Theromyzon maculosum (Rathke, 1862) as a vector of potentially pathogenic fungi in aquatic ecosystems.

The aim of the study was to check the integument and alimentary tract of Theromyzon for the occurrence of fungi. Moreover, the mycological purity of water sources from which leeches were collected was analyzed. The potential role of the duck leech Theromyzon maculosum in transmitting pathogenic fungi has not been studied until now. Mycological analyses were performed according to the procedure approved in mycological diagnostic laboratories, using classical diagnostic methods (macro- and microcultures, microscopic observations). In total, 22 species of yeasts were detected. The specimens belong to eight genera: Candida, Citeromyces, Debaryomyces, Kluyveromyces, Lodderomyces, Pichia, Saccharomyces and Saccharomycodes. Overall, 18 species of fungi were isolated from leeches Theromyzon maculosum, including seven species from the integument and 11 species from the gastrointestinal system. Among fungi isolated from body integument, fungi of the genus Candida were dominant, whereas in the gastrointestinal system, the genus Saccharomyces dominated. From the river waters, nine fungi species were isolated, and yeasts of the genera Candida and Kluyveromyces dominated. Isolation of the same species of microfungi from the integument of leeches and the river water suggests that Theromyzon maculosum may be a potential vector of pathogenic yeasts for vertebrates living in the same ecosystems and for humans.

Sucking of human blood by Placobdella costata (O. F. Müller, 1846) (Hirudinida: Glossiphoniidae): Case study with notes on body form.

Four events of Placobdella costata sucking human blood are described.Human blood was sucked by both adult and juvenile specimens of P. costata.The feeding strategies of juveniles under parental care are presented.New data on juvenile specimens' body form are presented.Information on the potential role of mammals in dispersion and habitat preferences of leeches P. costata is considered.

New record of the fish leech Piscicola pojmanskae (Annelida: Hirudinida: Piscicolidae) - DNA barcoding and phylogeny.

The aim of this study was to confirm the taxonomic status of Piscicola pojmanskae Bielecki, 1994 found on Salmonidae fish. The fish leech was identified based on a diligent morphological analysis as well as COI gene sequence (DNA barcoding). The phylogenetic relationship with other piscicolid leeches was analyzed as well. Piscicola pojmanskae was found on various fins of both graylings and the resident form of trouts. The prevalence of infection was 1.63%. In this paper, probable causes of the lack of relation between the number of leeches on the fins and the fish body length as well as the host-searching strategy used by P. pojmanskae are discussed.

A new leech species (Hirudinida: Erpobdellidae: Erpobdella) from a cave in the West Azerbaijan province of Iran.

Erpobdella borisi n. sp. is a predatory leech inhabiting cave waters in Iran. Probably, it is either a troglobiont or troglophile. The leech has no eyes, and the complete mid-body somite is divided unequally into five annuli. Results of phylogenetic analysis based on morphological characters and COI gene sequence indicate the species to be closely related to Erpobdella japonica, E. octoculata and E. testacea.

New data about the functional morphology of the chaetiferous leech-like annelids Acanthobdella peledina (Grube, 1851) and Paracanthobdella livanowi (Epshtein, 1966) (Clitellata, Acanthobdellida).

Approximately 130 individuals of Acanthobdella peledina and 100 individuals of Paracanthobdella livanowi were studied. Morphometric measurements were taken to explore the body form. The digestive and the reproductive systems of leech-like annelids were analyzed for the first time in such a high number of specimens. Observation of A. peledina and P. livanowi revealed crucial differences in the reproductive system of the analyzed taxa, mostly regarding variation in the shape of the testisacs and the length of the ovisacs. The results of a digestive system analysis suggest that blood-sucking parasites of the order Acanthobdellida may also lead a predatory lifestyle. The presented findings support the taxonomic division of the order Acanthobdellida into the families Acanthobdellidae and Paracanthobdellidae. Multiple similarities between Acanthobdellida and Hirudinida were also discussed.

Diversity of features of the female reproductive system and other morphological characters in leeches (Citellata, Hirudinida) in phylogenetic conception.

