Determination of dysbiosis by the method of multiplex real-time polymerase chain reaction in chronic catarrhal gingivitis in children.

OBJECTIVE: Aim: The aim of the study was to determine the quantitative and qualitative characteristics of the microbiota of dento-gingival plaque in children to improve the quality of treatment of chronic catarrhal gingivitis. PATIENTS AND METHODS: Materials and Methods: It was examined 16 children aged 9-16 years with a diagnosis of K05.1: chronic gingivitis and 10 persons with intact gums were taken as a comparison group. A clinical dental examination was performed on the study participants and a sample was taken to determine the bacteria in the periodontal plaque. RESULTS: Results: The results of statistical processing of the research data allowed us to establish that in patients with chronic gingivitis, quantitative indicators of the total bacterial mass, Lactobacillus spp., Enterobacteriaceae, Gardnerella vaginalis/Prevotella bivia/Porphyromonas spp. in the sample of periodontal plaque significantly exceeded the indicators of healthy patients. It was determined that the examined children with chronic gingivitis, the total number of Lactobacillus spp. significantly exceeds its amount in people with intact gums. CONCLUSION: Conclusions: The changes in the quantitative and qualitative characteristics of the main representatives of the microf i lm of dento-gingival plaque, which characterize dysbiosis, are of signif i cant clinical signif i cance. Study of the quantitative characteristics of Lactobacterium spp., Enterobacterium spp., Streptococcacea spp., Gardnerella spp., Prevotella spp., Porphyromonas spp., Eubacteridacea spp., Mycoplasma (hominis + genitalium), Candida spp. is a diagnostic factor in determining the condition of the mucous membrane of the oral cavity.

CYTOLOGIC CHARACTERISTIC OF THE CELLULAR COMPOSITION OF THE GUM MUCOUS MEMBRANE IN SCHOOL-AGE CHILDREN.

OBJECTIVE: The aim: To establish the characteristics of the gingival mucosa cellular composition in school-age children. PATIENTS AND METHODS: Materials and methods: We made a dental and cytological examination on 150 Ukrainian (Poltava city) children aged 6, 12, and 15 years. Smears were got by scraping from the gum mucosa and stained with a May-Grunwald solution. Cytograms were made by using a Biorex-3 BM-500T microscope with a DCM-900 digital microphotographic attachment. The programs were adapted for these types of studies. We used a magnification of 1000. The t-test for paired samples was used to compare values. The difference was considered statistically significant at P<0.05. RESULTS: Results: The number of superficial epitheliocytes in children aged 12 and 15 was significantly higher than in 6-year-olds but did not differ from each other. CONCLUSION: Conclusions: The process of physiological keratinization of the cells of the gingival mucosa in schoolchildren is diverse and keratinization occurs both due to the phenomenon of physiological necrosis, namely, apoptosis of surface cells and due to orthokeratosis. From the age of 6, the average number of surface cells in cytograms decreases, while the number of intermediate and dead keratinized cells increases up to 12 years and remains on the previous level in children of 15 years.

AFFECTION ON CARIES AND ITS COMPLICATIONS OF TEMPORARY TEETH OF CHILDREN IN A REGION WITH EXCESS FLUORINE CONTENT IN DRINKING WATER.

OBJECTIVE: Aim: The aim of the study was to determine the indicators of caries and its complications in the temporary teeth of children who permanently live in a region with a high fluoride content in drinking water. PATIENTS AND METHODS: Materials and Methods: It was examined with the definition of caries and its complications 277 children in the age range from 2 to 13 years, who were born and permanently live in the urban-type settlement of Mashivka. The fluoride content in the drinking water of the settlement was 1.7-2.5 mg/l. RESULTS: Results: During the analysis of data from the survey of children who were born and permanently lived in the urban-type settlement of Mashivka, it was determined that the prevalence of caries of temporary teeth probably increases with age. Half of the 3-5-year-old children had caries-affected teeth, and temporary tooth caries reached the highest rates in 10-year-old children. It should be noted that a fifth of children in the youngest age group (3-5 years old) suffer from pulpitis and periodontitis of temporary teeth. CONCLUSION: Conclusions: The conducted examination of children urban-type settlement of Mashivka confirms the opinion that the excessive content of fluorine in drinking water does not have a caries-protective effect, and the intensity of the process reaches the indicators characteristic of regions with its optimal content. Such a situation requires strengthening measures for both primary and secondary prevention of dental diseases.

INFLUENCE OF MICROBIOTA ON THE CLINICAL AND IMMUNO-HISTOCHEMICAL CHARACTERISTICS OF CHRONIC GENERALIZED CATARRHAL GINGIVITIS IN CHILDREN.

OBJECTIVE: The aim: The purpose of the study is to characterize the influence of quantitative and qualitative composition of gingival microbiota on the status of the main immune system cells, localized in the gums, in chronic generalized catarrhal gingivitis in children. PATIENTS AND METHODS: Materials and methods: The study involved 26 children aged 9 to 16 years, patients with chronic generalized catarrhal gingivitis mild to moderate severity (CGCG) and 18 children with intact gums were comparison group. We determined the hygienic indices Fedorov, has been received, Silness-Loe, PMA, bleeding index for Myuleman and intensity of caries index CFD + cf, CFD. Histological and immunohistochemical studies were performed on serial sections kriostatnyh who made biopsy of gingival papillae. Microbiological study gingival part of crown plaque was performed by multiplexed PCR in real time. RESULTS: Results: Value hygienic indices in children with CGCG higher than in healthy, indicating the difficulty of care in the presence of periodontal inflammation. As a result of immunohistochemical studies revealed that HLA-DR + cells under conditions of active disease migrate to mucosal lamina propria epithelium. Number of CD3 + cells in the epithelium CGCG was significantly higher than the number in the intact epithelium and was the most numerous of population. In the biopsy of affected children significantly reduced the number of CD4 + cells. When CGCG quantitative total bacterial mass, Lactobacillus spp., Enterobacteriaceae, Gardnerella vaginalis / Prevotella bivia / Porphyromonas spp. in the sample CROWN dental plaque was significantly higher than rates under physiological conditions, and may serve as diagnostic criteria of dysbiosis. CONCLUSION: Conclusions: So, CGCG is a disease in the etiology of which is one of the leading roles played by microbial factor, namely, the value of the quantitative ratios of certain types of microorganisms of dental plaque compared to the total bacterial mass of plaque. Therefore, it is reasonable to include comprehensive treatment CGCG drugs in children, leading to natural immunostimulation which causes restoration of local immunity in the gum tissue and drugs to restore quantitative and qualitative composition of normal microflora of the child, thus providing a high therapeutic effect and serve as justification their choice.

