Modified frankincense resin stabilized gold nanoparticles for enhanced antioxidant and synergetic activity in in-vitro anticancer studies.

For the first time, Frankincense resin (FR) has been carboxymethylated to produce CMFR - AuNPs and the conjugate was utilized for the Doxorubicin drug loading. The carboxymethylation of the carboxylic, phenolic, and hydroxyl functional groups of FR has been developed into carboxymethylated Frankincense resin (CMFR). A novel CMFR-AuNPs was synthesized using the developed CMFR as a stabilizing and reducing agent. The antibacterial, antioxidant, and in-vitro anticancer activities were investigated by using CMFR-AuNPs and CMFR - AuNPs@DOX. CMFR-AuNPs demonstrated antioxidative properties by quenching DPPH radicals effectively. CMFR-AuNPs and DOX@CMFR-AuNPs demonstrated strong antibacterial activity against K. pneumoniae, S. aureus, B. subtilis, and E. coli. The cell viability was tested for CMFR -AuNPs at various concentrations of Dox-loaded CMFR -AuNPs (CMFR-AuNPs + Dox1, CMFR-AuNPs + Dox 2, & CMFR-AuNPs + Dox 3). The highest inhibition was observed on MCF-7 and HeLa cell lines using CMFR-AuNPs + Dox 3, respectively. Various techniques such as UV, FTIR, TGA, XRD, SEM, EDAX and TEM were used to characterize the designed CMFR and CMFR-AuNPs. After carboxy methylation, the amorphous nature of FR changed to crystallinity, as reflected in the XRD spectra. The XRD spectrum of the CMFR- AuNPs showed FCC structure due to the involvement of hydroxyl and carboxylic functional groups of CMFR strongly bound with the AuNPs. TGA results revealed that the CMFR is thermally more stable than FR. TEM revealed that CMFR - AuNPs were well dispersed, spherical, and hexagonal with an average diameter of 7 to 10 nm, while the size of doxorubicin loaded (DOX@CMFR-AuNPs) AuNPs was 11 to 13 nm. Green CMFR-AuNPs have the potential to enhance the drug loading and anticancer efficacy of drugs.

Microwave-assisted synthesis of Limonia acidissima Groff gum stabilized palladium nanoparticles for colorimetric glucose sensing.

Herein, we present a novel microwave-assisted method for the synthesis of palladium nanoparticles (PdNPs) supported by Limonia acidissima Groff tree extract gum. The synthesized PdNPs were characterized using various analytical techniques, including FTIR, SEM, TEM, UV-visible, and powder XRD analyses. TEM and XRD analysis confirmed that the synthesized LAG-PdNPs are highly crystalline nature spherical shapes with an average size diameter of 7-9 nm. We employed these gum-capped PdNPs to investigate their peroxidase-like activity for colorimetric detection of hydrogen peroxide (H2O2) and glucose. The oxidation of 3,3',5,5'-tetramethylbenzidine (TMB) by H2O2, catalyzed by PdNPs, produces oxidation products quantified at 652 nm using spectrophotometry. The catalytic activity of PdNPs was optimized with respect to temperature and pH. The developed method exhibited a linear range of detection from 1 to 50 µm, with detection limits of 0.35 µm for H2O2 and 0.60 µm for glucose.

An efficient biosynthesis of palladium nanoparticles using Bael gum and evaluation of their catalytic and antibacterial activity.

We report a facile microwave-assisted synthesis of palladium nanoparticles (PdNPs) using Bael gum (BG) and it's carboxymethylated (CMBG) derivative. The prepared nanoparticles (BG@PdNPs and CMBG@PdNPs) were evaluated for antibacterial and catalytic activity in the reduction of organic dye pollutants. The developed synthetic method is simple, low cost and eco-friendly, wherein the process requires no additional reducing or capping agents. The CMBG was prepared via etherification reaction between BG and monochloroacetic acid using Williamson synthesis method. The PdNPs were synthesized using BG and CMBG as stabilizers and reducing agents. The PdNPs were found to be well dispersed spherical, with the crystalline size of the order of 7-21 nm. The results showed that the CMBG@PdNPs were smaller in size (7 ± 2 nm) than those capped with BG@PdNPs (10 ± 2 nm). The catalytic ability of CMBG@PdNPs was examined for the reduction of Methyl Orange (MO), Methyl Red(MR), and Rhodamine-B (RhB) in the presence of NaBH4. The results showed that CMBG@PdNPs exhibited a higher catalytic ability than BG@PdNPs. Moreover, it was found that CMBG@PdNPs served several times as a retrievable and reusable catalyst which is stable even after six cycles of reaction. The CMBG@PdNPs and BG@PdNPs showed excellent antibacterial activity. The results indicate that CMBG@PdNPs have greater potential application as a catalyst in the reduction of organic pollutants and antibacterial activity.