Overcoming memory T-cell responses for induction of delayed tolerance in nonhuman primates.

The presence of alloreactive memory T cells is a major barrier for induction of tolerance in primates. In theory, delaying conditioning for tolerance induction until after organ transplantation could further decrease the efficacy of the regimen, since preexisting alloreactive memory T cells might be stimulated by the transplanted organ. Here, we show that such "delayed tolerance" can be induced in nonhuman primates through the mixed chimerism approach, if specific modifications to overcome/avoid donor-specific memory T-cell responses are provided. These modifications include adequate depletion of CD8+ memory T cells and timing of donor bone marrow administration to minimize levels of proinflammatory cytokines. Using this modified approach, mixed chimerism was induced successfully in 11 of 13 recipients of previously placed renal allografts and long-term survival without immunosuppression could be achieved in at least 6 of these 11 animals.

Blood groups in relation to carcinoma of cervix uteri.

Data are presented on AB0 and RhD blood groups in 186 patients suffering from carcinoma of cervix uteri and 274 controls from Delhi, India. A strong association is observed between carcinoma patients and blood group A, and a slightly weaker association with blood group B. There is no significant association with RhD blood group. The available data in other populations confirm the association with AB0 blood group.

Fertility and mortality differentials among the different population groups of Sikkim, India.

PIP: Differential mortality and fertility trends among the Buddhist and Hindu populations of the Indian state of Sikkim are analyzed using data from a sample of 281 mothers who had completed their reproductive cycles. The relative importance of the fertility and mortality components in the natural selection process among the populations studied is discussed.^ieng

Biology of the people of Sikkim, India. 2. Colour blindness, ear lobe attachment, mid-phalangeal hair and behavioural traits.

14 population groups of Sikkim (India)--Lepchas (2), Bhutias (2), Sherpas, Tamangs, Gurungs, Mangars, Rais, Limboos/Subbas, Pradhans (Newars), Brahmans, Chhetris, Scheduled Castes--have been studied in regard of the intra- and intergroup variability of colour blindness, ear lobe attachment, mid-phalangeal hair and behavioural traits (tongue folding, hand clapsing, arm folding, leg folding, handedness). Some of these variables show a considerable distribution heterogeneity, which is discussed considering history and marriage patterns of these populations. As most of them are highly endogamous one can assume that this heterogeneity is caused by locally acting factors such as drift and/or founder effects, which could be preserved due to as good as lacking gene flow among the populations under study. Beyond that the Sikkim data are compared briefly with those reported for other Indian and Asiatic populations.

Y-chromosome length heteromorphisms in Delhi infants.

One hundred and seventy normal male infants from Delhi were studied using the CBG technique to estimate Y-chromosome length heteromorphisms. The median class in Y/F [Y/F = total length of the Y chromosome/average total length of the F group chromosomes (19 and 20)] distribution was 0.75-0.79. The Y/F index in infants varied from 0.60 to 1.16 with a mean of 0.81 and a standard deviation of 0.09. A high incidence for very small (53.5 percent) and small (41.2 percent) categories of Y-chromosome length heteromorphisms was observed. Data were compared with other available reports; also possible mechanisms of the Y-chromosome length heteromorphisms and their role in ethnic/racial variation as well as in developmental disturbances are discussed. It is suggested there may be a need to redefine the long and short Y chromosome in a given population while studying different clinical disorders.

Genetic studies of Pangwalas, Transhumant and Settled Gaddis. 4. Colour blindness, mid-phalangeal hair, ear lobe attachment and behavioural traits.

Frequency distributions of colour blindness, midphalangeal hair, ear lobe attachment, hand clasping, arm folding, leg folding and handedness are reported for different population groups from Himachal Pradesh, North India, namely Pangwalas, Transhumant Gaddis (Brahmans, Rajputs and Scheduled Castes) and Settled Gaddis (Brahmans, Rajputs and Schedules Castes). An attempt has been made to compare the results of the present study within and between these groups as well as with the results of other reports from different population groups of India and Asia.

Genetic markers and malaria. Observations in Gujarat, India.

189 healthy controls and 175 patients suffering from malaria vivax have been investigated with regard to associations between this disease and 22 genetic polymorphisms of the blood (ABO, MN, Ss, Rh, Kell, P, Lutheran, Kidd, Duffy, Diego, Xg; ABH-Secretor; Hp, Gc, Gm, Km; aP, AK, PGM1, 6-PGD, EsD; Hb variants) Significant associations could be demonstrated only for P and Hp systems, though in accordance with other investigations it cannot be excluded that the ABO system plays also a role in this connection.

