Mobility of crocidolite asbestos in sandy porous media mimicking aquifer systems.

Asbestos is widely recognized as being a carcinogen when dispersed in air, but very little is known about its exposure pathways in water and its subsequent effects on human health. Several studies have proved asbestos presence in groundwater but failed to assess its mobility in aquifer systems. This paper aims to fill this gap by studying the transport of crocidolite, an amphibole asbestos, through sandy porous media mimicking different aquifer systems. To this purpose, two sets of column test were performed varying the crocidolite suspension concentration, the quartz sand grain size distribution, and the physicochemical water parameters (i.e., pH). The results proved that crocidolite is mobile in quartz sand due to the repulsive interactions between fibres and porous media. The concentration of fibres at the outlet of the column were found to decrease when decreasing the grain size distribution of the porous medium, with a bigger impact on highly concentrated suspensions. In particular, 5-to-10-µm-long fibres were able to flow through all the tested sands while fibres longer than 10 µm were mobile only through the coarser medium. These results confirm that groundwater migration should be considered a potential exposure pathway while implementing human health risk assessment.

A pore-scale investigation of the effect of nanoparticle injection on properties of sandy porous media.

Nanoremediation is a new groundwater remediation technology in which nanoparticles (NPs) are injected into the sub-surface to promote in-situ degradation of aquifer contaminants. Although nanoremediation is an effective process to eliminate contaminants in-situ, its success relies on sufficiently mobile NPs that can reach the contaminated zones and remain there to facilitate chemical degradation of contaminants. Therefore, understanding the main parameters that control the mobility and retention of NPs in saturated porous media is a key component of designing a successful nanoremediation process. This work presents the outcome of a pore-scale study of nZVI NP (zero-valent iron) transport in sandy porous media using the non-destructive 3D imaging technique, X-ray computed micro-tomography (X-ray micro-CT). We investigate the effect of grain size (fine, coarse, carbonate and mixed sand) and composition (carbonate vs sand grains) on the mobility and retention of NPs in sand columns. To achieve this, we used four columns packed with grains of different sizes and compositions. The main contribution of this work is, therefore, to understand the effect of NP injection on the structural and geometric properties of sandy porous media and to identify the main pore-scale mechanisms controlling NP transport and entrapment. Our experiment shows that the pore geometries change because of NP injection. Pore clogging is evidenced through pore size and throat size distribution displaying a shift to the left with a noticeable reduction in pore connectivity in all the columns. The porosity and permeability of the columns studied display significant reduction as result of the NP injection.

Nanoplastics removal during drinking water treatment: Laboratory- and pilot-scale experiments and modeling.

Microplastics detected in potable water sources and tap water have led to concerns about the efficacy of current drinking water treatment processes to remove these contaminants. It is hypothesized that drinking water resources contain nanoplastics (NPs), but the detection of NPs is challenging. We, therefore, used palladium (Pd)-labeled NPs to investigate the behavior and removal of NPs during conventional drinking water treatment processes including ozonation, sand and activated carbon filtration. Ozone doses typically applied in drinking water treatment plants (DWTPs) hardly affect the NPs transport in the subsequent filtration systems. Amongst the different filtration media, NPs particles were most efficiently retained when aged (i.e. biofilm coated) sand was used with good agreements between laboratory and pilot scale systems. The removal of NPs through multiple filtration steps in a municipal full-scale DWTP was simulated using the MNMs software code. Removal efficiencies exceeding 3-log units were modeled for a combination of three consecutive filtration steps (rapid sand filtration, activated carbon filtration and slow sand filtration with 0.4-, 0.2- and 3.0-log-removal, respectively). According to the results from the model, the removal of NPs during slow sand filtration dominated the overall NPs removal which is also supported by the laboratory-scale and pilot-scale data. The results from this study can be used to estimate the NPs removal efficiency of typical DWTPs with similar water treatment chains.

Exploring the potential of graphene oxide nanosheets for porous media decontamination from cationic dyes.

