Constituent-specific material behavior of soft biological tissue: experimental quantification and numerical identification for lung parenchyma.

In this study, we present a method to experimentally quantify and numerically identify the constituent-specific material behavior of soft biological tissues. This allows the clear identification of the individual contributions of major load-bearing constituents and their interactions in the constitutive law. While the overall approach is applicable for many tissues, here it will be presented for the identification of a sophisticated constituent-specific material model of viable lung parenchyma. This material model will help to better model the effects of various lung diseases that feature altered fiber content in the lungs, such as emphysema or fibrosis. To experimentally quantify the mechanical properties of collagen, elastin, collagen-elastin-fiber interactions, and ground substance, we examined 18 collagenase and elastase treated rat lung parenchymal slices. The mechanical contributions of the collagen and elastin fibers in the living tissue were inferred from uniaxial tension tests comparing the behavior before and after the selective digestion of the respective fibers. In order to also obtain the mechanical influence of the ground substance, we consecutively treated the samples with both proteases. Collagen and elastin fibers are morphologically interconnected. Thus, a mechanical interaction between these fibers appears likely, but has not yet been experimentally verified. In this paper, we propose an experimental method to quantitatively assess the mechanical behavior of these collagen-elastin-fiber interactions. Based on our experiments, we have identified individual material models within a nonlinear continuum mechanics framework for each load-bearing component via an inverse analysis. The proposed constituent-specific material law can be incorporated into computational models of the respiratory system to simulate and even predict the behavior and alteration of the individual constituents and their effect on the whole respiratory system during normal and artificial breathing, in particular in the case of diseases that alter the fibers in the tissue.

A viscoelastic nonlinear compressible material model of lung parenchyma - Experiments and numerical identification.

Characterizing material properties of lung parenchyma is essential in order to describe and predict the mechanical behavior of the lung in health and disease. Hence, we aim to identify the viscoelastic constitutive behavior of viable lung parenchyma with a particular focus on the nonlinear, compressible, and frequency-dependent material properties. To quantify the viscoelastic material behavior of rat lung parenchyma experimentally, we performed uniaxial tension tests with different frequencies, including the whole range of physiological frequencies, in combination with full-field displacement measurements (a total of 120 tests on 30 samples of 5 rats). By means of these experimental measurements, we identified the material parameters of two viscoelastic material models applicable to large three-dimensional deformations, i.e., the standard linear solid model and the model of fractional viscoelasticity. Our aim is to identify one set of material parameters that describes the whole range of physiological frequencies; therefore, we utilized a coupled inverse analysis, which equally incorporates all different tensile tests performed on one sample. The model most suitable for the description of the viscoelastic, nonlinear, and compressible material behavior of viable rat lung parenchyma is the strain energy function [Formula: see text] in combination with the model of fractional viscoelasticity (τ=0.06454s,α=0.5378, and ß=1.856). This material model was validated to describe the complex nonlinear and compressible viscoelastic material behavior of lung parenchyma and can be utilized in finite element simulations of the whole range of physiological frequencies. Based on this model, it will be possible to quantify the stresses and strains of lung tissue during spontaneous and artificial breathing more reliable in the future.

A coupled approach for identification of nonlinear and compressible material models for soft tissue based on different experimental setups - Exemplified and detailed for lung parenchyma.

In this paper, a coupled inverse analysis is proposed to identify nonlinear compressible hyperelastic material models described by two sets of experiments. While the overall approach is applicable for different materials, here it will be presented for viable lung parenchyma. Characterizing the material properties of lung parenchyma is essential to describe and predict the mechanical behavior of the respiratory system in health and disease. During breathing and mechanical ventilation, lung parenchyma is mainly subjected to volumetric deformations along with isochoric and asymmetric deformations that occur especially in diseased heterogeneous lungs. Notwithstanding, most studies examine lung tissue in predominantly isochoric tension tests. In this paper, we investigate the volumetric material behavior as well as the isochoric deformations in two sets of experiments: namely, volume-pressure-change experiments (performed with 287 samples of 26 rats) and uniaxial tension tests (performed with 30 samples of 5 rats). Based on these sets of experiments, we propose a coupled inverse analysis, which simultaneously incorporates both measurement sets to optimize the material parameters. Accordingly, we determine a suitable material model using the experimental results of both sets of experiments in one coupled identification process. The identified strain energy function with the corresponding material parameters [Formula: see text] is validated to model both sets of experiments precisely. Hence, this constitutive model describes the complex volumetric and isochoric nonlinear material behavior of lung parenchyma. This derived material model can be used for nonlinear finite element simulations of lung parenchyma and will help to quantify the stresses and strains of lung tissue during spontaneous and artificial breathing; thus, allowing new insights into lung function and biology.

Experimental characterization and model identification of the nonlinear compressible material behavior of lung parenchyma.

The mechanical properties of lung parenchyma are essential both in lung function and biology; consequently, experimental methods are developed to describe the mechanical behavior of lung parenchyma. During breathing and mechanical ventilation, volume change is the physiologically dominating deformation mode of lung parenchyma; nevertheless, most studies examine lung tissue in mainly isochoric tension tests. In this paper, a novel experimental method for the quantification of the compressible material behavior at high volume changes of viable lung parenchyma is proposed. This volume-pressure-change experiment quantifies the pressure and corresponding volume change of lung parenchyma slices. For the characterization of the compressible constitutive properties over the whole physiological pressure range, we combine this newly derived experiment with uniaxial tension tests. The experimental results of both the volume-pressure-change experiments, for which 287 samples were examined, and the uniaxial tension tests, which were performed on 36 specimens, are presented. The resulting measurements are utilized to optimize the material parameters of one suitable hyperelastic strain-energy function describing the nonlinear compressible material behavior of viable lung parenchyma. The derived constitutive model can be used for simulations of lung parenchyma, and will help to quantify the strains and stresses of lung tissue during normal breathing and mechanical ventilation.

Beam finite-element model of a molecular motor for the simulation of active fibre networks.

Molecular motors are proteins that excessively increase the efficiency of subcellular transport processes. They allow for cell division, nutrient transport and even macroscopic muscle movement. In order to understand the effect of motors in large biopolymer networks, e.g. the cytoskeleton, we require a suitable model of a molecular motor. In this contribution, we present such a model based on a geometrically exact beam finite-element formulation. We discuss the numerical model of a non-processive motor such as myosin II, which interacts with actin filaments. Based on experimental data and inspired by the theoretical understanding offered by the power-stroke model and the swinging-cross-bridge model, we parametrize our numerical model in order to achieve the effect that a physiological motor has on its cargo. To this end, we introduce the mechanical and mathematical foundations of the model, then discuss its calibration, prove its usefulness by conducting finite-element simulations of actin-myosin motility assays and assess the influence of motors on the rheology of semi-flexible biopolymer networks.