An epistemological-evolutionary conception of leeches (Hirudinida) based on features of the female reproductive system in combination with other morphological characters is presented in the spirit of the cladistic school of taxonomy. Characters relating to the structure of the ovary and the course of oogenesis in leeches were interpreted in this manner, for the first time. Each study was conducted on type species of higher taxonomic groups of true leeches. Results of analyses using features of the reproductive system only as well as in combination with other morphological characters show Piscicolidae and Glossiphoniidae as sister clades making Rhynchobdellida a monophyletic group. Also, Hirudiniformes and Erpobdelliformes appeared to be sister clades within Arhynchobdellida. The relationship between the outgroup specimens and leeches remained unresolved, because both Acanthobdella peledina and branchiobdellidans appeared to be in an equivocal relationship to hirudinidans. Characters concerning the structure of the female reproductive system and course of oogenesis thus appeared to be useful, although conservative, for reconstruction of leech phylogeny, and they well reflect phylogenetic relationships of Hirudinida at the family level.

A new leech species (Clitellata: Hirudinida: Piscicolidae) from the Lyna River near Olsztyn, Poland.

A new species of fish leech from Poland, Piscicola burresoni n. sp. (Piscicolidae), is described. The leech was found in 2007 on stones and on pike ( Esox lucius L.) and chub ( Leuciscus cephalus L.) in the Lyna River, Poland. The leech is small, relatively flattened, with a prominent coarctation between the trachelosome and urosome. The pigmentation is unique to the new species and it has 14 annuli with gonopores separated by 4 annuli. The receptaculum seminis consists of an elliptical aperture located at a distance of 3 annuli behind the female gonopore. The copulatory area on the clitellum is characteristic in the shape of a parallel ellipse. The vector tissue is in the form of a transverse plate below the oviduct outlet. For a complete description of the external morphology, a geometric-mathematical model of the leech body form was used.

Erpobdellid leeches (Annelida, Clitellata, Hirudinida) from Tunisia: new records with the description of a new Trocheta species.

Up to now in Tunisia, freshwater Hirudinida are represented by two mainly haematophagous families: Hirudinidae and Glossiphonidae, and a predatory one: the family Erpobdellidae. The present study provides new information on the diversity and taxonomy of erpobdellid leeches. Identification was based, in addition to morphological data, on the length of sperm ducts and the lengths of ovisacs in relation to the neurosomite (ns) and on the shape and size of the male atrium. Five taxons are found. Two subspecies are reported for the first time in the country: Dina punctata punctata Johansson, 1927 and Dina punctata maroccana Nesemann and Neubert, 1994. Tunisian populations of two species, Erpobdella testacea (Savigny, 1820) and Trocheta africana Nesemann and Neubert, 1994, are described, with records of new localities. The new Trocheta tunisiana n. sp. is discovered and described in detail. Trocheta species live in springs in elevated areas while Erpobdella seem to prefer low altitude reservoirs. A comprehensive comparison of the three genera is presented. The disparity between the actual systematics and phylogeny is discussed. This study gives also a detailed distribution of the five species in the north of Tunisia with notes on ecological preference of the genus Dina. Finally a key for the determination of freshwater erpobdellid species from Tunisia is proposed.

Morphology and ultrastructure of the midgut in Piscicola geometra (Annelida, Hirudinea).

This paper presents information on the organization of the midgut and its epithelium ultrastructure in juvenile and adult specimens of Piscicola geometra (Annelida, Hirudinea), a species which is a widespread ectoparasite found on the body and gills and in the mouth of many types of fish. The analysis of juvenile nonfeeding specimens helped in the explanation of all alterations in the midgut epithelium which are connected with digestion. The endodermal portion (midgut) of the digestive system is composed of four regions: the esophagus, the crop, the posterior crop caecum, and the intestine. Their epithelia are formed by flat, cuboidal, or columnar digestive cells; however, single small cells which do not contact the midgut lumen were also observed. The ultrastructure of all of the regions of the midgut are described and discussed with a special emphasis on their functions in the digestion of blood. In P. geometra, the part of the midgut that is devoid of microvilli is responsible for the accumulation of blood, while the epithelium of the remaining part of the midgut, which has a distinct regionalization in the distribution of organelles, plays a role in its absorption and secretion. Glycogen granules in the intestinal epithelium indicate its role in the accumulation of sugar. The comparison of the ultrastructure of midgut epithelium in juvenile and adult specimens suggests that electron-dense granules observed in the apical cytoplasm of digestive cells take part in enzyme accumulation. Numerous microorganisms were observed in the mycetome, which is composed of two large oval diverticles that connect with the esophagus via thin ducts. Similar microorganisms also occurred in the cytoplasm of the epithelium in the esophagus, the crop, the intestine, and in their lumen. Microorganisms were observed both in fed adult and unfed juvenile specimens of P. geometra, which strongly suggests that vertical transmission occurs from parent to offspring.