Graphene oxide (GO) nanosheets, often embedded in nano-composites, have been studied as promising materials for waste water purification, in particular to adsorb heavy metals and cationic organic contaminants. However, a broader range of potential applications of GO is still unexplored. This work investigated the potential applicability of GO for enhanced in-situ soil washing of secondary sources of groundwater contamination (i.e. the controlled recirculation of a washing GO suspension via injection/extraction wells). The laboratory study aimed at quantifying the capability of GO to effectively remove adsorbed methylene blue (MB) from contaminated sand. The tests were conducted in simplified conditions (synthetic groundwater at NaCl concentration of 20 mM, silica sand) to better highlight the key mechanisms under study. The results indicated a maximum sorption capacity of 1.6 mgMB/mgGO in moderately alkaline conditions. Even though the adsorption of MB onto GO slightly reduced the GO mobility in the porous medium, a breakthrough higher than 95% was obtained for MB/GO mass ratios up to 0.5. This suggests that a very high recovery of the injected particles should be also expected in the field.

Natural clay and biopolymer-based nanopesticides to control the environmental spread of a soluble herbicide.

In this work a novel nano-formulation is proposed to control leaching and volatilization of a broadly used herbicide, dicamba. Dicamba is subject to significant leaching in soils, due to its marked solubility, and to significant volatilization and vapor drift, with consequent risks for operators and neighbouring crops. Natural, biocompatible, low-cost materials were employed to control its dispersion in the environment: among four tested candidate carriers, a nanosized natural clay (namely, K10 montmorillonite) was selected to adsorb the pesticide, and carboxymethyl cellulose (CMC), a food-grade biodegradable polymer, was employed as a coating agent. The synthesis approach is based on direct adsorption at ambient temperature and pressure, with a subsequent particle coating to increase suspension stability and control pesticide release. The nano-formulation showed a controlled release when diluted to field-relevant concentrations: in tap water, the uncoated K10 released approximately 45% of the total loaded dicamba, and the percentage reduced to less than 30% with coating. CMC also contributed to significantly reduce dicamba losses due to volatilization from treated soils (e.g., in medium sand, 9.3% of dicamba was lost in 24 h from the commercial product, 15.1% from the uncoated nanoformulation, and only 4.5% from the coated one). Moreover, the coated nanoformulation showed a dramatic decrease in mobility in porous media (when injected in a 11.6 cm sand-packed column, 99.3% of the commercial formulation was eluted, compared to 88.4% of the uncoated nanoformulation and only 24.5% of the coated one). Greenhouse tests indicated that the clay-based nanoformulation does not hinder the dicamba efficacy toward target weeds, even though differences were observed depending on the treated species. Despite the small (lab and greenhouse) scale of the tests, these preliminary results suggest a good efficacy of the proposed nanoformulation in controlling the environmental spreading of dicamba, without hindering efficacy toward target species.

Pilot Investigation of Anti-Salmonella Antibodies in Oral Fluids from Salmonella Typhimurium Vaccinated and Unvaccinated Swine Herds.

Oral fluid (OF) can be a simple, cheap and non-invasive alternative to serum or meat juice for the diagnosis and surveillance of important pathogens in pigs. This study was conducted on four Salmonella Typhimurium-positive farrow-to-finish pig farms: two Salmonella-vaccinated (V) and two non-vaccinated (NV). Gilts and sows in the V farms were vaccinated with a live, attenuated vaccine prior to farrowing. Pooled faecal and OF samples were collected from the sows and their offspring. Salmonella was isolated according to ISO6579-1:2017. In parallel, IgG and IgA levels were assessed in OF samples using a commercial ELISA assay. Salmonella was detected in 90.9% of the pooled faecal samples from the NV farms and in 35.1% of the pooled faecal samples from the V farms. Overall, a higher prevalence was observed in the pooled faecal samples from the offspring (76.3%) compared to the sows (36.4%). IgG antibodies measured in V farms are likely to be related to vaccination, as well as exposure to Salmonella field strains. The detection of IgA antibodies in OF was unreliable with the method used. The results of this study show that IgG is the most reliable isotype for monitoring Salmonella-specific antibody immunity in vaccinated/infected animals via OF.

Canine Placenta Histological Findings and Microvascular Density: The Histological Basis of a Negative Neonatal Outcome?

