Vis-NIR spectroscopy based rapid and non-destructive method to quantitate microplastics: An emerging contaminant in farm soil.

Microplastics (MPs) is the second most important environmental issue and can potentially enter into food chain through farmland contamination and other means. There are no standardized extraction methods for quantification of MPs in soil. The embedded errors and biases generated serious problems regarding the comparability of different studies and leading to erroneous estimation. To address this gap, present study was formulated to develop an efficient method for MPs analysis suitable for a wide range of soil and organic matrices. A method based on Vis-NIR (Visible-Near Infra Red) spectroscopy is developed for four different soil belonging to Alfisol, Inceptisol, Mollisol and Vertisol and two organic matter matrices (FYM and Sludge). The developed method was found as rapid, reproducible, non-destructive and accurate method for estimation of all three-density groups of MPs (Low, Medium and High) with a prediction accuracy ranging from 1.9 g MPs/kg soil (Vertisol) to 3.7 g MPs/kg soil (Alfisol). Two different regression models [Partial Least Square Regression (PLSR) and Principal Component Regression (PCR)] were assessed and PLSR was found to provide better information in terms of prediction accuracy and minimum quantification limit (MQL). However, PCR performed better for organic matter matrices than PLSR. The method avoids any complicated sample preparation steps except drying and sieving thus saving time and acquisition of reflectance spectrum for single sample is possible within 18 s. Owing to have the minimum quantification limit ranging from 1.9-3.7 g/kg soil, the vis-NIR based method is perfectly suitable for estimation of MPs in soil samples collected from plastic pollution hotspots like landfill sites, regular based sludge amended farm soils. Additionally, the method can be adapted by small scale compost industries for assessing MPs load in product like city compost which are applied at agricultural fields and will be helpful in quantifying possible MPs at the sources itself.

Reducing the environmental impact of rice production in subtropical India by minimising reactive nitrogen loss.

The future of reactive nitrogen (N) for subtropical lowland rice to be characterised under diverse N-management to develop adequate sustainable practices. It is a challenge to increase the efficiency of N use in lowland rice, as N can be lost in various ways, e.g., through nitrous oxide (N2O) or dinitrogen (N2) emissions, ammonia (NH3) volatilization and nitrate (NO3-) leaching. A field study was carried out in the subsequent wet (2021) and dry (2022) seasons to assess the impacts of different N management strategies on yield, N use efficiency and different N losses in a double-cropped rice system. Seven different N-management practices including application of chemical fertilisers, liquid organic fertiliser, nitrification inhibitors, organic nutrient management and integrated nutrient management (INM) were studied. The application of soil test-based neem-coated urea (NCU) during the wet season resulted in the highest economic yield, while integrated nutrient management showed the highest economic yield during the dry season. Total N losses by volatilization of NH3, N2O loss and leaching were 0.06-4.73, 0.32-2.14 and 0.25-1.93 kg ha-1, corresponding to 0.06-5.84%, 0.11-2.20% and 0.09-1.81% of total applied N, respectively. The total N-uptake in grain and straw was highest in INM (87-89% over control) followed by the soil test-based NCU (77-82% over control). In comparison, recovery efficiency of N was maximum from application of NCU + dicyandiamide during both the seasons. The N footprint of paddy rice ranged 0.46-2.01 kg N-eq. t-1 during both seasons under various N management. Ammonia volatilization was the process responsible for the largest N loss, followed by N2O emissions, and NO3- leaching in these subtropical lowland rice fields. After ranking the different N management practices on a scale of 1-7, soil test-based NCU was considered the best N management approach in the wet year 2021, while INM scored the best in the dry year 2022.

Above and below-ground growth, accumulated dry matter and nitrogen remobilization of wheat (Triticum aestivum) genotypes grown in PVC tubes under well- and deficit-watered conditions.