A checklist of leech species from Poland.

In this study 47 leech species from Poland are listed. They belong to two orders, two suborders, five families and 17 genera. The checklist also includes the information about hosts, distribution in Poland and references concerning the leech species discussed in this study.

Batracobdella algira Moquin-Tandon, 1846 (Hirudinida: Glossiphoniidae)--morphometric analysis and internal morphology.

There has been very few papers on leeches of Batracobdella algira. The aim of the presently reported study was to provide the measurements and, for the first time, description and characteristics of the reproductive and digestive systems of B. algira. The material used in this study was collected from frogs, Rana saharica Boulenger, 1913, in April 1987 by H. Hotz in Béja in Tunisia (11 individuals) and by R. Ben Ahmed in 2008 from the skin of Bufo mauritanicus Schlegel, 1841 in Nabeul in Tunisia (18 individuals). The relative length of the specimens ranges between 1.48 and 4.07. The digestive system of leeches has a standard structure for the species from the family Glossiphoniidae. Male and female gonopores are separated by two annuli. Ovisacs are as long as 5 neurosomits. The characteristic features of the male reproductive system of Batracobdella are sperm ducts, which are twisted in a small area and placed near to atrium. This study will hopefully contribute to a better understanding of the host-parasite relation.

Body form of leeches (Hirudinida: Piscicolidae) parasitizing fishes.

The body form of leeches, which becomes a modeling object, is a valid ectosomatic feature. It describes borders, in which, we can find internal organs of an individual. The range of realization of the body form, proposed for different groups of leeches, characterize their life spaces. These life spaces are distinct in term of position of body of leeches to the host and to the environment. Individuals originated from the Caspian Sea, Baikal Lake, and from fresh waters of Italy, Finland, Russia, Germany and Poland. Over 1200 individuals, belonging to 11 genera were measured. The measurements have been made according to the model of the leech body form.

[Questionnaire impact on participation and autonomy (IPA)]. / Kwestionariusz wplywu na uczestnictwo i autonomie--impact on participation and autonomy (IPA).

There are many problems which can decrease the quality of life of disabled persons. It means either social condition, family life, education, vocational status, sport, recreation and psychological implications. The Impact on Participation and Autonomy Questionnaire (IPA) is a valid, reliable and acceptable measure of participation and autonomy in people with a range of conditions, especially with multiple sclerosis, spinal cord injury and rheumatoid arthritis. The psychometric properties of IPA are promising and indicate that IPA has potential to be developed further. Most respondents believe that the IPA items are easy or very easy to understand, relevant and not embarrassing. In review report the own translation of IPA into Polish language has been presented.

Biological diversity of leeches (Clitellata: Hirudinida) based on characteristics of the karyotype.

The majority of studies on leeches are related to: internal and external morphology. Fewer molecular research projects focus on molecular studies and karyology. The latter are needed to explain the evolutionary and systematic issues. Karyotypes for 22 species of Hirudinida have hitherto been determined, including: 8 species belonging to the Glossiphoniidae family, 3 species of Piscicolidae, 7 of Erpobdellidae, 1 of Haemopidae and 3 of Hirudinidae. The chromosome number vary among individual groups of leeches. Within Glossiphoniidae the chromosome diploid number ranges from 14 to 32, in Piscicolidae from 20 to 32, in Erpobdelliformes from 16 to 22, and in Hirudiniformes from 24 to 28. The karyological analyses were used to show phylogenetic relations between main groups of Hirudinida, and the diploid number of 16 was suggested to be a primitive value. This number tends to increase as the evolution progresses. The phylogeny scheme of leeches proposed by Mann shows the Glossiphoniidae as primitive to the Piscicolidae, and Hirudinidae as giving rise to the Haemopidae and Erpobdellidae. Those hypotheses are herewith confronted with morphological, molecular, karyological, ecological and behavioral data.

Leeches (Hirudinida: Piscicolidae)--parasites of Antarctic fish from Channichthyidae family.

