RESUMEN
The constitutive androstane receptor (CAR) and the pregnane × receptor (PXR) are activated by a variety of endogenous and exogenous ligands, such as steroid hormones, bile acids, pharmaceuticals, and environmental, dietary, and occupational chemicals. In turn, they induce phase I-III detoxification enzymes and transporters that help eliminate these chemicals. Because many of the chemicals that activate CAR and PXR are environmentally-relevant (dietary and anthropogenic), studies need to address whether these chemicals or mixtures of these chemicals may increase the susceptibility to adverse drug interactions. In addition, CAR and PXR are involved in hepatic proliferation, intermediary metabolism, and protection from cholestasis. Therefore, activation of CAR and PXR may have a wide variety of implications for personalized medicine through physiological effects on metabolism and cell proliferation; some beneficial and others adverse. Identifying the chemicals that activate these promiscuous nuclear receptors and understanding how these chemicals may act in concert will help us predict adverse drug reactions (ADRs), predict cholestasis and steatosis, and regulate intermediary metabolism. This review summarizes the available data on CAR and PXR, including the environmental chemicals that activate these receptors, the genes they control, and the physiological processes that are perturbed or depend on CAR and PXR action. This knowledge contributes to a foundation that will be necessary to discern interindividual differences in the downstream biological pathways regulated by these key nuclear receptors.
RESUMEN
The constitutive androstane receptor (CAR) is a xenosensing nuclear receptor and regulator of cytochrome P450s (CYPs). However, the role of CAR as a basal regulator of CYP expression nor its role in sexually dimorphic responses have been thoroughly studied. We investigated basal regulation and sexually dimorphic regulation and induction by the potent CAR activator TCPOBOP and the moderate CAR activator Nonylphenol (NP). NP is an environmental estrogen and one of the most commonly found environmental toxicants in Europe and the United States. Previous studies have demonstrated that NP induces several CYPs in a sexually dimorphic manner, however the role of CAR in regulating NP-mediated sexually dimorphic P450 expression and induction has not been elucidated. Therefore, wild-type and CAR-null male and female mice were treated with honey as a carrier, NP, or TCPOBOP and CYP expression monitored by QPCR and Western blotting. CAR basally regulates the expression of Cyp2c29, Cyp2b13, and potentially Cyp2b10 as demonstrated by QPCR. Furthermore, we observed a shift in the testosterone 6alpha/15alpha-hydroxylase ratio in untreated CAR-null female mice to the male pattern, which indicates an alteration in androgen status and suggests a role for androgens as CAR inverse agonists. Xenobiotic-treatments with NP and TCPOBOP induced Cyp2b10, Cyp2c29, and Cyp3a11 in a CAR-mediated fashion; however NP only induced these CYPs in females and TCPOBOP induced these CYPs in both males and females. Interestingly, Cyp2a4, was only induced in wild-type male mice by TCPOBOP suggesting Cyp2a4 induction is not sensitive to CAR-mediated induction in females. Overall, TCPOBOP and NP show similar CYP induction profiles in females, but widely different profiles in males potentially related to lower sensitivity of males to either indirect or moderate CAR activators such as NP. In summary, CAR regulates the basal and chemically inducible expression of several sexually dimorphic xenobiotic metabolizing P450s in a manner that varies depending on the ligand.
Asunto(s)
Sistema Enzimático del Citocromo P-450/biosíntesis , Sistema Enzimático del Citocromo P-450/metabolismo , Inducción Enzimática/fisiología , Receptores Citoplasmáticos y Nucleares/fisiología , Factores de Transcripción/fisiología , Animales , Western Blotting , Receptor de Androstano Constitutivo , Citosol/efectos de los fármacos , Citosol/enzimología , Citosol/metabolismo , Femenino , Inmunoprecipitación , Hígado/metabolismo , Masculino , Ratones , Ratones Noqueados , Relajantes Musculares Centrales/farmacología , Parálisis/inducido químicamente , Parálisis/fisiopatología , Fenoles/farmacología , ARN/biosíntesis , ARN/genética , Receptores Citoplasmáticos y Nucleares/agonistas , Receptores Citoplasmáticos y Nucleares/genética , Reacción en Cadena de la Polimerasa de Transcriptasa Inversa , Caracteres Sexuales , Esteroide Hidroxilasas/metabolismo , Factores de Transcripción/agonistas , Factores de Transcripción/genética , Zoxazolamina/farmacologíaRESUMEN
Daphnia magna were exposed for 21 d to the ecdysteroids, 20-hydroxyecdysone (20-E), the accepted molting hormone, and ponasterone A (PoA), an ecdysteroid found in some crustaceans and many plants. Daphnids were monitored for alterations in molting, fecundity, and survival time. The 20-E elicited no significant effects on molting frequency, and its significant effects on reproduction were only at concentrations (260 nM) associated with premature death caused by incomplete ecdysis. We also examined PoA, which has been reported to have 10x higher affinity for the ecdysone receptor than 20-E. Ponasterone A elicited effects similar to those of 20-E at approximately 10x lower concentrations. This suggests that affinity for the receptor is the major parameter determining activity in vitro and in vivo and that differences in metabolism and elimination in vivo were not significant. The effects of PoA on daphnids mimicked those of 20-E except PoA reduced fecundity in the second generation and 20-E had no effect. Last, both exogenous 20-E and PoA show similar effects, including premature death associated with incomplete ecdysis, and the overall difference in toxicity is mostly likely due to receptor affinity.
