First evidence of widespread positivity to anticoagulant rodenticides in grey wolves (Canis lupus).

Second-generation Anticoagulant Rodenticides (ARs) can be critical for carnivores, due to their widespread use and impacts. However, although many studies explored the impacts of ARs on small and mesocarnivores, none assessed the extent to which they could contaminate large carnivores in anthropized landscapes. We filled this gap by exploring spatiotemporal trends in grey wolf (Canis lupus) exposure to ARs in central and northern Italy, by subjecting a large sample of dead wolves (n = 186) to the LC-MS/MS method. Most wolves (n = 115/186, 61.8 %) tested positive for ARs (1 compound, n = 36; 2 compounds, n = 47; 3 compounds, n = 16; 4 or more compounds, n = 16). Bromadiolone, brodifacoum and difenacoum, were the most common compounds, with brodifacoum and bromadiolone being the ARs that co-occurred the most (n = 61). Both the probability of testing positive for multiple ARs and the concentration of brodifacoum, and bromadiolone in the liver, systematically increased in wolves that were found at more anthropized sites. Moreover, wolves became more likely to test positive for ARs through time, particularly after 2020. Our results underline that rodent control, based on ARs, increases the risks of unintentional poisoning of non-target wildlife. However, this risk does not only involve small and mesocarnivores, but also large carnivores at the top of the food chain, such as wolves. Therefore, rodent control is adding one further conservation threat to endangered large carnivores in anthropized landscapes of Europe, whose severity could increase over time and be far higher than previously thought. Large-scale monitoring schemes for ARs in European large carnivores should be devised as soon as possible.

Estimating Wolf Population Size and Dynamics by Field Monitoring and Demographic Models: Implications for Management and Conservation.

We estimated the current size and dynamics of the wolf population in Tuscany and investigated the trends and demographic drivers of population changes. Estimates were obtained by two different approaches: (i) mixed-technique field monitoring (from 2014 to 2016) that found the minimum observed pack number and estimated population size, and (ii) an individual-based model (run by Vortex software v. 10.3.8.0) with demographic inputs derived from a local intensive study area and historic data on population size. Field monitoring showed a minimum population size of 558 wolves (SE = 12.005) in 2016, with a density of 2.74 individuals/100 km2. The population model described an increasing trend with an average annual rate of increase λ = 1.075 (SE = 0.014), an estimated population size of about 882 individuals (SE = 9.397) in 2016, and a density of 4.29 wolves/100 km2. Previously published estimates of wolf population were as low as 56.2% compared to our field monitoring estimation and 34.6% in comparison to our model estimation. We conducted sensitivity tests to analyze the key parameters driving population changes based on juvenile and adult mortality rates, female breeding success, and litter size. Mortality rates played a major role in determining intrinsic growth rate changes, with adult mortality accounting for 62.5% of the total variance explained by the four parameters. Juvenile mortality was responsible for 35.8% of the variance, while female breeding success and litter size had weak or negligible effects. We concluded that reliable estimates of population abundance and a deeper understanding of the role of different demographic parameters in determining population dynamics are crucial to define and carry out appropriate conservation and management strategies to address human-wildlife conflicts.

A human-neutral large carnivore? No patterns in the body mass of gray wolves across a gradient of anthropization.

The gray wolf (Canis lupus) expanded its distribution in Europe over the last few decades. To better understand the extent to which wolves could re-occupy their historical range, it is important to test if anthropization can affect their fitness-related traits. After having accounted for ecologically relevant confounders, we assessed how anthropization influenced i) the growth of wolves during their first year of age (n = 53), ii) sexual dimorphism between male and female adult wolves (n = 121), in a sample of individuals that had been found dead in Italy between 1999 and 2021. Wolves in anthropized areas have a smaller overall variation in their body mass, during their first year of age. Because they already have slightly higher body weight at 3-5 months, possibly due to the availability of human-derived food sources. The difference in the body weight of adult females and males slightly increases with anthropization. However, this happens because of an increase in the body mass of males only, possibly due to sex-specific differences in dispersal and/or to "dispersal phenotypes". Anthropization in Italy does not seem to have any clear, nor large, effect on the body mass of wolves. As body mass is in turn linked to important processes, like survival and reproduction, our findings indicates that wolves could potentially re-occupy most of their historical range in Europe, as anthropized landscapes do not seem to constrain such of an important life-history trait. Wolf management could therefore be needed across vast spatial scales and in anthropized areas prone to social conflicts.

Use of European Funds and Ex Post Evaluation of Prevention Measures against Wolf Attacks (Canis lupus italicus) in the Emilia-Romagna Region (Italy).

INTRODUCTION: Compensation programs are an important tool for mitigating conflicts between farmers and large predators. However, they present significant weaknesses and faults. For years, the EU has been prioritizing programs for the prevention of damage caused by large carnivores, rather than compensation programs, introducing compulsory compensation for the purposes of decision EC (2019) 772 of 29/01/19. This manuscript reports the experience with the wolf damage prevention programs in an Italian region, Emilia-Romagna, which implemented a pilot project, adopting a new method to interface with the farmers involved in the prevention programs. METHODS: Starting in 2014, a project aimed at spreading prevention measures was financed through regional and European resources, accompanied by resources sharing and technical assistance with breeders from the regional body. In detail, (i) standardized types of intervention were defined and technical assistance was structured; (ii) ex post, the effectiveness of the interventions carried out was assessed; and (iii) the difficulties encountered in using the various financing instruments were analyzed. RESULTS: Overall, 298 farms were analyzed, of which 166 applied for regional calls and 132 applied for European funds. The mitigation measures produced a reduction in predatory phenomena of 93.4%, i.e., from 528 to 35 predations over a period of 4-6 years. This study shows that more than one-third of the farmers were forced to abandon the two tenders, mainly due to the lack of liquidity in anticipating the prevention measures. CONCLUSION: In the years examined by this study, the prevention programs in the Emilia-Romagna region, due to the technical support offered, proved to be a functional and effective tool, capable of significantly reducing the wolf predation on livestock. However, this work highlights the high percentage of denials of mitigation measures by farmers interested in adopting these tools, stressing the need for regional agencies to focus on new policies that can provide advance economic resources to farmers and solve the authorization problems related to the various bodies with which the participant in the tenders must interface.

Kleptoparasitism and Scavenging Can Stabilize Ecosystem Dynamics.

Scavenging is ubiquitous in nature, but its implications have rarely been investigated. We used camera traps on wolf kills to investigate the role of scavenging on predator and multiprey dynamics in a northern Apennine system in Italy. In contrast to North American systems, the omnivorous wild boar successfully competes with wolves for the meat of their kills. We developed a deterministic, multitrophic web model (wolf, vegetation, deer, and wild boar), tunable through a parameter that governs the impact of prey sharing between wolves and wild boar. When prey sharing is scarce, populations oscillate, but above a threshold value the trophic web is stabilized, with the regime solution becoming a fixed, stable point. Both deer and wild boar then increase as a function of prey sharing, and the impact of herbivores on the vegetation increases. When prey sharing exceeds another threshold, the system collapses due to the extinction of both wolves and wild boar. Our analysis shows that scavenging is crucial for the dynamics of this ecosystem, and thus it should not be overlooked in food web modeling. The exploitation of wolf kills by wild boar may allow juveniles and yearlings to obtain high-quality resources that are not usually available, helping the wild boar to compensate for losses caused by hunting. This is likely to make them even more invasive and difficult to control.