Nitrogen reductions have decreased hypoxia in the Chesapeake Bay: Evidence from empirical and numerical modeling.

Seasonal hypoxia is a characteristic feature of the Chesapeake Bay due to anthropogenic nutrient input from agriculture and urbanization throughout the watershed. Although coordinated management efforts since 1985 have reduced nutrient inputs to the Bay, oxygen concentrations at depth in the summer still frequently fail to meet water quality standards that have been set to protect critical estuarine living resources. To quantify the impact of watershed nitrogen reductions on Bay hypoxia during a recent period including both average discharge and extremely wet years (2016-2019), this study employed both statistical and three-dimensional (3-D) numerical modeling analyses. Numerical model results suggest that if the nitrogen reductions since 1985 had not occurred, annual hypoxic volumes (O2 < 3 mg L-1) would have been ~50-120% greater during the average discharge years of 2016-2017 and ~20-50% greater during the wet years of 2018-2019. The effect was even greater for O2 < 1 mg L-1, where annual volumes would have been ~80-280% greater in 2016-2017 and ~30-100% greater in 2018-2019. These results were supported by statistical analysis of empirical data, though the magnitude of improvement due to nitrogen reductions was greater in the numerical modeling results than in the statistical analysis. This discrepancy is largely accounted for by warming in the Bay that has exacerbated hypoxia and offset roughly 6-34% of the improvement from nitrogen reductions. Although these results may reassure policymakers and stakeholders that their efforts to reduce hypoxia have improved ecosystem health in the Bay, they also indicate that greater reductions are needed to counteract the ever-increasing impacts of climate change.

Major point and nonpoint sources of nutrient pollution to surface water have declined throughout the Chesapeake Bay watershed.

Understanding drivers of water quality in local watersheds is the first step for implementing targeted restoration practices. Nutrient inventories can inform water quality management decisions by identifying shifts in nitrogen (N) and phosphorus (P) balances over space and time while also keeping track of the likely urban and agricultural point and nonpoint sources of pollution. The Chesapeake Bay Program's Chesapeake Assessment Scenario Tool (CAST) provides N and P balance data for counties throughout the Chesapeake Bay watershed, and these data were leveraged to create a detailed nutrient inventory for all the counties in the watershed from 1985-2019. This study focuses on three primary watershed nutrient balance components-agricultural surplus, atmospheric deposition, and point source loads-which are thought to be the leading anthropogenic drivers of nutrient loading trends across the watershed. All inputs, outputs, and derived metrics (n=53) like agricultural surplus and nutrient use efficiency, were subjected to short- and long-term trend analyses to discern how sources of pollution to surface water have changed over time. Across the watershed from 1985-2019, downward trends in atmospheric deposition were ubiquitous. Though there are varying effects, long-term declines in agricultural surplus were observed, likely because nutrients are being managed more efficiently. Multiple counties' point source loads declined, primarily associated with upgrades at major cities that discharge treated wastewater directly to tidal waters. Despite all of these positive developments, recent increases in agricultural surpluses from 2009-2019 highlight that water quality gains may soon be reversed in many agricultural areas of the basin. Besides tracking progress and jurisdictional influence on pollution sources, the nutrient inventory can be used for retrospective water quality analysis to highlight drivers of past improvement/degradation of water quality trends and for decision makers to develop and track their near- and long-term watershed restoration strategies.

Atmospheric nitrogen deposition in the Chesapeake Bay watershed: A history of change.

