Canine oocyte nuclear maturation with Nano-ozone (NZS) supplementation: The alterations of antioxidant, and oxidant status and CDK1, cyclin B1 expressions.

This study aims to evaluate the effects of nano-ozone solution (NZS) on canine oocyte nuclear maturation, associated with the alterations of antioxidant and oxidant status and cyclin-dependent kinase 1 (CDK1), cyclin B1 gene expressions. Oocytes were cultured in four distinct concentrations of NZS (0.5, 1, 2, and 5 µg/mL) and parthenogenetically activated. The rates of oocytes arrested at the Germinal Vesicle (GV), Germinal Vesicle Breakdown (GVBD), Metaphase I (MI), and Metaphase II (MII) stages were statistically different among groups (P < 0.05). The oocytes cultured in 1 µg/mL NZS yielded the best oocyte maturation rate at the MI and MII stages; however, the lowest maturation and high degeneration rates were observed in Group E. The measurements of Malondialdehyde (MDA), reduced Glutathione (GSH), Superoxide Dismutase (SOD), and Ferric Reducing/Antioxidant Power assay (FRAP) were performed from IVM culture media. No statistical difference was observed in SOD and MDA results (P > 0.05). GSH levels were statistically significant between Group A-Group E (p = 0.003), Group B-Group E (p = 0.045), and Group E-Group D (p = 0.021). The culture media in Group D and Group E had high FRAP concentrations and significantly differed between groups (P < 0.05). CDK1, and cyclin B1 genes, which are subunits of maturation-promoting factor (MPF), are upregulated in Group B and Group C, while are downregulated in oocytes of Group E. This study showed that low, controlled doses of NZS (1 µg/mL) supplementation could improve the meiotic competence of canine oocytes and lead to positive response in expressions of CDK1 and cyclin B1 on the gene level.

Vincristine-associated total antioxidant and oxidant status of ovaries and in vitro nuclear oocyte maturation in dogs with canine transmissible venereal tumor.

The aim of this study is to evaluate the effects of scheduled vincristine sulfate therapy on canine oocyte quality and nuclear oocyte maturation, associated with total antioxidant and oxidant status of ovaries and Anti-Müllerian Hormone (AMH) concentrations in dogs with Canine Transmissible Venereal Tumor (CTVT). Six bitches suffering from CTVT and six healthy bitches were included in the study. Hemogram was carried out weekly. AMH measurements and ovariohysterectomy operations were performed after the termination of vincristine sulfate therapies. Tissue samples from ovaries were utilized for Malondialdehyde (MDA), reduced Glutathione (GSH), Superoxide Dismutase (SOD), Total Anti-oxidative Status (TAS), Total Oxidative Status (TOS) measurements, and Oxidative Stress Index (OSI) was calculated. Collected oocytes were evaluated for meiotic competence, after In Vitro Maturation (IVM) and parthenogenetic activation. No difference between the two groups was observed in hematologic parameters (P > 0.05). Meiotic stages of Germinal Vesicle Break Down (GVBD), Metaphase I (MI), and Metaphase II (MII) were significantly different between groups (P < 0.05). The number of oocytes reaching MII and meiotic resumption was lower in the CTVT group. Furthermore, AMH concentrations, oxidant parameters (OSI, TOS, and MDA), and antioxidant parameters (GSH, SOD, and TAS) were also statistically different between groups (P < 0.05). The results of this study show that vincristine sulfate application in the treatment of CTVT could alter oxidant/antioxidant status in ovaries. Apart from these, oocyte quality and IVM rates seem to decline related to gonadotoxicity. Moreover, AMH could be an important marker in the evaluation of oocyte qualities in bitches, as it is in women.

Isotretinoin therapy for the treatment of acne in patients with cystic fibrosis: a case series and review of the literature.

