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1.
Zhongguo Zhong Yao Za Zhi ; 48(18): 4843-4851, 2023 Sep.
Artículo en Chino | MEDLINE | ID: mdl-37802826

RESUMEN

To investigate the mechanism by which Cangxi Tongbi Capsules promote chondrocyte autophagy to inhibit knee osteoarthritis(KOA) progression by regulating the circRNA_0008365/miR-1271/p38 mitogen-activated protein kinase(MAPK) pathway. The cell and animal models of KOA were established and intervened with Cangxi Tongbi Capsules, si-circRNA_0008365, si-NC, and Cangxi Tongbi Capsules combined with si-circRNA_0008365. Flow cytometry and transmission electron microscopy were employed to determine the level of apoptosis and observe autophagosomes, respectively. Western blot was employed to reveal the changes in the protein levels of microtubule-associated protein light chain 3(LC3)Ⅱ/Ⅰ, Beclin-1, selective autophagy junction protein p62/sequestosome 1, collagen Ⅱ, a disintegrin and metalloproteinase with thrombospondin motifs 5(ADAMTS-5), and p38 MAPK. The mRNA levels of circRNA_0008365, miR-1271, collagen Ⅱ, and ADAMTS-5 were determined by qRT-PCR. Hematoxylin-eosin staining was employed to reveal the pathological changes of the cartilage tissue of the knee, and enzyme-linked immunosorbent assay to measure the levels of interleukin-1ß(IL-1ß) and tumor necrosis factor-alpha(TNF-α). The chondrocytes treated with IL-1ß showed down-regulated expression of circRNA_0008365, up-regulated expression of miR-1271 and p38 MAPK, lowered autophagy level, increased apoptosis rate, and accelerated catabolism of extracellular matrix. The intervention with Cangxi Tongbi Capsules up-regulated the expression of circRNA_0008365, down-regulated the expression of miR-1271 and p38 MAPK, increased the autophagy level, decreased the apoptosis rate, and weakened the catabolism of extracellular matrix. However, the effect of Cangxi Tongbi Capsules was suppressed after interfering with circRNA_0008365. The in vivo experiments showed that Cangxi Tongbi Capsules dose-dependently inhibited the p38 MAPK pathway, enhanced chondrocyte autophagy, and mitigated articular cartilage damage and inflammatory response, thereby inhibiting the progression of KOA in rats. This study indicated that Cangxi Tongbi Capsules promoted chondrocyte autophagy by regulating the circRNA_0008365/miR-1271/p38 MAPK pathway to inhibit the development of KOA.


Asunto(s)
MicroARNs , Osteoartritis de la Rodilla , Ratas , Animales , Condrocitos , Osteoartritis de la Rodilla/metabolismo , Osteoartritis de la Rodilla/patología , ARN Circular/genética , ARN Circular/metabolismo , ARN Circular/farmacología , Proteínas Quinasas p38 Activadas por Mitógenos/genética , Proteínas Quinasas p38 Activadas por Mitógenos/metabolismo , MicroARNs/genética , MicroARNs/metabolismo , Apoptosis , Autofagia/genética , Colágeno/metabolismo
2.
Phytomedicine ; 111: 154663, 2023 Mar.
Artículo en Inglés | MEDLINE | ID: mdl-36657317

RESUMEN

BACKGROUND: Achyranthes bidentata Blume (A. bidentata) is a common Chinese herb used to treat osteoarthritis (OA). Achyranthoside D (Ach-D) is a glucuronide saponin isolated from A. bidentata. PURPOSE: To assess the mechanisms of action of Ach-D and its effects on OA. METHODS: The effects of Ach-D were evaluated in rats underwent anterior cruciate ligament transection (ACLT) with medial meniscectomy (MMx) and in interleukin (IL)-1ß-induced chondrocytes. Histological changes in rat cartilage tissues were detected using Safranin O-Fast green and haematoxylin-eosin staining. Immunohistochemical staining, qRT-PCR, ELISA, immunoblotting, and immunofluorescence were conducted to examine cartilage degeneration-related and inflammation-related factor expression. CCK-8, LDH assay, and EdU staining were performed to detect chondrocyte death. RESULTS: Ach-D dose-dependently reduced the Osteoarthritis Research Society International (OARSI) scores, alleviated cartilage injury, and decreased the serum concentrations of CTX-II and COMP in ACLT-MMx models. Ach-D increased the expression levels of collagen II and aggrecan and decreased the levels of cartilage degeneration-related proteins, ADAMTS-5, MMP13, and MMP3, in rat cartilage tissues. Additionally, nod-like receptor protein 3 (NLRP3)-related inflammation was reduced by Ach-D, as shown by the significantly inhibited expression levels of NLRP3, ASC, GSDMD, IL-6, TNF-α, IL-1ß, and IL-18 in rat cartilage tissues. In primary rat chondrocytes, Ach-D protected against IL-1ß-induced viability loss and LDH release. Wnt3a is the target protein of Ach-D. Mechanistically, Ach-D alleviated OA by inhibiting Wnt signalling. CONCLUSION: ACH-D may reduce inflammation and cartilage degeneration by inhibiting the Wnt signalling pathway, thereby reducing OA.


