Neurobehavioral effects of fungicides in zebrafish: a systematic review and meta-analysis.

Pesticides are widely used in global agriculture to achieve high productivity levels. Among them, fungicides are specifically designed to inhibit fungal growth in crops and seeds. However, their application often results in environmental contamination, as these chemicals can persistently be detected in surface waters. This poses a potential threat to non-target organisms, including humans, that inhabit the affected ecosystems. In toxicologic research, the zebrafish (Danio rerio) is the most commonly used fish species to assess the potential effects of fungicide exposure, and numerous and sometimes conflicting findings have been reported. To address this, we conducted a systematic review and meta-analysis focusing on the neurobehavioral effects of fungicides in zebrafish. Our search encompassed three databases (PubMed, Scopus, and Web of Science), and the screening process followed predefined inclusion/exclusion criteria. We extracted qualitative and quantitative data, as well as assessed reporting quality, from 60 included studies. Meta-analyses were performed for the outcomes of distance traveled in larvae and adults and spontaneous movements in embryos. The results revealed a significant overall effect of fungicide exposure on distance, with a lower distance traveled in the exposed versus control group. No significant effect was observed for spontaneous movements. The overall heterogeneity was high for distance and moderate for spontaneous movements. The poor reporting practices in the field hindered a critical evaluation of the studies. Nevertheless, a sensitivity analysis did not identify any studies skewing the meta-analyses. This review underscores the necessity for better-designed and reported experiments in this field.

Systematic review and meta-analysis of 10 years of unpredictable chronic stress in zebrafish.

The zebrafish (Danio rerio) is a model animal that is being increasingly used in neuroscience research. A decade ago, the first study on unpredictable chronic stress (UCS) in zebrafish was published, inspired by protocols established for rodents in the early 1980s. Since then, several studies have been published by different groups, in some cases with conflicting results. Here we conducted a systematic review to identify studies evaluating the effects of UCS in zebrafish and meta-analytically synthetized the data of neurobehavioral outcomes and relevant biomarkers. Literature searches were performed in three databases (PubMed, Scopus and Web of Science) with a two-step screening process based on inclusion/exclusion criteria. The included studies underwent extraction of qualitative and quantitative data, as well as risk-of-bias assessment. Outcomes of included studies (n = 38) were grouped into anxiety/fear-related behavior, locomotor function, social behavior or cortisol level domains. UCS increased anxiety/fear-related behavior and cortisol levels while decreasing locomotor function, but a significant summary effect was not observed for social behavior. Despite including a substantial number of studies, the high heterogeneity and the methodological and reporting problems evidenced in the risk-of-bias analysis made it difficult to assess the internal validity of most studies and the overall validity of the model. Our review thus evidences the need to conduct well-designed experiments to better evaluate the effects of UCS on diverse behavioral patterns displayed by zebrafish.

Genotypic and Phenotypic Characterization of Antimicrobial and Heavy Metal Tolerance in Salmonella enterica and Escherichia coli Isolates from Swine Feed Mills.

Antimicrobials and heavy metals are commonly used in the animal feed industry. The role of in-feed antimicrobials on the evolution and persistence of resistance in enteric bacteria is not well described. Whole-Genome Sequencing (WGS) is widely used for genetic characterizations of bacterial isolates, including antimicrobial resistance, heavy metal tolerance, virulence factors, and relatedness to other sequenced isolates. The goals of this study were to i) use WGS to characterize Salmonella enterica (n = 33) and Escherichia coli (n = 30) isolated from swine feed and feed mill environments; and ii) investigate their genotypic and phenotypic antimicrobial and heavy metal tolerance. Salmonella isolates belonged to 10 serovars, the most common being Cubana, Senftenberg, and Tennessee. E. coli isolates were grouped into 22 O groups. Phenotypic resistance to at least one antimicrobial was observed in 19 Salmonella (57.6%) and 17 E. coli (56.7%) isolates, whereas multidrug resistance (resistant to ≥3 antimicrobial classes) was observed in four Salmonella (12%) and two E. coli (7%) isolates. Antimicrobial resistance genes were identified in 17 Salmonella (51%) and 29 E. coli (97%), with 11 and 29 isolates possessing genes conferring resistance to multiple antimicrobial classes. Phenotypically, 53% Salmonella and 58% E. coli presented resistance to copper and arsenic. All isolates that possessed the copper resistance operon were resistant to the highest concentration tested (40 mM). Heavy metal tolerance genes to copper and silver were present in 26 Salmonella isolates. Our study showed a strong agreement between predicted and measured resistances when comparing genotypic and phenotypic data for antimicrobial resistance, with an overall concordance of 99% and 98.3% for Salmonella and E. coli, respectively.