Asunto(s)
Daphnia/fisiología , Ecdisterona/análogos & derivados , Ecdisterona/farmacología , Muda/efectos de los fármacos , Animales , Exposición a Riesgos Ambientales , Fertilidad/efectos de los fármacosRESUMEN
Melatonin, an indolic pineal hormone, is produced primarily at night in mammals and is important in controlling biological rhythms. Previous research suggested that melatonin can attenuate proliferation in the estrogen-responsive MCF-7 breast cancer cell line. We tested whether these anti-proliferative effects may have physiological consequences upon two estrogen-responsive cell lines, MCF-7 (a breast cancer cell line) and BG-1 (an ovarian adenocarcinoma cell line). Melatonin (10(-9)-10(-5) M) attenuated proliferation of MCF-7 and BG-1 cells by >20% in the absence of estrogen. However, 17beta-estradiol exposure negated the ability of melatonin to inhibit proliferation. To substantiate this finding, cells were estrogen starved followed by multiple treatments with estradiol and melatonin. Melatonin did not inhibit estradiol-stimulated proliferation under this protocol. Estradiol increased MCF-7 and BG-1 cell cycle transition from G1 to S phase, however, melatonin did not inhibit this transition nor did it down-regulate estradiol-induced pS2 mRNA levels measured by northern blotting, further indicating that melatonin was unable to attenuate estradiol-induced proliferation and gene expression. We also examined the effects of melatonin on estradiol-induced proliferation in MCF-7 cell xenografts in athymic nude mice. Melatonin at a dose 28 times greater than 17beta-estradiol did not inhibit estradiol-induced proliferation in vivo. Furthermore, pinealectomy did not increase proliferation. Therefore, we conclude that melatonin does not directly inhibit estradiol-induced proliferation.
Asunto(s)
División Celular/efectos de los fármacos , Estradiol/farmacología , Melatonina/farmacología , Animales , Neoplasias de la Mama/patología , Ciclo Celular/efectos de los fármacos , Femenino , Humanos , Ratones , Ratones Desnudos , Neoplasias Ováricas/patología , Proteínas/genética , ARN Mensajero/análisis , Factor Trefoil-1 , Células Tumorales Cultivadas , Proteínas Supresoras de TumorRESUMEN
Examination of estrogen-responsive processes in cell culture is used to investigate hormonal influence on cancer cell growth and gene expression. Most experimental studies have used breast cancer cell lines, in particular MCF7 cells, to investigate estrogen responsiveness. In this study we examined an ovarian cancer cell line, BG-1, which is highly estrogen-responsive in vitro. This observation, plus the fact that the cells are of ovarian rather than mammary gland origin, makes it an attractive alternative model. 17Beta-estradiol, epidermal growth factor, and insulin-like growth factorinduced proliferation of BG-1 and MCF7 cells. Viability was dependent on these growth factors in BG-1 cells, but not in MCF7 cells. Therefore, we examined the differences between these two cell lines with respect to estrogen and growth factor receptors. BG-1 cells have twice as many estrogen receptors as MCF7 cells, and BG-1 cells have higher insulin-like growth factor-1 and epidermal growth factor receptor levels than MCF7 cells. This may also explain why BG-1 cells proliferate 56% more robustly in serum and show more serum dependence in culture. In both BG-1 and MCF7 cells, epidermal growth factor receptor number is low (<20000/cell), while insulin-like growth factor-1 receptor level was highest in estrogen receptor positive cell lines. For example, insulin-like growth factor-1 receptor was higher in BG-1 and MCF7 cells than in estrogen receptor negative cells (HeLa > MDA-MB-435 > HBL100). In conclusion, BG-1 cells are an excellent model for understanding hormone responsiveness in ovarian tissue and an alternative for examining estrogen receptor-mediated and insulin-like growth factor-1/epidermal growth factor/estrogen cross-talk processes because of their sensitivity to these factors.
