Air-dispersed aquatic microorganisms show establishment and growth preferences in different freshwater colonisation habitats.

We attempted to mimic aeolian ecosystems to examine how filters posed by regional characteristics can influence the establishment and growth of airborne microcolonisers of a common air source. Using a natural single source of aerosols we applied a combined microscopy and high-throughput sequencing approach to examine the diversity, settling and growth potential of air-dispersed microbes in water containers representing newly formed aquatic colonisation habitats of different trophic states and salinity. Heterotrophic microeukaryotes were favoured as initial settlers when nutrients were low, while autotrophs rapidly proliferated in the high-nutrient containers, possibly due to favourable germinating conditions for their preferred mode of dispersal with resting spores. Following settling of colonisers, we investigated two contrasting hypotheses: if the different water colonisation habitats harboured the same microbial communities after establishment and growth periods, this would point towards a selection of best-fit cosmopolitan colonisers, regardless of habitat-specific characteristics. Alternatively, community dissimilarities after the growth period would suggest a selection of settlers due to bottom-up controls combined with priority effects. Both analyses suggested that the structure of the microbial communities in the different colonisation habitats were driven by nutrient content and salinity, showing clustering to similar bottom-up forces and dissimilarities in significantly different colonisation habitats.

Quantitative ecotoxicological impacts of sewage treatment plant effluents on plankton productivity and assimilative capacity of rivers.

Sewage treatment plants are sources of inorganic and organic matter as well as contaminants for the receiving watercourses. We analyzed the ecological consequences of such effluents by following a holistic and synecological ecotoxicological approach based on quantifying extracellular enzyme activities (EEA), primary production and bacterial cell, and biomass production rates. Samples were obtained at three locations at the Rivers Holtemme and Elbe, Germany and Lower Jordan River, Israel and West Bank, as well as from their adjacent sewage treatment plants. Blending river samples with sewage treatment plant effluents mainly resulted in a stimulation of EEAs, which was diminished in blends with 0.2-µm filtered sewage treatment plant effluents. Stimulation for primary production and bacterial cell and biomass production of River Holtemme and Elbe samples was observed, and inhibition of these rates for Lower Jordan River samples probably linked to generally high turbidity. The quantified bacterial biomass versus cell production rates showed almost unbalanced (≫ 1) growth. Very high biomass to cell production ratios were found for sewage and sewage-containing samples, which provides a semi-quantitative indicator function for high quantities of microbial easy utilizable dissolved organic matter as nutrition source. The presented approach enables the simultaneous quantification of inhibitory and stimulating toxic responses as well as supplying ecosystem-based data for policy decision-making, and for direct incorporation in models to derive management and remediation strategies.