Industrial wastewater treatment by plant-based bio-filtration.

Constructed wetlands (CWs) represent a natural wastewater treatment process, offering economic and environmental advantages. These systems can remove several components that may cause negative impacts on the environment. Media types and plant species are crucial influencing factors for the removal of contaminants in CWs. The goal of this study is to evaluate the capacity of a CW using Tamarix spp. with three filter media to treat FGD wastewater. Planted and unplanted CWs were set up with varying types of biofilm support media: 3 bioreactors were operated with 50% gravel and 50% zeolite (v/v), 3 with 100% gravel, and 3 with 50% gravel, 25% zeolite, and 25% silage. Planted CWs had the greatest potential to reduce the concentrations of B, K, and NH4+-N in 64.9%, 91.1%, and 92.5%, respectively, when used in addition to the filter composed by 50% gravel + 50% zeolite, which was the only media keeping the plants alive for 60 days. The results showed that the optimal selection of filter media depends on the purpose for which the treatment has been projected for, considering that the types of substrates influenced the nature of the contaminant removal in the CW.

Salinity impact on Constructed wetlands (CWs) is still scarce in the literature. The novelty is the choice of a salt cedar (Tamarix spp.) combined with three filter media types ((1) gravel; (2) gravel and zeolite; (3) gravel, zeolite, and silage) to treat flue gas desulfurization wastewater in CWs. Our findings demonstrate that filter media containing 50% gravel + 50% zeolite can decrease the toxicity of contaminants from FGD wastewater in plants.

Ochratoxin A induces locomotor impairment and oxidative imbalance in adult zebrafish.

Ochratoxin A (OTA) is a mycotoxin produced by species of filamentous fungi widely found as a contaminant in food and with high toxic potential. Studies have shown that this toxin causes kidney and liver damage; however, data on the central nervous system effects of exposure to OTA are still scarce. Thus, this study aimed to investigate the effects of exposure to OTA on behavioral and neurochemical parameters in adult zebrafish. The animals were treated with different doses of OTA (1.38, 2.77, and 5.53 mg/kg) with intraperitoneal injections and submitted to behavioral evaluations in the open tank and social interaction tests. Subsequently, they were euthanized, and the brains were used to assess markers associated with oxidative status. In the open tank test, OTA altered distance traveled, absolute turn angle, mean speed, and freezing time. However, no significant effects were observed in the social interaction test. Moreover, OTA also increased glutathione peroxidase (GPx), glutathione-S-transferase (GST), and glutathione reductase (GR) levels and decreased non-protein thiols (NPSH) levels in the zebrafish brain. This study showed that OTA can affect behavior and neurochemical levels in zebrafish.

Early-season plant-to-plant spatial uniformity can affect soybean yields.

