RESUMEN
In the current investigation, total phenolics and flavonoids of the methanolic extract obtained from the trunk bark of Acacia cyanophylla Lindl. were quantified by LC-HRMS technique. DPPH and ABTS reagents were employed to assay the antioxidant potential. The anti-tyrosinase and anti-α-amylase potentials were also assayed. The findings revealed that thirteen polyphenolic compounds were detected in the methanolic extract with trans-taxifolin (23.2â g/kg), as the major constituent. A. cyanophylla extract displayed a higher activity with DPPH test (IC50=10.14±1.00â µg/mL) than with ABTS (IC50=15.27±2.09â µg/mL). The same extract also exhibited interesting α-amylase inhibitory action (IC50 value of 4.00±0.17â µg/mL). Moreover, methanolic trunk bark extract exerted strong anti-tyrosinase capacity with an IC50 of 5.12±0.41â µg/mL in comparison to kojic acid (IC50=10.22±0.85â µg/mL) used as positive control. The antioxidant, anti-tyrosinase and anti-α-amylase potentials of the methanolic extract of A. cyanophylla trunk bark were reinforced by in silico molecular docking analyses, which confirmed the results of the inâ vitro tests.
RESUMEN
The aim of this study was to analyze the phytochemical profile of Acacia cyclops trunk bark ethyl acetate extract using LC-tandem mass spectrometry for the first time, along with evaluating its antioxidant and anti-tyrosinase properties. Consequently, we determined the total phenolic and flavonoid contents of the extract under investigation and identified and quantified 19 compounds, including phenolic acids and flavonoids. In addition to assessing their antioxidant potential against DPPH (2,2-diphenyl-1-picrylhydrazyl) and ABTS (2,2'-azino-bis-[3-ethylbenzothiazoline-6-sulfonic] acid) assays, in vitro and in silico studies were conducted to evaluate the tyrosinase inhibitory properties of the A. cyclops extract. The ethyl acetate trunk bark extract exhibited a substantial total phenolic content and demonstrated significant antioxidant activity in terms of free radical scavenging, as well as notable tyrosinase inhibitory action (half-maximal inhibitory concentration [IC50] = 14.08 ± 1.10 µg/mL). The substantial anti-tyrosinase activity of the examined extract was revealed through molecular docking analysis and druglikeness prediction of the main selected compounds. The findings suggest that A. cyclops extract holds promise as a potential treatment for skin hyperpigmentation disorders.
Asunto(s)
Acacia , Antioxidantes , Inhibidores Enzimáticos , Simulación del Acoplamiento Molecular , Monofenol Monooxigenasa , Corteza de la Planta , Extractos Vegetales , Monofenol Monooxigenasa/antagonistas & inhibidores , Acacia/química , Corteza de la Planta/química , Antioxidantes/farmacología , Antioxidantes/química , Antioxidantes/análisis , Extractos Vegetales/química , Extractos Vegetales/farmacología , Inhibidores Enzimáticos/química , Inhibidores Enzimáticos/farmacología , Inhibidores Enzimáticos/análisis , Fitoquímicos/química , Fitoquímicos/farmacología , Fitoquímicos/análisis , Espectrometría de Masas en Tándem/métodos , Flavonoides/química , Flavonoides/análisis , Flavonoides/farmacología , Fenoles/química , Fenoles/análisis , Fenoles/farmacología , Cromatografía Liquida/métodosRESUMEN
Two new phytochemical compounds, Tetranins A and B, 1-(3,5'-dihydroxy-4'-méthoxyphenyl)-2-phenylethanol 1 and 5,2'-dihydroxy-5'-methoxy-6,7-methylenedioxy-isoflavone 2, were isolated from the EtOAc extract of Salsola tetrandra roots. They exhibited a significant antioxidant effect in 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical and 2,2'-azinobis (3-ethylbenzothiazoline-6-sulphonic acid (ABTS) assays. Their structures were elucidated by extensive spectroscopic methods including 1-D-((1)H and (13)C) and 2-D-NMR experiments (CHcorr, HMBC, (1)H-(1)H COSY and NOESY) as well as high-resolution ES-MS and they were found to be quite active as antioxidants in the DPPH and ABTS assays.