Asunto(s)
Cartílago Articular , Osteoartritis , Saponinas , Animales , Ratas , Condrocitos , Modelos Animales de Enfermedad , Inflamación/metabolismo , Interleucina-1beta/metabolismo , Proteína con Dominio Pirina 3 de la Familia NLR/metabolismo , Osteoartritis/metabolismo , Saponinas/metabolismo , Proteína Wnt3/metabolismo
3.
Medicine (Baltimore) ; 101(39): e30847, 2022 Sep 30.
Artículo en Inglés | MEDLINE | ID: mdl-36181082

RESUMEN

A minimally invasive approach has been widely used for the treatment of calcaneal fractures, however, its downside in exposing the calcaneal body, affects fracture reduction. We used a Steinmann pin retractor mentioned in our previous case series study to solve this issue. To further evaluate the efficacy of this reduction technique, and elucidate its mechanism, we compared it with the control groups in this study. Between March 2017 and November 2020, 52 patients were included in this retrospective comparative study. 20 patients were included in the S-S group (Steinmann pin retractor with internal fixation via the sinus tarsi approach), 17 patients were included in the S group (internal fixation via sinus tarsi approach), and 15 patients were included in the L group (internal fixation via L-shaped approach). Patients in each group were operated on by a distinctly skilled surgeon who is specialized in one of the specified surgeries mentioned above. All patients received positive postoperative radiological and clinical evaluations. The patients were followed up for at least 6 months postoperatively. At the last S-S group follow-up, the VAS value (0.29 ±â€…0.46) improved compared to the L group. The AOFAS (American Orthopedic Foot and Ankle Society) Ankle-Hindfoot score (94.38 ±â€…5.05) also improved significantly compared to the S and L groups. The Böhler angle (32.34 ±â€…3.56°), width (36.48 ±â€…3.91 mm), and height (88.87 ±â€…4.12 mm) of the calcaneal improved (34.38 ±â€…18.50°, -10.13 ±â€…6.98 mm, 8.75 ±â€…4.82 mm) compared to the preoperative state, the S and L groups. These parameters (the Böhler angle: 31.76 ±â€…3.37°, width: 36.47 ±â€…3.72 mm, height: 87.23 ±â€…3.83 mm) were maintained at the last follow-up. Steinmann pin retractor effected a sound reduction. It also manifested radiological and clinical advantages over the S and L protocols. By reason of the aforementioned, it could be recommended as useful reduction equipment for the minimally invasive treatment of calcaneal fractures.


Asunto(s)
Traumatismos del Tobillo , Calcáneo , Traumatismos de los Pies , Fracturas Óseas , Fracturas Intraarticulares , Traumatismos de la Rodilla , Calcáneo/cirugía , Fijación Interna de Fracturas/métodos , Fracturas Óseas/cirugía , Humanos , Fracturas Intraarticulares/cirugía , Procedimientos Quirúrgicos Mínimamente Invasivos/métodos , Estudios Retrospectivos , Resultado del Tratamiento
4.
Ann Transl Med ; 10(1): 23, 2022 Jan.
Artículo en Inglés | MEDLINE | ID: mdl-35242868

