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Exposure to particulate matter less than 2.5 µm in diameter (PM2.5) is a cause of concern in cities and major emission regions of northern India. An intensive field campaign involving the states of Punjab, Haryana and Delhi national capital region (NCR) was conducted in 2022 using 29 Compact and Useful PM2.5 Instrument with Gas sensors (CUPI-Gs). Continuous observations show that the PM2.5 in the region increased gradually from < 60 µg m-3 in 6-10 October to up to 500 µg m-3 on 5-9 November, which subsequently decreased to about 100 µg m-3 in 20-30 November. Two distinct plumes of PM2.5 over 500 µg m-3 are tracked from crop residue burning in Punjab to Delhi NCR on 2-3 November and 10-11 November with delays of 1 and 3 days, respectively. Experimental campaign demonstrates the advantages of source region observations to link agricultural waste burning and air pollution at local to regional scales.
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The present study provides details about the usefulness of chlorination in the recovery effluents of sewage, and to make it useable for irrigation purposes. Chlorination is one of the effective simplified, and cost-effective traditional methods for disinfection. The study was done for the period of March, 2019 to February, 2020. The disinfection process was optimized by adding sodium hypochlorite to the secondary treated effluents with the help of jar apparatus at a mixing speed of 100 rpm. To optimize the various process variables such as dose, and contact time, several concentrations of NaOCl (0.5, 1, 1.5, 2, 2.5, 3.0) ppm were carefully chosen at different time intervals of 15, 30, and 60 min respectively, which were centered on the foregoing studies. The factors like seasonal variation on MPN index of total coliforms (TCs), CR ∗ T concept, and effect of pH on log elimination of TCs, outcome of pH with rate constant (k), and results of pH against dilution coefficient (n) was also studied. The Chick-Watson, Rennecker-Marinas, Collin-Selleck, and modified Selleck models have shown good reliability to the experimental data of chlorine disinfection to be fit into these kinetic models for the treatment of sewage wastewater. The upgraded CR ∗ T values were attained by using disinfection models. Among these four models, the kinetic modeling by Collin-Selleck, and Selleck-White was investigated as the best modeling to be fitted more finely to the chlorination experimental data to count for the effectiveness of NaOCl. The selected indicator organism in the optimization process of chlorine was Total coliforms (TCs). The residual chlorine and most probable number per log unit (Log) for TCs were measured before the start and after the termination of the disinfection process. The World Health Organization (WHO) standard for pathogenic removal from wastewater, and to irrigate the crops is 3- to 4-log and the chlorine residual under 1 mg/l limit was accomplished.
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Severe fog events during winter months in India are a serious concern due to the higher incidence of road accidents, flight delays and increased occurrence of respiratory diseases. The present paper is an attempt to study the twenty fog samples collected from the rooftop of an academic building of Guru Nanak Dev University, Amritsar, India from November 2017 to January 2018. Fog samples were analysed for various parameters viz. pH, electrical conductivity (EC), chloride (Cl-), nitrate (NO3 -) and sulphate (SO4 2-) levels. The pH, EC, and Cl-, NO3 - and SO4 2- levels in the fog samples were estimated as 6.3-7.9, 240-790 µS cm-1, 108-2025 µeq L-1, 105-836 µeq L-1 and 822-5642 µeq L-1, respectively. It was noticed that sulphate was the dominant anion in fog samples. The SO4 2- to NO3 - molar ratio in the fog was estimated as 7.6 which suggests the burning of fossil fuel as the major pollutant from vehicular exhausts. Multiple regression analysis was performed to evaluate the effect of PM2.5/PM10 ratio and relative humidity (RH) on visibility. A box-cox plot of power transformation produced better model fitting, employing a square root transformation of the visibility which indicated that the PM2.5/PM10 and RH have an exponential effect on visibility.
