[Incidence of osteoporosis discovered during prophylactic tests in women from the Northeastern region of Poland]. / Czestosc wystepowania osteoporozy w badaniach profilaktycznych kobiet regionu Polski pólnocno-wschodniej.

In at random well-chosen group of 803 women one passed investigation of thickness of bone of forearm or limb not dominating. All examined were divided into two groups: women before menopause and after menopause. Bone mineral structure was estimated in individuals by thickness of osseous mass--BMD. Criteria of osteoporosis was coefficient T-score, which value one accepted peaceably with recommendations of WHO. Osteoporosis steps out considerably more often in women after menopause and grows together as the years go after last menstruation.

[Risk of osteoporosis occurrence and body mass index BMI in the population of women of the Northeastern region of Poland]. / Ryzyko wystapienia osteoporozy a wskaznik masy ciala BMI w populacji kobiet regionu Polski pólnocno-wschodniej.

In group 775 of women from region of north-eastern Poland, in period before and after menopause we estimated mass of body on the ground values of coefficient BMI. Values of coefficient between 18.5 and 24.9 were accepted as normal according to WHO. In chances of stoutness we qualified the type counting coefficient WHR. At all examined one passed estimation of thickness of tissue osseous bones of forearm. In rated frequency of occurrence of osteoporosis at examined women. One ascertained, that overweight and stoutness indeed puts into influences on lowering frequencies of occurrence osteoporosis and favors the maintenance correct thickness.

[Maternal plasma and amniotic fluid interleukin-6 and tumor necrosis factor-alpha levels in imminent preterm labor]. / Stezenie interleukiny-6 i czynnika martwicy nowotworu-alpha w surowicy krwi i plynie owodniowym ciezarnych z zagrazajacym porodem przedwczesnym.

DESIGN: Our purpose was to investigate the maternal plasma and amniotic fluid interleukin-6 and tumor necrosis factor-alpha levels in women with imminent preterm labour. MATERIAL AND METHODS: The present study was designed to evaluate IL-6 and TNF-alpha levels in 93 pregnant women with threatened preterm labour and 40 women with normal pregnancy. Maternal blood samples were collected by routine forearm venipuncture at admission during routine laboratory tests. Amniotic fluid was collected through hysterectomy during caesarean delivery from women at term but not in labour and by amniotomy or hysterectomy from women with preterm labour. RESULTS: Maternal plasma and amniotic fluid IL-6 and TNF-alpha concentrations were significantly elevated in women with preterm labour (premature rupture of membranes and uterine contractions) compared to gestationally matched, non-labouring controls.

[Maternal plasma and amniotic fluid interleukin-6 levels in imminent preterm labor]. / Stezenie interleukiny-6 w surowicy krwi i plynie owodniowym ciezarnych z zagrazajacym porodem przedwczesnym.

Our purpose was to investigate the maternal plasma and amniotic fluid interleukin-6 levels in women with preterm labour. The present study was designed to evaluate IL-6 levels in 93 pregnant women with threatened preterm labour and 40 normal pregnant. Maternal blood samples were collected by routine forearm venipuncture at admission during routine laboratory tests. Amniotic fluid was collected during hysteretomy during caesarean delivery from women at term but not in labour and by amniotomy or hysteretomy from women with preterm labour. Maternal plasma and amniotic fluid IL-6 concentrations were significantly elevated in women in preterm labour (premature rupture of membranes and uterine contractions) compared to gestationally matched, non-laboring controls.

[Estimation of blood viscosity in woman at puerperium]. / Ocena lepkosci krwi u poloznic.

Marks of blood viscosity in different states of physiology and of pathology of man are well-supplied documentary evidence. One recognized also influence of blood viscosity on disclosure of some disease processes. Period pregnancy and labour joins with changes of volume and of composition of blood circulation. Authors decided to price blood viscosity in period puerperal and to compare these given to universally coming to light anaemia's woman in puerperium. Investigations one passed in groups pregnant and woman in puerperium from different degree exacerbations of anaemia. Obtained results of stickiness one achieved to morphological parameters of blood. One ascertained, that blood viscosity is less at woman in puerperium than at women pregnant and grows less together from degree anaemia's.

[Analysis of the frequency of diseases during twin pregnancy, methods of its completion and complications ascertained in newborns from personal clinical materials]. / Analiza czestosci schorzen towarzyszacych ciazy blizniaczej, sposobów jej zakonczenia i powiklan stwierdzanych u noworodków w materiale wlasnym kliniki.

One analysed course 49 of pregnancies and labours, which one took place in Clinic of Obstetrics Medical Academy in Bialystok in years 1997-1999. Most frequent complication of bigeminal pregnancy was premature labour (53.1%). Considerable percentage of gemellary pregnancies was delivered of caesarean section. In chances of pregnancies finished before term--62% and 57% in pregnancies finished in term. Indication to caesarean section was most often threatening intrauterine infection of embryo at premature departure amniotic fluid in premature delivery and complications of preeclampsia in delivery at term. Labour in operating way was more profitable for new-born child than childbirth ways of nature.

[The role of endothelium in normal pregnancy and pregnancy complicated by preeclampsia]. / Rola sródblonka naczyniowego w ciazy prawidlowej i powiklanej EPH gestoza.