BACKGROUND: Cystic fibrosis (CF) is the most common severe autosomal recessive disorder in Caucasians. Viscous secretionstypically obstruct the lungs, pancreas, and gastrointestinal tract. As disease management improves, patients will increasingly seek care for conditions such as acne. Isotretinoin therapy for acne in patients with CF is controversial owing to concerns that the medication may exacerbate CF-related hepatic, pulmonary, and ocular complications. PURPOSE: We describe two patients with CF treated with isotretinoin from our clinic and also provide a literature review of 11 similar cases. We describe patient outcomes, common complications, and the risks for severe adverse effects. MATERIALS AND METHODS: The clinical courses of two patients with CF who were treated with isotretinoin for moderate-severe acne are presented. Using PubMed, we analyzed previous case reports of patients with CF who were prescribed isotretinoin and review complications associated with systemic retinoids. RESULTS: Based on a synthesis of the literature and our own experience, it appears that isotretinoin therapy for CF patients with moderate-severe acne may be an appropriate option when clinically indi ated. If dermatologists monitor lab values and adverseeffects carefully, patients with CF can benefit from isotretinoin therapy.

Management of gallstones and its related complications.

The majority of gallstone patients remain asymptomatic; however, interest toward the gallstone disease is continuing because of the high worldwide prevalence and management costs and the development of gallstone symptoms and complications. For cholesterol gallstone disease, moreover, a strong link exists between this disease and highly prevalent metabolic disorders such as obesity, dyslipidemia, type 2 diabetes, hyperinsulinemia, hypertriglyceridemia and the metabolic syndrome. Information on the natural history as well as the diagnostic, surgical (mainly laparoscopic cholecystectomy) and medical tools available to facilitate adequate management of cholelithiasis and its complications are, therefore, crucial to prevent the negative outcomes of gallstone disease. Moreover, some risk factors for gallstone disease are modifiable and some preventive strategies have become necessary to reduce the onset and the severity of complications.

Pili annulati / Pili annulati

Se presenta una paciente de 16 años de edad con pili annulati. Sus cabellos presentan zonas normales alternando con otras enfermas. Lo más característico era la presencia de bandas claras de 1 a 3 mm de longitud que también alternaba con bandas más oscuras. Estas zonas claras son debidas a la presencia de cavidades localizadas al azar en el tallo piloso. El tamaño de las cavidades varía entre 1 a 10 micrómetros. Las zonas claras se producen por reflexión de la luz en las cavidades llenas de aire. Nuestra paciente no presentaba antecedentes familiares ni alteraciones clínicas o de laboratorio. Son pocos los casos publicados en la literatura extranjera y no lo hemos encontrado publicado en la literatura argentina (AU)

Pili annulati / Pili annulati

Se presenta una paciente de 16 años de edad con pili annulati. Sus cabellos presentan zonas normales alternando con otras enfermas. Lo más característico era la presencia de bandas claras de 1 a 3 mm de longitud que también alternaba con bandas más oscuras. Estas zonas claras son debidas a la presencia de cavidades localizadas al azar en el tallo piloso. El tamaño de las cavidades varía entre 1 a 10 micrómetros. Las zonas claras se producen por reflexión de la luz en las cavidades llenas de aire. Nuestra paciente no presentaba antecedentes familiares ni alteraciones clínicas o de laboratorio. Son pocos los casos publicados en la literatura extranjera y no lo hemos encontrado publicado en la literatura argentina

[Erythema dyschromicum perstans versus lichen planus]. / Eritema discrómico persistente versus liquen plano.

Erythema dyschromicum perstans and lichen planus have been regarded as related. We describe the case of a 41 year old man presenting a relapsing dermatitis consisting of erythematous patches leaving behind ashy-gray macules. The lesions of lichen planus were atypical and the ashy dermatosis was transient. The possibility that some cases of lichen planus lead to an erythema dyschromicum perstans is discussed.

Carcinoma adenoquístico de las glándulas salivales menores / Adenocystic carcinoma of the minor salivary glands

El carcinoma adenoquístico es un tumor que afecta preferentemente las glándulas salivales, pero puede aparecer en otros tejidos incluyendo la piel. Su histología es característica. Se presenta un caso típico originado en las glándulas salivales menore del paladar duro, en una mujer de 63 años (AU)

Carcinoma adenoquístico de las glándulas salivales menores / Adenocystic carcinoma of the minor salivary glands

El carcinoma adenoquístico es un tumor que afecta preferentemente las glándulas salivales, pero puede aparecer en otros tejidos incluyendo la piel. Su histología es característica. Se presenta un caso típico originado en las glándulas salivales menore del paladar duro, en una mujer de 63 años