[Role of helminth antigens in the abnormal mitosis of bone marrow cells in laboratory animals].

The intraabdominal administration of somatic extracts of the cestodes Hydatigera taeniaformis Batsch 1786, Lamarck, 1816 and Diphyllobothrium latum Linnaeus, 1758 and the nematodes Anisakis simplex larva Rudolphi 1809, Toxocara canis Railliet et Henry, 1912 in albino mice proved that these helminths had a karyopathic effect on the bone marrow cells of the animals. The antigenic composition of these extracts was investigated using the agar gel immunodiffusion test. The antigenic composition of the parasites was ascertained to affect their karyopathic properties. The amount of antigens and their foreignness caused a marked karyopathic effect on the bone marrow cells of laboratory animals during intraabdominal administration.

[Protective properties of the immunomodulator Gala-Vet in a complex with cellular Echinococcus multilocularis antigen in experimental hydatidosis].

The paper provides the results of studying the protective properties of the immunomodulator Gala-Vet alone and in combination with the cellular antigens of Echinococcus multiocularis protoscolexes in experimental murine hydatidosis. After checking inoculation with E. multilocularis protoscolexes and acephalocysts (a dose being 750+/-50 specimens), the protective efficiency in the mice immunized with the cellular antigen subcutaneously injected thrice in a dose of 60 micromg was 91.7%. In the mice immunized with the cellular antigen of parasitic protoscolexes in the same dose in the same manner, the protective effect was 75%. The similar protective effect (75%) was recorded in the group of mice receiving the immunomodulator Gala-Vet alone. After inoculation and slaughter, all the control mice administered a sterile physiological solution in the immunization period were infested.

[Comparative immunochemical characteristics and serological activity of the antigens of Cysticercus tenuicollis and Taenia hydatigena]. / Sravnitel'naia immunokhimicheskaia kharakteristika i serologicheskaia aktivnost' antigenov Cysticercus tenuicollis i Taenia hydatigena.

Data are given on immunochemical analysis and serological activity of different antigens from larval and imaginal forms of Taenia hydatigena. Considerable heterogeneity and close antigenic affinity of the parasite's extracts under study both between each other and with the host's proteins, excluding the antigens from T. hydatigena, which has no common components with the latter, are established. In the homologous system in each extract under study there were recognised no less than 5 to 9 antigenic components. It is shown, however, by the method of adsorption of heterologous antibodies that the number of specific antigens in each of them does not exceed 1 or 2. All antigens happened to be serologically active, but the highest diagnostic efficiency was shown by extracts from scolices of C. tenuicollis and T. hydatigena. Antiparasitic antibodies were followed by these antigens in the sera of experimentally infected sucking pigs from the 10th day of the infection. They reached their maximum level on the 24th day and then was observed a gradual fall of the titre of specific antibodies, the level of which by the 115th day did not actually differ from initial values. The highest sensitivity and specificity in the immunoenzyme reaction under experimental conditions was displayed by the extracts from scolices of C. tenuicollis.

[Antigenic affinity of cestodes in the genus Taenia]. / Antigennoe rodstvo tsestod roda Taenia.

Antigens of four species of the genus Taenia (T. ovis ovis, T. ovis krabbei, T. hydatigena and T. parenchimatosa) were studied by means of immunodiffusion reaction in agar gel with the use of hyperimmune sera. It has been established that extracts of the studied cestodes contain a great number of antigens, which during parenteral administration cause a synthesis of antibodies in rabbits. In homologous systems the number of recorded antigen-antibody complexes varied from 5 to 10. The most close antigenic affinity was found between T. ovis ovis and T. ovis krabbei, T. ovis ovis and T. hydatigena, as far as the main mass of precipitation bands in the immunodiffusion reaction fused together that suggests the identity of corresponding antigenic components. In all cases when analysing antiserum to T. parenchimatosa extract no differences of species-specific character in heterologous systems were traced.

[Dynamics of immunoglobulins and immunocompetent lymphocytes in guinea pigs with experimental ascariasis]. / Dinamika immunoglobulinov i immunokompetentnykh limfotsitov u morskikh svinok pri éksperimental'nom askaridoze.

The paper presents data on variations in the level of serous immunoglobulins (Ig M and Ig G classes), immunocompetent T- and B-lymphocytes in lymphoid organs (spleen, mesenteric, portal and mediastinal lymph nodes) and specific antiparasitic antibodies in guinea pigs during the dynamics of experimental ascariasis.