RESUMEN

BACKGROUND: Knee osteoarthritis (KOA) is a leading cause of chronic pain and disability, and as such, it poses a significant economic burden. Traditional Chinese medicine (TCM), as well as complementary and alternative medicine, can offer safe and effective treatments for KOA. Cangxitongbi (CXTB) capsule is a Chinese patented medicine for KOA treatment and has a remarkable curative effect. This article evaluated the effects and mechanisms of CXTB in protecting joint cartilage in vivo. METHODS: The KOA model was constructed in rats using the modified Hulth method. CXTB (35 mg/kg) was administered intragastrically for 4 weeks. Hematoxylin and eosin (HE) staining of the knee articular were performed to evaluate the efficiency of CXTB. Western blot analysis, quantitative polymerase chain reaction (qPCR), and enzyme-linked immunosorbent assay (ELISA) were used to investigate the protective mechanisms of CXTB in joint cartilage. RESULTS: CXTB effectively improved the morphological structure of the cartilage and bone in the knee joint by enhancing autophagy and regulating the expression of related protease and inflammatory factors. Furthermore, CXTB downregulated the expression of the long non-coding RNA (lncRNA) Hox transcript antisense intergenic RNA (HOTAIR) and inhibited the activation of the p38MAPK pathway. Conversely, overexpression of lncRNA HOTAIR suppressed the protective effects of CXTB on the knee joint. CONCLUSIONS: CXTB capsules can protect the knee articular cartilage in rats through the lncRNA HOTAIR/p38MAPK pathway.

5.
J Orthop Surg Res ; 17(1): 105, 2022 Feb 19.
Artículo en Inglés | MEDLINE | ID: mdl-35183198

RESUMEN

BACKGROUND: The surgical protocol of ORIF for the treatment of mid-shaft clavicle fractures is common. However, poor plate fit or overhang usually occurs when the straight plate is selected for superior fixation. This is because the upper edge of the clavicle is not flat but has an angulation near the conoid tubercle. We termed that angulation, conoid tubercle angle (CTA). Supposed the straight plate is forcibly attached to the surface of the clavicle, it will potentially cause misalignment of the fracture end and with that comes a change of CTA. In this case, choosing the contoured plate, such as a commercial pre-contoured anatomic plate or manual-contoured plate, for superior fixation seems to meet the requirements for both plate fit and fracture alignment. Hence, we retrospectively compared the radiological parameters, including the plate overhang, and the alignment of the fractures reflected by the CTA, between the contoured plate (CP) and straight plate (SP) groups, to draw attention to the CTA and its effects to supraclavicular plate fixation. METHODS: From March 2018 to April 2021, 217 patients with clavicle fractures that met the inclusion criteria but not the exclusion criteria were included in our study. 112 patients were enrolled into the straight plate group (SP) and 105 patients into the contoured plate group (CP). Besides that, 154 healthy adults were recruited into the health group (HA). RESULTS: Patients were followed up for 6 to 40 months postoperative. A normal CTA (164.54 ± 4.78°) was obtained from the HA group. There were 50 cases with plate overhang in the SP group, which presented a statistical difference in comparison with the CP group. The value of CTA (169.65 ± 5.84°) in the SP group also indicated a statistical difference in comparison with the normal CTA. Subgroup analysis showed that the CTA (165.88 ± 5.42°) in the overhang subgroup (O) had no statistical difference in comparison with the normal CTA, but the CTA (172.68 ± 4.18°) in the non-overhang subgroup (N-O) had. 3 cases experienced non-traumatic re-fracture (within 3 months after the removal of the fixation) in the O subgroup; 10 cases experienced a poor reduction in the N-O subgroup. In the CP group, the CTA was 166.79 ± 5.68°, which indicated a statistical difference with the SP group. Subgroup analysis was performed, including the manual-contoured plate subgroup (M-C) and commercial pre-contoured anatomic plate subgroup (P-C). The value of CTA (M-C, 166.97 ± 6.33°; P-C, 166.44 ± 6.33°) manifested a statistical difference in comparison with the N-O subgroup. 2 and 8 cases, respectively, had screw loosening and poor reduction in the M-C subgroup. No postoperative complication occurred in the P-C subgroup. CONCLUSION: CTA is a useful reference in the evaluation of the reduction obtained on radiographic examination, and a reference guiding the plate contouring. The commercial pre-contoured anatomic plate provides a normal CTA and well fits the biomechanical characteristics of the clavicle, which can be recommended for superior fixation.