Placenta is essential for the development of the fetus, and its impaired function can lead to a negative outcome (i.e., neonatal mortality). In dogs, investigations on placenta histology and neonatal outcome in healthy bitches are lacking, and a contribution is provided in this study to emphasize the use of placenta histology in practice. Fifty-one placentas from 11 litters were collected during cesarean section, classified according to the litter size (large (L) or small (S)) and the outcome, this latter as healthy (Group 1) or dead within 7 days (Group 2). The placenta/puppy weight ratio (PPR) was calculated, and specimens were formalin-fixed and paraffin-wax embedded, and on the resulting histological slides, capillary density (CD) was quantified. Among necrosis, calcification, and intravascular leucocytes, only the presence of multifocal-confluent necrosis (significantly more frequent in Group 2) was associated with a higher risk of death within 7 days (odds ratio = 30.7). Mixed logistic regression ruled out the effect on death both of a bitch and cesarean type (programmed vs. emergency). PPR and CD values were associated with litter size; large litters had lower PPR (p < 0.01) and higher CD (p < 0.05) than small litters. The relationship between PPR and CD was negative and significant (p < 0.01). Necrosis was a frequent finding in canine placentas, but only when multifocal-confluent was it associated with a poor outcome. The litter size influenced PPR (lower in L) and CD (higher in L), and this is likely due to the plasticity of placenta adaptation.

Non-exhaust traffic emissions: Sources, characterization, and mitigation measures.

Non-exhaust emissions (NEE) of particulate matter (PM) from brake, tyre, road pavement and railway wear, as well as resuspension of already deposited road dust, account for up to 90% by mass of total traffic-related PM emitted. This review aims at analysing the current knowledge on road traffic NEE regarding sources, particle generation processes, chemical and physical characterization, and mitigation strategies. The literature on this matter often presents highly variable and hardly comparable results due to the heterogeneity of NEE sources and the absence of standardized sampling and measurement protocols. As evidence, emission factors (EFs) were found to range from 1 mg km-1 veh-1 to 18.5 mg km-1 veh-1 for brake wear, and from 0.3 mg km-1 veh-1 to 7.4 mg km-1 veh-1 for tyre wear. Resuspended dust, which varies in even wider ranges (from 5.4 mg km-1 veh-1 to 330 mg km-1 veh-1 for cars), is considered the prevailing NEE source. The lack of standardized monitoring approaches resulted in the impossibility of setting international regulations to limit NEE. Therefore, up until now the abatement of NEE has only been achieved by mitigation and prevention strategies. However, the effectiveness of these measures still needs to be improved and further investigated. As an example, mitigation strategies, such as street washing or sweeping, proved effective in reducing PM levels, but only in the short term. The replacement of internal combustion engines vehicles with electric ones was instead proposed as a prevention strategy, but there are still concerns regarding the increase of NEE deriving from the extra weight of the batteries. The data reported in this review highlighted the need for future studies to broaden their research area, and to focus not only on the standardization of methods and the introduction of regulations, but also on improving already existing technologies and mitigating strategies.

Field-scale demonstration of in situ immobilization of heavy metals by injecting iron oxide nanoparticle adsorption barriers in groundwater.

Remediation of heavy metal-contaminated aquifers is a challenging process because they cannot be degraded by microorganisms. Together with the usually limited effectiveness of technologies applied today for treatment of heavy metal contaminated groundwater, this creates a need for new remediation technologies. We therefore developed a new treatment, in which permeable adsorption barriers are established in situ in aquifers by the injection of colloidal iron oxides. These adsorption barriers aim at the immobilization of heavy metals in aquifers groundwater, which was assessed in a large-scale field study in a brownfield site. Colloidal iron oxide (goethite) nanoparticles were used to install an in situ adsorption barrier in a very heterogeneous, contaminated aquifer of a brownfield in Asturias, Spain. The groundwater contained high concentrations of heavy metals with up to 25 mg/L zinc, 1.3 mg/L lead, 40 mg/L copper, 0.1 mg/L nickel and other minor heavy metal pollutants below 1 mg/L. High amounts of zinc (>900 mg/kg), lead (>2000 mg/kg), nickel (>190 mg/kg) were also present in the sediment. Ca. 1500 kg of goethite nanoparticles of 461 ± 266 nm diameter were injected at low pressure (< 0.6 bar) into the aquifer through nine screened injection wells. For each injection well, a radius of influence of at least 2.5 m was achieved within 8 h, creating an in situ barrier of 22 × 3 × 9 m. Despite the extremely high heavy metal contamination and the strong heterogeneity of the aquifer, successful immobilization of contaminants was observed in the tested area. The contaminant concentrations were strongly reduced immediately after the injection and the abatement of the heavy metals continued for a total post-injection monitoring period of 189 days. The iron oxide particles were found to adsorb heavy metals even at pH-values between 4 and 6, where low adsorption would have been expected. The study demonstrated the applicability of iron oxide nanoparticles for installing adsorption barriers for containment of heavy metals in contaminated groundwater under real conditions.

