RESUMEN
PREMISE OF THE STUDY: Paspalum atratum is a perennial, cespitose, tropical grass native to Central and South America. This species belongs to a polyploid complex (Plicatula group) little known at the genetic level. The characterized microsatellite markers provide new informative tools for further studies of the hybridization, mating systems, and structure of the population. ⢠METHODS AND RESULTS: Using the microsatellite-enriched library method, we isolated and characterized 19 microsatellite markers from P. atratum. Eleven of them were polymorphic, showing a variable degree of variation, while eight were monomorphic in the samples analyzed. Additionally, the transferability of these microsatellite markers was tested in other species. ⢠CONCLUSIONS: These results suggest that the characterized markers have enough discriminatory potential to be used in genetic characterizations of Paspalum taxa, which are based on an understanding of their mating systems and genetic structure, as well as in understanding the evolutionary processes involved in the evolution of groups of Paspalum.
Asunto(s)
Humanos , Femenino , Embarazo , Recién Nacido , Citomegalovirus , Infecciones por Citomegalovirus/epidemiología , Estudios Seroepidemiológicos , Brasil , Infecciones por Citomegalovirus/diagnóstico , Infecciones por Citomegalovirus/congénito , Pruebas Serológicas , Complicaciones Infecciosas del Embarazo/etiologíaRESUMEN
Chromosome number, pairing relationship and meiotic behavior were evaluated in 24 Brazilian accessions of different Paspalum species as an initial screening to determine which of them might be useful in an interspecific hybridization program. The analysis showed that six were diploids, 16 tetraploids and two hexaploids. The pairing relationship was typical for the ploidy level and agreed with reported data. However, the meiotic behavior after diakinesis was much more abnormal than expected considering the pairing relationship. There was a high frequency of abnormal tetrads in the majority of accessions.
Asunto(s)
Cromosomas/ultraestructura , Genes de Plantas , Poaceae/genética , Brasil , Meiosis , Hibridación de Ácido Nucleico , PloidiasRESUMEN
O presente estudo visou estabelecer a prevalência do Citomegalovírus Humano (CMV) em gestantes e respectivos recém-nascidos, assistidos no Hospital Santa Juliana, no município amazônico de Rio Branco, Estado do Acre, período de agosto a novembro de 1998, através da Reaçäo Imunoenzimática com Final em Fluorescência (ELFA - Enzymelinked Fluorescent Assay) para CMV e avaliar possíveis fatores envolvidos na epidemiologia da doença. Tratou-se de um estudo soroepidemiológico, onde foram analisadas 607 amostras , 304 das mäes e 303 respectivos RNs, sendo dosadas CMV-IgM e CMV-IgG de ambos, gerando 1.214 dosagens para CMV. O diagnóstico laboratorial foi realizado no L.A.C. Dr. Luiz Augusto Batista também situado no mesmo município. A pesquisa de anticorpos IgG específicos foi positiva em 99,1 porcento das puérperas e de 99,66 porcento dos RNs. Quadro sorológico sugestivo de infecçäo primária gestacional por CMV foi de 0,66 porcento e de infecçäo congênita foi de 0,33 porcento