The continuing decline in water resources under the ever-changing climate compels us to re-orient our focus to a more sustainable practice. This study investigates the performance of Triticum aestivum wheat genotypes viz. HD-2967, HD-3086, HD-3249, DBW-187, and HD-3226 under well- and deficit-watered conditions for their root-traits, biomass and nitrogen accumulation and remobilization, and water use efficiencies, grown in PVC-tubes. The genotypes HD-2967, HD-3086, HD-3249, DBW-187, and HD-3226 under well-watered (WW) resulted in 36, 35, 38, 33, and 42% more grain yield compared to deficit-watered (DW). Among the genotypes, HD-3249 had the highest grain yield under both well- and deficit-watered conditions. Compared to DW, the WW had 28%, 30%, and 28% greater root length, biomass, and root length density at flowering {102 days (d), Z61}, while among the genotypes, HD-3249 had relatively greater root-traits. At flowering (Z61) and maturity (132 d, Z89), genotypes under WW accumulated 30-46% and 30-53%, respectively greater shoot biomass over the DW. Furthermore, the shoot biomass remobilised for HD-2967, HD-3086, HD-3249, DBW-187, and HD-3226 under the WW was 32, 37, 39, 35, and 35% greater than the DW. The nitrogen partitioning to different plant parts at flowering (Z61) and maturity (Z89) was significantly greater with the WW than with DW. The total nitrogen- remobilized and contribution to grain-N under the WW was 55, 58, 52, 53, 58% and 9, 19, 15, 17, 17% greater than the DW for the genotypes HD-2967, HD-3086, HD-3249, DBW-187, and HD-3226. The irrigation water use efficiency (WUE) at flowering (Z61) was more under the deficit-watered, but the biomass and grain total WUE was improved with the well-watered condition. Hence, it is apparent that proper scheduling of irrigation and N applications, along with the adoption of a genotype suited to a particular environment, will result in better WUE and grain yields, along with better utilization of scarce resources.

Timely sown maize hybrids improve the post-anthesis dry matter accumulation, nutrient acquisition and crop productivity.

Delayed sowing of maize hybrids could exacerbate the capability of maximizing the yield potential through poor crop stand, root proliferation, nutrient uptake, and dry matter accumulation coupled with the inadequate partitioning of the assimilates. This study appraised the performance of five recent maize hybrids viz., PMH-1, PJHM-1, AH-4158, AH-4271, and AH-8181 under timely and late sown conditions of the irrigated semi-arid ecologies. Timely sowing had the grain and stover yields advantage of 16-19% and 12-25%, respectively over the late sown maize hybrids. The advanced hybrids AH-4271 and AH-4158 had higher grain yields than the others. During the post-anthesis period, a greater dry matter accumulation and contribution to the grain yield to the tune of 16% and 10.2%, respectively, was observed under timely sown conditions. Furthermore, the nutrient acquisition and use efficiencies also improved under the timely sown. The nutrient and dry matter remobilization varied among the hybrids with AH-4271 and PMH-1 registering greater values. The grain yield stability index (0.85) was highest with AH-4158 apart from the least yield reduction (15.2%) and stress susceptibility index (0.81), while the maximum geometric mean productivity was recorded with the AH-4271 (5.46 Mg ha-1). The hybrids AH-4271 and PJHM-1 exhibited improved root morphological traits, such as root length, biomass, root length density, root volume at the V5 stage (20 days after sowing, DAS) and 50% flowering (53 DAS). It is thus evident that the timely sowing and appropriate hybrids based on stress tolerance indices resulted in greater yields and better utilization of resources.

Cultivar assortment index (CAI): a tool to evaluate the ozone tolerance of Indian Amaranth (Amaranthus hypochondriacus L.) cultivars.

The adverse impact of climate change on crop yield has accelerated the need for identification of crop cultivars resistant to abiotic stress. In the present study, a cultivar assortment index (CAI) was generated for the evaluation of forty Amaranthus hypochondriacus cultivars response to elevated ozone (EO) concentrations (AO + 30 ppb) in Free Air Ozone Enrichment (FAOE) facility using the parameters viz. foliar injury, gaseous exchange attributes, namely, net photosynthetic rate, stomatal conductance, transpiration rate, intercellular carbon dioxide, and water use efficiency along with above ground biomass and grain yield attributes. The dataset was used to identify key indicator parameters responsive to EO through principal component analysis (PCA) and further transformed to obtain linear score and weighted score. The CAI varied from 70.49 to 193.43. Cultivars having CAI value less than 151 were ozone tolerant (OT) whereas cultivars with CAI values between 150 and 170 were moderately tolerant (MOT). The cultivars exhibiting CAI values above 170 were ozone sensitive (OS). The cultivars exhibited differential sensitivity to EO with IC-5994 (CAI = 187.26) being the most affected cultivar whereas IC-5576 (CAI = 83.38) and IC-5916 (CAI = 70.49) being the least affected ones. The CAI, based on linear score and weighted score, offers easy identification of ozone sensitive (OS) and ozone tolerant (OT) cultivars. This index could help researchers to define a clear and strong basis for identification of OT cultivars which will reduce the time required for preliminary screening and further evaluation of crop cultivars for the development of climate smart crops.

