RESUMEN
OBJECTIVES: To assess in vitro the antimicrobial action of intracanal medicaments in the external root surface, with or without of cementum. METHODS: After the placement of intracanal medicaments, the coronal and apical openings were sealed. The antimicrobial action of 2% chlorhexidine gel (2%CHX); calcium hydroxide+2% chlorhexidine gel (CH+2%CHX); calcium hydroxide+2% chlorhexidine gel+zinc oxide (CH+2%CHX+ZnO); calcium hydroxide+saline solution (CH+SS) was evaluated against Enterococcus faecalis, Candida albicans, Actinomyces viscosus and Porphyromonas gingivalis. The samples were placed over the agar, and the zones of inhibition of microbial growth were measured after the incubation period. RESULTS: The antimicrobial effects of the medicaments could be ranked from strongest to weakest as follows: 2%CHX, CH+2%CHX, CH+2%CHX+ZnO, CH+SS. No differences among groups were verified in the presence or absence of cementum. CONCLUSIONS: 2%CHX containing medicaments diffused into the dentin and reached the outer surface, exerting antimicrobial action.
Asunto(s)
Antiinfecciosos/farmacología , Irrigantes del Conducto Radicular/farmacología , Raíz del Diente/microbiología , Actinomyces viscosus/efectos de los fármacos , Antiinfecciosos Locales/farmacología , Hidróxido de Calcio/farmacología , Candida albicans/efectos de los fármacos , Clorhexidina/farmacología , Recuento de Colonia Microbiana , Cemento Dental/microbiología , Dentina/microbiología , Enterococcus faecalis/efectos de los fármacos , Humanos , Ensayo de Materiales , Pruebas de Sensibilidad Microbiana , Porphyromonas gingivalis/efectos de los fármacos , Preparación del Conducto Radicular/instrumentación , Preparación del Conducto Radicular/métodos , Capa de Barro Dentinario , Óxido de Zinc/farmacologíaRESUMEN
The aim of this study was to investigate in vitro the antimicrobial activity of 0.2%, 1%, and 2% chlorhexidine gluconate (CHX gel and CHX liquid), against endodontic pathogens and compare the results with the ones achieved by 0.5%, 1%, 2.5%, 4%, and 5.25% sodium hypochlorite (NaOCl). A broth dilution test was performed, and the timing for irrigants to kill microbial cells was recorded and statistically analyzed. Both 2.0% gel and liquid formulations eliminated Staphylococcus aureus and Candida albicans in 15 seconds, whereas the gel formulation killed Enterococcus faecalis in 1 minute. All tested irrigants eliminated Porphyromonas endodontalis, Porphyromonas gingivalis, and Prevotella intermedia in 15 seconds. The timing required for 1.0% and 2.0% CHX liquid to eliminate all microorganisms was the same required for 5.25% NaOCl. The antimicrobial action is related to type, concentration, and presentation form of the irrigants as well as the microbial susceptibility.