Detection of H3N8 influenza A virus with multiple mammalian-adaptive mutations in a rescued Grey seal (Halichoerus grypus) pup.

Avian influenza A viruses (IAVs) in different species of seals display a spectrum of pathogenicity, from sub-clinical infection to mass mortality events. Here we present an investigation of avian IAV infection in a 3- to 4-month-old Grey seal (Halichoerus grypus) pup, rescued from St Michael's Mount, Cornwall in 2017. The pup underwent medical treatment but died after two weeks; post-mortem examination and histology indicated sepsis as the cause of death. IAV NP antigen was detected by immunohistochemistry in the nasal mucosa, and sensitive real-time reverse transcription polymerase chain reaction assays detected trace amounts of viral RNA within the lower respiratory tract, suggesting that the infection may have been cleared naturally. IAV prevalence among Grey seals may therefore be underestimated. Moreover, contact with humans during the rescue raised concerns about potential zoonotic risk. Nucleotide sequencing revealed the virus to be of subtype H3N8. Combining a GISAID database BLAST search and time-scaled phylogenetic analyses, we inferred that the seal virus originated from an unsampled, locally circulating (in Northern Europe) viruses, likely from wild Anseriformes. From examining the protein alignments, we found several residue changes in the seal virus that did not occur in the bird viruses, including D701N in the PB2 segment, a rare mutation, and a hallmark of mammalian adaptation of bird viruses. IAVs of H3N8 subtype have been noted for their particular ability to cross the species barrier and cause productive infections, including historical records suggesting that they may have caused the 1889 pandemic. Therefore, infections such as the one we report here may be of interest to pandemic surveillance and risk and help us better understand the determinants and drivers of mammalian adaptation in influenza.

Key factors affecting graphene oxide transport in saturated porous media.

This study focuses on the transport in porous media of graphene oxide nanoparticles (GONP) under conditions similar to those applied in the generation of in-situ reactive zones for groundwater remediation (i.e. GO concentration of few tens of mg/l, stable suspension in alkaline solution). The experimental tests evaluated the influence on GO transport of three key factors, namely particle size (300-1200 nm), concentration (10-50 mg/L), and sand size (coarse to fine). Three sources of GONP were considered (two commercial and one synthesized in the laboratory). Particles were stably dispersed in water at pH 8.5 and showed a good mobility in the porous medium under all experimental conditions: after injection of 5 pore volumes and flushing, the highest recovery was around 90%, the lowest around 30% (only for largest particles in fine sand). The particle size was by far the most impacting parameter, with increasing mobility with decreasing size, even if sand size and particle concentration were also relevant. The source of GONP showed a minor impact on the mobility. The transport test data were successfully modeled using the advection-dispersion-deposition equations typically applied for spherical colloids. Experimental and modeling results suggested that GONP, under the explored conditions, are retained due to both blocking and straining, the latter being relevant only for large particles and/or fine sand. The findings of this study play a key role in the development of an in-situ groundwater remediation technology based on the injection of GONP for contaminant degradation or sorption. Despite their peculiar shape, GONP behavior in porous media is comparable with spherical colloids, which have been more studied by far. In particular, the possibility of modeling GONP transport using existing models ensures that they can be applied also for the design of field-scale injections of GONP, similarly to other particles already used in nanoremediation.

Demodicosis in a captive African straw-coloured fruit bat (Eidolon helvum).

Demodicosis is most frequently observed in the domestic dog (Canis familiaris), but it has rarely been reported in bats (Chiroptera). The overpopulation of Demodex spp. that causes dermatological changes is generally associated with a compromised immune system. We describe the gross and histological features of generalized demodicosis in an adult female African straw-coloured fruit bat (Eidolon helvum) drawn from a captive research colony. The histology of the lesions revealed comedones and follicular infundubular cysts harbouring numerous Demodex spp. mites, eliciting a minimal inflammatory response in the adjacent dermis. The histological examination of a full set of tissues did not reveal clear evidence of immunosuppression, although a clinical history of recent abortion and possible stressors due to captivity could be considered risk factors for the demodicosis. Attempts to determine the Demodex species using PCR on DNA extracted from the formalin fixed paraffin embedded tissue failed. This is the first clinical and histological description of demodicosis in Eidolon helvum.

Ducks Are Susceptible to Infection with a Range of Doses of H5N8 Highly Pathogenic Avian Influenza Virus (2016, Clade 2.3.4.4b) and Are Largely Resistant to Virus-Specific Mortality, but Efficiently Transmit Infection to Contact Turkeys.

