Simple and reliable determination of Zn and some additional elements in seminal plasma samples by using total reflection X-ray fluorescence spectroscopy.

Trace elements are essential for the normal spermatogenesis of mammals and play a critical role in sperm quality and pathological processes e.g. inflammation. Consequently, multi-elemental analysis of seminal plasma (SP) may provide significant information on physiological and pathophysiological processes occurring in the male reproductive tract. Therefore, the development of a simple, fast and reliable method for seminal plasma (SP) analysis by total reflection X-ray fluorescence spectrometry (TXRF) could be useful for both, scientific and clinical studies. In this study, a detailed assessment of the sample preparation parameters and measurement conditions, including analysis of the shape and element distribution of the deposited residue on the reflector by micro X-ray fluorescence spectrometry, was carried out. Using the best analytical conditions, limits of detection for trace elements were found to be in the range of 0.04-0.3 mg kg-1. Trueness and precision of the results, evaluated by spiked SP sample analysis, were in most cases acceptable with recovery values in the range of 87-109% and relative standard deviations 3-12% (n = 5). The developed TXRF method was applied for the analysis of several SP samples from patients with different diagnoses and the results were compared with those obtained by ICP-OES. Among the studied trace elements with a role in the antioxidant defence system only Zn could be quantified and some differences in Zn concentrations among studied groups were observed. However, further studies on a large number of samples are required to define the exact relationship between the element composition and semen quality.

Awakening cortisol indicators, advanced glycation end products, stress perception, depression and anxiety in parents of children with chronic conditions.

This cross-sectional study aims to investigate awakening cortisol indicators and to explore their association with stress perception, advanced glycation end products (AGEs), depression and anxiety in parents of children with chronic conditions. We included five parental groups according to children's diagnoses: Down syndrome (DS; N = 31), autistic spectrum disorder (ASD; N = 29), cerebral palsy (CP; N = 15), diabetes mellitus type 1 (DMT1; N = 38), and a control group of heathy children (CG; N = 33). Salivary cortisol and AGEs were measured, alongside with psychological indices. Partial correlation and multiple linear regression were used in the analysis to identify parental characteristics associated with total awakening cortisol output (area under the curve with respect to the ground, AUCG), cortisol awakening response (area under the curve with respect to the increase, AUCI), perceived stress, and health outcomes (AGEs, depression and anxiety). There was no difference between groups for AUCG, while DS and DMT1 groups showed reduced AUCI (ß = -0.241, p = 0.040; ß = -0.249, p = 0.028, respectively), and ASD parents had a borderline insignificant result (ß = -0.205, p = 0.081). Non-smokers had higher AUCG, and parental sedentary activity and sleep duration were associated with AUCI. AUCG was positively associated with AGEs (ß = 0.218, p = 0.013), anxiety (ß = 0.207, p = 0.004), and with depression (ß = 0.156, p = 0.034), unlike the AUCI. DS parental group showed lower general stress perception (ß = -0.260, p = 0.005). Parents of children with DMT1 had more pronounced depressive symptoms (ß = 0.183, p = 0.039), while CP parents had a borderline insignificant result for depression (ß = 0.143, p = 0.058). Based on these results, parents of children with chronic conditions have altered awaking cortisol response and are under increased risk of adverse health consequences.

Clinical and Biochemical Influence of Prostatic Stones.

INTRODUCTION: The study aimed to explore clinical influence of prostatic stones on lower urinary tract symptoms (LUTS), seminal plasma cytokines, and serum biomarkers. MATERIALS AND METHODS: A total of 70 men aged ≤50 years with LUTS divided into 2 groups: group with stones (GSt) and group without prostatic stones (GNoSt). All subjects completed the International Prostate Symptom Score (IPSS) questionnaire and National Institutes of Health Chronic Prostatitis Symptom Index (NIH-CPSI) scoring questionnaire. Pre- and post-prostate massage test and uroflowmetry were performed. The serum concentration of total prostate specific antigen (PSA), free PSA, and free/total PSA (f/t PSA) ratio, seminal concentration of cytokines interleukin (IL)-1ß, IL-6, IL-8, IL-10, IL-12p70, and tumor necrosis factor-alpha were measured. RESULTS: GSt subjects had significantly more severe symptoms based on IPSS answers (p = 0.0289). All domains in NIH-CPSI scores were significantly higher in the GSt group: pain (p = 0.001), urinary symptoms (p = 0.023), quality of life (p = 0.008), and with overall (p = 0.003). GSt subjects also had significantly lower maximum urinary flow (Qmax; p = 0.011), lower f/t PSA ratio (p = 0.048), and higher concentration of IL-1ß (p = 0.011) and IL-8 (p = 0.001). CONCLUSIONS: Prostatic stones may influence the severity of LUTS and the symptoms of chronic prostatitis. They might reduce Qmax rate and lead to reduction of the f/t PSA ratio and produce more severe inflammation causing increased seminal concentration of IL-1ß and IL-8.