How Modelers Model: the Overlooked Social and Human Dimensions in Model Intercomparison Studies.

There is a growing realization that the complexity of model ensemble studies depends not only on the models used but also on the experience and approach used by modelers to calibrate and validate results, which remain a source of uncertainty. Here, we applied a multi-criteria decision-making method to investigate the rationale applied by modelers in a model ensemble study where 12 process-based different biogeochemical model types were compared across five successive calibration stages. The modelers shared a common level of agreement about the importance of the variables used to initialize their models for calibration. However, we found inconsistency among modelers when judging the importance of input variables across different calibration stages. The level of subjective weighting attributed by modelers to calibration data decreased sequentially as the extent and number of variables provided increased. In this context, the perceived importance attributed to variables such as the fertilization rate, irrigation regime, soil texture, pH, and initial levels of soil organic carbon and nitrogen stocks was statistically different when classified according to model types. The importance attributed to input variables such as experimental duration, gross primary production, and net ecosystem exchange varied significantly according to the length of the modeler's experience. We argue that the gradual access to input data across the five calibration stages negatively influenced the consistency of the interpretations made by the modelers, with cognitive bias in "trial-and-error" calibration routines. Our study highlights that overlooking human and social attributes is critical in the outcomes of modeling and model intercomparison studies. While complexity of the processes captured in the model algorithms and parameterization is important, we contend that (1) the modeler's assumptions on the extent to which parameters should be altered and (2) modeler perceptions of the importance of model parameters are just as critical in obtaining a quality model calibration as numerical or analytical details.

Influence of cyanobacterial inoculants, elevated carbon dioxide, and temperature on plant and soil nitrogen in soybean.

Climate change affects nitrogen dynamics in crops and diazotrophic microorganisms with carbon dioxide (CO2 ) sequestering potential such as cyanobacteria can be promising options. The interactions of three cyanobacterial formulations (Anabaena laxa, Calothrix elenkinii and Anabaena torulosa-Bradyrhizobium japonicum biofilm) on plant and soil nitrogen in soybean, were investigated under elevated CO2 and temperature conditions. Soybean plants were grown inside Open Top Chambers under ambient and elevated (550 ± 25 ppm) CO2 concentrations and elevated temperature (+2.5-2.8°C). Interactive effect of elevated CO2 and cyanobacterial inoculation through A. laxa and Anabaena torulosa-B. japonicum biofilm led to improved growth, yield, nodulation, nitrogen fixation, and seed N in soybean crop. Nitrogenase activity in nodules increased in A. laxa and biofilm treatments, with an increase of 55% and 72%, respectively, over no cyanobacterial inoculation treatment. Although high temperature alone reduced soil microbial biomass carbon, dehydrogenase activity, and soil available N, the combined effect of CO2 and temperature were stimulatory; cyanobacterial inoculation further led to an increase under all the conditions. The highest seed N uptake (758 mg plant-1 ) was recorded with cyanobacterial biofilm inoculation under elevated CO2 with control temperature conditions. The positive interactions of elevated CO2 and cyanobacterial inoculation, particularly through A. laxa and A. torulosa-B. japonicum biofilm inoculation highlights their potential in counteracting the negative impact of changing climate along with enhancing plant and soil N in soybean.

Appraisal of probabilistic levels of toxic metals and health risk in cultivated and marketed vegetables in urban and peri-urban areas of Delhi, India.