Widespread H5N8 highly pathogenic avian influenza virus (HPAIV; clade 2.3.4.4b) infections occurred in wild birds and poultry across Europe during winter 2016-17. Four different doses of H5N8 HPAIV (A/wigeon/Wales/052833/2016 [wg-Wal-16]) were used to infect 23 Pekin ducks divided into four separate pens, with three contact turkeys introduced for cohousing per pen at 1 day postinfection (dpi). All doses resulted in successful duck infection, with four sporadic mortalities recorded among the 23 (17%) infected ducks, which appeared unrelated to the dose. The ducks transmitted wg-Wal-16 efficiently to the contact turkeys; all 12 (100%) turkeys died. Systemic viral dissemination was detected in multiple organs in two duck mortalities, with limited viral dissemination in another duck, which died after resolution of shedding. Systemic viral tropism was observed in two of the turkeys. The study demonstrated the utility of Pekin ducks as surrogates of infected waterfowl to model the wild bird/gallinaceous poultry interface for introduction of H5N8 HPAIV into terrestrial poultry, where contact turkeys served as a susceptible host. Detection of H5N8-specific antibody up to 58 dpi assured the value of serologic surveillance in farmed ducks by hemagglutination inhibition and anti-nucleoprotein ELISAs.

Los patos son susceptibles a la infección con un rango de dosis del virus de la influenza aviar altamente patógena subtipo H5N8 (2016, clado 2.3.4.4b) son muy resistentes a la mortalidad específica por el virus, pero transmiten la infección de manera eficiente a pavos por contacto. La diseminación de la infección por el virus de la influenza aviar altamente patógeno H5N8 (con las siglas en inglés HPAIV); clado 2.3.4.4b ocurrió en aves silvestres y avicultura comercial en toda Europa durante el invierno 2016­17. Se usaron cuatro dosis diferentes del virus de alta patogenicidad H5N8 (A/wigeon/Gales/052833/2016 [wg-Wal-16]) para infectar a 23 patos Pekin divididos en cuatro corrales, cohabitando con tres pavos en el corral para determinar transmisión por contacto al primer día después de la infección (dpi). Todas las dosis dieron como resultado una infección exitosa de los patos, con mortalidad esporádica en cuatro aves (17%) registradas entre los 23 patos infectados, que no parecieron estar relacionadas con la dosis. Los patos transmitieron el virus wg-Wal-16 de manera eficiente a los pavos por contacto; los 12 pavos (100%) murieron. La diseminación viral sistémica se detectó en múltiples órganos en dos patos muertos, con diseminación viral limitada en otro pato que murió después de la resolución de la eliminación viral. Se observó tropismo viral sistémico en dos de los pavos. El estudio demostró la utilidad de los patos Pekin como sustitutos de las aves acuáticas infectadas en un modelo de la interface entre aves silvestres y aves comerciales gallináceas para la introducción del subtipo H5N8 del virus de influenza aviar de alta patogenicidad en las aves comerciales terrestres. Los pavos de contacto sirvieron como hospedadores susceptibles. La detección de anticuerpos específicos contra el subtipo H5N8 hasta 58 días después de la inoculación justificó el valor de la vigilancia serológica en las granjas de patos mediante la inhibición de la hemaglutinación y por estuches de ELISA dirigidos contra la nucleoproteína.

Correlation of Anti-Salmonella Antibodies Between Serum and Saliva Samples Collected From Finisher Pigs.

Saliva samples obtained by using absorptive devices, can provide an alternative diagnostic matrix to serum for monitoring disease status in pigs. The aim of this study was to investigate the correlation of anti-Salmonella antibodies between serum and saliva samples collected from pigs. Twenty individual paired serum and saliva samples were collected from a single farm. Anti-Salmonella IgG was detected in individual serum samples using a commercial Salmonella ELISA kit, validated for sera. The same kit was used with a protocol modified by extending incubation time and increasing temperature to test individual saliva samples. Anti-Salmonella IgG antibodies in pig saliva were always detected at a lower level than in the matching serum samples. A correlation (rho = 0.66; p = 0.002) and a moderate agreement (K > 0.62 p = 0.003) was found between individual Salmonella IgG in serum and saliva samples. Both correlation and the agreement levels are moderate. The size of this investigation was small, and further studies are necessary to further confirm these findings. The results of this work provide some evidence that saliva samples have the potential to be used for the diagnosis of Salmonella infection in pig farms.