Asunto(s)
Placas Óseas , Clavícula/cirugía , Fijación Interna de Fracturas/métodos , Fracturas Óseas/cirugía , Adolescente , Adulto , Anciano , Clavícula/diagnóstico por imagen , Femenino , Fracturas Óseas/diagnóstico por imagen , Humanos , Masculino , Persona de Mediana Edad , Estudios Retrospectivos , Resultado del Tratamiento , Adulto Joven
6.
J Orthop Surg Res ; 16(1): 409, 2021 Jun 26.
Artículo en Inglés | MEDLINE | ID: mdl-34174925

RESUMEN

BACKGROUND: Percutaneous pinning fixation (PCP) has been used for the treatment of distal radius fractures for decades, especially in the elderly with fragile soft tissue. However, achieving and maintaining a sound anatomic reduction before PCP is difficult if we use the manipulative reduction method alone. Our study innovatively applied the Steinmann pin retractor for closed reduction combined with PCP, to provide a new protocol for the treatment of distal radius fractures. METHODS: From March 2017 to July 2018, 49 patients out of 57 that met the inclusion criteria but not the exclusion criteria were included in our retrospective cohort study. Sixteen patients were treated with Steinmann pin retractor-assisted closed reduction combined with PCP (S-PCP), and 19 patients were treated with the manipulative reduction combined with PCP (M-PCP), and 14 patients were treated with the manipulative reduction combined with cast splint (M-C). All these patients received a positive postoperative radiological and clinical evaluation. RESULTS: All the patients were followed up for a minimum of 2 years. The radiological parameters in each group improved significantly postoperative (posttreatment). In the S-PCP group, the values of radial height (postoperative, 13.33±1.74 mm; the first follow-up, 13.27±1.81mm; last follow-up, 13.16±1.76mm) and ulnar variance (postoperative, -0.10±1.29mm; the first follow-up, -0.05±1.27mm; last follow-up, -0.12±1.09mm) significantly improved as compared to the M-PCP and M-C groups. While the patients in the M-C group experienced significant re-displacement at the first and last follow-ups, in the S-PCP group, the range of wrist motion including extension (89.94±5.21%), radial deviation (90.69±6.01%), and supination (90.25±5.87%); ulnar deviation (89.81±5.82%) and QuickDASH score (2.70±3.64); and grip strength (92.50±5.59%), pronation (90.50±6.04%), and modified Mayo wrist score (90.94±4.17, the excellent rate reached up to 75%) also improved as compared to the M-PCP group, M-C group, or both groups at the last follow-up. CONCLUSION: S-PCP improves fracture reduction and wrist function and can serve as an effective method for A2(AO/OTA) and A3 type of distal radius fractures in the elderly with limited dorsal comminution, including intra-articular fractures with displacement less than 2mm.


Asunto(s)
Clavos Ortopédicos , Reducción Cerrada/instrumentación , Fijación Intramedular de Fracturas/instrumentación , Fracturas Intraarticulares/cirugía , Fracturas del Radio/cirugía , Anciano , Anciano de 80 o más Años , Reducción Cerrada/métodos , Femenino , Fijación Intramedular de Fracturas/métodos , Curación de Fractura , Humanos , Fracturas Intraarticulares/diagnóstico por imagen , Fracturas Intraarticulares/fisiopatología , Masculino , Persona de Mediana Edad , Radiografía , Radio (Anatomía)/diagnóstico por imagen , Radio (Anatomía)/fisiopatología , Fracturas del Radio/diagnóstico por imagen , Fracturas del Radio/fisiopatología , Estudios Retrospectivos , Férulas (Fijadores) , Resultado del Tratamiento
7.
Exp Ther Med ; 21(5): 468, 2021 May.
Artículo en Inglés | MEDLINE | ID: mdl-33767763