A total of six vegetables (S. tuberosum, D. carota, S. lycopersicum, A. esculentus, S. oleracea and B. juncea) were analysed for five heavy metals (As, Cd, Cr, Hg, and Pb) to evaluate the contamination load in vegetables collected from five cultivated and two market sites (n = 504) at Delhi, India. The irrigation water samples and soil samples (n = 180) were only collected from cultivated sites. The results showed that the concentration of heavy metals in soil and water samples were well below the permissible level except for Cd 0.001-0.013 µg g-1. Similarly, the concentration of Cd (>0.20 µg g-1) was detected higher in all investigated vegetables except for tomato. The evaluation index value was highest for spinach and lowest for tomato. The transfer factor values and metal pollution index was maximum in spinach and okra. Principal component analysis (PCA), Tukey's HSD (Honestly Significant Difference) test, and one-way and two-way ANOVA (Analysis of Variance) were also applied to statistically analyse the results.

Wheat Cultivar Growth, Biochemical, Physiological and Yield Attributes Response to Combined Exposure to Tropospheric Ozone, Particulate Matter Deposition and Ascorbic Acid Application.

In the present study wheat (Triticum aestivum) cultivar HD 2967 was exposed to ambient and elevated levels of O3 and PM deposition, with and without exogenous application of ascorbic acid (AA). Cultivar HD 2967 exposed to eight treatments in free air O3 enrichment facility and the assessed results showed that wheat cultivar, growth, biochemical, physiological and yield attributes were variably but adversely affected by combined exposure to O3 and PM deposition. PM deposition clogged stomata and enhanced leaf temperature. However, plants exposed to O3 and PM deposition and treated with AA exhibited less reduction in yield as compared to plants exposed to O3 and PM deposition without AA treatment. The decline in grain yield of HD 2967 due to combined exposure of O3 and PM deposition were in the range of 4%-17%. AA spray partially mitigated ozone and PM deposition adverse impact and enhanced wheat yield by 16%.

Mitigation of yield-scaled greenhouse gas emissions from irrigated rice through Azolla, Blue-green algae, and plant growth-promoting bacteria.

Irrigated transplanted flooded rice is a major source of methane (CH4) emission. We carried out experiments for 2 years in irrigated flooded rice to study if interventions like methane-utilizing bacteria, Blue-green algae (BGA), and Azolla could mitigate the emission of CH4 and nitrous oxide (N2O) and lower the yield-scaled global warming potential (GWP). The experiment included nine treatments: T1 (120 kg N ha-1 urea), T2 (90 kg N ha-1 urea + 30 kg N ha-1 fresh Azolla), T3 (90 kg N ha-1 urea + 30 kg N ha-1 Blue-green algae (BGA), T4 (60 kg N ha-1 urea + 30 kg N ha-1 BGA + 30 kg N ha-1 Azolla, T5 (120 kg N ha-1 urea + Hyphomicrobium facile MaAL69), T6 (120 kg N ha-1 by urea + Burkholderia vietnamiensis AAAr40), T7 (120 kg N ha-1 by urea + Methylobacteruim oryzae MNL7), T8 (120 kg N ha-1 urea + combination of Burkholderia AAAr40, Hyphomicrobium facile MaAL69, Methylobacteruim oryzae MNL7), and T9 (no N fertilizer). Maximum decrease in cumulative CH4 emission was observed with the application of Methylobacteruim oryzae MNL7 in T7 (19.9%), followed by Azolla + BGA in T4 (13.2%) as compared to T1 control. N2O emissions were not significantly affected by the application of CH4-oxidizing bacteria. However, significantly lower (P<0.01) cumulative N2O emissions was observed in T4 (40.7%) among the fertilized treatments. Highest yields were observed in Azolla treatment T2 with 25% less urea N application. The reduction in yield-scaled GWP was at par in T4 (Azolla and BGA) and T7 (Methylobacteruim oryzae MNL7) treatments and reduced by 27.4% and 15.2% in T4 and T7, respectively, as compared to the T1 (control). K-means clustering analysis showed that the application of Methylobacteruim oryzae MNL7, Azolla, and Azolla + BGA can be an effective mitigation option to reduce the global warming potential while increasing the yield.

Interactive effect of elevated tropospheric ozone and carbon dioxide on radiation utilisation, growth and yield of chickpea (Cicer arietinum L.).