There has hitherto been very few research projects focusing on ectoparasites of Antarctic fishes. The presently reported study provides data on the prevalence and the intensity of leeches (Hirudinida: Piscicolidae) infecting fishes. The materials were collected in December-February 1986/87 off the Elephant Island, South Georgia, Joinville Island, and South Shetlands. The following leech taxa were recorded in the Antarctic fishes of the family Channichthyidae: Trulliobdella capitis (Brinkmann, 1947); Cryobdella antarctica Epstein, 1970; Nototheniobdella sawyeri Utevsky, 1997; and Cryobdella sp. The above findings constitute new geographic records from off Elephant and Joinville Island and South Georgia.

Oogenesis in four species of Piscicola (Hirudinea, Rhynchobdellida).

Piscicola has a pair of elongated sac-shaped ovaries. Inside the ovaries are numerous small somatic cells and regularly spherical egg follicles. Each follicle is composed of three types of cells: many (average 30) germ cells (cystocytes) interconnected by intercellular bridges in clones (cysts), one intermediate cell, and three to five outer follicle cells (envelope cells). Each germ cell in a clone has one intercellular bridge connecting it to the central anucleate cytoplasmic mass, the cytophore. Each cluster of germ cells is completely embedded inside a single huge somatic follicle cell, the intermediate (interstitial) cell. The most spectacular feature of the intermediate cell is its development of a system of intracytoplasmic canals apparently formed of invaginations of its cell membrane. Initially the complex of germ cell cluster + intermediate cell is enclosed within an envelope composed of squamous cells. As oogenesis progresses the envelope cells gradually degenerate. All the germ cells that have terminated their mitotic divisions are of similar size and enter meiotic prophase, but one of the cystocytes promptly starts to grow faster and differentiates into the oocyte, whereas the remaining cystocytes withdraw from meiosis and become nurse cells (trophocytes). Numerous mitochondria, ER, and a vast amount of ribosomes are transferred from the trophocytes via the cytophore toward the oocyte. Eventually the oocyte ingests all the content of the cytophore, and the trophocytes degenerate. Little vitellogenesis takes place; the oocyte gathers nutrients in the form of small lipid droplets. At the end of oogenesis, an electron-dense fibrous vitelline envelope appears around the oocyte, among short microvilli. At the same time, electron-dense cortical granules occur in the oocyte cortical cytoplasm; at the end of oogenesis they are numerous, but after fertilization they disappear from the ooplasm. In the present article we point out many differences in the course of oogenesis in two related families of rhynchobdellids: piscicolids and glossiphoniids.

Walking index for spinal cord injury.

This review reports on the contemporary possibilities of objective evaluation of walking ability in patients with paraplegia following a spinal cord injury. Current methods of evaluation of walking function, i.e. the ASIA Classification, Functional Independence Measure (FIM), Barthel Index and Spinal Cord Independence Measure (SCIM) are described. The latest classification, known as the WISCI (Walking Index for Spinal Cord Injury) is described in detail. WISCI is the most detailed scale that is also the most sensitive to changes in the patient's walking ability compared to the other scales.

Structure of the vector tissue in piscicolid leeches (Annelida, Hirudinea, Rhynchobdellida, Piscicolidae).

Hypodermic insemination occurs in piscicolid leeches (Hirudinea, Rhynchobdellida, Piscicolidae). The spermatophore is implanted in a specialized region of the leech body, the copulatory area. Just beneath the copulatory area, there is a specialized connective tissue (vector tissue) that is considered to guide the sperm toward the ovaries. In this study, we show that the vector tissue in the four species of the genus Piscicola is composed of a mass of cells located directly beneath the copulatory area, and two thin strands extend toward the ovaries. The ultrastructure of the vector tissue has been described for the first time. Four cell types were identified, constructing the vector tissue. The envelope of this tissue is made up of extracellular fibrous matrix and two types of cells: vesicular and flat envelope cells, which are embedded within the matrix. The rest of the tissue is formed of granular and plasmatic cells. Both of these last cell types have prominent cytoplasmic projections, filled with a filamentous material. However, only granular cells have numerous small electron-dense granules in their cytoplasm. The vector tissue was described prior, during and following copulation. Sperm passes within free spaces between the granular and plasmatic cells. Characteristic vector tissue cells also occur within the ovary wall and inside the ovary lumen. This supports earlier data, which postulated that the vector tissue appears to be an outgrowth of the ovary wall.