RESUMEN

The objective of the present study was to investigate the effect of quercetin and evaluate its protective effect on articular cartilage in patients with osteoarthritis (OA), by intervening the p38 pathway. The target factors of quercetin protecting articular cartilage in patients with OA were predicted scientifically and analyzed to predict the possible pathways by using network pharmacology. A pathway predicted to be closely associated with osteoarthritis was chosen for experimental verification in in vitro cells. The optimal intervention drug concentrations were selected by the of Cell Cycle Kit-8 assay, osteoarthritis and inflammatory factors relevant to osteoarthritis, interleukin-1ß and tumor necrosis factor-α, were tested by of enzyme-linked immunosorbent assay, and the expression of relevant proteins and mRNA of the p38 signaling pathway was tested by reverse transcription-quantitative PCR and western blotting, following quercetin intervention. It was found that quercetin, at the concentration of 100 umol/l, can decrease inflammatory factors relevant to OA, inhibit the expression of p38, matrix metalloprotease 13 and ADAMTS in the pathway, and promote the expression of collagen Ⅱ. Therefore, it is postulated that quercetin can lower the expression of inflammatory factors in cartilage for the prevention and treatment of OA, and the expression level of relevant factors can be changed positively by blocking the p38 MAPK signaling pathway. Thus, quercetin can promote the repair of degenerative chondrocytes and protect articular chondrocytes.

8.
Ann Transl Med ; 8(22): 1511, 2020 Nov.
Artículo en Inglés | MEDLINE | ID: mdl-33313256

RESUMEN

BACKGROUND: Knee osteoarthritis (KOA) is a disease with a high incidence in elderly patients and traditional Chinese medicine has a significant effect on the treatment of KOA. Cangxitongbi capsule (CXTB) is a traditional Chinese medicine for KOA treatment and has a remarkable curative effect. The purpose of this article is to investigate the mechanism of CXTB in protecting joint cartilage on KOA rats. METHODS: A total of 30 male Sprague-Dawley rats were randomly assigned into five groups: control group; model group; low-, mid-, and high-dose CXTB groups (17.5, 35, and 70 mg/mL). KOA models were made by modified Hulth method except the control group. After pharmacological administration for 4 weeks, knee articular cartilages were observed by hematoxylin and eosin (HE) staining and evaluated by Mankin score. Western blot and reverse transcription-polymerase chain reaction (RT-PCR) were used to detect the concentration of ADAMTS-5. The peripheral blood of the rats was collected to detect content of interleukin-1 beta (IL-1ß) and tumor necrosis factor-alpha (TNF-α) by enzyme-linked immunosorbent assay (ELISA). RESULTS: The morphological structure of cartilage in the 3 CXTB groups was significantly improved compared with the model group, and the improvement positively correlated with the drug dosage (P<0.05). Compared with the model group, the expression levels of ADAMTS-5 of the 3 CXTB groups was obviously decreased (P<0.05). Furthermore, the upstream targets of ADAMTS-5, including IL-1ß and TNF-α were down-regulated in the 3 CXTB groups (P<0.05). CONCLUSIONS: Knee joint cartilage on KOA model rats is protected by CXTB via down-regulation of ADAMTS-5. The upstream targets of ADAMTS-5, IL-1ß and TNF-α, were also down-regulated by CXTB.

9.
Medicine (Baltimore) ; 99(25): e20556, 2020 Jun 19.
Artículo en Inglés | MEDLINE | ID: mdl-32569177

RESUMEN

BACKGROUND: Knee osteoarthritis (KOA) is 1 of the commonest cause of disability with joint pain in adults and a burden on healthcare resources. The limitations of current KOA treatment necessitate further researches to discover the more efficacious and safety treatments. There are increasing clinical studies investigating the potential protective effects of Curcuminoids in the alleviation of symptoms in patients suffering from KOA. However, the convincing evidence indicating the efficacy of curcuminoids for patients suffering from KOA remains unclear. METHODS: Several databases including PubMed, Web of Science, Cochrane Library, Embase, Chinese Biomedical Literature Database, Chinese National Knowledge Infrastructure, and Wanfang Database will be searched. And the language was not limited. We will include all Randomized controlled trials that use curcuminoids to treat patients with KOA, regardless of blinding. If the pre-crossover data can be analyzed to avoid carryover effects, the crossover randomized trials also are included. Meanwhile, We will exclude non-randomized controlled trials, qualitative studies, uncontrolled clinical trials and laboratory studies. The primary end point include Western Ontario and McMaster Universities Osteoarthritis Index, visual analog scale scores and Lequesne's pain functional index. The secondary end points are total effective rate and adverse effects. The Review Manager Version 5.3 will be used to perform the data synthesis and subgroup analysis. DISCUSSION: There are evidences that supports the potential protective effects of Curcuminoids in the alleviation of symptoms in patients suffering from KOA. This systematic review and meta-analysis would provide convincing evidence indicating that curcuminoids relieve the symptoms of patients suffering from KOA. REGISTRATION: Open Science Framework (OSF) registries (https://osf.io/fz29b) with the registration DOI: 10.17605/OSF.IO/FZ29B.


