[The effect of altered oxygen partial pressure on the resisitance to hypoxia and expression of oxygen-sensitive genes in Drosophila melanogaster].

As a result of resistance test to hypoxia of Drosophilas melanogaster of Oregon strain, we identified a high resistance (Group II) and low resistance (Group III) subpopulations of flies. Flies from groups II and III were incubated in a constant normobaric hypoxia (Po2=62-64 mm Hg) for 10 generations. A highly resistant group (Group IV) were exposed to a shortterm anoxia (Po,=1,5 mm Hg, 5 min) every generation. Larvae from Groups II, III, and IV demonstrated significantly elevated levels of Sir and CG 14740 expression. Larvae from Group II had a significantly higher expression of CG 14740 compared to group III. The restitution time after exposure to anoxia was significantly reduced in Group II (on 31% of the control values) Our results suggest that long-term adaptation to low oxygen partial pressure of highly resistant Drosophila significantly reduces the time of restitution and increases the expression of Sir2 and CG14740 genes.

[The effects of oxygen partial pressure changes on the osteometric markers of the bone tissue in rats].

Our purpose was to investigate the oxygen partial pressure changes on the osteometric and biochemical markers of bone tissue in rats. It was shown that breathing of altered gas mixture did not change the mass, general length, sagittal diameter and density thigh-bones in 12-month Wistar male-rats. The dosed normobaric hypoxia increased the activity of alkaline phosphatase and decreased the activity of tartrate-resistant acid phosphatase. At the same time normobaric hyperoxia with 40 and 90% oxygen conversely decreased the activity of alkaline phosphatase and increased the activity of tartrate-resistant acid phosphatase.

[Endothelium dependent vascular reactivity and autonomic homeostasis in children living in radiation-contaminated areas].

In order to study the mechanisms of development of pathological changes in children living in contaminated areas and chronically exposed to radionuclides as a result of exposure through the food chain, studied the vegetative homeostasis and thermographic study endothelium-dependent vascular reactions with occlusive tests. Showed signs of dysregulation of the autonomic nervous system dysfunction, the secretory activity of the endothelium and increased secretion of substances endothelial origin.

[Dosed normobaric hypoxia as a non-medication approach of stimulation of physiological regeneration of liver parenchyma].

The investigation was devoted to studying the effects of different modes of dosed normobaric hypoxia on the changes in morphometric indexes of functional activity and physiological regeneration of liver parenchyma in young rats. It was shown, that in rats exposed to hypoxic gas mixture (10% O2), the amount of karyonucleus and binuclear hepatocytes, nucleocytoplasmic and nucleolar-nuclear correlation increased. The changes in these indexes appeared to be more intensive when dosed normobaric intermittent hypoxia was administered with alternation of deoxygenation and reoxygenation periods. We believe that dosed normobaric hypoxia increases the functional activity and physiological regeneration of liver parenchyma.

[Normobaric hyperoxia affects osteogenesis in rats].

We studied the influence of normobaric hyperoxia on bone metabolism in 3- and 12-month-old male Wistar rats. Maintaining young rats (3 months) during the 14-hour daily sessions under normobaric hyperoxia (90% O2) was accompanied by a significant decrease in the concentration of C-terminal propeptides of collagen type I (by 36%), the acid phosphatase activity (by 32%), an increased activity of alkaline phosphatase (by 64%), an increased concentration of pyridinoline (by 37%) and 3-CrossLaps (by 8%), glycosaminoglycans (by 72%) in the blood serum. We believe that normobaric hyperoxia (90%) is accompanied by the disturbance of collagen synthesis. In adult rats under the same conditions for 14 sessions, the concentration ofglycosaminoglicans significantly increased by 60% relative to the control values. After 28 sessions of breathing the normobaric gas mixture containing 90% O 2 this parameter increased by 195%. Breathing normobaric gas mixture containing both 40% and 90% of O2 for 14 and 28 days decreased the acid phosphatase activity and the tartrat-resistant acid phosphatase activity by 18-25%. We believe that in adult animals 90% hyperoxia does not affect the activity of osteoblasts, but breaks the link between glycosaminoglicans and collagen fibrils, decreases the activity oflysosomal enzymes which are produced by osteoclasts and which can inhibit osteogenesis.

[Effect of normobaric hyperoxia on the markers of bone tissue metabolism].

We studied the influencing of normobaric hyperoxia one hour daily during 14 and 28 days on the bone metabolism for 3- and 12-monthly Wistar male-rats (n =80). It was shown, that breathed normobaric gas mixture with 90% O2 during 14 days significant reducing of C-terminal propeptides of type I collagen levels for 36%, an increasing piridinolin levels for 37% and concentration of C-terminal telopeptides of type I collagen for 8% in the blood serum. We found reliable decreasing the concentration ofpiridinolin levels for 27% in adult rats in the same conditions. The C-terminal propeptides of type I collagen levels did not change in any of the groups' research. We believe that 14 days normobaric hyperoxia (90%) in young animals accompanied by violation of collagen synthesis. The adult rats were more stable and had no changes after influence the two hyperoxia levels.