Human chromosomal heteromorphisms in Delhi newborns. IV. Inter-relationship between C-band size and inversion heteromorphisms.

The inter-relationship of C-band size and inversion heteromorphisms was studied in 200 Delhi normal newborns (100 males and 100 females). A significant correlation between size and inversion heteromorphisms in males (r = 0.97) and females (r = 0.98) was observed. The results suggested that the larger the size of the C-band regions so the higher was the incidence of inversion.

Chromosomal heteromorphisms in Delhi infants. III. Qualitative analysis of C-band inversion heteromorphisms of chromosomes 1, 9, and 16.

Qualitative analysis of C-band heteromorphisms was carried out in 200 infants (100 males and 100 females) in Delhi, India. Partial inversions minor and half inversions were observed as modal levels for chromosomes 1 and 9 in both sexes. No chromosome 16 with a C-band inversion was observed in the present investigation. A significantly higher incidence of percent inversions for chromosomes 1 and 9 was observed in males than in females. The frequency of heterozygous inversion level combinations for chromosome pairs 1 and 9 were remarkably higher than homozygous combinations both in males and females. Our results are compared with the other reported studies, and the possible role of these heteromorphisms in ethnic/racial variation and in developmental disturbances are discussed.

Human chromosomal heteromorphisms in Delhi newborns. II. Analysis of C-band size heteromorphisms in chromosomes 1, 9 and 16.

Quantitative analysis of C-band size heteromorphisms in chromosomes 1,9 and 16 was carried out in 200 Delhi newborns (100 males and 100 females). The percent size heteromorphisms for chromosomes 1,9 and 16 showed nonsignificant differences between the sexes. Homozygous size level combinations showed higher incidence than the heterozygous combinations for all the three chromosome pairs studied in both sexes. Our results are compared with other reported studies and the possible role of these heteromorphisms in ethnic/racial variation and in developmental disturbances is discussed.

Genetic studies in four tribal populations of the Surat District, Gujarat (India).

Four tribal populations (Chaudhuri, Vasava, Kotwalia and Gamit) of the Surat District in Gujarat (India) have been investigated for the distribution of 22 polymorphic systems of the blood. The main results of this study are as follows: The allele frequencies show considerable heterogeneity among these populations. From the genetic structure analysis it is seen that only a small fraction of the total gene diversity accounts for genetic differences among them, and the major portion of it is due to genetic variation within them. Analysis of genetic distance according to Nei (1972) reveals that the Vasava and Kotwalia show a rather close genetic relationship, while the Chaudhuri and Gamit differ from both the Vasava and Kotwalia.

Transferrin subtypes in four Northwest Indian tribal populations and some remarks on the anthropological value of this new polymorphism.

Serum samples from four tribal Populations--Chaudhuri, Kotwalia, Vasava, Gamit-living in Gujarat (Northwest India) were tested for transferrin subtypes. The results are compared with those reported for other Indian populations. Reviewing all the hitherto published Tf subtype data some interesting racial differences in the distribution of gene frequencies emerge. A possible geographical distribution pattern of the variants is explored.

Anthropological studies among Pangwalas and Gaddis of Himachal Pradesh, North India.

The present paper is based on the Research Project entitled "Impact of Technological and Economic Development Programme on Bio-Cultural Heritage of Gaddis and Pangwalas Population Groups of Himachal Pradesh". The project was framed after UNESCO/MAB Project Nos. 6 and 12 under UNESCO Programme on Man Biosphere (MAB) and supported by the Department of Science and Technology, Government of India. The population groups under investigation are: 1. Transhumant Gaddis of Bharmour Sub-Tehsil, Chamba District; 2. Settled Gaddis of Kangra District; and 3. Pangwalas of Pangi Tehsil, Chamba District, Himachal Pradesh. The carrying capacity has been calculated for Pangi and Bharmour Sub-Tehsil and it has been observed that in both the places the size of population is beyond the capacity of the land. The pattern of body growth and respiratory functions among Transhumant Gaddis of Bharmour Sub-Tehsil, Chamba District and Settled Gaddis of Kangra District have been studied. Statistically non-significant differences in most of the biological variables have been observed among these groups.

Genetic diversity among the Newars of Nepal.

Genetic diversity among the Newars of Nepal has been studied using Wright's FST and the ratio of observed variance to theoretical variance following Lewontin and Krakauer's 1973 method, based on six genetic characteristics. The gene differentiation among the Newars is only 1.7%. These observations are further corroborated by the results obtained through genetic distance analysis. The average heterozygosity per locus is high (ranging from 35 to 42%) for all the groups. About 95% of total gene diversity exists within the Newar groups, the intergroup components being only about 5%.