The Chesapeake Bay watershed has been the focus of pioneering studies of the role of atmospheric nitrogen (N) deposition as a nutrient source and driver of estuarine trophic status. Here, we review the history and evolution of scientific investigations of the role of atmospheric N deposition, examine trends from wet and dry deposition networks, and present century-long (1950-2050) atmospheric N deposition estimates. Early investigations demonstrated the importance of atmospheric deposition as an N source to the Bay, providing 25%-40% among all major N sources. These early studies led to the unprecedented inclusion of targeted decreases in atmospheric N deposition as part of the multi-stakeholder effort to reduce N loads to the Bay. Emissions of nitrogen oxides (NOx) and deposition of wet nitrate, oxidized dry N, and dry ammonium ( NH 4 + ) sharply and synchronously declined by 60%-73% during 1995-2019. These decreases largely resulted from implementation of Title IV of the 1990 Clean Air Act Amendments, which began in 1995. Wet NH 4 + deposition shows no significant trend during this period. The century-long atmospheric N deposition estimates indicate an increase in total atmospheric N deposition in the Chesapeake watershed from 1950 to a peak of ~15 kg N/ha/yr in 1979, trailed by a slight decline of <10% through the mid-1990s, and followed by a sharp decline of about 40% thereafter through 2019. An additional 21% decline in atmospheric N deposition is projected from 2015 to 2050. A comparison of the Potomac River and James River watersheds indicates higher atmospheric N deposition in the Potomac, likely resulting from greater emissions from higher proportions of agricultural and urban land in this basin. Atmospheric N deposition rose from 30% among all N sources to the Chesapeake Bay watershed in 1950 to a peak of 40% in 1973, and a decline to 28% by 2015. These data highlight the important role of atmospheric N deposition in the Chesapeake Bay watershed and present a potential opportunity for decreases in deposition to contribute to further reducing N loads and improving the trophic status of tidal waters.

Revised Method and Outcomes for Estimating Soil Phosphorus Losses from Agricultural Land in the Chesapeake Bay Watershed Model.

Current restoration efforts for the Chesapeake Bay watershed mandate a timeline for reducing the load of nutrients and sediment into receiving waters. The Chesapeake Bay watershed model (WSM) has been used for two decades to simulate hydrology and nutrient and sediment transport; however, spatial limitations of the WSM preclude edge-of-field scale representation of phosphorus (P) losses. Rather, the WSM relies on literature-derived, county-scale rates of P loss (targets) for simulated land uses. An independent field-scale modeling tool, Annual Phosphorus Loss Estimator (APLE), was used as an alternative to the current WSM approach. Identical assumptions of county-level acreage, soil properties, nutrient management practices, and transport factors from the WSM were used as inputs to APLE. Incorporation of APLE P-loss estimates resulted in greater estimated total P loss and a revised spatial pattern of P loss compared with the WSM's original targets. Subsequently, APLE's revised estimates for P loss were substituted into the WSM and resulted in improved WSM calibration performance at up to 79% of tributary monitoring stations. The incorporation of APLE into the WSM will improve its ability to assess P loss and the impact of field management on Chesapeake Bay water quality.

Dark secrets behind the shimmer of contact lens: the Indian scenario.

BACKGROUND: We studied the bacteriological profile of soft contact lens and its accessories among the asymptomatic subjects and monitored the compliance level with the lens use and its cleaning protocol. FINDINGS: A total of 115 (104 daily wear and 11 extended wear) subjects using contact lens were studied. Data regarding the duration of use and frequency and method of cleaning were recorded. Contact lens, lens cases, preserving solutions and tips of solution bottles were the samples collected. The isolates were identified on the basis of their phenotypic characters. Samples from 24 subjects (21 daily wear and 3 extended wear) were found contaminated. Of the 24 contaminated cases, 23 showed medium adherence to the cleaning protocol. Contamination rate was higher among the 56 daily wear lens users who used same lens for 2 years and more, than the 48 users who used their lenses for less than 2 years. Lens case contamination was found in all the 24 cases. The bacteria isolated were Citrobacter freundii, Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, Staphylococcus epidermidis and Streptococcus pneumoniae. In extended wear lens users, there was no change in microbial flora on repeating the cultures on day 7 and 14. CONCLUSION: Non-compliance with contact lens use may lead to invitation of microbial flora. The accumulation of these bacteria may act as a precursor to biofilm formation, thus colonizing the lens accessories as well. The bacteria isolated in this study were similar to the ones causing microbial keratitis thus, predisposing the otherwise asymptomatic subjects to permanent visual damage.