Serological Survey of Mosquito-Borne Arboviruses in Wild Birds from Important Migratory Hotspots in Romania.

In the context of climate change, globalization, and enhanced human traveling, arboviruses continue to represent a threat to public health. West Nile and Usutu viruses are mosquito-borne viruses belonging to the Flaviviridae family (Flavivirus genus) and members of the Japanese encephalitis virus serocomplex. Included in the Togaviridae family (Alphavirus genus), the Sindbis virus is also vectored by mosquitoes. In the present study, we aimed to analyze the presence of antibodies concerning the abovementioned viruses in migratory and resident birds in the South-Eastern region of Romania, as avian hosts represent the main reservoir for human infection. Blood samples were collected from wild birds between May 2018 and October 2019 in nine locations from three counties. All the samples were serologically tested by ELISA and a serum neutralization test. Overall, a seroprevalence of 8.72% was registered for the West Nile virus, 2.71% for the Usutu virus, and 0% for the Sindbis virus. To our best knowledge, this is the first large-scale comprehensive study to assess the West Nile virus seropositivity in wild birds and the first serological confirmation of the Usutu virus in wild birds in Romania. Moreover, this is the only follow-up study reviewing the current seroprevalence of the Sindbis virus in Romania since 1975.

Limited reciprocal surrogacy of bird and habitat diversity and inconsistencies in their representation in Romanian protected areas.

Because it is impossible to comprehensively characterize biodiversity at all levels of organization, conservation prioritization efforts need to rely on surrogates. As species distribution maps of relished groups as well as high-resolution remotely sensed data increasingly become available, both types of surrogates are commonly used. A good surrogate should represent as much of biodiversity as possible, but it often remains unclear to what extent this is the case. Here, we aimed to address this question by assessing how well bird species and habitat diversity represent one another. We conducted our study in Romania, a species-rich country with high landscape heterogeneity where bird species distribution data have only recently started to become available. First, we prioritized areas for conservation based on either 137 breeding bird species or 36 habitat classes, and then evaluated their reciprocal surrogacy performance. Second, we examined how well these features are represented in already existing protected areas. Finally, we identified target regions of high conservation value for the potential expansion of the current network of reserves (as planned under the new EU Biodiversity Strategy for 2030). We found a limited reciprocal surrogacy performance, with bird species performing slightly better as a conservation surrogate for habitat diversity than vice versa. We could also show that areas with a high conservation value based on habitat diversity were represented better in already existing protected areas than areas based on bird species, which varied considerably between species. Our results highlight that taxonomic and environmental (i.e., habitat types) data may perform rather poorly as reciprocal surrogates, and multiple sources of data are required for a full evaluation of protected areas expansion.

Zebrafish as a Screening Model to Study the Single and Joint Effects of Antibiotics.

The overuse of antibiotics combined with the limitation of wastewater facilities has resulted in drug residue accumulation in the natural environment. Thus, in recent years, the presence of antibiotic residues in the environment has raised concerns over the potential harmful effects on ecosystems and human health. The in vivo studies represent an essential step to study the potential impact induced by pharmaceutical exposure. Due to the limitations of traditional vertebrate model systems, zebrafish (Danio rerio) has recently emerged as a promising animal model to study the toxic effects of drugs and their therapeutic efficacy. The present review summarizes the recent advances made on the toxicity of seven representative classes of antibiotics, namely aminoglycosides, ß-lactams, macrolides, quinolones, sulfonamides, tetracyclines and polyether antibiotics, in zebrafish, as well as the combined effects of antibiotic mixtures, to date. Despite a significant amount of the literature describing the impact of single antibiotic exposure, little information exists on the effects of antibiotic mixtures using zebrafish as an animal model. Most of the research papers on this topic have focused on antibiotic toxicity in zebrafish across different developmental stages rather than on their efficacy assessment.

A review of constraints and solutions for collecting raptor samples and contextual data for a European Raptor Biomonitoring Facility.