[Influence of food restriction on the biomechanical properties of the tibia in male rats of different age].

The changes of tibial bones biomechanical properties after 28 daily 40% declines of calorie content of feed and subsequent period of proceeding in a food ration were study in young and adult rats.28 day diminishing of calorie content of day's ration decline of bone mass, load capacity, stiffness, energy of elastic deformation of tibial bones. The indexes of biomechanics properties of bones were normalized after 28 daily period of proceeding in the ordinary ration of feed.

[Rheohepatography with ultrasound navigation of electrodes as biophysical method of the hepatic blood flow evaluation].

The transcutaneus Rheohepatography with ultrasound electrodes navigation (RUEN) discovered early specifically changes of hepatic blood flow in pts with diffuse hepatic pathology (DHP). It is necessary to use of the Conventional Rheohepatography for screening hepatics hemodynamics disorders in pts with DHP. For more differentiated approach it is necessary to use Doppler imaging of the hepatic blood flow. It is necessary to use the ultrasound electrodes navigation for increase of the self-descriptiveness Rheohepatography and for inclusion ofa maximum quantity hepatic parenchyma between interelectrodes space. As against the classical analysis extremities rheography in interpretation RUEN it is necessary to take into account, that the liver has 2 ways of inflow (proper hepatic artery and portal vein) and 1 way of outflow (hepatic veins running to cava inferior vein).

[The effect of preadaptation on the acute hypobaric stress and physiological bone tissue regeneration].

In experiments on rats we investigated the resistance to the barochamber testing (12000 u.s.l velositi 80 m/s) before and after training for 14 and 28 days in the normobaric gas mixture (N2 + O2, Po, = 100 +/- 10 mm of Hg). It was shown that after preadaptation the resistance to acute hypobaric hypoxia increased 1.7-1.8 times. Alimentary deprivation (40%) does not essentially affect the resistance to hypoxia. Biochemical markers show that normobaric low-dosed hypoxic preadaptation (14 days) activates the physiological regeneration process, promote the increase in concentrations of leptin, parathyreoid and growth hormones. Alimentary deprivation decreased an alkaline phosphatase activity and concentration of growth hormone. We suggest that normobaric hypoxic preadaptation but not alimentary limitation has definite positive influence on the bone tissue state.

[The effect of dosed normobaric hypoxia on physiologic regeneration of the liver parenchima in young and old rats].

We studied the effect of dosed normobaric hypoxia during 28 days on functional state and physiological regeneration of liver parenchyma. After completion of experiments in 3 and 9-month old rats we observed an increase in the amount of hepatocytes per unit of tissue area, with hypertrophied nucleus and decreased cytoplasmic area. IGF-I gene expression in 9-month old animals was increased. Thus, our study suggests that normobaric hypoxia stimulates physiological regeneration of liver parenchyma in young and adult rats.

[Effect of alimentary deprivation on physiological regeneration of the liver parenchyma in young and mature rats].

It was studied the effects of alimentary deprivation during 28 days on functional state and physiological regeneration of liver. In 3 and 9-month rats it was shown an increase in the amount of hepatocytes per unit of area of tissue, their nucleis were hypertrophied, the area of cytoplasm was decreased, the number of binucleitic hepatocytes in 9-month animals was increased. Thus, our study suggests that a short-term 40% food limitation stimulates physiological regeneration of liver parenchyma.

[Effect of dosed normobaric hypoxia on the bone tissue physiological regeneration].

We investigated the effect of dosed normobaric hypoxic gas mixtures with reduced oxygen partial pressure in the different modes on stimulation of the physiological bone tissue regeneration process in adult rats. It is shown that nitrogen and oxygen gas mixture (Po2 = 76 mm Hg) application in the modes 10 min deoxygenation--10 min reoxygenation during 4 hours daily or during 1 hour continuously is the optimum for an increase of biochemical markers of physiological regeneration of bone tissue.

[The principle of optimality in biophysical medicine].

It was consider biological, molecular, biochemical and clinical aspects of a problem of an optimality. Examples of selective sensitivity of alive formations in relation to temperature of environment, ultraviolet and infa-red radiations, the contents of light negative aeroions. The gas preferendum phenomenon at animals and the person in relation to oxygen partial pressure in an inhabitancy is in details analysed. The data on evolution of gas structure of atmospheric air and a role of oxygen radicals in physiological and pathological processes are resulted. It is stated the actual material testifying to presence enough of powerful mechanisms of the adaptation vertebrales animals, but the limited opportunity to resist to high concentration of oxygen. The conclusion is made, that conditions moderately reduced Po2 provide the maximal activity physiological regeneration and can recommended for wider use in practice of clinical and out-patient rehabilitation.

