Complete Genome Sequence of a Gamaleyavirus Phage, Lytic against Avian Pathogenic Escherichia coli.

We report the complete genome sequence of the lytic bacteriophage vB_EcoS_Uz1, which was isolated from wastewater near Almaty, Kazakhstan using the avian pathogen Escherichia coli host. Its complete genome is 72,583 bp in length, with a GC content of 43%. vB_EcoS_Uz1 belongs to the Gamaleyavirus genus of the Caudoviricetes class.

Coding-Complete Genome Sequence of Swine Influenza Virus Isolate A/Swine/Karaganda/04/2020 (H1N1) from Kazakhstan.

Here, we report the coding-complete genome sequence of a clinical sample of influenza virus obtained from a pig at a livestock farm in Karaganda, Central Kazakhstan, during a pig study in 2020. Isolate A/Swine/Karaganda/04/2020 (H1N1) belongs to clade 1A.3.2.2 lineage 1A, which includes the 2009 H1N1 pandemic strains.

Effect of the nanocapsulated adjuvant Sapomax on the expression of some immune response genes.

The use of the nanocapsulated adjuvant Sapomax increased the expression of innate immunity genes (H2Q10, Ddx58, Tyk2, Tlr3, Tlr7, and TNF) responsible for the primary recognition of influenza virus, i.e., those belonging to the RLR and TLR families; genes involved in stimulating the production of type I and III IFN and pro-inflammatory cytokines; and Th1 and Th2 cellular immunity genes (Ccr4, Ccr5, IFNγ, IL-2, IL-4, and IL-10) responsible for triggering regulatory immune mechanisms in the cell. The high immunological activity of the plant-derived nanocapsulated adjuvant Sapomax may be used to enhance the efficacy of vaccines.

Metagenome Analysis of Surface Waters of the Shardara Reservoir, the Largest Artificial Reservoir in Southern Kazakhstan.

Here, we present a metagenomic analysis of the microflora of the surface waters of the Shardara reservoir, the largest artificial reservoir in Southern Kazakhstan, created to meet irrigation and hydropower engineering needs. In this case, shotgun metagenomic sequencing of the microbial community DNA was used.

Complete Genome Sequence of vB_EcoP_PR_Kaz2018, a T7-Like Bacteriophage.

Here, we describe the complete genome sequence of the Escherichia coli bacteriophage vB_EcoP_PR_Kaz2018, isolated from a water sample. vB_EcoP_PR_Kaz2018 is a linear double-stranded DNA T7-like podophage with a genome of 39,704 bp containing 45 predicted open reading frames (ORFs).

Complete Genome Sequence of Escherichia-Infecting Phage CEC_KAZ_2018, Isolated from Soil.

Avian pathogenic Escherichia coli (APEC) bacteria are one of the main problems of the poultry industry. An effective way to combat colibacillosis is to use a phage preparation that lyses the bacteria. Here, we report the isolation of an E. coli-infecting phage, CEC_KAZ_2018, isolated from soil.

Monitoring of Newcastle disease virus in environmental samples.

Newcastle disease virus (NDV) is an important pathogen in poultry. Waterfowl and a number of other avian species serve as the host for NDV. Severity of the disease is variable and infected animals mainly develop respiratory and neurological symptoms. Outbreaks of NDV in poultry are recorded regularly in the Republic of Kazakhstan despite the widespread use of vaccines. Here we present evidence that nucleic acid found in open water bodies in Kazakhstan can be detected by means of next-generation sequencing and belongs to at least three distinct genotypes of NDV.