[Morphological characteristics of the cerebellar cortex at a young age and changes in its cytoarchitectonics in opioid dependence]. / Morfologicheskaya kharakteristika kory mozzhechka v molodom vozraste i izmeneniya ee tsitoarkhitektoniki pri opioidnoi zavisimosti.

The results of histological, micrometric and immunohistochemical studies performed on sectional material of 69 men corpses aged from 21 to 29 years are presented. Two groups were identified: 42 deaths without drug addiction and 27 deaths from exposure to a toxic synthetic opioids drug, with the history their systematic use lasting from 16 months to 3 years. A comparative analysis of the morphological characteristics of cerebellar cortex tissues was carried out using staining with hematoxylin and eosin and according to the Nissl method (according to Snesarev). For immunohistochemical analysis of the samples, a panel of antibodies to the Vimentin protein was used. In each case, the distance between Purkinje cells was determined and the percentage of immunonegative Purkinje cells to Vimentin from their total number was calculated. In persons with a history of opioid dependence, signs of neurodegenerative changes in the cerebellar cortex were noted: deformation of the shape of Purkinje cells, morphological transformation of nuclei from karyopyknosis to karyorrhexis, and the appearance of fuzzy cell boundaries. There was no statistically significant difference in the distance between the Purkinje cells and their number in the opioid-dependent group and in the conditionally healthy group. An increase in the number of Purkinje cells immunopositive to the Vimentin protein was found in the group of deaths with opioid dependence. The results of assessing the cytoarchitectonics of the cerebellar cortex using an immunohistochemical method for studying Purkinje cells positively stained with antibodies to Vimentin can be used as additional criteria for forensic medical determination of the opioid dependence presence in the deceased.

[Comparative morphological characteristics of the corpus callosum in men and women of young and old age.]

The work is based on the results of an organometric study of the corpus callosum (callosometry) among 93 people (49 men and 44 women) using the method of magnetic resonance imaging. A comparative analysing of the length, height, thickness of the roller and the knee of the corpus callosum, the depth of its occurrence (front, top, back) was carried out. The regularities of age variability of organometric characteristics of the corpus callosum, manifested in a decrease in its linear dimensions among old people in comparison with young people and a decrease in the depth of its occurrence. The results of this morphological study can be as a basis for identifying individual patterns of age-related anatomy of the brain and have practical importance as indicators of the norm, which will use these data in diagnostic and therapeutic work.

[The dynamics of the structural organization of the corpus callosum from young to old age.]

The work is based on the results of histological and immunohistochemical studies performed on the sectional material of 104 human corpses (59 men and 45 women) of young and old age. A comparative analysis of the morphological characteristics of the corpus callosum tissues using hematoxylin and eosin staining, the Nissl method (by Snesarev), by Van Gieson, by Spielmeyer, by Foot. An immunohistochemical study of the samples used a panel of antibodies to glial fibrillar acid protein (GFAP), the S-100 protein. It was found that by senile age, the tissue of the corpus callosum is characterized by the accumulation of glial macrophages. With age, there is a proliferation of GFAP-immunopositive astroglia. There is no dynamics of S-100 protein expression with age. Thus, the revealed regularity of age-related variability of the cytoarchitectonics of the corpus callosum is of interest in diagnostic and therapeutic work, and its morphological picture in old age can serve as an equivalent of the anatomical norm.