitope. Applying the "Diab-Q-kit" to samples of islet cell transplantation recipients allowed detection of changes of autoreactive T-cell frequencies against multiple islet cell-derived epitopes that were associated with disease activity and correlated with clinical outcome. CONCLUSIONS: A kit was developed that allows simultaneous detection of CD8(+) T-cells reactive to multiple HLA-A2-restricted beta-cell epitopes requiring limited amounts of blood, without a need for in vitro culture, that is applicable on stored blood samples.
Asunto(s)
Linfocitos T CD8-positivos/inmunología , Diabetes Mellitus Tipo 1/inmunología , Epítopos/inmunología , Trasplante de Islotes Pancreáticos/inmunología , Islotes Pancreáticos/inmunología , Complejo Mayor de Histocompatibilidad/inmunología , Adolescente , Niño , Preescolar , Femenino , Citometría de Flujo , Antígenos HLA-A/inmunología , Humanos , Lactante , Células Secretoras de Insulina/inmunología , Masculino , Estadísticas no ParamétricasRESUMEN
The use of fluorescently labeled major histocompatibility complex multimers has become an essential technique for analyzing disease- and therapy-induced T-cell immunity. Whereas classical major histocompatibility complex multimer analyses are well-suited for the detection of immune responses to a few epitopes, limitations on human-subject sample size preclude a comprehensive analysis of T-cell immunity. To address this issue, we developed a combinatorial encoding strategy that allows the parallel detection of a multitude of different T-cell populations in a single sample. Detection of T cells from peripheral blood by combinatorial encoding is as efficient as detection with conventionally labeled multimers but results in a substantially increased sensitivity and, most notably, allows comprehensive screens to be performed. We obtained proof of principle for the feasibility of large-scale screening of human material by analysis of human leukocyte antigen A3-restricted T-cell responses to known and potential melanoma-associated antigens in peripheral blood from individuals with melanoma.
Asunto(s)
Antígenos de Histocompatibilidad/metabolismo , Subgrupos de Linfocitos T/inmunología , Antígenos , Antígenos de Neoplasias , Separación Celular/métodos , Epítopos , Colorantes Fluorescentes , Antígenos de Histocompatibilidad/química , Humanos , Técnicas Inmunológicas , Antígenos Específicos del Melanoma , Nanotecnología , Proteínas de Neoplasias , Péptidos/inmunología , Estructura Cuaternaria de Proteína , Puntos Cuánticos , Sensibilidad y Especificidad , Subgrupos de Linfocitos T/clasificación , Subgrupos de Linfocitos T/citologíaRESUMEN
Recombinant major histocompatibility complex (MHC) class I molecules complexed with pathogen-specific or other disease-associated antigens have become essential reagents for the analysis of adaptive T-cell responses. However, conventional techniques for the production of recombinant peptide-MHC (pMHC) complexes are highly involved and thereby limit the use of pMHC complexes in terms of antigen diversity. To make pMHC-based techniques suitable for high-throughput analyses we developed an MHC peptide exchange technology based on the use of conditional MHC ligands. This technology enables the parallel production of thousands of different pMHC complexes within hours, allowing the development of high-throughput MHC-based assay systems to identify MHC ligands and cytotoxic T-cell responses. These high-throughput assays should prove valuable for the screening of entire disease-associated proteomes, including pathogen-encoded proteomes, tumor-associated antigens, and autoimmune antigens.
Asunto(s)
Mapeo Epitopo/métodos , Epítopos de Linfocito T/inmunología , Genes MHC Clase I , Péptidos/inmunología , Biotinilación , Linfocitos T CD8-positivos/inmunología , Mapeo Epitopo/economía , Humanos , Ligandos , Biblioteca de Péptidos , Péptidos/síntesis química , Péptidos/genética , Unión Proteica , Pliegue de Proteína , Rayos UltravioletaRESUMEN
Major histocompatibility complex (MHC) class I multimer technology has become an indispensable immunological assay system to dissect antigen-specific cytotoxic CD8(+) T cell responses by flow cytometry. However, the development of high-throughput assay systems, in which T cell responses against a multitude of epitopes are analyzed, has been precluded by the fact that for each T cell epitope, a separate in vitro MHC refolding reaction is required. We have recently demonstrated that conditional ligands that disintegrate upon exposure to long-wavelength UV light can be designed for the human MHC molecule HLA-A2. To determine whether this peptide-exchange technology can be developed into a generally applicable approach for high throughput MHC based applications we set out to design conditional ligands for the human MHC gene products HLA-A1, -A3, -A11, and -B7. Here, we describe the development and characterization of conditional ligands for this set of human MHC molecules and apply the peptide-exchange technology to identify melanoma-associated peptides that bind to HLA-A3 with high affinity. The conditional ligand technology developed here will allow high-throughput MHC-based analysis of cytotoxic T cell immunity in the vast majority of Western European individuals.