Asunto(s)
Artralgia/tratamiento farmacológico , Diarilheptanoides/uso terapéutico , Osteoartritis de la Rodilla/tratamiento farmacológico , Extractos Vegetales/uso terapéutico , Adulto , Diarilheptanoides/farmacología , Humanos , Masculino , Metaanálisis como Asunto , Extractos Vegetales/farmacología , Revisiones Sistemáticas como Asunto
10.
Exp Ther Med ; 16(6): 5390-5393, 2018 Dec.
Artículo en Inglés | MEDLINE | ID: mdl-30542500

RESUMEN

The present study examined the effectiveness of the method of inversing the distal clavicle anatomic locking plate to treat medial-end clavicle fractures. This involved retrospectively analyzing six cases of medial-end clavicle fractures treated using the method of inversing the distal clavicle anatomic locking plate between September 2013 and December 2015. These cases included five men and one woman with an average age of 46.3±10.6 years old and a range of 24-66 years old. Among these cases, one was type A, two were type C, and three were type D, based on the Throckmorton type classification. All patients had fresh closed fractures, which were caused by direct injury. A regular postoperative follow-up was performed and the therapeutic efficacy was evaluated systematically with reference to the Rockwood and Disability of the Arm, Shoulder and Hand (DASH) score standards. All six cases had a follow-up visit, which was between 10 and 14 months with an average of 12.0±2.2 months. All patients exhibited osseous healing and functional recovery without incision infection, nerve vascular injury, chest injury, fracture nonunion, or other complications. According to the Rockwood and DASH score standards, evaluation of the curative effect showed that five cases were excellent and one case was good, and the associated rate for achieving good or excellent outcomes was 100%. All of the DASH scores were <10. These results indicated that the effect of the treatment of medial-end clavicle fractures by the method of inversing the distal clavicle anatomic locking plate was reliable, and the functional recovery of patients was good, making this method worthy of clinical promotion.

11.
Exp Ther Med ; 15(6): 4737-4742, 2018 Jun.
Artículo en Inglés | MEDLINE | ID: mdl-29844798

RESUMEN

Diallyl trisulfide (DATS) is a natural organic sulfur compound that may be isolated from garlic and has strong anticancer activity. DATS has been demonstrated to upregulate the expression of calreticulin (CRT) in various types of human cancers, which is associated with the prognosis of cancer and its response to therapy. However, whether DATS has the same effect on human osteosarcoma cells is not known. Therefore, in the present study, Saos-2 human osteosarcoma cells were cultured with different concentrations of DATS (0, 25, 50 and 100 µmol/l) for 24 h, or with 50 µmol/l DATS for different time periods (0, 12, 24 and 36 h). Reverse transcription-quantitative polymerase chain reaction (RT-qPCR), western blotting and immunofluorescent staining were used to detect CRT mRNA and protein in the Saos-2 cells. Exposure to DATS changed the morphology and inhibited the growth of the Saos-2 cells, and its effects appeared to be concentration- and exposure time-dependent. The optimum concentration and exposure time of DATS were 50 µmol/l and 24 h, respectively. The levels of CRT mRNA and protein in the Saos-2 cells were significantly upregulated following exposure to DATS. The upregulation of CRT expression by DATS may be a mechanism underlying the ability of DATS to inhibit the growth of human osteosarcoma Saos-2 cells.