Increased soybean (Glycine max L. Merril) seed costs have motivated interest in reduced seeding rates to improve profitability while maintaining or increasing yield. However, little is known about the effect of early-season plant-to-plant spatial uniformity on the yield of modern soybean varieties planted at reduced seeding rates. The objectives of this study were to (i) investigate traditional and devise new metrics for characterizing early-season plant-to-plant spatial uniformity, (ii) identify the best metrics correlating plant-to-plant spatial uniformity and soybean yield, and (iii) evaluate those metrics at different seeding rate (and achieved plant density) levels and yield environments. Soybean trials planted in 2019 and 2020 compared seeding rates of 160, 215, 270, and 321 thousand seeds ha-1 planted with two different planters, Max Emerge and Exact Emerge, in rainfed and irrigated conditions in the United States (US). In addition, trials comparing seeding rates of 100, 230, 360, and 550 thousand seeds ha-1 were conducted in Argentina (Arg) in 2019 and 2020. Achieved plant density, grain yield, and early-season plant-to-plant spacing (and calculated metrics) were measured in all trials. All site-years were separated into low- (2.7 Mg ha-1), medium- (3 Mg ha-1), and high- (4.3 Mg ha-1) yielding environments, and the tested seeding rates were separated into low (< 200 seeds m-2), medium (200-300 seeds m-2), and high (> 300 seeds m-2) levels. Out of the 13 metrics of spatial uniformity, standard deviation (sd) of spacing and of achieved versus targeted evenness index (herein termed as ATEI, observed to theoretical ratio of plant spacing) showed the greatest correlation with soybean yield in US trials (R2 = 0.26 and 0.32, respectively). However, only the ATEI sd, with increases denoting less uniform spacing, exhibited a consistent relationship with yield in both US and Arg trials. The effect of spatial uniformity (ATEI sd) on soybean yield differed by yield environment. Increases in ATEI sd (values > 1) negatively impacted soybean yields in both low- and medium-yield environments, and in achieved plant densities below 200 thousand plants ha-1. High-yielding environments were unaffected by variations in spatial uniformity and plant density levels. Our study provides new insights into the effect of early-season plant-to-plant spatial uniformity on soybean yields, as influenced by yield environments and reduced plant densities.

Micronized Curcumin Causes Hyperlocomotion in Zebrafish Larvae.

Zebrafish larvae have been widely used in neuroscience and drug research and development. In the larval stage, zebrafish present a broad behavioral repertoire and physiological responses similar to adults. Curcumin (CUR), a major component of Curcuma longa L. (Zingiberaceae), has demonstrated the ability to modulate several neurobiological processes relevant to mental disorders in animal models. However, the low bioavailability of this compound can compromise its in vivo biological potential. Interestingly, it has been shown that micronization can increase the biological effects of several compounds. Thus, in this study, we compared the effects of acute exposure for 30 min to the following solutions: water (control), 0.1% DMSO (vehicle), 1 µM CUR, or 1 µM micronized curcumin (MC) in zebrafish larvae 7 days post-fertilization (dpf). We analyzed locomotor activity (open tank test), anxiety (light/dark test), and avoidance behavior (aversive stimulus test). Moreover, we evaluated parameters of oxidative status (thiobarbituric acid reactive substances and non-protein thiols levels). MC increased the total distance traveled and absolute turn angle in the open tank test. There were no significant differences in the other behavioral or neurochemical outcomes. The increase in locomotion induced by MC may be associated with a stimulant effect on the central nervous system, which was evidenced by the micronization process.

Genotypic and Phenotypic Characterization of Antimicrobial Resistance Profiles in Non-typhoidal Salmonella enterica Strains Isolated From Cambodian Informal Markets.

Non-typhoidal Salmonella enterica is a pathogen of global importance, particularly in low and middle-income countries (LMICs). The presence of antimicrobial resistant (AMR) strains in market environments poses a serious health threat to consumers. In this study we identified and characterized the genotypic and phenotypic AMR profiles of 81 environmental S. enterica strains isolated from samples from informal markets in Cambodia in 2018-2019. AMR genotypes were retrieved from the NCBI Pathogen Detection website (https://www.ncbi.nlm.nih.gov/pathogens/) and using ResFinder (https://cge.cbs.dtu.dk/services/) Salmonella pathogenicity islands (SPIs) were identified with SPIFinder (https://cge.cbs.dtu.dk/services/). Susceptibility testing was performed by broth microdilution according to the Clinical and Laboratory Standards Institute (CLSI) standard guidelines M100-S22 using the National Antimicrobial Resistance Monitoring System (NARMS) Sensititre Gram Negative plate. A total of 17 unique AMR genes were detected in 53% (43/81) of the isolates, including those encoding tetracycline, beta-lactam, sulfonamide, quinolone, aminoglycoside, phenicol, and trimethoprim resistance. A total of 10 SPIs (SPI-1, 3-5, 8, 9, 12-14, and centisome 63 [C63PI]) were detected in 59 isolates. C63PI, an iron transport system in SPI-1, was observed in 56% of the isolates (n = 46). SPI-1, SPI-4, and SPI-9 were present in 13, 2, and 5% of the isolates, respectively. The most common phenotypic resistances were observed to tetracycline (47%; n = 38), ampicillin (37%; n = 30), streptomycin (20%; n = 16), chloramphenicol (17%; n = 14), and trimethoprim-sulfamethoxazole (16%; n = 13). This study contributes to understanding the AMR genes present in S. enterica isolates from informal markets in Cambodia, as well as support domestic epidemiological investigations of multidrug resistance (MDR) profiles.