An experiment was conducted in the Free Air Ozone and Carbon dioxide Enrichment (FAOCE) facility to study the impact of elevated O3, CO2 and their interaction on chickpea crop (cv. Pusa-5023) in terms of phenology, biophysical parameters, yield components, radiation interception and use efficiency. The crop was exposed to elevated O3 (EO:60ppb), CO2 (EC:550 ppm) and their combined interactive treatment (ECO: EC+EO) during the entire growing season. Results revealed that the crop's total growth period was shortened by 10, 14 and 17 days under elevated CO2, elevated O3 and the combined treatment, respectively. Compared to ambient condition, the leaf area index (LAI) under elevated CO2 was higher by 4 to 28%, whilst it is reduced by 7.3 to 23.8% under elevated O3. The yield based radiation use efficiency (RUEy) was highest under elevated CO2 (0.48 g MJ-1), followed by combined (0.41 g MJ-1), ambient (0.38 g MJ-1) and elevated O3 (0.32 g MJ-1) treatments. Elevated O3 decreased RUEy by 15.78% over ambient, and the interaction results in a 7.8% higher RUEy. The yield was 31.7% more under elevated CO2 and 21.9% lower in elevated O3 treatment as compared to the ambient. The combined interactive treatment recorded a higher yield as compared to ambient by 9.7%. Harvest index (HI) was lowest under elevated O3 (36.10%), followed by ambient (39.18%), combined (40.81%), and highest was under elevated CO2 (44.18%). Chickpea showed a positive response to elevated CO2 resulting a 5% increase in HI as compared to ambient condition. Our findings quantified the positive and negative impacts of elevated O3, CO2 and their interaction on chickpea and revealed that the negative impacts of elevated O3 can be compensated by elevated CO2 in chickpea. This work promotes the understanding of crop behaviour under elevated O3, CO2 and their interaction, which can be used as valuable inputs for radiation-based crop simulation models to simulate climate change impact on chickpea crop.

60 years of fertilization and liming impacts on soil organic carbon stabilization in a sub-tropical Alfisol.

Limited information is available on the C stabilization mechanism of tropical soils under different management practices including long-term organic manuring, mineral fertilization alone, or in combination with lime. Hence, to understand the effect of continuous application (for 60 years) of organic manure, fertilizer, and lime alone or in combination on an acidic Alfisol, stabilization of soil organic carbon (SOC) was evaluated under maize (Zea mays L.) wheat (Triticum aestivum L.) cropping. There were eight treatments that included farmyard manure (FYM) and nitrogen (N) applied in terms of FYM, additional dose of phosphorus (P) and potassium (K) applied in terms of inorganic fertilizer (FYM + P'K'), FYM + P'K' with liming (FYM + P'K' + L) and NPK alone. These treatments were laid in a randomized block design with three replications. Results indicated that FYM + P'K' plots had maximum amount of SOC inside large macroaggregates. The value was 33 and 92% greater than only minerally fertilized (NPK) and unfertilized control plots, respectively, whereas microaggregate-associated C was highest in plots with FYM + P'K' and lime (FYM + P'K' + L), which was 48 and 183% more than unfertilized control and NPK plots, respectively. Inside soil microaggregates, plots under FYM + P'K' had highest labile C, while NPK + L plots had highest recalcitrant C. Plots with organic amendments contained higher glomalin in large macroaggregates. Plots treated with FYM + P'K' had maximum intra-aggregate particulate organic matter within microaggregates inside macroaggregates (iPOM_mM), which was 28 and 74% higher than NPK and unfertilized control plots, respectively. Total C stock inside the protected microaggregates within macroaggregates was maximum for FYM + P'K' plots. It had 38, 67, and 171% higher C stock than NPK, FYM, and unfertilized control plots, respectively. Interestingly, despite estimated C input in FYM-treated plots was much higher than NPK plots, FYM-treated plots had less C stabilization within microaggregates and within microaggregates inside macroaggregates. Microaggregates within macroaggregates accounted for ~54% of the recalcitrant C content. Thus, macroaggregates stabilization through occlusion of microaggregates was accountable for sequestration of SOC and only FYM application did not promote that mechanism compared to NPK. Carbon stabilization within macroaggregates under FYM plots was mainly governed by amorphous iron oxide.