Asunto(s)
División Celular/fisiología , Estradiol/fisiología , Neoplasias Ováricas/patología , Células Tumorales Cultivadas , Receptores ErbB/metabolismo , Femenino , Humanos , Modelos Biológicos , Receptor IGF Tipo 1/metabolismoRESUMEN
Melatonin is proposed to be oncostatic in mammary tissue, and one mechanism by which this hormone may elicit its possible oncostatic effect is as an oxygen radical scavenger. Therefore, we examined melatonin's abilities to act as an oxygen radical scavenger at physiological or pharmacological concentrations. Hydrogen peroxide at 400 microM killed 97% of treated MCF7 cells within 8 h, and following melatonin at 10(-5) and 10(-4) M concentrations only 76 and 64% of cells, respectively, were killed by hydrogen peroxide. However, melatonin at lower concentrations (10(-7) M) did not protect MCF7 cells. Moreover, pretreatment with melatonin (10(-5) or 10(-7) M) prior to hydrogen peroxide stress offered no further efficacy, and pretreatment with melatonin followed by the withdrawal of melatonin eliminated its protective effect from hydrogen peroxide toxicity. These findings indicate that melatonin acts directly as an antioxidant and does not stimulate antioxidant defenses in MCF7 cells that protect against hydrogen peroxide. Glutathione levels were examined to substantiate this hypothesis and were not altered by melatonin treatment. In conclusion, melatonin is an excellent oxygen radical scavenger at pharmacological concentrations, but not at physiological concentrations. Thus, loss of melatonin is unlikely to be important in oxidative scavenger mechanisms in human mammary cells.
Asunto(s)
Antioxidantes/farmacología , Neoplasias de la Mama/patología , Depuradores de Radicales Libres/farmacología , Peróxido de Hidrógeno/toxicidad , Melatonina/farmacología , Oxidantes/toxicidad , Supervivencia Celular/efectos de los fármacos , Interacciones Farmacológicas , Femenino , Humanos , Células Tumorales CultivadasRESUMEN
Epidemiological studies have suggested a possible link between extremely low frequency electromagnetic fields (EMFs) and increased rates of certain cancers. One cancer that has been postulated to be associated with EMF exposure is breast cancer, for which increased rates have been reported among electricians. These cancer associations are weak, and the link to EMF exposures remains tenuous. Understanding the mechanisms by which EMFs could have biological effects will help in elucidating the risk, if any, from EMFs. One hypothesis that has received considerable attention involves reduction of melatonin levels by EMFs. This hypothesis suggests that loss of melatonin affects a variety of hormonal processes such as estrogen homeostasis and thereby may increase breast cancer rates. Since this theory was first presented, putative melatonin receptors have been cloned, providing new tools with which to examine melatonin's mechanism of action and the melatonin hypothesis. These receptors are found in nuclear and membrane fractions of cells. The nuclear receptors (retinoid Z receptors) are found both in the brain and in non-neural tissues, whereas the membrane-bound receptors are found primarily in neural tissue and have a higher affinity for melatonin. These receptors may control a variety of hormonal and immunological functions, including the release of gonadotropins from the hypothalamus and pituitary and 5-lipoxygenase activity in B lymphocytes. This Working Hypothesis briefly reviews our current knowledge of melatonin receptors and then provides theories on how the inactivation of melatonin receptors may cause cancer and suggests areas of research for addressing this question.
Asunto(s)
Neoplasias de la Mama/etiología , Neoplasias de la Mama/ultraestructura , Campos Electromagnéticos/efectos adversos , Melatonina/fisiología , Neoplasias Hormono-Dependientes/etiología , Neoplasias Hormono-Dependientes/ultraestructura , Receptores de Superficie Celular/fisiología , Receptores Citoplasmáticos y Nucleares/fisiología , Humanos , Receptores de MelatoninaRESUMEN
The ability of nonylphenol polyethoxylate (nonylphenyl polyethylene glycol, NPPG) to alter the metabolic elimination of testosterone and elicit reproductive toxicity to Daphnia magna was assessed. NPPG (5.0 mg/liter) inhibited the elimination of testosterone as glucose and sulfate conjugates, but had minimal effect on the rate of elimination of oxido-reduced and hydroxylated derivatives of the steroid hormone. This exposure concentration of NPPG also approximated the acute threshold-effect concentration and the chronic value for daphnids. Results demonstrated that NPPG qualitatively elicits similar effects on the metabolic elimination of testosterone by daphnids as previously characterized with its degradation product 4-nonylphenol. Unlike 4-nonylphenol, significant chronic toxicity of NPPG, due to effects on steroid elimination processes, was not evident. Results from the present study provide no indication that concentrations of nonylphenol polyethoxylates typically measured in the environment pose a risk of chronic toxicity to invertebrates.
