Dental wear and oral pathology among sex determined Early Bronze-Age children from Franzhausen I, Lower Austria.

The physical properties of diet and oral health throughout childhood play an important role in the development of human dentition, and differed greatly before the industrial revolution. In this study we examined dental wear and oral pathology in a sample of children from the Early Bronze-Age to investigate the physical and mechanical properties of childhood diet and related oral health. We explore cross-sectional age and sex-based variation of children in the sample. The analysis was carried out on the dentitions of 75 children, 978 teeth, excavated from the Early Bronze-Age cemetery Franzhausen I in Lower Austria. Presence of dental caries and calculus was recorded. Dental wear was measured using dentine exposure, occlusal topography, and dental microwear texture analysis. Sex determination was carried out using amelogenin peptide analysis. Caries were found in only 4 individuals (crude prevalence rate-5%, 95% CI 1% to 13%), affecting only 5 teeth (true prevalence rate-less than 1%). Dentine exposure was observed in over 70% of deciduous molars and dental wear measurements indicate a comparatively strong dental wear accumulation especially, among younger children, when compared to modern-day and later pre-industrial populations. Microwear textures presented a high complexity (Asfc > 2)/low anisotropy (epLsar < 1) profile, especially in older children. Differences between male and female children were not generally significant but increased dentine exposure was observed in the lower molars of younger female children. Our results suggest that the Early Bronze-Age children at Franzhausen I consumed a non-cariogenic diet, more abrasive and inclusive of harder/polyhedral foodstuffs than present-day children and some later Medieval children. Differences in dental wear accumulation were observed between children within the population, but with minimal variation between the sexes mostly occurring among younger children.

Quantification of dental macrowear using 3D occlusal surface topographic measurements in deciduous and permanent molars of children.

OBJECTIVES: Childhood paleodietary reconstruction via dental macrowear analysis is limited in part by available methods to measure dental macrowear. We describe a method to quantify dental macrowear progression (in both deciduous and permanent molars) using a handheld intraoral scanner and two 3D occlusal topographic measurements. We assess the agreement of our macrowear proxies with an established qualitative wear scoring system and their relationship to age. MATERIAL AND METHODS: We scanned 92 well-preserved dentitions of immature individuals from the medieval cemetery of St. Pölten in Lower Austria using an intraoral scanner. Two measurements were made on the resulting mesh files-the relative flat surface area in % of the occlusal surface (RFSA%) and the mesial interior slope angle. We estimated the technical error of measurement (TEM). Comparisons were made with the macrowear scoring system-tooth wear index. RESULTS: We found that TEM for both measurements was between 1 and 3%, except the interobserver TEM of RFSA% which was above 5%. Both quantitative measurements generally agree with the established qualitative scores and correlate with age; however, RFSA% does not reliably indicate the progression of macrowear for teeth after dentine exposure occurs. DISCUSSION: The proposed 3D topographic measurements can be made reliably, and within a certain range of wear provide good quantitative proxies of the progression of dental macrowear. Such measurements constitute a promising approach for improving dental macrowear analysis in contexts such as childhood paleodietary reconstruction, which benefit from additional precision in wear rate estimation and present less dentine exposure.