Nickel in terrestrial biota: Comprehensive review on contamination, toxicity, tolerance and its remediation approaches.

Nickel (Ni) has been a subject of interest for environmental, physiological, biological scientists due to its dual effect (toxicity and essentiality) in terrestrial biota. In general, the safer limit of Ni is 1.5 µg g-1 in plants and 75-150 µg g-1 in soil. Litreature review indicates that Ni concentrations have been estimated up to 26 g kg-1 in terrestrial, and 0.2 mg L-1 in aquatic resources. In case of vegetables and fruits, mean Ni content has been reported in the range of 0.08-0.26 and 0.03-0.16 mg kg-1. Considering, Ni toxicity and its potential health hazards, there is an urgent need to find out the suitable remedial approaches. Plant vascular (>80%) and cortical (<20%) tissues are the major sequestration site (cation exchange) of absorbed Ni. Deciphering molecular mechanisms in transgenic plants have immense potential for enhancing Ni phytoremediation and microbial remediation efficiency. Further, it has been suggested that integrated bioremediation approaches have a potential futuristic path for Ni decontamination in natural resources. This systematic review provides insight on Ni effects on terrestrial biota including human and further explores its transportation, bioaccumulation through food chain contamination, human health hazards, and possible Ni remediation approaches.

Methane utilizing plant growth-promoting microbial diversity analysis of flooded paddy ecosystem of India.

Methane utilizing bacteria (MUB) are known to inhabit the flooded paddy ecosystem where they play an important role in regulating net methane (CH4) emission. We hypothesize that efficient MUB having plant growth-promoting (PGP) attributes can be used for developing novel bio-inoculant for flooded paddy ecosystem which might not only reduce methane emission but also assist in improving the plant growth parameters. Hence, soil and plant samples were collected from the phyllosphere, rhizosphere, and non-rhizosphere of five rice-growing regions of India at the tillering stage and investigated for efficient methane-oxidizing and PGP bacteria. Based on the monooxygenase activity and percent methane utilization on NMS medium with methane as the sole C source, 123 isolates were identified and grouped phylogenetically into 13 bacteria and 2 yeast genera. Among different regions, a significantly higher number of isolates were obtained from lowland flooded paddy ecosystems of Aduthurai (33.33%) followed by Ernakulum (20.33%) and Brahmaputra valley (19.51%) as compared to upland irrigated regions of Gaya (17.07%) and Varanasi (8.94%). Among sub-samples, a significantly higher number of isolates were found inhabiting the phyllosphere (58.54%) followed by non-rhizosphere (25.20%) and rhizosphere (15.45%). Significantly higher utilization of methane and PGP attributes were observed in 30 isolates belonging to genera Hyphomicrobium, Burkholderia, Methylobacterium, Paenibacillus, Pseudomonas, Rahnella, and Meyerozyma. M. oryzae MNL7 showed significantly better growth with 74.33% of CH4 utilization at the rate of 302.9 ± 5.58 and exhibited half-maximal growth rate, Ks of 1.92 ± 0.092 mg CH4 L-1. Besides the ability to utilize CH4, P. polymyxa MaAL70 possessed PGP attributes such as solubilization of P, K, and Zn, fixation of atmospheric N and production of indole acetic acid (IAA). Both these promising isolates can be explored in the future for developing novel biofertilizers for flooded paddies.

Inoculation of plant growth promoting-methane utilizing bacteria in different N-fertilizer regime influences methane emission and crop growth of flooded paddy.