The COST Action 'European Raptor Biomonitoring Facility' (ERBFacility) aims to develop pan-European raptor biomonitoring in support of better chemicals management in Europe, using raptors as sentinel species. This presents a significant challenge involving a range of constraints that must be identified and addressed. The aims of this study were to: (1) carry out a comprehensive review of the constraints that may limit the gathering in the field of raptor samples and contextual data, and assess their relative importance across Europe; and (2) identify and discuss possible solutions to the key constraints that were identified. We applied a participatory approach to identify constraints and to discuss feasible solutions. Thirty-one constraints were identified, which were divided into four categories: legal, methodological, spatial coverage, and skills constraints. To assess the importance of the constraints and their possible solutions, we collected information through scientific workshops and by distributing a questionnaire to stakeholders in all the countries involved in ERBFacility. We obtained 74 answers to the questionnaire, from 24 of the 39 COST participating countries. The most important constraints identified were related to the collection of complex contextual data about sources of contamination, and the low number of existing raptor population national/regional monitoring schemes and ecological studies that could provide raptor samples. Legal constraints, such as permits to allow the collection of invasive samples, and skills constraints, such as the lack of expertise to practice necropsies, were also highlighted. Here, we present solutions for all the constraints identified, thus suggesting the feasibility of establishing a long-term European Raptor Sampling Programme as a key element of the planned European Raptor Biomonitoring Facility.

Common Buzzards wintering strategies as an effect of weather conditions and geographic barriers.

AIM: Migration is a constantly changing adaptation due to the climate condition evolution. The struggle for surviving during harsh winter season is different across Europe, being more complex toward the inner parts of the continent. The current approach explores the Common Buzzard number variation during the cold season and the climatic predictors of birds of prey wintering movements in relation to the possible influences of the Carpathian Mountains, which may act as a geographical barrier providing shelter from cold air outbreak from north and northeast of the continent. LOCATION: Romania (45°N25°E). TAXON: Birds of Prey. METHODS: We applied a GLMM to investigate the relation between continental and local climatic factors with the number of Common Buzzard observations in two regions. The first region is located inside the Carpathian Arch and the other one outside, east of this large mountains chain. RESULTS: The Common Buzzard numbers wintering Eastern from the Carpathian Mountains are highly influenced by AO (Z = 2.87, p < .05%), while those wintering western are influenced by NAO (Z = 2.17, p < .05%). This is the first proof of separating influences for biodiversity of AO and NAO at continental scale, outlining the influence limit placed over the Eastern Carpathian Mountains. MAIN CONCLUSIONS: The Carpathian Mountains act like a geographic barrier, separating the wintering Common Buzzard populations from both sides of the mountain range. While the high number of individuals in Moldova is related to their eastern and northeastern Europe origins, in Transylvania the large number of individuals observed is related to the more sheltered characteristics of the region attracting individuals from central Europe. Also, since Transylvania region is well sheltered during cold air outbreak, it represents a more favorable region for wintering. From this point of view, we can consider that the Carpathian Mountains are a geographic barrier for wintering birds of prey.

Response of aquatic macroinvertebrates communities to multiple anthropogenic stressors in a lowland tributary river.

In the current study the response of aquatic macroinvertebrate communities to multiple anthropogenic stressors in a typical lowland river that crosses pristine sectors situated toward headwaters, as well as densely populated urban areas was assessed. We wished to develop an effective bioassay procedure for assessing water and sediment quality in lotic ecosystems from Romania with the aid of macroinvertebrate organisms correlated with physico-chemical parameters and pollutants in both dissolved fractions and material bonded to sediment. A fast scanning approach of the river, from springs to the mouth, was employed. We found significant changes in physico-chemical parameters along a longitudinal gradient, the highest values being registered within the urban area and heavily agriculturally developed areas. The macroinvertebrates showed affinities for certain abiotic factors, emphasising their potential use for future studies as reliable ecological indicators, shaped by a synergic combination of urban effects and magnitude of type of land use.