Endothelial cell dysfunction is thought to play a role in preeclampsia and the reduced production by vascular endothelial cells of the antiaggregatory and vasodilatory factors is well documented. The present study was designed to evaluate endothelial cells function in preeclamptic and healthy pregnant subjects. The nitric oxide plasma concentration in women with preeclampsia was significantly lower as compared with normotensive pregnant women. A significant increase in ET concentration was found in preeclamptic women as compared with normal pregnant patients and normal non-pregnant. The plasma concentrations of von Willebrand factor were significantly increased in healthy pregnancy as compared with preeclamptic patients. The results of our study demonstrate a significant endothelial cells damage in preeclamptic patients. Whether these observations contribute to the vascular pathophysiologic features of preeclampsia remains to be proved.

[The role of nitric oxide and blood platelets in pathogenesis of preeclampsia]. / Rola tlenku azotu (NO) i plytek krwi w patogenezie EPH gestozy.

The aim of this study was to evaluate the concentration of nitric oxide and the platelet function in preeclamptic and normal pregnant women. The patients with preeclampsia had new hypertension (diastolic blood pressure consistently > or = 90 mmHg with previously lower readings), new proteinuria and generalized oedema that subsequently regressed after delivery. Blood was collected by routine forearm venipuncture before delivery. The following parameters were evaluated: nitric oxide, beta-TG and PF4. The nitric oxide plasma concentration in women with preeclampsia was significantly lower compared with normotensive pregnant women. beta-TG and PF4 concentrations were significantly increased in patients with preeclampsia. Whether these observations contribute to the vascular pathophysiologic features of preeclampsia remains to be proved.

[Alterations in the content of collagenous constituents of amniotic fluid in the course of EPH-gestosis]. / Zmiany w zawartosci skladników kolagenowych plynu owodniowego w przebiegu gestozy-EPH.

OBJECTIVES: EPH-gestosis may cause placental insufficiency and affect the metabolism of placenta and fetus. Various metabolites of amniotic fluid may reflect the fetal metabolism. DESIGN: It was decided to compare the content and some biochemical features of hydroxyproline-containing collagenous constituents in the amniotic fluid derived from normal gestations and gestations affected by EPH-gestosis. MATERIALS AND METHODS: Amniotic fluid was taken during labour (full-term gestations) by amniocentesis from 10 healthy women (control group) and 10 women with EPH-gestosis. UV-absorption spectra, protein and hydroxyproline were measured in non-dialyzed and dialyzed amniotic fluid. Hydroxyproline-containing collagenous constituents of amniotic fluid were chromatographed on Sephadex G-200. RESULTS: Statistical differences in concentrations of total and non-dialyzable hydroxyproline between normal and gestotic amniotic fluid were not found. In contrast to that, gestotic amniotic fluid contains more dialyzable hydroxyproline (6.2 mg/ml) than normal fluid (4.5 mg/ml; p < 0.05). Molecular sieving on Sephadex G-200 shows that this difference is due to higher content of low molecular collagen degradation products. CONCLUSION: Amniotic fluid, derived from pregnancies complicated by EPH-gestosis, contains higher amount of low molecular collagen degradation products than normal amniotic fluid. It is probably the result of more intensive degradation of fetal and placental collagen.

Collagenous constituents of amniotic fluid.

The amniotic fluid (AF) was fractionated by dialysis, gel filtration and SDS/PAGE, and submitted to the assay of collagenous constituents. The collagenous character of peptides and proteins of amniotic fluid was confirmed by hydroxyproline (Hyp) assay and treatment with bacterial collagenase followed by electrophoresis and gel filtration of the digestion products. It was found that AF contains collagen degradation products but the classical method of Hyp determination described by Woessner (Arch. Biochem. Biophys., 1961, 93, 440-447) gives overestimated values due to the interference with other AF components. Fractionation of AF on Sephadex G-100 column allowed to remove the interfering material and to estimate the actual Hyp content which equals to approx. 6.2 microg/ml. About 70% of Hyp was found in low molecular dialyzable products and the rest (about 30%) appears to be a constituent of nondialyzable collagenous polypeptides of the molecular mass of about 7.9-26.3 kDa. It is suggested that such collagenous polypeptides may be the products of proteolytic conversion of collagen precursor (procollagen) into the monomeric form of this protein. No high molecular forms of collagen, corresponding to alpha-subunits, were found.

[Collagen degradation products in amniotic fluid]. / Produkty degradacji kolagenu obecne w plynie owodniowym.

It was found that amniotic fluid (38-42 Hbd) contains hydroxyproline in concentration about 10 micrograms/ml. Gel filtration and dialysis demonstrated that most of hydroxyproline exists in a form of low molecular weight products. Furthermore, it was found that amniotic fluid contains a protein which eluates during gel filtration in void volume of the column. It gives a positive reaction for hydroxyproline but the absorption spectrum of such product is not characteristic for this amino acid. Furthermore, it is not digested by bacterial collagenase. It allows to conclude that amniotic fluid (38-42 Hbd) does not contain collagenous proteins. Only low molecular weight degradation products were found. Only part of them is susceptible on the action of bacterial collagenase.