[Impedance testing of compact bone tissue in hypokinetic rats].

The bioelectrical impedance method was used for determination of compact bone status in white rats after 28th days of strong hypokinesia. It was shown that the lowering of mechanical loading leads to disturbances in dielectric properties and changes in electrical impedance parameters. These disturbances had different direction and manifestation. It was distinguished the two typical variants of bone dielectric properties changes. The first variant was more characteristic for early stages of hypokinetic osteodestruction, the second variant was more characteristic for the fully development hypokinetic disturbances. It was determine a correlation between the changes in electrical impedance parameters and mane components of bone matrix. The results of this study show that hyperhydratation of bone tissue play important role in hypokinetic changes of bone dielectric properties. Electroimpedance method may be used for early diagnostic of bone state in clinic and experiments.

[Electrophysiological study of tissue hydration in typical non-specific pulmonary pathology].

Computer occlusive electrical mioplethysmography and common electrical impedance meter method were used for the study of the tissue hydration in patients with bronchial asthma (BA) exposed to intermittent normobaric hypoxia (INH). Hyperhydration of intracellular and extracellular compartments in patients with BA was shown. INH significantly decreased tissue hyperhydration.

[The model of hypokinesia in rats and preventing its negative effects].

The special container for modeling of long-term hypokinesia state in rats was developed. Such construction allows us to examine simultaneously hypokinesia and its negative influence correction by gas mixture regulated with different oxygen partial pressure.

[The modeling of experimental osteopenia in rats and development of prophylaxis technology]. / Modeliuvannia eksperymental'noï osteopeniï ta rozrobka tekhnolohiï ïï profilaktyky u shchuriv.

An appliance for hind limb unloading in rats and the technology for preventing of inactivity osteopenia have been developed.

[Specific and non-specific electromagnetic irradiation effects on biological objects]. / Spetsyfichni ta nespetsyfichni efekty elektromahnitnykh vyprominiuvan' u biolohichnykh ob'éktakh.

There are the pecularities of the biophysical influence of the ultraviolet, light and infra-red irradiation in connection with their frequent and energetic characteristics. The specific resonant and non-specific heating effects are educed (distinguished). [table: see text] It is shown that the radial area of electromagnetic spectrum corresponding to the non-ionising. Sun irradiation, contains the evolutionary fixed molecular mechanisms of the energy acception activizing biochemical and biophysical metabolic reactions. The living beings, deprived of heliofugal influences (cave and deep-watered specimen objects) reached only the primitive development stages. The dosed wage of the non-ionising radiation generators in the clinic medicine promotes the restoration of the self sanogenic mechanisms and deficit restoration of the natural influences caused by the contemporary human being's mode of life changes.

[Infrared sweat secretion stimulation as a means of homeostatic correction in patients with kidney dysfunction]. / Infrachervona stymuliatsiia potovydilennia iak zasib korektsiï homeostazysu u osib z porushenniam nirkovoï diial'nosti.

The clinical and laboratory investigation was done on 40 patients with kidney disfunction (glomerulonefritis), treated by standard medicines with regulary infrared total body heating (from 15-20 to 40 min) daily during 10 days. Control group of 37 patients, was treated only by standard medicines. The thermochambers construction forces the possibility of normal temperature air breathing under the radial skin heating to 50-60 degrees C. It was shown, that hypertensions, dropsy manifestation and nitrogen contents in blood significantly decreased in comperison with the control group of patients. The positive effects in laboratory dates was shown in 65%; the subjective reports--in 100% patients. These data may be conformed to widley using infrared chamber procedures for combinative drugs and thermal treating patients with kidney disfunction.

[Effect of oxygen deprivation on the development of osteodystrophy caused by the load deficit]. / Vplyv kysnevoï depryvatsiï na rozvytok osteodystrofiï pry defitsyti navantazhennia.

The subject of the study was influence of gas mixture (GGS) with low Po2 on biomechanical properties of femoral and tibial bones in rats after hind-limb unloading. Experiments were performed in two different age groups (three month and six month) rats. It is shown that biomechanical properties of bone tissue in young rats, inhaling atmosferic air, under the influence of 28 days hind-limb unloading deteriorate essentially in comparison with adult rats. The biomechanical indexes of femoral and tibial bones in three month age rats close to control value after periodical inhaling of GGS. In six month age rats normalizing action of low Po2 not so essential. Thus bone tissue of young rats is more sensitive to different pathogenetic action than in adult rats. Such difference may be connected with retard of remodelation of bone tissue in adult rats.