Asunto(s)
Daphnia/efectos de los fármacos , Detergentes/toxicidad , Glicoles de Etileno/toxicidad , Animales , Medios de Cultivo , Daphnia/fisiología , Femenino , Glucosa/metabolismo , Fenoles/metabolismo , Sulfatos/metabolismo , Testosterona/metabolismoRESUMEN
The cytosolic glutathione S-transferases (GSTs) are dimeric enzymes that are responsible for the conjugation of glutathione to an electrophilic center of a variety of lipophilic compounds. The purpose of the present study was to characterize the GSTs of Daphnia magna with respect to enzyme multiplicity, molecular weight, isoelectric points, and immunochemical distinction and to determine the inducibility of these enzymes by the prototypical mammalian GST inducer, phenobarbital. GSTs were purified from crude cystosols prepared from daphnids by glutathione-sepharose affinity chromatography. SDS-polyacrylamide gel electrophoresis of the affinity purified GSTs revealed the presence of multiple subunits with molecular weights ranging from 26.9 to 30.2 kDa. Preparative electrofocusing separated GST activity into three major fractions having approximate isoelectric points of 4.5, 4.8 and 5.6. All of the catalytically active fractions contained a single protein band of the same molecular weight (30.2 kDa) during SDS-PAGE. A monoclonal antibody, prepared against the affinity-purified GST proteins, recognized three distinct proteins separated during analytical-scale isoelectric focusing (pI 4.6, 4.7 and 4.8). These proteins may represent a class of GSTs distinct from the GST having a pI of 5.6. Treatment of daphnids with phenobarbital elevated both GST catalytic activity and immunodetectable protein. These results demonstrate that multiple immunochemically related proteins of the same molecular weight but varying isoelectric points are responsible for most of the GST catalytic activity with the substrate 1-chloro-2,4-dinitrobenzene.
Asunto(s)
Daphnia/enzimología , Glutatión Transferasa/biosíntesis , Animales , Cromatografía de Afinidad , Citosol/enzimología , Inducción Enzimática/efectos de los fármacos , Glutatión Transferasa/inmunología , Glutatión Transferasa/aislamiento & purificación , Immunoblotting , Focalización Isoeléctrica , Isoenzimas/biosíntesis , Isoenzimas/inmunología , Isoenzimas/aislamiento & purificación , Cinética , Fenobarbital/farmacologíaRESUMEN
The aim of this study was to elucidate the effects of the compound indole-3-carbinol (I3C), which is found in cruciferous vegetables, on hepatic cholesterol homoeostasis and metabolism in male CD-1 mice. Oral administration of 500 and 750 mg I3C/kg/day to mice for 1 wk resulted in increased liver mass and microsomal protein content. Hepatic microsomal cholesterol levels were not significantly altered following treatment with 100 and 250 mg I3C/kg/day, but were significantly decreased following treatment with 500 and 750 mg/kg/day. Conversely, the lower doses of I3C administered decreased serum cholesterol levels whereas the higher doses of I3C had no effect on this parameter. Alterations in cholesterol homoeostasis by I3C were not related to liver hypertrophy, since administration of phenobarbital to mice increased liver size, but had no significant effect on hepatic microsomal or serum cholesterol levels. Activities of the hepatic enzymes cholesterol ester hydrolase and cholesterol 7 alpha-hydroxylase were not altered by I3C. However, 500 and 750 mg I3C/kg/day elevated the activity of hepatic acyl-CoA:cholesterol acyltransferase (ACAT), the enzyme responsible for the formation of hepatic cholesteryl esters. These results demonstrate that (a) I3C lowers serum cholesterol levels at concentrations that have no discernible effect on hepatic cholesterol homoeostasis, and (b) at higher doses of I3C, hepatic microsomal cholesterol levels are significantly lowered and ACAT activity is significantly elevated. These latter effects are not accompanied by changes in serum cholesterol levels and may represent compensatory mechanisms to restore cholesterol homoeostasis in the body. Mechanisms responsible for the effects of I3C on cholesterol homoeostasis are proposed.
