Citizen scientists filling knowledge gaps of phosphate pollution dynamics in rural areas.

In situ monitoring is fundamental to manage eutrophication in rivers and streams. However, in recent decades, the frequency and spatial coverage of regulatory monitoring have often been reduced due to funding and infrastructure limitations. This reduction has made it impossible to provide adequate coverage for most water bodies. In this study, trained citizen scientists filled spatial and temporal gaps in agency monitoring across a major catchment in rural England. By integrating data from citizen scientists, regulatory agencies, and the local water company, it was possible to demonstrate the opportunities for hypothesis-based citizen scientist monitoring to identify continuous and event-driven sources of phosphate pollution. Local citizen scientists effectively covered important spatial gaps, investigating river conditions both upstream and downstream of suspected pollution point sources, improving the identification of their temporal dynamics. When combined with long-term monitoring data from regulatory agencies, it became possible to identify areas within the catchment that exhibited increased phosphate concentrations during periods of low river discharge (summer). Inter-annual trends and anomaly detection suggested that continuous pollution sources dominated over event-driven sources in many sub-basins, allowing for the prioritisation of mitigation actions. This study highlights the opportunity for citizen scientists to fill gaps in regulatory monitoring efforts and contribute to the improved management of eutrophication in rural catchments.

Community monitoring of coliform pollution in Lake Tanganyika.

Conventional water quality monitoring has been done for decades in Lake Tanganyika, under different national and international programs. However, these projects utilized monitoring approaches, which were temporally limited, labour intensive and costly. This study examines the use of citizen science to monitor the dynamics of coliform concentrations in Lake Tanganyika as a complementary method to statutory and project-focused measurements. Persons in five coastal communities (Kibirizi, Ilagala, Karago, Ujiji and Gombe) were trained and monitored total coliforms, faecal coliforms and turbidity for one year on a monthly basis, in parallel with professional scientists. A standardized and calibrated Secchi tube was used at the same time to determine turbidity. Results indicate that total and faecal coliform concentrations determined by citizen scientists correlated well to those determined by professional scientists. Furthermore, citizen scientist-based turbidity values were shown to provide a potential indicator for high FC and TC concentrations. As a simple tiered approach to identify increased coliform loads, trained local citizen scientists could use low-cost turbidity measurements with follow up sampling and analysis for coliforms, to inform their communities and regulatory bodies of high risk conditions, as well as to validate local mitigation actions. By comparing the spatial and temporal dynamics of coliform concentrations to local conditions of infrastructure, population, precipitation and hydrology in the 15 sites (3 sites per community) over 12 months, potential drivers of coliform pollution in these communities were identified, largely related to precipitation dynamics and the land use.

Using citizen science to understand river water quality while filling data gaps to meet United Nations Sustainable Development Goal 6 objectives.

This study investigates water quality along the river Liffey in Dublin city with the help of citizen scientists, including the community of river users such as paddle boarders and those accessing the river from the bank. The primary objective was to evaluate water quality near sources of pollution observed by citizens, while filling data gaps for the United Nations (UN) Sustainable Development Goal (SDG) 6, Indicator 6.3.2. The participants used field chemistry kits to measure nitrate (NO3-N) and phosphate (PO4-P) at 19 locations on a monthly basis over the course of nine months, recording the results on a smartphone app. 10% of nitrate samples were indicative of low quality water values while 35.6% of phosphate samples were indicative of low quality water. Rainfall over the study period was analysed to investigate the impact of run-off from rainwater on the river. Results indicated that excessive rainfall was not a factor in lower water quality in this area. Citizen scientists' observational notes and photographs entered onto the database, with accompanying test results were key to highlighting pollution sources at specific locations which correlated with high levels of nitrate and phosphate resulting in low quality water. Land use was a factor in these areas of recent housing development indicating possible domestic misconnections. Citizen scientist data has the potential to fulfil UN SDG 6, in contributing to Indicator 6.3.2 while detecting contamination.

Mechanisms Underlying the Anti-Suicidal Treatment Potential of Buprenorphine.

Death by suicide is a global epidemic with over 800 K suicidal deaths worlwide in 2012. Suicide is the 10th leading cause of death among Americans and more than 44 K people died by suicide in 2019 in the United States. Patients with chronic pain, including, but not limited to, those with substance use disorders, are particularly vulnerable. Chronic pain patients have twice the risk of death by suicide compared to those without pain, and 50% of chronic pain patients report that they have considered suicide at some point due to their pain. The kappa opioid system is implicated in negative mood states including dysphoria, depression, and anxiety, and recent evidence shows that chronic pain increases the function of this system in limbic brain regions important for affect and motivation. Additionally, dynorphin, the endogenous ligand that activates the kappa opioid receptor is increased in the caudate putamen of human suicide victims. A potential treatment for reducing suicidal ideation and suicidal attempts is buprenorphine. Buprenorphine, a partial mu opioid agonist with kappa opioid antagonist properties, reduced suicidal ideation in chronic pain patients with and without an opioid use disorder. This review will highlight the clinical and preclinical evidence to support the use of buprenorphine in mitigating pain-induced negative affective states and suicidal thoughts, where these effects are at least partially mediated via its kappa antagonist properties.

In vivo and in vitro Characterization of a Partial Mu Opioid Receptor Agonist, NKTR-181, Supports Future Therapeutic Development.

Mu opioid receptor (MOPr) agonists are well-known and frequently used clinical analgesics but are also rewarding due to their highly addictive and often abusive properties. This may lead to opioid use disorder (OUD) a disorder that effects millions of people worldwide. Therefore, novel compounds are urgently needed to treat OUD. As opioids are effective analgesics and OUD often occurs in conjunction with chronic pain, these novel compounds may be opioids, but they must have a low abuse liability. This could be mediated by diminishing or slowing blood-brain barrier transport, slowing target receptor binding kinetics, and showing a long half-life. NKTR-181 is a PEGylated oxycodol and a MOPr agonist that has slowed blood-brain barrier transport, a long half-life, and diminished likeability in clinical trials. In this study, we examined the signaling and behavioral profile of NKTR-181 in comparison with oxycodone to determine whether further therapeutic development of this compound may be warranted. For this preclinical study, we used a number of in vitro and in vivo assays. The signaling profile of NKTR-181 was determined by the electrophysiological assessment of MOPr-Ca2+ channel inhibition in the nociceptive neurons of rodent dorsal root ganglia. Heterologous cell-based assays were used to assess biased agonism and receptor trafficking. Different rodent behavioral models were used to define the NKTR-181-induced relief of effective and reflexive nociception and drug-seeking behavior as assessed by an intravenous self-administration (IVSA) of NKTR-181. We found that NKTR-181 and oxycodone are partial agonists in G-protein signaling and Ca2+ channel inhibition assays and promote limited MOPr desensitization. However, NKTR-181 inhibits Ca2+ channels by a different mechanism than oxycodone and induces a different pattern of arrestin recruitment. In addition, NKTR-181 has a slower receptor on-rate and a slower rate of Ca2+ channel coupling than oxycodone. This signaling profile is coupled with a slower onset of antinociception and limited drug-seeking behavior in comparison with oxycodone. Together with its known long half-life and slow blood-brain barrier transport, these data suggest that NKTR-181 could be further studied as a pharmacotherapeutic treatment modality for OUD.