Methane (CH4) emission in rice fields is greatly influenced by the type and quantity of nitrogenous fertilizer used. The net methane emission from paddy fields is also influenced by the activity of methane utilizing bacteria, which inhabit the flooded paddy ecosystem. Efficient methane utilizing and plant growth promoting bacteria Methylobacterium oryzae MNL7 and Paenibacillus polymyxa MaAL70, respectively were co-inoculated along with different nitrogenous fertilizer combinations in flooded paddy to assess their impact on cumulative methane emission and crop growth promotion. Co-inoculation significantly influenced the plant growth parameters of paddy, resulting in an increase in grain yield by 14.04, 11.08, and 12.38% in treatments receiving Urea, Di-ammonium Phosphate (DAP) + Urea, or farm yard manure (FYM), over their respective un-inoculated plots. Significant improvement in the rice grain nutrient quality in term of crude protein, Fe and Zn content was observed as a result of bacterial co-inoculation in FYM fertilized plots as compared to Urea and DAP+ Urea fertilized plots. Significantly higher cumulative methane emission of 63.39 kg ha-1 was observed in uninoculated plots fertilized with FYM treatment as compared to Urea (33.83 kg ha-1) and DAP+Urea (31.66 kg ha-1) treatments. Bacterial co-inoculation significantly reduced the cumulative methane emission by 12.03, 11.47 and 6.92% in Urea, DAP+Urea, and FYM fertilized plots over their respective uninoculated treatments. Among the different fertilizer treatments, bacterial co-inoculation with urea application performed significantly better in reducing cumulative methane emission. These findings suggest that methane utilizing bacteria which also possess plant growth promoting trait can be explored for developing a novel biofertilizer for flooded paddies, as they can aid in managing both the overall methane emission and enhancing crop yield.

Effect of elevated ozone and carbon dioxide interaction on growth, yield, nutrient content and wilt disease severity in chickpea grown in Northern India.

Wilt caused by Fusarium oxysporum, sp. Ciceris (FOC) is an important disease causing losses up to 10% in chickpea yield. Experiments were conducted growing chickpea in free air ozone and carbon dioxide enrichment rings under four treatments of elevated ozone (O3) (EO:60 ± 10 ppb), elevated carbon dioxide (CO2) (ECO2:550 ± 25 ppm), combination of elevated CO2 and O3 (EO + ECO2) and ambient control for quantifying the effect on growth, yield, biochemical and nutrient content of chickpea. For studying the impact on wilt disease, chickpea was grown additionally in pots with soil containing FOC in these rings. The incidence of Fusarium wilt reduced significantly (p < 0.01) under EO as compared to ambient and ECO2. The activities of pathogenesis-related proteins chitinase and ß-1,3- glucanase, involved in plant defense mechanism were enhanced under EO. The aboveground biomass and pod weight declined by 18.7 and 15.8% respectively in uninnoculated soils under EO, whereas, in FOC inoculated soil (diseased plants), the decline under EO was much less at 8.6 and 9.9% as compared to the ambient. Under EO, the activity of super oxide dismutase increased significantly (p < 0.5, 40%) as compared to catalase (12.5%) and peroxidase (17.5%) without any significant increase under EO + ECO2. The proline accumulation was significantly (p < 0.01) higher in EO as compared to EO + ECO2, and ECO2. The seed yield declined under EO due to significant reduction (p < 0.01) in the number of unproductive pods and seed weight. No change in the protein, total soluble sugars, calcium and phosphorus content was observed in any of the treatments, however, a significant decrease in potassium (K) content was observed under EO + ECO2. Elevated CO2 (554ppm) countered the impacts of 21.1 and 14.4 ppm h (AOT 40) O3 exposure on the seed yield and nutrient content (except K) in the EO + CO2 treatment and reduced the severity of wilt disease in the two years' study.

Raffinose and Hexose Sugar Content During Germination Are Related to Infrared Thermal Fingerprints of Primed Onion (Allium cepa L.) Seeds.

Priming is used to increase vigor, germination synchronization, seedling growth, and field establishment by advancing metabolic processes within seeds. Seed respiration is a good indicator of the metabolic processes that lead to transition toward germination. Onion seeds (cv. Pusa Ridhi) subjected to osmopriming (-1.5 MPa PEG6000 for 7 days), magnetopriming (100 mT for 30 min) and halopriming (150 mM KNO3 for 6 days), were evaluated at different times of imbibition to study the emergence index and respiration indices such as infrared thermal fingerprint, CO2 evolution rate, cytochrome c oxidase activity, and soluble sugars profile. Haloprimed seeds exhibited 42.5% higher emergence index as compared to unprimed control. Primed and unprimed seeds showed negative values for relative temperature (ΔT) (difference in temperature of seed and its immediate environment). Haloprimed seeds had the lowest values (-4.1 to -2.3°C) compared to other priming treatments over the germination period. Soluble sugars like raffinose, sucrose, glucose, and fructose contents were monitored and it was observed that en masse raffinose, glucose, and fructose levels were (17.5-59.9%) lower in haloprimed seeds over control. A positive correlation (r 2 = 0.504∗∗) was derived between the amount of these sugars and ΔT. Seed respiration, measured as CO2 evolution rate was more for haloprimed seeds that indicated that these soluble sugars were used as respiratory substrates. Significantly higher cytochrome c oxidase activity (40.7-89.8% and 12.5-66.6%) was observed in all primed seeds at 28 and 36 h, respectively. Among the various seed priming methods, halopriming proved to be the most effective priming treatment in onion seeds as evidenced by the higher respiration indices that resulted in faster metabolic rate and emergence index.

