Diagnosis of human hydatidosis. Predictive value of a rural ultrasonographic survey in an apparently healthy population.

The usefulness of ultrasonography (US) in the early diagnosis of hydatidosis, applied in large-scale surveys to populations lacking clinical symptoms of the disease, has been amply documented. However, the rate of false positive and negative results is poorly described. Due to this, the present paper is aimed to evaluate the sensitivity, specificity and predictive value of a conventional rural ultrasonographic survey in comparison with higher imaging complexity. Accordingly, during 1997 and 1998 a total of 1054 children from 7 to 14 years of age were evaluated by means of US, in the town of Ingeniero Jacobacci, Province of Rio Negro, Argentina, employing a portable device for population studies. All detected cases were referred to a high complexity center specialized in imaging diagnosis for their re-evaluation with US, CT scanning and X-rays. A control group comprising 3 children negative by US for each positive case in the mass screening survey was selected and reexamined by US and X-rays and CT scanning in doubtful situations. Twenty-seven asymptomatic carriers were referred with images compatible with hydatid cysts, while 66 were classified as disease free. At reexamination, 24 of those diagnosed as carriers and the totality of those classified as healthy were confirmed. On the basis of our results, a sensitivity of 100%, a specificity of 95.6% and a global test value of 96.7% were estimated.

Diagnosis of human hydatidosis. Predictive value of a rural ultrasonographic survey in an apparently healthy population.

The usefulness of ultrasonography (US) in the early diagnosis of hydatidosis, applied in large-scale surveys to populations lacking clinical symptoms of the disease, has been amply documented. However, the rate of false positive and negative results is poorly described. Due to this, the present paper is aimed to evaluate the sensitivity, specificity and predictive value of a conventional rural ultrasonographic survey in comparison with higher imaging complexity. Accordingly, during 1997 and 1998 a total of 1054 children from 7 to 14 years of age were evaluated by means of US, in the town of Ingeniero Jacobacci, Province of Rio Negro, Argentina, employing a portable device for population studies. All detected cases were referred to a high complexity center specialized in imaging diagnosis for their re-evaluation with US, CT scanning and X-rays. A control group comprising 3 children negative by US for each positive case in the mass screening survey was selected and reexamined by US and X-rays and CT scanning in doubtful situations. Twenty-seven asymptomatic carriers were referred with images compatible with hydatid cysts, while 66 were classified as disease free. At reexamination, 24 of those diagnosed as carriers and the totality of those classified as healthy were confirmed. On the basis of our results, a sensitivity of 100

, a specificity of 95.6

and a global test value of 96.7

were estimated.

Nueva serovariedad mundial de Salmonella aislada de un caso de diarrea / A new serovar of Salmonella isolated from a case of diarrhea

Se informa el aislamiento por primera vez en el mundo, de una nueva serovariedad de Salmonella, a partir de heces de un niño con diarrea aguda en la ciudad de Orán, provincia de Salta. Por sus caracteres bioquímica fue obicada en la sub-especie IV, taxón 5, de acuerdo a la nomenclatura actual aceptada para el género, siendo su estructura antigénica 50:b:-(S IV 50:-)

Nueva serovariedad mundial de Salmonella aislada de un caso de diarrea / A new serovar of Salmonella isolated from a case of diarrhea

Se informa el aislamiento por primera vez en el mundo, de una nueva serovariedad de Salmonella, a partir de heces de un niño con diarrea aguda en la ciudad de Orán, provincia de Salta. Por sus caracteres bioquímica fue obicada en la sub-especie IV, taxón 5, de acuerdo a la nomenclatura actual aceptada para el género, siendo su estructura antigénica 50:b:-(S IV 50:-) (AU)

[New worldwide Salmonella serovar isolated in a case of diarrhea]. / Nueva serovariedad mundial de Salmonella aislada de un caso de diarrea.

