RESUMEN
A hybrid constructed wetland (CW) system with horizontal and vertical flow combination was evaluated for treating carbon-deficient synthetic dyeing wastewater containing 100 mg/L Reactive Yellow 145 dye. Organic waste products such as cow manure and wood chips were added as media in horizontal CW, and gravel as vertical CW media. Horizontal and vertical CWs were planted with Typha angustifolia. Horizontal CW was operated in continuous mode at hydraulic retention time (HRT) of 3 d and vertical CW in batch mode at 1 d HRT. The results suggested the potential application of a cost-effective horizontal-vertical hybrid CW to remove azo dyes from low-carbon dyeing wastewater. In horizontal CW, organic media was used as the carbon source for microbial dye degradation, resulting in 90% colour removal in the absence of available carbon in dyeing wastewater. Proteobacteria, Firmicutes and Bacteroidetes played a dominant role in dye degradation in horizontal CW. Vertical CW removed dye degradation organics, 69% ammonium-nitrogen and 39% organic-nitrogen. Phytotoxicity assays indicated toxicity reduction along the CW treatment path.