The effects of elevated CO2 and elevated O3 exposure on plant growth, yield and quality of grains of two wheat cultivars grown in north India.

Global food security is challenged by increasing levels of CO2, O3 and temperature trough their impacts on production and grain quality of wheat, one of the major C3 crops and staple food across the world. The present study was conducted to assess the effects of elevated levels of CO2 (EC; 550 ppm) and tropospheric O3 (EO; 70 ppb) as well as of combined interactive treatment [EC X EO; ECO] on plant growth, yield and grain quality of two wheat cultivars (HD-2967 and C-306) grown during 2016-17 and 2017-18 using free air ozone and carbon dioxide enrichment (FAOCE) facility under field conditions. Individually, EC, increased leaf area index (LAI; 15.9-28.2%), photosynthetic rate (Pn; 11.4-20.3%) and yield (8.2-20.9%) whereas EO declined LAI (5.1-12.5%), Pn (2.8-11.8%) and yield (2.2-14.2%) over ambient conditions (Amb: 405.2 ppm CO2 and 30.7 ppb O3). Under ECO condition, EC increased LAI (2.2-17.1%), Pn (2.8-17.6%) and grain yield parameters (4.4-24.3%) across the cultivars in both years, but reduced the positive effects of EO on quality as compared to Amb. Dilution effect of increased yield under EC condition have reduced total protein, micro- and macro-nutrient concentrations whereas EO increased them notably compared to Amb. Starch in grains increased under EC but reduced under EO as compared to Amb. AOT40, the sum of averaged difference of O3 h-1 concentration beyond 40 ppb for 7 hours (31233 ppb h-1) in FAOCEs rings during the crop growth period led to reduction in average grain yield of HD-2967 and C-306 by 11.6 and 8.5% or by 1.6 and 1.3% yield loss per ppb increase of O3, respectively. The growth, yield and quality parameters of both wheat cultivars responded similarly but to different extent to all treatments. EC was able to offset the negative effects of EO on yield and yield components only, but not those concerning the quality of grains. To stabilize global food security, precursor gases forming tropospheric ozone must be constrained.

Effect of elevated carbon dioxide on soil hydrothermal regimes and growth of maize crop (Zea mays L.) in semi-arid tropics of Indo-Gangetic Plains.

To see the effect of climate change on the variation of soil hydrothermal regimes and growth of maize crop, an experiment was conducted in free-air carbon dioxide enrichment (FACE) facility during the kharif season of 2015 at Climate Change Facility of Indian Agricultural Research Institute, New Delhi, India. Under elevated CO2 and ambient condition, surface bulk density (BD) were 1.38 Mgm-3 and 1.44 Mgm-3, respectively but BD were not significantly different. During different days after sowing (DAS), in 0 to 10-cm soil depth, soil water content (SWC) in FACE varied between 14.58-20.70%, whereas in ambient condition, SWC variations were in between 19.33-22.94%. In 10 to 20-cm soil depth, SWC ranged in between 20.47-27.14% in FACE and 23.57-25.42% in ambient condition for different DAS. It is also observed that the arrival of peak surface ST was 1 h early in elevated CO2 condition. Photosynthetic rate increased by 5.7% on 44 DAS and 18.1% on 70 DAS under elevated carbon dioxide condition. Elevated carbon dioxide had reduced the stomatal conductance but the reduction was not significant. Like variation in air temperature for climate change, more intensive study is required to see the effect of climate change on soil temperature and its effect on crop growth.