Molecular Evaluation and Identification of Some Barley Hybrids Tolerant to Salt Stress.

<b>Background and Objective:</b> Barley is considering one of the most important cereal crops at the local and global levels. It is ranked second in terms of nutritional importance after wheat and its flour contributes significantly to bridging the large nutritional gap in the production of Egyptian bread. The aim of this study concentrated on knowing and testing the genetic behaviour responsible for salinity stress tolerance in barley as trying to improve barley crop and increase its ability for abiotic stress resistance under Egyptian conditions. <b>Materials and Methods:</b> Twenty-one crosses and ten parents of barley with different responses to salinity tolerance were evaluated in this investigation under normal and salinity conditions. Yield and its components and some physiological traits related to salt stress tolerance were the most important studied attributes evaluated in this regard under both conditions. Moreover, SSR markers were used to evaluate and identified associated markers for salinity tolerance in selected hybrids and comparing among the ten barley parents. <b>Results:</b> The final results confirmed that the three testers; Giza 123, Giza 126 and Giza 2000 besides; the crosses; Line 1XTester 1 (Giza 125XGiza 123), Line 2XTester 1 (Giza 133XGiza 123), Line 1XTester 2 (Giza 125XGiza 126), Line 2XTester 2 (Giza 133XGiza 126) and Line 1XTester 3 (Giza 125XGiza 2000) exhibited highly salinity tolerance under saline stress treatment compared with the control experiment. Among 15 analyzed barley entries, the chosen set of 11 markers amplified 20 alleles with an average of 1.81, with a range from 1-4 alleles. <b>Conclusion:</b> The results of SSR analysis and the data on valued agricultural trait loci determined the genetic distance among parents and their hybrids, which is of an unlimited rate for breeders.

Stability Analysis and Molecular Description of Some Promising Sorghum Lines Tolerant to Salt Stress.

<b>Background and Objective:</b> Salt stress is considering the biggest environmental obstacle to crop productivity, especially sorghum. So, it was necessary to develop new sorghum lines tolerant to salt stress and high yielding to participate in bridging the large gap in the Egyptian bread industry and also as an important feed for animals. This is the biggest goalie this investigation. <b>Materials and Methods:</b> Some promising sorghum genotypes were evaluated under the control experiment and two salinity stress locations to test their stability and its salinity stress tolerance during two years. Some agro-morphological and physiological traits were the most important parameters tested under all conditions besides, 11 SCoT primers for comparing among the seven sorghum genotypes and Identification of molecular genetic markers responsible for salt stress tolerance. <b>Results:</b> The final results revealed that the five promising sorghum lines were recorded highly rank of salinity stress tolerance in all studied traits and a higher level of genetic stability during the two years. <b>Conclusion:</b> Results of agro-physiological traits, salinity tolerance indices and SCoT primers succeed in determining salt stress tolerance mechanisms in sorghum and which an important taxonomic tool is for plant breeder that helps him in sorting the tolerant genotypes from the sensitive ones.

Assessment of Water Stress Tolerance in Wheat Genotypes Based on Half Diallel Analysis and DNA Fingerprinting.

BACKGROUND AND OBJECTIVE: Deficit or scarcity of water resources are considering as one of the most serious environmental phenomenon that hinder agricultural production in many countries and the present study evaluated some wheat accessions for water stress tolerance under Egyptian conditions. MATERIAL AND METHODS: Five wheat genotypes and their ten F1 crosses from half diallel analysis were tested under both levels of irrigation (normal and drought stress conditions) through estimating some agro-morphological traits in addition, determining DNA fingerprinting meanwhile using five RAPD-PCR primers. RESULTS: Final results detected that 4 parents and the best 5 F1 crosses were the most desirable genotypes for water stress tolerance depending on all results obtained for all calculated parameters under normal and water deficit treatments. CONCLUSION: Traditional breeding and DNA fingerprinting could be used to clarify and sort all genotypes to generate the best of them for water stress resistance which will be in the future as a nucleus for producing resistance wheat varieties for drought stress under Egyptian conditions.