Asunto(s)
Antígenos HLA-A/inmunología , Antígenos de Histocompatibilidad Clase I/inmunología , Péptidos/inmunología , Ingeniería de Proteínas/métodos , Alelos , Linfocitos T CD8-positivos/inmunología , Células Clonales , Epítopos/inmunología , Antígeno HLA-A1/inmunología , Antígeno HLA-A11 , Antígeno HLA-A3/inmunología , Antígeno HLA-B7/inmunología , Humanos , Concentración 50 Inhibidora , Cinética , Ligandos , Melanoma/inmunología , Pliegue de Proteína , Estructura Cuaternaria de Proteína , Rayos UltravioletaRESUMEN
The detection of antigen-specific T cell responses by MHC multimer staining is rapidly becoming one of the core immunological techniques, and the technology to produce MHC multimers has been optimized substantially in recent years. Furthermore, recent work demonstrates the potential of high-throughput detection of T cell responses and suggests that manipulation of T cell responses through the use of multimeric MHC reagents is also feasible.
Asunto(s)
Antígenos de Histocompatibilidad Clase II/inmunología , Antígenos de Histocompatibilidad Clase I/inmunología , Linfocitos T/inmunología , Animales , Antígenos de Histocompatibilidad Clase I/química , Antígenos de Histocompatibilidad Clase II/química , Humanos , Péptidos/química , Péptidos/inmunología , Análisis por Matrices de ProteínasRESUMEN
Activated CD8+ T cells detect virally infected cells and tumor cells by recognition of major histocompatibility complex class I-bound peptides derived from degraded, endogenously produced proteins. In contrast, CD8+ T cell activation often occurs through interaction with specialized antigen-presenting cells displaying peptides acquired from an exogenous cellular source, a process termed cross-priming. Here, we observed a marked inefficiency in exogenous presentation of epitopes derived from signal sequences in mouse models. These data indicate that certain virus- and tumor-associated antigens may not be detected by CD8+ T cells because of impaired cross-priming. Such differences in the ability to cross-present antigens should form important considerations in vaccine design.
Asunto(s)
Presentación de Antígeno , Antígenos Virales/inmunología , Linfocitos T CD8-positivos/inmunología , Reactividad Cruzada , Epítopos de Linfocito T/inmunología , Animales , Antígenos Virales/genética , Línea Celular Tumoral , Células Dendríticas/inmunología , Tolerancia Inmunológica , Inmunoglobulinas/inmunología , Inmunoglobulinas/metabolismo , Virus de la Influenza A/inmunología , Activación de Linfocitos , Ratones , Papillomaviridae/inmunología , Señales de Clasificación de Proteína/genética , Proteínas Recombinantes de Fusión/inmunología , Linfocitos T Citotóxicos/inmunología , Transfección , Vacunas/inmunologíaRESUMEN
Here, we describe a new approach designed to monitor the proteolytic activity of maturing phagosomes in live antigen-presenting cells. We find that an ingested particle sequentially encounters distinct protease activities during phagosomal maturation. Incorporation of active proteases into the phagosome of the macrophage cell line J774 indicates that phagosome maturation involves progressive fusion with early and late endocytic compartments. In contrast, phagosome biogenesis in bone marrow-derived dendritic cells (DCs) and macrophages preferentially involves endocytic compartments enriched in cathepsin S. Kinetics of phagosomal maturation is faster in macrophages than in DCs. Furthermore, the delivery of active proteases to the phagosome is significantly reduced after the activation of DCs with lipopolysaccharide. This observation is in agreement with the notion that DCs prevent the premature destruction of antigenic determinants to optimize T cell activation. Phagosomal maturation is therefore a tightly regulated process that varies according to the type and differentiation stage of the phagocyte.
Asunto(s)
Presentación de Antígeno/inmunología , Catepsina B/inmunología , Catepsinas/inmunología , Células Dendríticas/enzimología , Leucina/análogos & derivados , Fagosomas/enzimología , Animales , Sitios de Unión , Catepsina B/genética , Catepsina K , Catepsina L , Catepsinas/antagonistas & inhibidores , Catepsinas/genética , Línea Celular , Células Cultivadas , Cisteína Endopeptidasas , Inhibidores de Cisteína Proteinasa/metabolismo , Inhibidores de Cisteína Proteinasa/farmacología , Células Dendríticas/inmunología , Regulación hacia Abajo , Leucina/metabolismo , Leucina/farmacología , Lipopolisacáridos/inmunología , Lipopolisacáridos/farmacología , Macrófagos/citología , Ratones , Ratones Endogámicos C57BL , Ratones Noqueados , Fagocitosis/inmunologíaRESUMEN
Antigen presentation by MHC class II molecules relies on the action of endocytic proteases, which are differentially expressed in antigen-presenting cells and are regulated by different components of the immune system. Endocytic enzymes process and convert exogenous antigens into peptidic determinants capable of interaction with MHC class II molecules. Chemical and genetic tools have recently been developed to study the role of lysosomal proteases in antigen presentation.