Asunto(s)
Colesterol/química , Indoles/farmacología , Hígado/efectos de los fármacos , Animales , Colesterol/sangre , Colesterol 7-alfa-Hidroxilasa/metabolismo , Homeostasis/efectos de los fármacos , Hígado/química , Masculino , Ratones , Microsomas Hepáticos/química , Microsomas Hepáticos/efectos de los fármacos , Esterol Esterasa/metabolismo , Esterol O-Aciltransferasa/metabolismoRESUMEN
The identification and characterization of steroid-hormone-binding glutathione S-transferases (GST) were undertaken using photoaffinity-labelling techniques. Irradiation of mouse liver cytosol, in the presence of 50 nM-[3H]methyltrienolone, resulted in the specific affinity labelling of five proteins. One of these proteins, designated MBP27, had an approximate molecular mass of 27 kDa under denaturing conditions and was induced by treatment of mice with either 2(3)-t-butyl-4-hydroxyanisole (BHA) or phenobarbital (PB). An additional affinity-labelled protein, MBP25, which was not detected in untreated mouse cytosol, was induced in the liver cytosols from BHA- and PB-treated mice. The molecular masses of these proteins and their induction by BHA and PB suggested that they may be steroid-hormone-binding GST subunits. Irradiation of mouse liver cytosol in the presence of [3H]methyltrienolone, followed by immunoprecipitation using GST-specific antibodies established that both GST mu and GST alpha bind [3H]methyltrienolone and both contribute to the affinity-labelled protein designated MBP27. GST Ya1 Ya1, an alpha class GST that is not expressed in untreated mouse liver but is induced by BHA and PB, was also found to bind [3H]methyltrienolone and is identical with the affinity-labelled protein designated MBP25. Experiments were undertaken next to assess the effects of the anticarcinogenic plant compound indole-3-carbinol (I3C) on GST-mediated steroid hormone-binding using the photoaffinity labelling techniques. Treatment of mice with I3C resulted in the induction of immunoreactive GST mu and GST Ya1 Ya1. However, the steroid-binding activity of these proteins in vitro was severely inhibited by the acid-condensation products of I3C that are generated in the stomach after ingestion. These results suggest that I3C may inhibit GST-mediated steroid-binding activity which could contribute to the anticarcinogenic activity of this compound.
Asunto(s)
Glutatión Transferasa/química , Hígado/enzimología , Metribolona/química , Esteroides/metabolismo , Marcadores de Afinidad , Animales , Hidroxianisol Butilado/farmacología , Citosol/enzimología , Inducción Enzimática , Indoles/química , Masculino , Ratones , Fenobarbital/farmacología , Fotoquímica , Pruebas de Precipitina , Unión ProteicaRESUMEN
Indole-3-carbinol (I3C), a component of cruciferous vegetables, exhibits anti-carcinogenic activity in a variety of model systems. This activity has been attributed in part to the induction of cytochrome P450 CYP1A subfamily members and the resulting increased metabolic inactivation of chemical carcinogens. The present study was undertaken to assess the effects of I3C on several constitutive P450 activities that contribute to both carcinogen and steroid hormone metabolism. Mice were administered I3C in their diet at estimated daily doses of 250, 500 and 750 mg/kg for 1 week. Liver microsomes from treated and untreated mice were subsequently assayed for CYP1A-mediated ethoxy-resorufin O-deethylase (EROD) activity, estradiol 2-hydroxylase activity and seven different testosterone hydroxylase activities. I3C elevated EROD, estradiol 2-hydroxylase and testosterone 6 alpha-hydroxylase activities in a dose-dependent manner. The other six testosterone hydroxylase activities were not significantly affected by in vivo treatment with I3C. In addition to its effects on steroid hydroxylase activities, I3C also elevated NADPH-cytochrome P450 reductase activity, a necessary component to the P450 monooxygenase system. We next examined the direct in vitro effects of I3C and its acid condensation products, as are generated in the stomach following ingestion, on the P450 catalytic activities. Testosterone 6 beta-hydroxylase, the major testosterone hydroxylase activity in untreated mice, was significantly inhibited (IC50 approximately 12 micrograms/ml) by the acid condensation products of I3C. In contrast, all other P450 activities were not appreciably affected by I3C or its acid condensation products. These results indicate that I3C can elicit both inductive and suppressive effects on the constitutive P450s that participate in carcinogen and steroid hormone metabolism. This pleiotropic effect on hepatic catalytic enzymes may contribute to the anti-carcinogenic properties of this compound.