Winter Wheat Yield Response to Plant Density as a Function of Yield Environment and Tillering Potential: A Review and Field Studies.

Wheat (Triticum aestivum L.) grain yield response to plant density is inconsistent, and the mechanisms driving this response are unclear. A better understanding of the factors governing this relationship could improve plant density recommendations according to specific environmental and genetics characteristics. Therefore, the aims of this paper were to: i) execute a synthesis-analysis of existing literature related to yield-plant density relationship to provide an indication of the need for different agronomic optimum plant density (AOPD) in different yield environments (YEs), and ii) explore a data set of field research studies conducted in Kansas (USA) on yield response to plant density to determine the AOPD at different YEs, evaluate the effect of tillering potential (TP) on the AOPD, and explain changes in AOPD via variations in wheat yield components. Major findings of this study are: i) the synthesis-analysis portrayed new insights of differences in AOPD at varying YEs, reducing the AOPD as the attainable yield increases (with AOPD moving from 397 pl m-2 for the low YE to 191 pl m-2 for the high YE); ii) the field dataset confirmed the trend observed in the synthesis-analysis but expanded on the physiological mechanisms underpinning the yield response to plant density for wheat, mainly highlighting the following points: a) high TP reduces the AOPD mainly in high and low YEs, b) at canopy-scale, both final number of heads and kernels per square meter were the main factors improving yield response to plant density under high TP, c) under varying YEs, at per-plant-scale, a compensation between heads per plant and kernels per head was the main factor contributing to yield with different TP.

Gestantes atendidas no Setor de Doenças Sexualmente Transmissíveis da Universidade Federal Fluminense / Pregnants assisted at the Sexually Transmitted Disease Sector of the Universidade Federal Fluminense

Objetiva identificar os motivos que levam as gestantes a procurar atendimento no Setor de DST-UFF, definir os diagnósticos mais comuns nestas pacientes, identificar o perfil socioeconômico e sexuais das gestantes que procuram o Setor. Foi realizado um estudo retrospectivo, através de levantamento de todos os prontuários abertos no Setor entre 1995 e1998 - 4837 prontuários - destes foram selecionadas as pacientes que apresentavam um diagnóstico de gravidez - 157 pacientes, foram analisados vários dados para sabermos as características socioeconômicas e culturais, assim como dados do diagnóstico das pacientes. Apesquisa revelou que as gestantes formam parcela considerável da clientela do Setor DST-UFF, representando 5 por cento - 157/3.214 - do total de mulheres atendidas, notamos que 91 por cento das pacientes apresentavam alguma infecçäo genital, e que os diagnósticos mais freqüentes foram condiloma acuminado, seguido de vaginosebacteriana e sífilis, o local de domicílio foi Niterói, a faixa etária de 17 a 20 anos, estado civil solteira, escolaridade primeiro grau incompleto, renda até dois salários mínimos, e tinham apenas um parceiro. O motivo mais comum de procura aoSetor foi verrugas genitais, o diagnóstico mais freqüente foi condiloma acuminado, as pacientes näo tinham mais de um parceiro, a idae mais comum foi 17 anos, ea renda familiar de até dois salários mínimos