Determination of the structure of acute injury of temporary and permanent frontal teeth in children, analysis of diagnostic and treatment measures and their effects.

OBJECTIVE: The aim: To determine the structure of acute injuries of temporary and permanent frontal teeth in children, to analyze the applied diagnostic and treatment measures for acute tooth trauma and to investigate their effectiveness. PATIENTS AND METHODS: Materials and methods: The subject of the study were 31 children aged 2-12 years with acute temporary and permanent tooth trauma. Methods: clinical (radiological, thermodiagnosis), medical and statistical. RESULTS: Results: The number of injured permanent teeth was greater than the number of injured temporary teeth. The pattern of temporary and permanent tooth injuries differed, so in temporary teeth dislocations were observed, and in permanent teeth fractures prevailed over dislocations. In our opinion, this is due to the peculiarities of the anatomy of the temporary teeth. Usually, the therapeutic tactics of acute temporary teeth injuries is to remove them, despite their important role in the growth and development of jaws, physiological formation and eruption of permanent teeth. The therapeutic tactics of dynamic observation in the case of intrusive dislocation of the temporary tooth were selected in the clinic of the Department of Pediatric Dentistry. In some cases, with a slight change in the position of the temporary tooth, self-regulation of its position was observed, in other cases the tooth remained dystopic, but its viability remained in 50% of cases. Therapeutic tactics of acute injuries of permanent teeth were selected according to the type of trauma. In the case of permanent tooth dislocation, with a slight change in its position, tooth immobilization was carried out by splinting with fiberglass tape and photopolymer composite material. CONCLUSION: Conclusions: Our observations have shown that the complex of modern specialized medical care for the affected children with acute traumatic injuries of the teeth should be guided by their preservation, which ensures the subsequent normal formation of the dental-jaw apparatus. To ensure these conditions, a long, reliable immobilization of the damaged tooth is required as soon as possible after injury.

Consideration of childhood psychological factors at dental appointment.

OBJECTIVE: Introduction: Paediatric dentistry is a special field of dental practice. The scope of professional activities within this field covers not only high level of expertise and technical skills tailored to the needs of young patients, but requires searching and creating positive psychological environment and communicative management of each child in order to improve daily service methods. The aim of this study is to identify and describe the main psychological characteristics of the age developmental periods of child's personality that dental professionals should regard dealing with the paediatric patients. PATIENTS AND METHODS: Materials and methods: The study involved 124 paediatric participants aged 2,5 - 15 years, who were referred to get specialized dental care at the Department of Paediatric Dentistry at the Municipal Paediatric Dentistry Hospital, Poltava. We have collected and identified behavioural patterns of children of all age groups during their contacts with dentists at the dental offices as well as studied the characteristics of childhood psyche age periods through the available literature. The data obtained were thoroughly As a result of the analysis of the obtained data, the principles for managing paediatric patients' behaviour at the dental office have been elaborated and introduced into the practice. RESULTS: Results: Outer evaluation of the children's activities performed is perceived by children as an assessment of personality, so any negative doctor's remark can provoke anxiety, irritability, and discomfort, apathy. Therefore, the paediatric specialists should remain attentive to physical and emotional indicators of stress when dealing with young patients. CONCLUSION: Conclusions: Hence, among the key tasks of a paediatric dentist is to create the friendliest and most comfortable conditions for small patients, where they have the opportunity to play toys and receive little gifts for patience and courage.

Studying the skills attitudes on factors affecting dental health of children.

OBJECTIVE: Introduction: Nowadays, the high level of dental morbidity among children is an urgent medical problem. Healthy lifestyles and sanitary-hygienic upbringing in the family is most important components of the formation of dental health of children. But in order to be able to teach their children, parents need to be themselves knowledgeable about hygiene and prevention, have the necessary skills and desire to instill it in their children. The aim: The aim of our study was to assess the level of awareness of schoolchildren parents about the factors affecting the dental health of their children according to the questionnaire. PATIENTS AND METHODS: Materials and methods: The article presents the results of a sociological survey in the form of anonymous polls on a specially designed questionnaire. With the help of the sociological method, 408 questionnaires, which were filled up by parents of students of general school №19 in Poltava, were processed and analyzed. RESULTS: Results: Among the interviewed individuals, we investigated groups of predictors that could increase the parents' awareness of the factors influencing the formation of health; to teach children by parents of the rules of personal hygiene of oral cavity and skills of self-prevention of dental diseases; on factors that lead to proper orthodontic pathology. CONCLUSION: Conclusions: Based on the parents' answers to the factors which form the health of the population, we can conclude that their awareness on this issue is insufficient. The results of the survey show insufficient level of knowledge in the issues of the formation of health and prevention of dental diseases among the respondents. Thus, the necessity to carry out the corresponding work in educational institutions with the involvement of teachers, dental doctors, pediatricians and doctors of other specialties is determined.