Preliminary investigation of lower Danube pollution caused by potentially toxic metals.

The current study aims to assess the pollution status of the European river-sea system lower Danube River-Danube Delta-North West Black Sea, through an integrated analysis of metal concentrations in water, sediments and fish community. The Danube flows through numerous industrial cities and receives a significant amount of pollutants due to the reception of urban and industrial emissaries, as well as agricultural land runoff. Samples of water, sediments and fish (10 species) were collected from 7 representative sites along Danube River, Danube Delta and Black Sea shore. For the analysed fish species, potentially toxic and essential elements (Pb, Cd, As, Cu, Fe, Zn, Mg, Ca, Na, K) from muscle and liver samples were measured and discussed. Measurement of elements and other environmental quality parameters were determined for water and sediments. The Black Sea area, represented by S6 and S7, received sediments from Danube with the lowest concentrations of Cd (0.05 ± 0.01 µg g-1, respectively 0.01 ± 0.001 µg g-1), Pb (3 ± 0.03 µg g-1, respectively 2 ± 0.03 µg g-1), As (2 ± 0.02 µg g-1, respectively 1.4 ± 0.3 µg g-1), Ni (8.9 ± 0.1 µg g-1, respectively 5.2 ± 0.2 µg g-1), Cr (8 ± 0.7 µg g-1, respectively 5 ± 0.2 µg g-1), Cu (3 ± 0.1 µg g-1, respectively 2 ± 0.04 µg g-1), Fe(6 ± 0.3 µg g-1, respectively 3 ± 0.1 µg g-1) and Zn (0.03 ± 0.003 µg g-1, respectively 0.017 ± 0.001 µg g-1). These results suggest that the Danube Delta system plays an important role in filtering the pollutants. Based on the biota and water analysis, there was no correlation observed between Cd, respectively Pb concentration in the environment and fish body (Person Coef. = -0.02 in muscle tissue and -0.01 in liver tissue, respectively Pearson Coeff. = -0.06 in muscle tissue and 0.1 in liver tissue). Cadmium remained an active element in the pollution of the Danube area (S1 and S2), with high concentration in the water matrix (0.14 ± 0.02 µg L-1, respectively 0.05 ± 0.01 µg L-1) and fish muscle (0.15 ± 0.03 µg g-1f.w. in C. carpio - S2). This fact was confirmed by several other studies.

Patterns of toxic metals bioaccumulation in a cross-border freshwater reservoir.

In freshwater ecosystems toxic metals can follow different routes of bioaccumulation in the organisms' body mass, routes that are similar to electronic circuits, but far more complex due to their hierarchy levels. Reservoirs located on river courses have positive impacts on economic and social development because they concentrate large water volumes used for electrical energy production, water supply, irrigation, industry, aquaculture, providing ecosystems for migratory birds and aquatic species. The aim of the study was to assess the contamination with copper (Cu), cadmium (Cd), lead (Pb), chromium (Cr) and nickel (Ni) of a cross-border reservoir located on River Prut (border between Romania and Republic of Moldova) in a temperate climate zone. Several aspects were considered: seasonal variation of water parameters and toxic metals, bioconcentration in eight fish species and transfer from prey to predator through the food chain, bioaccumulation by two freshwater molluscs separated in size groups and their role as bioindicators of toxic metals pollution. Metals concentrations were measured with an atomic absorption spectrometer (HR-CS GF-AAS). Cadmium, lead, nickel and chromium concentrations limits in water samples were below the detection limits (0.005 µg L-1Cd, 0.013 µg L-1Pb and 0.011 µg L-1Cr) during the monitoring period, suggesting that anthropogenic contamination was insignificant in the studied ecosystem. Fish liver and kidney had specific selectivity for copper (0.9-55.56 µg g-1) and cadmium (0.097-1.031 µg g-1) in case of pike-perch and bighead carp. The separation of molluscs in size groups did not prove that toxic metals increase in concentration with the organism age.