The isolation of a new serovar of Salmonella from fecal material belonging to a child with acute diarrhea in the ville of Orán, province of Salta, is reported. Because of its biochemical characteristics it was classified within the sub-species IV, taxon 5, according to the present nomenclature for this genus, being its antigenic structure 50:b:-(S IV 50:b:-).

Nueva serovariedad mundial de Salmonella aislada de un caso de diarrea. / [New worldwide Salmonella serovar isolated in a case of diarrhea]

The isolation of a new serovar of Salmonella from fecal material belonging to a child with acute diarrhea in the ville of Orán, province of Salta, is reported. Because of its biochemical characteristics it was classified within the sub-species IV, taxon 5, according to the present nomenclature for this genus, being its antigenic structure 50:b:-(S IV 50:b:-).

[Isolation of new serovars of Salmonella in streams of water]. / Aislamiento de nuevas serovariedades de Salmonella en cursos de aguas.

The present work is the final stage of a research to determine the causes and degree of pollution of the Zaiman creek waters, which flow through the Capital District of Misiones, Argentina. The research was focused on the presence of Salmonella serovars in samples collected from 2 stream sites equidistant from a cold storage plant and slaughterhouse, one downstream, and the other before the source of pollution. Elevated temperature technique for the isolation of Salmonella was applied. The methodology consisted in applying an enrichment procedure for waterborne pathogens, performed by incubating after a convenient exposure period, gauze swabs in Dulcitol Selenite and Tetrationate (with the addition of 0,001% brilliant green) broths. Salmonella organisms were recovered by streaking duplicate plates of E.M.B Agar (Eosin Methylene Blue), B.P.L.S Agar (Brilliant Green Agar) and Bismuth Sulphite Agar. Best results were obtained when Bulcitol Selenite broth and Bismuth Sulphite Agar were used. (Tables 1, 2, 3). Suspected colonies were classified by 10 biochemical reactions, and further verified by serological identification. So far, 716 suspected colonies have been studied. Salmonella was isolated from 76% of the samples and 22 serovars were identified (Table 3). Isolating frequency of serovars during 1981-1984 is shown (Table 4). S. saphra prevailed among the studied samples, while both S. anatum and S. newport ranked second.(ABSTRACT TRUNCATED AT 250 WORDS)

Aislamiento de nuevas serovariedades de Salmonella en cursos de aguas / Isolation of new serum varieties of Salmonella in water streams

Se continuaron las investigaciones tendientes a determinar las causas y grado de contaminación de las aguas del arroyo Zaimán, del distrito Capital de la provincia de Misiones. Se investigó la presencia de serovars del género Salmonella, en muestras extraídas de 2 puntos equidistantes de un establecimiento frigorífico y matadero, en ambos sentidos de la corriente. Se aplicaron técnicas de incubación de alta temperatura, con plaqueos sucesivos efectuados a distintos tiempos de incubación. Como medios de enriquecimiento se emplearon Caldo-Dulcitol Selenito y Caldo-Tetrationato con Verde Brilhante al 0,001%. Para el aislamiento de colonias se utilizaron Agar Eosina-Azul de metileno, Agar Verde Brilhante con Sacarosa y Agar Sulfito de Bismuto. La mejor combinación resultó ser: Caldo Dulcitol Selenito-Agar Sulfito de bismuto. Se caracterizaron las cepas por medio de 10 reacciones bioquímicas y por serología. Se realizaron 34 operaciones de muestreo, hallando Salmonella en un 76% de las muestras analizadas, describiéndose un total de 22 serovariedades. Se señala a S. saphra como la serovar de mayor abundancia, ocupando S. anatum y S. newport el segundo lugar en forma compartida. La mayor parte de los aislamientos y serovars confirmados, provinieron del punto de muestreo ubicado aguas abajo del establecimento industrial, lo que demuestra la influencia de vertido de efluentes en el grado de contaminación de las aguas del arroyo Zaimán, considerándose que el nivel de Salmonella hallado constituye un riesgo potencial significativo para los probladores ribereños que utilizan estas aguas, principalmente con fines de recreación. Se destaca la detección de una nueva seroviedad en el orden mundial, designada como S. zaimán y la presencia de los primeros aislamientos en la Argentina de las serovars S. jos, S. madelia y S. belem, todas pertenecientes a la sub-especie I y la S. IV (HARMELEN) que corresponde a la sub-especie IV del género Salmonella (AU)

