Quantitative evaluation and the toxicity mechanism of synergism within three organophosphorus pesticide mixtures to Chlorella pyrenoidosa.

Organophosphorus pesticide (OPP) pollutants in the environment pose toxicity risks to living organisms, and the possible toxicity mechanism needs to be further clarified. Therefore, the individual and combined toxicity of three OPPs namely acephate (ACE), trichlorfon (TRI) and glyphosate (GIY) towards a freshwater green alga Chlorella pyrenoidosa (C. pyrenoidosa) was investigated by the time-dependent microplate toxicity analysis method. Here, a ternary mixture system of the three OPPs including five rays with different concentration ratios was designed by the uniform design ray method. The standard additive reference model, concentration addition (CA), was used to analyse toxicity interaction within ternary mixtures and the toxicity interaction intensity was characterized using a deviation from CA model (dCA). Besides, the effects of the three OPPs and their mixtures on the chlorophyll (CHL) content, superoxide dismutase (SOD) activity and malondialdehyde (MDA) content of C. pyrenoidosa were also investigated to explore the possible mechanisms. The results show that toxicity of the three pesticides and their ternary mixture rays is time-dependent and the combined toxicity correlates well with the components, ACE and GLY. It is likely that there is a significant time-dependent synergism in ternary mixtures induced by ACE and GLY. The synergism intensity of the ternary mixtures is not more than 30% at the whole experimental concentration level. The CHL reduction rate and MDA content of C. pyrenoidosa increase, while the SOD activity of C. pyrenoidosa decreases with the lengthening of exposure time under the action of the three pesticides and their ternary mixtures. So, the possible mechanism of the three pesticides and their mixtures may be by affecting the photosynthesis, and then causing oxidative damage to C. pyrenoidosa cells. The results can provide reference for the combined toxicity assessment of OPPs to living organisms.

Time-dependent and Pb-dependent antagonism and synergism towards Vibrio qinghaiensis sp.-Q67 within heavy metal mixtures.

Toxicity interaction has aroused many researchers' interest in the combined toxicity of pollutants. Recently, some published studies have shown that the toxicity of some mixture pollutants is time dependent and well correlated with certain components in the mixture. Therefore, to investigate whether toxicity interaction is affected by the exposure time or certain components, synergism and antagonism within typical environmental contaminants of heavy metal mixtures were analyzed in different exposure times. Herein, three binary and one ternary mixture systems were designed by using three heavy metals: cadmium chloride, lead chloride (Pb) and manganese(ii) chloride tetrahydrate (Mn). For each mixture system, five mixture rays with different concentration ratios were arranged by direct equipartition ray design and uniform design ray methods. The toxicities of the three heavy metals and 20 mixture rays towards photobacteria Vibrio qinghaiensis sp.-Q67 (Q67) were determined by the established time-dependent microplate toxicity analysis (t-MTA) in different exposure durations of 0.25, 2, 4, 8 and 12 h. It was shown that the toxicities of three heavy metals (Cd, Pb and Mn) as well as their binary and ternary mixture rays to Q67 were also time dependent, but different metals or mixture rays showed different time characteristics. Surprisingly, some mixture rays exhibited antagonism or synergism with time dependency and the time characteristics varied in different mixture systems. Furthermore, the binary or ternary mixture systems with Pb displayed antagonism, while the Cd-Mn mixture system without Pb exhibited additive action or synergism, which indicated that Pb was probably the causative agent of antagonism produced by mixtures.