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The current study uses the free radical graft copolymerization of acrylic acid as a monomer, N,N-methylene-bis-(acrylamide) as a crosslinker and ammonium persulfate as an initiator to synthesise GG-cl-poly(AA) hydrogels based on gellan gum utilising response surface methodology (RSM). A full factorial design was used to obtain the greatest percent swelling (P s), and key process variables were determined using the Pareto chart. To make the procedure cost-effective, a multiple regression model employing ANOVA projected a linear model with a maximum percentage swelling of 556 at the lowest concentration of all three studied factors. As a result, the sequential experimental design was successful in obtaining two-fold increases in the percentage swelling in a systematic way. An RSM-based central composite design was used to optimize the percentage swelling of the three most important synthesis parameters: initiator concentration, monomer concentration, and crosslinker concentration. The best process conditions are 7.3 mM L-1 initiator, 44 µM L-1 monomer, and 21.6 mM L-1 crosslinker. The effective synthesis of GG-cl-poly(AA) was validated by Fourier transform infrared spectroscopy (FTIR), X-ray diffraction, field emission scanning electron microscopy (FE-SEM), and 1H-nuclear magnetic resonance. The swelling behavior of GG-cl-poly(AA) in water and saline solutions, as well as its water retention capability, was investigated. In comparison to distilled water, the swelling potential of optimized hydrogel was shown to be significantly reduced in saline solutions. The addition of GG-cl-poly(AA) significantly improved the moisture properties of plant growth media (clay, sandy, and clay-soil combination), implying that it has great potential in moisture stress agriculture. GG-cl-poly(AA) biodegradation was studied by soil burial and vermicomposting methods. The composting approach showed 89.95% deterioration after 22 days, while the soil burial method showed 86.71% degradation after 22 days. The synthesized hydrogel may be beneficial for agricultural applications because of its considerable degradation behaviour, strong water retention capacity, low cost, and environmental friendliness.
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Fibromyalgia (FM) is a chronic pain syndrome involving complex interplay of biogenic amines and NMDA receptor mediated hypersensitization of nociceptive pathways. Clinical management of FM is poorly addressed with only a few available therapeutic options. Coumarins are active phenolic molecules of natural origin found to have broad pharmacological activities. Current investigation explores the role of naturally occurring coumarin, imperatorin in mouse model of fibromyalgia. Administration of reserpine (0.5â¯mg/kg, s.c.) thrice at 24â¯h intervals induced behavioral and neurochemical alterations characteristic of fibromyalgia. Reserpine was found to induce allodynia quantified using electronic von Frey (e-VF) and pressure application measurement (PAM) test, depression as indicated by an increased duration of immobility in forced swim test (FST), decreased motor coordination and locomotor activity in inclined plane test (IPT) and open field test (OFT) respectively. Cognitive deficits were evident by an increased latency to locate hidden platform in Morris water maze (MWM) and passive avoidance test (PAT). Reserpine treatment was found to cause an increased anxiety as revealed by increased time spent in closed arm of the elevated plus maze (EPM). Furthermore, an up- regulation in NMDA and NFκB expression in the brain and spinal cord was observed in reserpine treated groups. Administration of imperatorin (10â¯mg/kg, i.p) for a period of 5â¯days ameliorated all behavioral deficits, biochemical changes and decreased expression of NMDA and NFκB in the brain and spinal cord of treated mice. These findings indicate an interplay of NMDA/NFκB modulation by imperatorin in the reserpine induced fibromyalgia in mice.
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Fibromialgia/tratamiento farmacológico , Fibromialgia/metabolismo , Furocumarinas/uso terapéutico , N-Metilaspartato/metabolismo , FN-kappa B/metabolismo , Reserpina/toxicidad , Inhibidores de Captación Adrenérgica/toxicidad , Animales , Relación Dosis-Respuesta a Droga , Fibromialgia/inducido químicamente , Furocumarinas/farmacología , Ratones , N-Metilaspartato/agonistas , N-Metilaspartato/antagonistas & inhibidores , FN-kappa B/agonistas , FN-kappa B/antagonistas & inhibidores , Transducción de Señal/efectos de los fármacos , Transducción de Señal/fisiología , Resultado del TratamientoRESUMEN
In continuation with our previous studies on osthole, bergapten, a closely related furanocoumarin was investigated for its ameliorative effect on chemically induced neurogenic and inflammatory hyperalgesia and inflammation in mice. Chemical hyperalgesia and inflammation was induced by administration of formalin (intraplantar), acetic acid (intraperitoneal) and carrageenan (intraplantar) to different groups of animals. Pain responses were quantified and median effective dose (ED50) of bergapten was calculated. Lipopolysaccharide challenge was administered to study inflammatory cytokines which were analyzed in plasma using ELISA. The expression of poly ADP-ribose polymerase (PARP), cyclooxygenase (COX-2) and inducible nitric oxide synthase (iNOS) was quantified by immnofluorescence staining. Bergapten was found to ameliorate both neurogenic and inflammatory hyperalgesia precipitated by formalin, acetic acid induced writhing and carrageenan induced paw inflammation with ED50 dose of 2.96 mg/kg. Bergapten also significantly decreased the levels of TNF-α and IL-6 and the expression of PARP, COX-2 and iNOS in the spine. It is concluded that bergapten is an interesting molecule with significant analgesic and anti-inflammatory activity emanating through the modulation of multiple pain mediating pathways.