Aislamiento de nuevas serovariedades de Salmonella en cursos de aguas. / [Isolation of new serovars of Salmonella in streams of water]

The present work is the final stage of a research to determine the causes and degree of pollution of the Zaiman creek waters, which flow through the Capital District of Misiones, Argentina. The research was focused on the presence of Salmonella serovars in samples collected from 2 stream sites equidistant from a cold storage plant and slaughterhouse, one downstream, and the other before the source of pollution. Elevated temperature technique for the isolation of Salmonella was applied. The methodology consisted in applying an enrichment procedure for waterborne pathogens, performed by incubating after a convenient exposure period, gauze swabs in Dulcitol Selenite and Tetrationate (with the addition of 0,001

brilliant green) broths. Salmonella organisms were recovered by streaking duplicate plates of E.M.B Agar (Eosin Methylene Blue), B.P.L.S Agar (Brilliant Green Agar) and Bismuth Sulphite Agar. Best results were obtained when Bulcitol Selenite broth and Bismuth Sulphite Agar were used. (Tables 1, 2, 3). Suspected colonies were classified by 10 biochemical reactions, and further verified by serological identification. So far, 716 suspected colonies have been studied. Salmonella was isolated from 76

of the samples and 22 serovars were identified (Table 3). Isolating frequency of serovars during 1981-1984 is shown (Table 4). S. saphra prevailed among the studied samples, while both S. anatum and S. newport ranked second.(ABSTRACT TRUNCATED AT 250 WORDS)

Aislamiento de nuevas serovariedades de Salmonella en cursos de aguas / Isolation of new serum varieties of Salmonella in water streams

Se continuaron las investigaciones tendientes a determinar las causas y grado de contaminación de las aguas del arroyo Zaimán, del distrito Capital de la provincia de Misiones. Se investigó la presencia de serovars del género Salmonella, en muestras extraídas de 2 puntos equidistantes de un establecimiento frigorífico y matadero, en ambos sentidos de la corriente. Se aplicaron técnicas de incubación de alta temperatura, con plaqueos sucesivos efectuados a distintos tiempos de incubación. Como medios de enriquecimiento se emplearon Caldo-Dulcitol Selenito y Caldo-Tetrationato con Verde Brilhante al 0,001%. Para el aislamiento de colonias se utilizaron Agar Eosina-Azul de metileno, Agar Verde Brilhante con Sacarosa y Agar Sulfito de Bismuto. La mejor combinación resultó ser: Caldo Dulcitol Selenito-Agar Sulfito de bismuto. Se caracterizaron las cepas por medio de 10 reacciones bioquímicas y por serología. Se realizaron 34 operaciones de muestreo, hallando Salmonella en un 76% de las muestras analizadas, describiéndose un total de 22 serovariedades. Se señala a S. saphra como la serovar de mayor abundancia, ocupando S. anatum y S. newport el segundo lugar en forma compartida. La mayor parte de los aislamientos y serovars confirmados, provinieron del punto de muestreo ubicado aguas abajo del establecimento industrial, lo que demuestra la influencia de vertido de efluentes en el grado de contaminación de las aguas del arroyo Zaimán, considerándose que el nivel de Salmonella hallado constituye un riesgo potencial significativo para los probladores ribereños que utilizan estas aguas, principalmente con fines de recreación. Se destaca la detección de una nueva seroviedad en el orden mundial, designada como S. zaimán y la presencia de los primeros aislamientos en la Argentina de las serovars S. jos, S. madelia y S. belem, todas pertenecientes a la sub-especie I y la S. IV (HARMELEN) que corresponde a la sub-especie IV del género Salmonella