Controlled Deposition of Particles in Porous Media for Effective Aquifer Nanoremediation.

In this study, a model assisted strategy is developed to control the distribution of colloids in porous media in the framework of nanoremediation, an innovative environmental nanotechnology aimed at reclaiming contaminated aquifers. This approach is exemplified by the delivery of humic acid-stabilized iron oxide nanoparticles (FeOx), a typical reagent for in situ immobilization of heavy metals. By tuned sequential injections of FeOx suspensions and of solutions containing a destabilizing agent (i.e. calcium or magnesium), the two fronts, which advance at different rates, overlap at the target location (i.e., the central portion) of the porous systems. Here, the particles deposit and accumulate irreversibly, creating a reactive zone. An analytical expression predicting the position of the clustering zone in 1D systems is derived from first principles of advective-dispersive transport. Through this equation, the sequence and duration of the injection of the different solutions in the medium is assessed. The model robustness is demonstrated by its successful application to various systems, comprising the use of different sands or immobilizing cations, both in 1D and 2D geometries. The method represents an advancement in the control of nanomaterial fate in the environment, and could enhance nanoremediation making it an effective alternative to more conventional techniques.

Hernia of the swim bladder (aerocystocele) with concurrent mycotic granulomatous inflammation and swim bladder carcinoma in a wild mullet (Mugil cephalus).

We describe a hernia of the swim bladder, with a concurrent mycotic granulomatous inflammation, and carcinoma of the swim bladder in a wild mullet (Mugil cephalus) referred for an exophytic dorsal mass. Grossly, the mass was white, soft, and composed of multiple cystic gas-containing chambers connected by a funnel-shaped tissue segment to the coelomic swim bladder. Histologically, the mass was characterized by cysts of variable size, multifocally contiguous with the subepithelial rete mirabile, supported by abundant fibrous tissue. The skin covering the herniated swim bladder was focally ulcerated and replaced by abundant granulation tissue in which multiple scattered granulomas centered on pigmented fungal hyphae were observed. These granulomas were also seen in the remaining coelomic portion of the swim bladder as well as in the spleen, perivisceral pancreas, and peritoneal adipose tissue; the fungus was molecularly identified as Cladosporium spp. Focally, arising from the herniated swim bladder epithelium, an unencapsulated poorly demarcated, moderately cellular neoplasm, composed of islands, lobules, and acini of neoplastic epithelium, was found.

A 3-dimensional micro- and nanoparticle transport and filtration model (MNM3D) applied to the migration of carbon-based nanomaterials in porous media.

Engineered nanoparticles (NPs) in the environment can act both as contaminants, when they are unintentionally released, and as remediation agents when injected on purpose at contaminated sites. In this work two carbon-based NPs are considered, namely CARBO-IRON®, a new material developed for contaminated site remediation, and single layer graphene oxide (SLGO), a potential contaminant of the next future. Understanding and modeling the transport and deposition of such NPs in aquifer systems is a key aspect in both cases, and numerical models capable to simulate NP transport in groundwater in complex 3D scenarios are necessary. To this aim, this work proposes a modeling approach based on modified advection-dispersion-deposition equations accounting for the coupled influence of flow velocity and ionic strength on particle transport. A new modeling tool (MNM3D - Micro and Nanoparticle transport Model in 3D geometries) is presented for the simulation of NPs injection and transport in 3D scenarios. MNM3D is the result of the integration of the numerical code MNMs (Micro and Nanoparticle transport, filtration and clogging Model - Suite) in the well-known transport model RT3D (Clement et al., 1998). The injection in field-like conditions of CARBO-IRON® (20g/l) amended by CMC (4g/l) in a 2D vertical tank (0.7×1.0×0.12m) was simulated using MNM3D, and compared to experimental results under the same conditions. Column transport tests of SLGO at a concentration (10mg/l) representative of a possible spill of SLGO-containing waste water were performed at different values of ionic strength (0.1 to 35mM), evidencing a strong dependence of SLGO transport on IS, and a reversible blocking deposition. The experimental data were fitted using the numerical code MNMs and the ionic strength-dependent transport was up-scaled for a full scale 3D simulation of SLGO release and long-term transport in a heterogeneous aquifer. MNM3D showed to potentially represent a valid tool for the prediction of the long-term behavior of engineered nanoparticles released in the environment (e.g. from landfills), and the preliminary design of in situ aquifer remediation through injection of suspensions of reactive NPs.