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5-Metoxipsoraleno/farmacología , Ciclooxigenasa 2/metabolismo , Citocinas/metabolismo , Inflamación/tratamiento farmacológico , Óxido Nítrico Sintasa de Tipo II/metabolismo , Dolor Nociceptivo/tratamiento farmacológico , Inhibidores de Poli(ADP-Ribosa) Polimerasas/metabolismo , Columna Vertebral/efectos de los fármacos , Analgésicos/farmacología , Animales , Antiinflamatorios/farmacología , Carragenina/farmacología , Femenino , Hiperalgesia/tratamiento farmacológico , Hiperalgesia/metabolismo , Inflamación/metabolismo , Mediadores de Inflamación/metabolismo , Lipopolisacáridos/farmacología , Masculino , Ratones , Dolor Nociceptivo/metabolismo , Columna Vertebral/metabolismoRESUMEN
Fibromyalgia is a chronic complex syndrome of non-articulate origin characterized by musculoskeletal pain, painful tender points, sleep problems and co-morbidities including depression, migraine. The etiopathogenesis of fibromyalgia is complex, variable and remains inconclusive. The etiological factors that have been defined include stress, genetic predisposition and environmental components. As per the reports of the American College of Rheumatology (ACR) the prevalence of fibromyalgia varies from 2 to 22% among the general population with poor diagnostic features primarily pain. Fibromyalgia encompasses a spectrum of co-morbid conditions with multifarious pathogenesis. The highly prevalent manifestations of fibromyalgia include heterogeneous pain and aches. Biochemical and neurobiological elements of fibromyalgia include neurotransmitters, hypothalamic pituitary adrenal axis (HPA axis), inflammatory cytokines, monoaminergic pathway, opioid peptides, sex hormones, nerve growth factor (NGF) and local free radical insult. An imbalance in the serotonergic system is the major underlying etiological factor that has been explored most widely. Owing to complex interplay of diverse pathophysiological pathways, overlapping co-morbidities such as depression have been clinically observed. Therapeutic management of fibromyalgia involves both non pharmacological and pharmacological measures. The current review presents various dysregulations and their association with symptoms of fibromyalgia along with their underlying neurobiological aspects.
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Depresión/etiología , Fibromialgia/etiología , Hepatitis C Crónica/etiología , Enfermedades Inflamatorias del Intestino/etiología , Trastornos Migrañosos/etiología , Animales , Comorbilidad , Citocinas/metabolismo , Depresión/metabolismo , Depresión/fisiopatología , Fibromialgia/metabolismo , Fibromialgia/fisiopatología , Hepatitis C Crónica/metabolismo , Hepatitis C Crónica/fisiopatología , Humanos , Sistema Hipotálamo-Hipofisario/metabolismo , Sistema Hipotálamo-Hipofisario/fisiopatología , Enfermedades Inflamatorias del Intestino/metabolismo , Enfermedades Inflamatorias del Intestino/fisiopatología , Péptidos y Proteínas de Señalización Intercelular/metabolismo , Trastornos Migrañosos/metabolismo , Trastornos Migrañosos/fisiopatología , Neuropéptidos/metabolismo , Estrés Oxidativo/fisiología , Dolor/metabolismo , Dolor/fisiopatología , Sistema Hipófiso-Suprarrenal/metabolismo , Sistema Hipófiso-Suprarrenal/fisiopatologíaRESUMEN
This study reports thermophilic fungus Malbranchea flava as a potent source of xylanase and xylan-debranching accessory enzymes. M. flava produced high levels of xylanase on sorghum straw containing solidified culture medium. The optimization of culture conditions for production of hemicellulases was carried out using one factor at a time approach and Box-Behnken design of experiments with casein (%), inoculum age (h) and inoculum level (ml) as process variables and xylanase, ß-xylosidase, acetyl esterases and arabinofuranosidase as response variables. The results showed that casein concentration between 3.0 and 3.5 %, inoculum age (56-60 h) and inoculum level (2-2.5 ml) resulted in production of 16,978, 10.0, 67.7 and 3.8 (U/gds) of xylanase, ß-xylosidase, acetyl esterase and