12.
Oncotarget ; 8(33): 55489-55510, 2017 Aug 15.
Artículo en Inglés | MEDLINE | ID: mdl-28903436

RESUMEN

BACKGROUND: Previous articles have reported that expression levels of microRNAs (miRNAs) are associated with survival time of patients with gastric cancer (GC). A systematic review and meta-analysis was performed to study the outcome of it. DESIGN: Meta-analysis. METHODS: English studies estimating expression levels of miRNAs with any of survival curves in GC were identified up till March 19, 2017 through performing online searches in PubMed, EMBASE, Web of Science and Cochrane Database of Systematic Reviews by two authors independently. The pooled hazard ratios (HR) with 95% confidence intervals (CI) were used to estimate the correlation between miRNA expression and overall survival (OS). RESULTS: Sixty-nine relevant articles about 26 miRNAs with 6148 patients were ultimately included. GC patients with high expression of miR-20b (HR=2.38, 95%CI=1.16-4.87), 21 (HR=1.77, 95%CI=1.01-3.08), 106b (HR=1.84, 95%CI=1.15-2.94), 196a (HR=2.66, 95%CI=1.94-3.63), 196b (HR=1.67, 95%CI=1.38-2.02), 214 (HR=1.84, 95%CI=1.27-2.67) or low expression of miR-125a (HR=2.06, 95%CI=1.26-3.37), 137 (HR=3.21, 95%CI=1.68-6.13), 141 (HR=2.47, 95%CI=1.34-4.56), 145 (HR=1.62, 95%CI=1.07-2.46), 146a (HR=2.60, 95%CI=1.63-4.13), 206 (HR=2.85, 95%CI=1.73-4.70), 218 (HR=2.61, 95%CI=1.74-3.92), 451 (HR=1.73, 95%CI=1.19-2.52), 486-5p (HR=2.45, 95%CI=1.65-3.65), 506 (HR=2.07, 95%CI=1.33-3.23) have significantly poor OS (P<0.05). CONCLUSIONS: In summary, miR-20b, 21, 106b, 125a, 137, 141, 145, 146a, 196a, 196b, 206, 214, 218, 451, 486-5p and 506 demonstrate significantly prognostic value. Among them, miR-20b, 125a, 137, 141, 146a, 196a, 206, 218, 486-5p and 506 are strong biomarkers of prognosis in GC.

13.
Oncotarget ; 8(63): 107237-107257, 2017 Dec 05.
Artículo en Inglés | MEDLINE | ID: mdl-29291025

RESUMEN

BACKGROUND: Numerous articles reported that dysregulated expression levels of miRNAs correlated with survival time of HCC patients. However, there has not been a comprehensive meta-analysis to evaluate the accurate prognostic value of miRNAs in HCC. DESIGN: Meta-analysis. MATERIALS AND METHODS: Studies, published in English, estimating expression levels of miRNAs with any survival curves in HCC were identified up until 15 April, 2017 by performing online searches in PubMed, EMBASE, Web of Science and Cochrane Database of Systematic Reviews by two independent authors. The pooled hazard ratios (HR) with 95% confidence intervals (CI) were used to estimate the correlation between miRNA expression and overall survival (OS). RESULTS: 54 relevant articles about 16 miRNAs, with 6464 patients, were ultimately included. HCC patients with high expression of tissue miR-9 (HR = 2.35, 95% CI = 1.46-3.76), miR-21 (HR = 1.76, 95% CI = 1.29-2.41), miR-34c (HR = 1.64, 95% CI = 1.05-2.57), miR-155 (HR = 2.84, 95% CI = 1.46-5.51), miR-221 (HR = 1.76, 95% CI = 1.02-3.04) or low expression of tissue miR-22 (HR = 2.29, 95% CI = 1.63-3.21), miR-29c (HR = 1.35, 95% CI = 1.10-1.65), miR-34a (HR = 1.84, 95% CI = 1.30-2.59), miR-199a (HR = 2.78, 95% CI = 1.89-4.08), miR-200a (HR = 2.64, 95% CI = 1.86-3.77), miR-203 (HR = 2.20, 95% CI = 1.61-3.00) have significantly poor OS (P < 0.05). Likewise, HCC patients with high expression of blood miR-21 (HR = 1.73, 95% CI = 1.07-2.80), miR-192 (HR = 2.42, 95% CI = 1.15-5.10), miR-224 (HR = 1.56, 95% CI = 1.14-2.12) or low expression of blood miR-148a (HR = 2.26, 95% CI = 1.11-4.59) have significantly short OS (P < 0.05). CONCLUSIONS: In conclusion, tissue miR-9, miR-21, miR-22, miR-29c, miR-34a, miR-34c, miR-155, miR-199a, miR-200a, miR-203, miR-221 and blood miR-21, miR-148a, miR-192, miR-224 demonstrate significantly prognostic value. Among them, tissue miR-9, miR-22, miR-155, miR-199a, miR-200a, miR-203 and blood miR-148a, miR-192 are potential prognostic candidates for predicting OS in HCC.

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