α-L-arabinofuranosidase, respectively. Under optimized conditions M. flava produced eight functionally diverse xylanases with distinct substrate specificity against different xylan types. The peptide mass fingerprinting of 2-D gel electrophoresis resolved proteins indicated to the presence of cellobiose dehydrogenase and glycosyl hydrolases suggesting the potential of this strain in oxidative and classical cellulase-mediated hydrolysis of lignocellulosics. Addition of xylanase (300 U/g substrate) during saccharification (at 15 % substrate loading) of different pretreated (acid/alkali) substrates (cotton stalks, wheat straw, rice straw, carrot grass) by commercial cellulase (NS28066) resulted in 9-36 % increase in saccharification and subsequent fermentation to ethanol when compared to experiment with commercial enzyme only. High ethanol level 46 (g/l) was achieved with acid pretreated cotton stalk when M. flava xylanase was supplemented as compared to 39 (g/l) with xylanase without xylanase addition.
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Electrochemical degradation of gentamicin was achieved using a laboratory scale electrochemical reactor by optimizing pH, current density and treatment time. A two step statistical optimization was performed as per factorial design and center composite design (CCD). A Pareto chart was used for selecting statistically significant effects and an analysis of variance (ANOVA) table indicated significant curvature. Thus adding additional experimental runs improved the model fitting through a second order model. Maximum degradation was predicted at a pH of 6.7, 70 A m(-2) and 45 min. The experimental data fitted well through a reduced quadratic model with R(2) equal to 0.945. The toxicity of degradation products as determined by disc diffusion assay employing Pseudomonas aeruginosa strain was found to be reduced by 55%. The degradation pathway of gentamicin was studied using mass spectral (MS) analysis. Pure gentamicin showed a molecular ion peak at m/z 478 ([M + 1](+)), and after addition of NaCl as electrolyte, the mass peak was observed at m/z 523. After 15 min of electrochemical treatment, a new peak appeared at m/z 316 due to the loss of one pyran moiety. After 45 min of electrochemical treatment, another peak appeared at m/z of 478 due to loss of two Na(+) from gentamicin.
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Antibacterianos/aislamiento & purificación , Técnicas Electroquímicas , Gentamicinas/aislamiento & purificación , Análisis de Varianza , Antibacterianos/metabolismo , Antibacterianos/toxicidad , Gentamicinas/metabolismo , Gentamicinas/toxicidad , Pseudomonas aeruginosa/metabolismo , Espectrometría de Masa por Ionización de ElectrosprayRESUMEN
The present study envisages the performance of a laboratory scale electrocoagulation system for the removal of Cr(VI) from 100 mg l(-1) solution using Al-Al electrodes with an effective surface area of 100 cm(2), and placed 15 mm apart. The interaction between voltage x time, and amperage x time best explained the Cr(VI) reduction efficiency with the coefficient of determination (R(2)) being 0.8873 and 0.9270 respectively. Similarly, the square root of energy consumption in Cr(VI) reduction had a linear correlation with voltage x time (R(2)=0.8949), whereas, amperage x time better explained energy consumption (R(2)=0.9400). Response surface methodology was used for the optimization of process variables (pH, voltage and treatment time), response modeling and predictions. Maximum Cr(VI) reduction efficiency of 90.4% was achieved at pH 5, 24 V and 24 min treatment time, and the treatment consumed 137.2 KWh m(-3) of electrical energy. Multiple response optimization for maximizing Cr(VI) reduction efficiency and minimizing energy consumption showed 49.6% Cr(VI) removal at pH 5, 12.8 V and 24 min treatment time. The response models developed explained 95.2% variability for Cr(VI) reduction efficiency and 99.4% variability for energy consumption. Results of the prediction models were validated through laboratory scale batch experiments.